Oral senile amyloidosis in senescence accelerated mouse (SAM)

Oral senile amyloidosis in senescence accelerated mouse (SAM) was examined for two SAM sublines (P/2/Iw and R/1/Iw) and for various ages by light microscopy, immunohistochemistry, and electron microscopy. The amyloid deposition, identified by green birefrigence following Congo red stain, was observed only in P/2/Iw. In P/2/Iw, no amyloid deposition was found at age 6 months; however, frequency and extent of such deposits increased with advancing age. Distribution of amyloid deposition was as follows: along papillary layers of mucous epithelium in the tongue, the gingiva, the palate, and the buccal mucosa; foci in connective tissues; along vessels, muscles, and minor salivary glands. Immunohistochemically, the amyloid deposition was positive with anti-ASSAM serum being raised against a unique amyloid protein ASSAM which probably induced "senile systemic amyloidosis". P/2/Iw is useful as an animal model of oral senile amyloidosis.     

10年早产儿及新生儿结果分析

目的 探讨早产儿的发生因素和对母儿的影响及防治。方法 收集１９８９年１月至１９９８年１２月,１０年中我院住院分娩的全部早产病例８１６例,按产５年和后５年分为两组进行回顾性分析。结果 前组早产数为５３４例,早产发生率为３．２０％;后组早产数为２８２例,发生率为３．０５％,两组发生率对比无统计学差异（Ｐ〉０．０５）。其流行病学调查显示在职业、流动人口数和产前检查方面两组对比有极其显著性差异（Ｐ〈０．０     

利用多种指标对哈尔滨市人群生命质量的评价

采用多种指标综合分析 ,对哈尔滨市区的人群生命质量进行了评价     

阴道分泌物胎儿纤维连接蛋白与宫颈长度联合预测早产的多中心前瞻性研究

Objective To discuss the clinical value of fetal fibronectin (fFN) and cervical length in predicting preterm birth. Methods Totally, 511 pregnant women at 22-35 weeks of gestations were enrolled from Dec. 2006 to Dec. 2007 at 4 tertiary hospitals in Beijing, and all with either symptoms or high risk factors of preterm birth, but without preterm premature rupture of membrane or vaginal bleeding. Cervical fFN were determined and cervical length of each subjects were measured by ultrasound. The pregnancy outcomes were compared. Results The sensitivity, specificity and negative predictive value (NPV) of fFN in predicting preterm birth within 7 d were 77.4%, 69.4% and 97. 9%, respectively; and 70.5%, 70.0% and 96.2% for deliveries within 34 weeks. The above figures changed to 54.8%, 66.3%, 95.8% for deliveries within 7 d and 59.1%, 67.2%, 94.6% for those within 34 weeks, respectively, when cervical length ≤3 cm was applied to predict preterm birth. When fFN and cervical length were combined, the sensitivity, specificity and NPV were 51.6%, 87.1%, 96.5% for deliveries within 7 d, and 45.5%, 87.60/00, 94.5% for those within 34 weeks, respectively. Conclusions FFN has higher NPV and specificity than cervical length in predicting preterm birth within 7 d and 34 weeks, and the positive predictive value and specificity can be improved when combined with cervical length.     

不同月龄大鼠血清IL-2、IL-4、IL-6含量比较

目的：比较不同月龄大鼠免疫功能及抗氧化能力的差异。方法：选择15、25月龄健康雄性SD大鼠各10只，以3月龄青年鼠10只为对照组。采用放免法测定血清白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、白细胞介素-6(IL-6)含量，并计算大鼠胸腺体重指数；采用化学法检测血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活力，丙二醛(MDA)含量。结果：衰老大鼠胸腺体重指数及IL-2、IL-4明显下降，IL-6水平升高SOD、GSH-PX活力下降，MDA含量增高，并且变化与月龄有关。结论：不同月龄大鼠衰老状况各有特点，不同年龄阶段应采用不同方法延缓衰老。     

补肾宁心益经丸治疗卵巢早衰30例临床观察

目的 观察补肾宁心益经丸治疗卵巢早衰(premature ovarian Failure,POF)的临床疗效.方法 选择60例肾虚型POF患者,随机分为两组各30例.治疗组口服补肾宁心益经丸治疗,对照组口服六味地黄丸,6个月为1个疗程.观察两组治疗前后临床症状、血清卵泡刺激素(FSH)及雌二醇(E2)水平的变化.结果 两组月经情况均有显著改善(P<0.01);治疗组血清FSH明显降低、E2明显升高,较治疗前有显著性差异(P<0.01).治疗组总有效率为70%(21例),对照组为40%(12例),两组比较有显著性差异(P<0.01).结论 补肾宁心益经丸能明显改善POF患者的临床症状和血清内分泌水平.     

早产分娩的流行病学研究

目的 :探讨早产分娩的部分流行病学特征。方法 :回顾性病例对照研究。结果 :与足月单胎母亲相比 ,单胎早产母亲 <2 0岁的比例显著提高 ,经产妇、产检次数不足、流动人口比例高 ;剖腹产仍是早产分娩较常见的分娩方式 ,且产后出血率和新生儿窒息率显著高于对照组。结论 :胎膜早破和医学指征的早产比例接近 ,均为 37% ,自然的早产占 2 2 % ;单胎早产分娩有季节性 ,月分布集中在 6月份     

牛磺酸治疗早产儿全静脉营养胆汁淤积的疗效

目的　观察早产儿应用全静脉营养后是否出现胆汁淤积 ,并探讨牛磺酸治疗胆汁淤积的效果。方法　将小于 33周的早产儿分为甲乙两组 ,均行全静脉营养 ,甲组应用牛磺酸治疗 ,乙组未应用牛磺酸治疗 ,并与大于 33周早产儿及足月儿对照 ,入院后第 1、7、14、2 1天采血测定丙氨酸转氨酶、直接胆红素、γ谷氨酰转肽酶。结果　入院后第 14、2 1天甲乙两组直接胆红素、γ谷氨酰转肽酶显著高于大于 33周的早产儿及足月儿 ,各时段甲乙两组 3项指标无显著差异。结论　较长时间全静脉营养可致胆汁淤积 ,牛磺酸对胆汁淤积治疗无明显效果 ,表明全静脉营养导致胆汁淤积原因的复杂性     

阴道毛滴虫长寿保障基因Tv-LAG1的克隆和表达

目的克隆原生动物阴道毛滴虫(Trichomonasvaginalis,Tv)长寿保障基因[longevityassurancegene1(LAG1)]的同源基因Tv-LAG1。构建Tv-LAG1原核表达重组体及表达其融合蛋白。方法从阴道毛滴虫cDNA表达文库中筛选LAG1的同源基因,用pET41表达载体与Tv-LAG1cDNA克隆构建原核表达重组体并表达融合蛋白,纯化表达产物用SDS-PAGE法鉴定。用纯化的融合蛋白免疫家兔,获得抗血清,采用Western-blot法对抗体特异性进行分析鉴定。结果成功构建pET41/Tv-LAG1原核表达重组体,并表达出预期大小的重组蛋白。用融合蛋白免疫家兔获得的抗体能特异性识别Tv-Lag1p/GST融合蛋白。结论Tv-Lag1p/GST融合蛋白获得高效表达,并成功纯化,初步实验证明具有一定的免疫原性,为研究Tv-Lag1p在模式生物阴道毛滴虫的细胞定位以及其功能奠定了基础。     

早产218例临床分析

目的探讨早产的危险因素、对母婴的影响及防治。方法对218例早产病例资料进行回顾性分析。结果胎膜早破、多胎妊娠、妊高征是引起早产的主要因素,不同孕周的早产对母亲影响无显著差别,对新生儿死亡,新生儿呼吸窘迫综合征(RDS)及低体重儿发生率有显著差异(P<0.01)。结论早产是新生儿发病和死亡的主要原因。胎龄越小、新生儿体重越轻其死亡率越高。防治早产是降低围产儿死亡率和患病率的关键。