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排序方式: 共有2293条查询结果,搜索用时 15 毫秒
1.
大鼠脑内儿茶酚胺类递质及其代谢物的同时提取及反向高效液相测定法 总被引:3,自引:0,他引:3
目的建立一种操作简便、高效的测定脑组织中去甲肾上腺素(NA)、肾上腺素(AD)、多巴胺(DA)、3,4二羟基苯乙酸(DOPAC)及高香草酸(HVA)的方法,为有关药物作用机理的研究提供实验手段.方法以有机溶剂提取,高效液相色谱--电化学检测器(HPLC-EC)测定大鼠纹状体、皮质、下丘脑NA、AD、DA、DOPAC及HVA的含量.结果测得NA、AD、DA、DOPAC及HVA的绝对回收率依次分别为:80.3%±12.4%,86.5%±14.3%,90.3%±12.1%,89.5%±17.2%、87.6%±[13].2%.线性范围0.2~20ng,批内与批间变异系数分别小于8%及10%.结论该方法具有简便、迅速、回收率较高的特点,便于实验室应用. 相似文献
2.
Geoffrey Thiele Molly Bicak Helen Grierson Patrick Lai David Purtilo 《Journal of immunological methods》1987,100(1-2):249-259
An enzyme-linked immunosorbent assay (ELISA) was used to measured IgG antiboody titers againt a synthetic peptide whose sequence was derived from the glycine-alanine repeating region of Epstein-Barr virus nuclear associated antigen 1 (EBNA-1). Antibody titers were determined in sera from 15 normal subjects, sera from 21 normal male siblings of X-linked lymphoproliferative syndrome (XLP) patients, from 20 XLP patients comprising a total of 42 samples, and ten samples before and ten samples after gamma-globulin therapy in ten patients with XLP. Data analysis demonstrated that while there are differences between the ELISA and ACIF, they appear to measure a similar response as demonstrated by their correlation coefficient (0.77) and the GMT to EBNA observed by both methods. No cross-reactivity of cytomegalovirus antibodies to the EBNA-1 peptide was observed by immunobv using adsorption against AD-169 infected MRC-5 cells.. However, non-specific binding was observed if samples were not pre-incubated in a 10% goat serum PBS-Tween 20 solution. This pre-treatment removed the non-specific binding that falsely elevated GMT in approximately 15% of both normal and XLP samples in ELISA. The ELISA system appears to be a sensitive, reproducible and objective test that may be useful for assessing the antibody responses of patients to the EBNA-1 protein. 相似文献
3.
A. I. Tew 《Medical & biological engineering & computing》1988,26(1):68-74
For some disabled people pointing provides a more convenient means of communication and control than the use of switches.
The quadriplegic who retains good control of head movement can be provided with a number of input alternatives, but no existing
system meets all the requirements of a general-purpose electronic pointing device. Consequently the Oxford Optical Pointer
has been developed. The principle by which it converts relative direction into analogue electrical signals is described and
its application as a head-mounted device for controlling an electric wheelchair is presented. 相似文献
4.
麻醉剂量羟甲芬太尼对大鼠脑内单胺递质及其代谢物含量的影响金昔陆1唐琴梅金文桥周德和李桂芬池志强(中国科学院上海药物研究所,上海200031)羟甲芬太尼(ohmefentanyl,OMF)是一种新的高选择性高亲和力μ阿片受体激动剂,在动物中能产生麻醉作... 相似文献
5.
空管药物疗法治疗牙髓病和根尖周病疗效观察 总被引:1,自引:0,他引:1
用SMTD复合药物对牙髓病和根尖周病实施空管药物疗法。78例103颗获得完整随访资料患牙经两年观察,92.2%治愈率。文章介绍了治疗方法,讨论了空管药物疗法的愈合机理、优点、失败原因及其预防措施。 相似文献
6.
Flow Cytometric Analysis of Lymphocyte Subsets of Mice Maintained on an Ethanol-Containing Liquid Diet 总被引:1,自引:0,他引:1
Linda Hsiung Joseph Wang Carl Waltenbaugh 《Alcoholism, clinical and experimental research》1994,18(1):12-20
Alcoholic patients often have impaired immune function, yet little is known about the precise mechanism(s) of this impairment. We have previously shown that ethanol consumption by mice alters copolymer-specific humoral and cellular immune responses. In this study, we asked whether alcohol consumption by mice would phenotypically alter lymphocyte populations. Female C57BL/6 mice were fed a nutritionally complete liquid diet containing 35% ethanol-derived calories for up to 8 days. As controls, mice either were fed a liquid control diet that isocalorically substitutes sucrose for ethanol or remained on a standard solid diet and water ad libitum. Although mice fed ethanol-containing liquid or pair-fed control liquid diets have decreased numbers of spleen cells compared with solid diet controls, only the ethanol-containing diet allowed normally nonresponder C57BL/6 spleen cells to make antibody responses to the poly(Glu50Tyr50) synthetic copolymer antigen. Flow cytometric analysis of splenic lymphocyte populations of mice on the ethanol-containing diet shows an increase in the relative proportion of T-lymphocytes as compared with mice on either solid or liquid control diets. No such change is seen for either B-cell or natural killer cell populations in these same mice. Both liquid control and liquid ethanol diets caused a slight decrease in the CD4:CD8 ratios of splenic T-lymphocytes. We see the relative percentage of T-cells bearing the αβ-cell receptor (TcR) increases in the spleens of liquid ethanol diet mice; a smaller increase TcRαβ usage is seen in the spleens of liquid control mice, compared with solid diet mice. Flow cytometric analysis shows that little, if any, difference exists in TcRγδ expression between the liquid ethanol and either the liquid control or solid diet groups. Preliminary analysis of TcRαβ subsets suggest that ethanol increases the percentage of T-cells expressing Vβ5 and Vβ8, and decreases the percentage of Vβ11 expressing cells. These findings suggest that, in addition to modifying the immune response, ethanol alters the phenotypic expression of lymphocyte subsets. 相似文献
7.
Open mesh versus non-mesh repair of groin hernia meta-analysis of randomized trials leased on individual patient data 总被引:1,自引:1,他引:0
Abstract
Background. The EU Hernia Trialists Collaboration was established to provide reliable evaluation of newer methods of groin hernia repair.
It involved 70 investigators in 20 countries.
Materials and methods. Twenty eligible trials (5016 participants) of open mesh vs. non-mesh groin hernia repair were identified. Meta-analysis was
performed using raw individual patient data where possible.
Results. Fewer hernia recurrences were reported after mesh repair. There were no clear differences between mesh and non-mesh groups
in complications. Overall, those in the mesh groups had a shorter hospital stay, quicker return to usual activities and less
frequent persisting pain, but individual trial results varied.
Conclusions. The review provides strong evidence that open mesh repair is associated with a reduction in the risk of recurrence of between
50% and 75%. There is also some evidence of quicker recovery and of lower rates of persisting pain following open mesh repair.
Electronic Publication 相似文献
8.
Kurt Weising Dieter Kaemmer Jörg T. Epplen Franz Weigand Mohan Saxena Günter Kahl 《Current genetics》1991,19(6):483-489
Summary The ascomycete fungus Ascochyta rabiei, an important pathogen of the grain legume crop chickpea (Cicer arietinum L.) in the Mediterranean region, has not been adequately characterized in molecular terms. We therefore used DNA fingerprinting, with synthetic oligodeoxynucleotides complementary to simple repetitive sequences, to pathotype different isolates of the fungus. Six single-spored A. rabiei isolates were first categorized using a host differential set of nine chickpea genotypes. Seedlings were inoculated under controlled environmental conditions, and disease severity was recorded 9 days after inoculation. DNA was extracted from in vitro-grown mycelia of the six purified fungal isolates, restricted with EcoRI, HinfI, MboII and TaqI, and fingerprinted with radiolabeled (GATA)4, (GTG)5, (CA)8, and (TCC)5, respectively. High levels of polymorphism were detected with optimal enzyme/probe combinations that allow one to discriminate between the isolates. The potential of DNA fingerprinting with simple repetitive sequences can thus be expanded to the identification of fungal races and pathotypes. The characterization of the geographic distribution and genetic variability of pathotypes will facilitate the selection of suitable host cultivars to be grown in specific regions. 相似文献
9.
Donald A. Czech 《Physiology & behavior》1982,29(6):1179-1181
An casuy constructed and inexpensive drop-sensing drinkometer is described, which incorporates an integrated circuit photodetector. It provides a reliable and convenient means of automatically monitoring fluid intake in a number of experimental situations. The animal is undisturbed and isolated from the electrical system components. 相似文献
10.
Tsutomu Gomi Kichirou Koshida Tosiaki Miyati Jun Miyagawa Hiroshi Hirano 《Journal of digital imaging》2006,19(4):362-370
The purpose of this work was to compare direct and indirect detectors in terms of their system linearity, presampled modulation
transfer function (MTF), Wiener spectrum (WS), noise equivalent quanta (NEQ), and power spectrum. Measurements were made on
two flat-panel detectors, GE Revolution XR/d (indirect) and Shimadzu Safire (direct) radiographic techniques. The system linearity
of the systems was measured using a time-scale method. The MTF of the systems was measured using an edge method. The WS of
the systems was determined for a variable range of exposure levels by two-dimensional Fourier analysis. The NEQ was assessed
from the measured MTF, WS, and estimated ideal signal-to-noise ratios. Power spectrum analyzed the chest phantom within artificial
lesions. System linearity was excellent for the direct systems. For the direct system, the MTF was found to be significantly
higher than that for the indirect systems. For the direct system, the WS was relatively uniform across all frequencies. In
comparison, the indirect system exhibited a drop in the WS at high frequencies. At lower frequencies, the NEQ for the indirect
system was noticeably higher than for the direct system. Power spectrum for the direct system was relatively flat and similar
to that for white noise. The indirect system exhibited significant reduction at high spatial frequencies. In general, the
direct systems exhibit improved image quality over indirect systems at comparable exposure dose. 相似文献