Evidence for Elizabethkingia anophelis Transmission from Mother to Infant,Hong Kong

Elizabethkingia anophelis, recently discovered from mosquito gut, is an emerging bacterium associated with neonatal meningitis and nosocomial outbreaks. However, its transmission route remains unknown. We use rapid genome sequencing to investigate 3 cases of E. anophelis sepsis involving 2 neonates who had meningitis and 1 neonate’s mother who had chorioamnionitis. Comparative genomics revealed evidence for perinatal vertical transmission from a mother to her neonate; the 2 isolates from these patients, HKU37 and HKU38, shared essentially identical genome sequences. In contrast, the strain from another neonate (HKU36) was genetically divergent, showing only 78.6% genome sequence identity to HKU37 and HKU38, thus excluding a clonal outbreak. Comparison to genomes from mosquito strains revealed potential metabolic adaptations in E. anophelis under different environments. Maternal infection, not mosquitoes, is most likely the source of neonatal E. anophelis infections. Our findings highlight the power of genome sequencing in gaining rapid insights on transmission and pathogenesis of emerging pathogens.     

重庆西永垃圾转运站工程设计

介绍了重庆西永垃圾转运站的建筑背景、工程概况及工程总体设计方案,具体阐述了转运工艺、设备配置、车间工艺布置、除臭、渗沥液处理等设计内容,并论述了该项目特点及创新性。     

Neuromuscular control and hop performance in youth and adult male and female football players

ObjectivesTo compare neuromuscular control and hop performance between youth and adult male and female football players.DesignCross-sectional study.Participants119 youth players (13–16 years; 68 males) and 88 adult players (17–26 years; 44 males).Main outcome measuresNeuromuscular control assessed with drop vertical jump (DVJ) and tuck jump assessment (TJA). Hop performance assessed with single-leg hop for distance and side hop.ResultsAdult females had smaller normalized knee separation distances (NKSD) during DVJ at initial contact (77.9 ± 18.5 vs. 86.1 ± 11.0, p = 0.010) and at maximum knee flexion (59.7 ± 23.4 vs.74.1 ± 18.1, p = 0.001) compared to youth females. TJA revealed more technique errors in youths compared to adults (males 10 (8–11) vs. 8 (7–10); females 11 (9–12) vs. 9 (8–11), p < 0.05). Youths demonstrated inferior hop performance (males single-leg hop 142 ± 18 vs. 163 ± 17, side hop 41 ± 12 vs. 52 ± 12, p < 0.001; females side hop 32 ± 10 vs. 38 ± 14, p < 0.05).ConclusionsYouth players demonstrated reduced neuromuscular control during TJA and inferior hop performance compared to adult players. Adult female players demonstrated greater knee valgus during DVJ compared to youth female players.     

新生儿静脉血与脐带血及孕妇静脉血HBV病原学标记物相关性研究

目的 探讨慢性HBV感染孕妇所生新生儿脐带血与静脉血HBV标志物状况的一致性和相关性,以及与孕妇HBV感染标志物的相关性.方法 以HBsAg、HBeAg双阳性且HBV DNA＞1 ×105拷贝/ml孕妇及新生儿为研究对象,孕妇分娩前采集静脉血,新生儿于注射乙肝免疫球蛋白、乙肝疫苗前采集静脉血.在清洁和去除脐带表面污染血液,并用酒精消毒后,用注射器采集脐带血.HBsAg、抗HBs、HBeAg、抗HBe采用雅培微粒子化学发光法(美国雅培公司试剂,Abbott Architac i2000)检测,HBV DNA含量经COBAS TagMan HBV DNA定量检测仪检测.结果 共入组孕妇383例及所生新生儿,静脉血和脐带血HBsAg的阳性检出率分别为61.2％和63.9％,HBeAg阳性检出率分别为83.2％和83.5％,HBV DNA阳性检出率分别为56.0％和59.4％,静脉血和脐带血之间均有一致性.静脉血和脐带血间HBsAg、HBeAg和HBV DNA含量的相关性具有统计学意义(r=0.766、0.857、0.692,P＜0.000).新生儿静脉血和脐带血的HBeAg含量与孕妇的HBeAg含量具有相关性(r=0.362,P=0.000;r=0.352,P=0.000),而静脉血和脐带血的HBsAg含量与孕妇血清的HBsAg含量无相关性(r=0.023,P=0.785;r=0.04,P=0.604).结论 慢性HBV感染孕妇所生新生儿脐带血和静脉血HBV标志物状态有良好的一致性,可以以脐带血的HBV标志物反映新生儿静脉血HBV标志物.     

Occurrence of Vertical Root Fractures after Apical Surgery: A Retrospective Analysis

IntroductionVertical root fractures (VRFs) are among the most frequent causes of tooth loss, mainly of endodontically treated teeth. However, very few data is available about the occurrence of VRFs following apical surgery.MethodsPatient charts from 864 patients with 1058 teeth treated with apical surgery (September 1999 to December 2018) were retrospectively evaluated, if a VRF had occurred after surgery. The following, possibly influencing factors were analyzed: sex and age, type of treated tooth, primary versus resurgery, technique of root-end preparation, and timepoint of VRF diagnosis. Endpoints were either tooth extraction or the last follow-up.ResultsThe study cohort (55% women, 45% men) had a mean age of 52.00 ± 13.97 years (range 9–93 years). The overall rate of VRFs after apical surgery was 4% (42 of 1058 teeth). Among these 42 teeth, 33.3% were mandibular first molars and 26.2% were maxillary second premolars. The most frequently affected root was the mesial root of mandibular first molars (28.6%). With regard to the study parameters, significant differences of VRF rates were observed only for the type of tooth treated.ConclusionsA low VRF rate of 4% was observed in this study. VRFs commonly occurred in maxillary premolars and mandibular molars, with the mesial root of mandibular first molars affected most frequently. This is in line with previous reports about VRFs in endodontically treated teeth without additional apical surgery.     

Diagnosis of Vertical Root Fractures by Cone-beam Computed Tomography in Root-filled Teeth with Confirmation by Direct Visualization: A Systematic Review and Meta-Analysis

IntroductionThe purpose of this review was to determine the diagnostic accuracy of cone-beam computed tomographic (CBCT) imaging in detecting vertical root fractures (VRFs) in root-filled teeth compared with a reference standard (direct visualization).MethodsElectronic searches were performed in Medline, Scopus, Cochrane, and gray literature for English language articles until June 2020. Prospective and retrospective clinical studies using CBCT imaging to diagnose VRFs in root-filled teeth were included. Case reports and in vitro studies were excluded. The Quality Assessment of Diagnostic Accuracy Studies 2 tool was used to assess the risk of bias and applicability concerns. Meta-analysis was performed using Stata 16.1 software (StataCorp, College Station, TX) via the MIDAS v.3.0 package and METANDI module. Publication bias was evaluated using Deeks’ funnel plot analysis. Grading of Recommendations, Assessment, Development, and Evaluations (GRADE) was performed to evaluate the certainty of evidence. This systematic review was registered in the Open Science Framework (10.17605/OSF.IO/7JKE2).ResultsEight articles were included in this systematic review and meta-analysis. Risk of bias assessment showed that 5 articles in the patient selection domain had low risk of bias with low applicability concern. In the index test and reference standard domains, 7 articles had moderate risk of bias with moderate applicability concern. Three articles had high RB in the flow and timing domain. There was no publication bias. CBCT imaging had a pooled sensitivity and specificity of 0.78 (95% confidence interval [CI], 0.64–0.88) and 0.80 (95% CI, 0.63–0.91), respectively, and an accuracy of 0.86 (95% CI, 0.83–0.89). CBCT imaging also had pooled positive and negative likelihood ratios of 4 and 0.2, respectively. In GRADE analysis, the quality of evidence was low for sensitivity and moderate for specificity when CBCT imaging was used for the diagnosis of VRF.ConclusionsThe overall quality assessment of the included articles showed that in the patient selection domain, the risk of bias was low, and it was moderate in the index test and reference standard domains. Evidence from this systematic review and meta-analysis indicates that CBCT imaging is still not a good tool for diagnosing VRFs in root-filled teeth compared with direct visualization.     

矢状向颌骨关系对全口重建颌位关系及排牙指导的初步研究

目的 分析Ⅰ、Ⅱ、Ⅲ类颌骨关系患者面部软、硬组织标志点的差异,为咬合重建或无牙颌患者修复治疗提供参考。方法 收集2019年1月—2020年9月至同济大学附属口腔医院就诊拍摄头颅定位侧位片的患者,共计329例。根据ANB大小分为Ⅰ类（2°~5°）、Ⅱ类（＞5°）和Ⅲ类患者（＜2°）;根据下颌平面角（FH MP）大小分为高角型（＞32°）、均角型（22°~32°）和低角型（＜22°）。对其进行面部软、硬组织高度、角度及比例的头影测量分析。结果 ①总人群中Ⅰ、Ⅱ、Ⅲ类患者共有22个测量值显示出统计学差异（P<0.05）,其中前下面高（ANS Me）满足Ⅲ类患者＞Ⅰ类患者＞Ⅱ类患者。②在高角型、均角型和低角型中,Ⅰ、Ⅱ、Ⅲ类患者仍有11个测量值具有统计学差异（P<0.05）。③相关性分析显示ANS Me与其余测量值均具有相关性,其中患者关节点到髁顶点的距离（Ar Gn）与ANS Me的相关系数|r|Ⅰ=0.839,|r|Ⅱ=0.732,|r|Ⅲ=0.806,均具有高度相关性。结论 Ⅰ、Ⅱ、Ⅲ类颌骨关系患者的面部测量值具有差异性,修复治疗时应该把颌骨关系作为考量之一。     

基于锥形束CT对邻面接触区釉质厚度的测量与分析

目的    应用锥形束CT（cone beam computed tomography,CBCT）测量上下颌牙列邻面接触区釉质厚度及邻接点距离牙合面和釉牙骨质界的垂直高度,为临床邻面去釉提供参考数据。方法    选取2019年6—10月于吉林大学口腔医院医学影像科行CBCT检查患者50例,根据纳入标准选取符合测量条件的牙齿145颗,运用Invivo软件测量每颗牙齿邻面接触区的釉质厚度及邻接点距离牙合面和釉牙骨质界的垂直高度。结果    从切牙至第一磨牙邻面接触区釉质厚度有逐渐增大的趋势;大多数牙位的远中邻面接触区釉质厚度大于近中（P < 0.05）,仅有上颌第一前磨牙和下颌第一磨牙的近远中邻面接触区釉质厚度差异无统计学意义（P > 0.05）;上下颌左右侧同名牙邻面接触区釉质厚度差异无统计学意义（P > 0.05）;除上颌中切牙-侧切牙、上下颌侧切牙-尖牙邻面接触区外,其余相邻牙齿近远中邻面接触区釉质厚度相似,差异均无统计学意义（均P > 0.05）;上颌邻面接触区平均釉质厚度大于下颌,差异有统计学意义（P < 0.05）;上下颌近远中邻接点到牙合面的垂直距离均小于到釉牙骨质界的垂直距离（均P < 0.05）。结论    不同牙位近远中邻面接触区釉质厚度不同,邻接点距离牙合面和釉牙骨质界的垂直高度也不同,临床邻面去釉时不同牙位去釉量不能按照同一标准进行,应结合CBCT影像资料制定个体化治疗方案。