肺鳞癌间质毛细血管基底膜Ⅳ型胶原免疫组化研究

本文应用Ⅳ型胶原免疫组化染色对２４例肺鳞癌间质毛细血管基底膜进行研究。结果在明：肺鳞癌间质毛细血管基底膜依据染色结果可分为３种类型：Ⅰ型（基底膜呈连续线状），Ⅱ型（有缺损），Ⅲ型（缺如）。高分化鳞癌间质毛血管基底膜以Ⅰ型为主（９／１３）、低分化者５例均为Ⅲ型。结果初步证明：肺鳞癌间质毛细血管基底膜存在不同程度缺损，其损伤程度与组织分化程度相关。并认为肺鳞癌间质毛细血管基底膜缺损与肿瘤转移有密切关系。     

气功对冠心病人CM_5ST段/心率斜率的影响

37名冠心病患者随机分为气功治疗组(22人)和对照组(15人)。气功治疗组接受12周动功治疗,对照组接受安慰剂治疗,治疗前后均测定CM_5 ST段/心率斜率。结果发现动功治疗组CM_5 ST段/心率斜率减少,而对照组不变或增大,两组有显著性差异,ST段下移及常规分级运动试验其它指标在两组间无显著差异。表明CM_5ST段/心率斜率是一个敏感的心肌缺血指标。     

Effect of antispermatogenic compound CDRI-84/35 on marker enzymes of rat testis cells : A study on site of action

Marker enzymes of Sertoli and germ cells were estimated to study the mechanism of action of antispermatogenic compound CDRI 84/35 in adult male rat testis. Animals were killed after 22, 41, and 64 days of treatment with antispermatogenic dose of CDRI 84/35 in order to evaluate the effect of the compound on spermatid, spermatocyte, and spermatogonial stages, respectively. Studies were also extended to a recovery period of 90 days. Results indicate a direction action of the compound on germ cells, with no apparent effect on Sertoli cells. Studies also show a massive depletion of postmeiotic germ cells after the treatment, with some damage to premeiotic germ cells as well. Reversibility of the compound was partial, with the marker enzymes of pre- and postmeiotic germ cells not being restored to control levels after withdrawal of treatment.     

In-vitro synthesis of steroid hormones in corpora lutea from normal women and women treated with 300 micrograms norethisterone.

The in-vitro oestradiol (E2) and progesterone (P) production by corpora lutea (CL) obtained at sterilization from 30 untreated women and 43 women treated with norethisterone (NET) 300 micrograms daily was measured. The CL were obtained at different stages of the luteal phase in the untreated women [luteinizing hormone (LH) 0 to +3, n = 7; LH +4 to +7, n = 7; LH +8 to +11, n = 9; LH +12 to menses, n = 7] and on days LH +8 to +11 or cycle days 22 to 26 in the NET-treated women. In the treated women, four types of ovarian reaction were identified. Four women showed ovarian reaction Type A (completely inhibited ovarian activity), 14 women Type B (marked follicular activity, but no luteal function), 12 women Type C (normal follicular activity, followed by insufficient luteal function) and 13 women Type D (apparently normal follicular and luteal activity). The CL were incubated in Eagle's medium with and without stimulation by human chorionic gonadotrophin (HCG) for 2 and 4 h. In the untreated women, P and E2 production increased significantly with both incubation time and stimulation by HCG throughout the luteal phase, except in the late luteal phase (LH +12 to menses) where P increased (P less than 0.01) only after 4 h stimulation by HCG. The maximal production of P was found after 4 h incubation with HCG stimulation of CL tissue in the early-mid luteal phase (LH +4 to +7).(ABSTRACT TRUNCATED AT 250 WORDS)     

视网膜眼电图对DMD/BMD携带者的检测价值探讨

目的研究杜氏／贝氏进行性肌营养不良（DMD／BMD）携带者视网膜眼电图（ERG）的改变，探讨ERG对DMD／BMD携带者的诊断意义。方法对22个基因突变类型明确的DMD／BMD家系．用定量PCR法结合系谱分析将家系中女性成员分成肯定携带者和非携带者，对患者和家系女性成员进行详细的眼科检查和ERG检测，并用ERG国际测量标准记录结果。结果22名患者中17名出现异常ERG改变；11名肯定携带者中5名出现ERG异常，14名非携带者无ERG改变，两组无重叠。结论ERG是一项对DMD／BMD诊断非常敏感和特异的检查，而ERG对携带者的诊断也具有一定的临床意义。     

具有骨诱导活性的仿生骨基质材料的制备

目的将一种具有与骨形态发生蛋白2相似骨诱导活性的多肽p24共价结合于改性聚（丙交酯-co-乙交酯）基质材料上，以制备出具有骨诱导活性的仿生骨基质材料。方法将活性多肽p24通过交联剂共价结合于改性PLGA材料上作为实验组；以未加交联剂多肽溶液和材料简单混合反应为对照组，通过X射线光电子光谱法和扫描电镜检测交联情况；同时对两组材料进行钙离子吸附实验，初步评价其仿生矿化能力。结果XPS检测结果表明，实验组及对照组材料表面均已结合硫元素，两者含有硫元素含量分别为1.50％及0.09％；钙离子吸附实验结果表明，12h及24h时实验组材料的钙离子吸附量分别为0，126mg及0．231mg；12h及24h时对照组材料的钙离子吸附量分别为0．053、0．102mg。多肽交联之材料较混合之材料的钙离子吸附能力显著增强。结论在改性PLGA三嵌段材料上固定了BMP2活性多肽，为以后发挥其骨诱导活性修复骨缺损奠定了良好基础。     

DIALYSIS AND THE ENVIRONMENT: COMPARING HOME AND UNIT BASED HAEMODIALYSIS

Dialysis, with its high dependence upon technology, disposable products and transport requirements, presents an unusual perspective for environmental management. This study considers and compares the environmental aspects arising from the provision of haemodialysis (HD) in the hospital and home setting. Resource items were measured at two levels of aggregation — unit and individual patient. Unit level items applied to common resources used for HD and require apportioning appropriately with patient level items that could be attributable to individuals. The data was measured in standard units such as hours and number of treatments or apportioned appropriately. With equivalent emissions calculated as CO₂ annually per patient for standard HD. The findings indicate that HD in the home offers a net reduction in CO₂ emissions per patient annually compared to hospital based HD, and provides an overview of how healthcare provision and the use of resources can be measured, enabling refinement in environmental management plans.     

浙江人群幽门螺杆菌cagA/vacA优势基因型和不同基因型混合感染的调查

目的　了解消化性溃疡和慢性胃炎患者感染的幽门螺杆菌 (Hp)cagA vacA优势基因型及不同基因型Hp感染、混合感染与疾病的关系。 方法　选择胃窦、胃体双份活检标本均培养出Hp的 42例慢性胃炎 (CG)和 3 6例消化性溃疡 (PU )患者作为研究对象 ,采用聚合酶链反应 (PCR)检测156份Hp分离株的cagA基因、vacA基因的信号区 (s)和中间区 (m )亚型 ,分析Hp基因型及多株Hp混合感染在CG和PU中的分布。结果　胃窦标本cagA基因的检测中 78例患者中有 75例 (96.2 % )为cagA阳性 ,相应的胃体标本中 ,76例患者 (97.4% )为cagA阳性 ,有 1例 (1.3 % )患者胃窦、胃体检出cagA状态不一的Hp混合菌株。在胃窦标本的vacA基因分型中 ,s1a m1、s1a m2、s1a m1b、s1a m1b m2 4种vacA基因型在 78例患者中所占比例分别为 6.4% (5 78)、55.1% (43 78)、2 6.9% (2 1 78)和 1.3 % (1 78) ,多株混合感染为 3 .8% (3 78) ;而相应的胃体标本中 ,前述四种vacA基因型所占比例依次为 6.4% (5 78)、53 .8% (42 78)、2 5.6% (2 0 78)和 3 .8% (3 78) ,多株混合感染为 5.1% (4 78)。cagA+ s1a m2和cagA+ s1a m1b在胃窦标本中占 51.3 % (40 78)和 2 6.9% (2 1 78) ,而在相应的胃体标本中占 52 .6% (41 78)和 2 5.6% (2 0 78)。少量胃窦、胃体     

前列腺液中尿酸引起ⅢB前列腺炎84例报告

目的 :探讨前列腺液 (EPS)中尿酸 (UA)与前列腺素E2 (PGE2 )浓度与慢性盆痛症状之间的相互关系 ,以及UA引发ⅢB前列腺炎的可能机制。方法 :按国际慢性前列腺炎分类诊断标准诊断 ,将 84例慢性前列腺炎患者分为两组 ,即ⅢA组 39例 ;ⅢB组 4 5例 ;正常对照 (对照组 ) 13例。分别进行国际前列腺炎症状评分 (CP SI)和检测EPS中白细胞数、pH值、UA和PGE2 浓度。结果 :①ⅢB组患者慢性盆痛症状评分 (CPSI P)高于ⅢA组 ,EPS中UA浓度高于ⅢA组和正常对照组 ,pH值低于ⅢA和正常对照组 ,均差异有显著性意义 (均 P <0 .0 5 )。②ⅢB组和ⅢA组的PGE2 平均浓度高于正常对照组 ,差异有显著性意义 (P <0 .0 5 ) ,但是ⅢA组和ⅢB组间差异无显著性意义 (P >0 .0 5 )。CPSI P与EPS中的UA ,PGE2 之间存在正相关 (P <0 .0 1)。结论 :ⅢB组患者EPS中高浓度的UA激活环氧化酶 ,使局部的PGE2 升高 ,从而引起慢性盆痛症状 ,其严重程度与EPS中的UA、PGE2 浓度呈正比。同时PGE2 又使UA更容易渗入EPS ,形成UA和PGE2 之间的恶性循环 ,使ⅢB前列腺炎迁延不愈。