The effect of collagen,Interleukin-l α and glicosaminoglycan on the neovascularization of central nervous system

Abstract

Neovascularization, the development of a new microvasculature, has an important role in physiological and pathological processes. The vascular changes in the brain can be easily detected because the proliferation of endothelial cells in its vascular structure is quite small, and so constitutes a good model for neovascularization studies. In the present investigation, to induce intracerebral neovascularization, we implanted collagen, Interleukin-l alpha (IL-lα) and glicosaminoglycan into the brain ofpigs, in order to test the hypothesis that IL-l α, collagen and glicosaminoglycan play a pivotal. role in the process of neovascularization. Both pure collagen and collagen combined with IL-l a induced neovascularization according to light-electron microscopic findings and values of enzymes' activities. In particular, collagen combined with IL-lα synergically affected the increase of neovascularization. However, glicosarrinoglycan did not affect it significantly. Although the results of this study provided us with some interesting data indicating the beneficial effects of collagen combined with IL-1α on neovascularization, further studies should be done to study the short term effect of these biochemical substances. [Neural Res 1998; 20: 513-525]     

Up-regulation of leptin in adipocytes exposed to high glucose and its effect in peritoneal angiogenesis

Objective By simulating a high-glucose condition of peritoneal dialysis (PD) fluid, to explore the effect of high glucose on the expression of leptin and its relationship with peritoneal angiogenesis. Methods Adipocytes differentiated from 3T3-L1 were divided into high glucose group (139 mmol/l glucose) and high mannitol group. Leptin levels in supernatant collected at 0 h, 12 h, 24 h and 48 h were measured by ELISA. Endothelial cells (ECs) were respectively cultured with normal glouse, high glucose, high mannitol condition, supernatants of adipocyte induced by normal glouse, high glucose and high mannitol, high glucose supernatants+leptin antibody, and high mannitol supernatants+leptin antibody. Tubular structure formation and migration of ECs were detected. Results Adipocytes exposed to high glucose for 48 h produced more leptin as compared with control group, high mannitol group, 12 h-high glucose group and 24 h-high glucose group (all P＜0.05). Compared with ECs in normal group, ECs in high glucose had less tubular structure formation and increased migration (all P＜0.01). Compared with those of ECs in high glucose, the tubular structure formation and the migration of ECs in adipocyte supernatants induced by high glucose had increased (all P＜0.01), and these effects were reduced by leptin antibody (all P＜0.01). Conclusion There is an up-regulation of leptin in adipocytes exposed to high glucose, which may be an alternative way to prevent peritoneal angiogenesis.     

超声造影评价动脉粥样硬化斑块内炎性反应与新生血管研究进展

动脉粥样硬化是各种心脑血管疾病的主要病理基础,斑块的稳定性与心脑血管事件密切相关,炎性反应和新生血管是易损性斑块的重要特征。采用CEUS评价斑块稳定性已成为可能。本文综述CEUS间接或直接检测斑块炎性反应和新生血管评估斑块稳定性的研究进展。     

预构皮瓣的研究与应用进展

Prefabricated flap is so named as the skin flap is prepared by prefabricating a circulation-rich skin flap by implanting a named blood vessel or a portion of fascia which is incorporated with rich bloo...     

血管内皮生长因子靶向抗肿瘤药物的研究与开发

血管内皮生长因子（VEGF）家族在肿瘤血管生成与转移中起关键作用,从不同途径阻断VEGF的生物学作用可以达到治疗肿瘤的目的。目前以VEGF及其受体为靶标研究开发了抗体、可溶性受体、酪氨酸激酶抑制剂、核酸和多肽类药物,均取得了较好的进展。     

2-甲氧雌二醇的抗乳腺癌作用及其机制

2-甲氧雌二醇（2-ME）是雌二醇的一种天然代谢产物,具有包括抗乳腺癌在内的广谱抗肿瘤作用。研究发现,这种抗肿瘤作用主要与其抑制肿瘤细胞增殖、诱导肿瘤细胞凋亡及抑制血管生成有关,而与雌激素受体无关。因此被认为可以用于乳腺癌治疗。2-ME因可口服、不良反应轻、与其他抗癌药物有协同作用而无交叉耐药,具有良好的应用前景。     

子宫内膜癌组织中内皮抑素的表达及与血管形成的关系

目的:探讨内皮抑素(Endostatin)在子宫内膜样腺癌组织中的表达及其与肿瘤血管形成的关系.方法:应用免疫组织化学方法(MaxVision法)检测66例子宫内膜样腺癌组织及26例增生期子宫内膜组织中内皮抑素的表达及CD34标记的MVD.结果:内皮抑素和MVD在子宫内膜样腺癌组织中高表达(P<0.01),与子宫内膜样腺癌分期有关(P<0.05).结论:内皮抑素检测可作为评价子宫内膜样腺癌生物学参数的参考指标之一.     

血管生成素-2基因及其受体Tie2在人肝癌组织的表达

目的探讨血管生成素-2（Angiopoietin-2）基因及受体Tie2在肝癌血管生成中的作用。方法原位杂交、免疫组织化学检测Angiopoietin-2基因、CD34、Tie2受体在22例人肝细胞性肝癌（HCC）、8例门静脉性肝硬化（CL）和8例正常肝（L）的表达,并分析Angiopoietin-2、Tie2表达水平与肿瘤生物学行为的相关性。结果（1）CD34在正常肝脏无表达,在CL偶见表达,而在HCC的表达明显增加(P＜0.01);（2）Tie2受体在CL偶见表达（11.3±8.7)/HP,而在HCC微血管内皮大量表达（52.4±16.7)/HP(P＜0.01);Tie2表达水平和肝癌直径、血管生成、门静脉侵犯等相关。（3）Angiopoietin-2基因在对照肝无表达,在CL少量表达(11.2±9.7)/HP,在HCC强阳性表达（36.4±17.5)/HP,表达水平和肿瘤血管生成、血管侵犯、组织学分级相关。结论人肝细胞性肝癌血管生成增加,Angiopoietin-2/Tie2和肝癌血管生成相关,可能参与肝癌血管生成的调控。     

血管生成抑制剂Rg3对胃癌生长和转移抑制作用的实验研究

目的：研究血管生成抑制剂Rg3对胃癌生长和转移的抑制作用。方法：完整组织块SCID鼠胃壁原位种植,建立类似于临床的胃癌转移模型。移植后第7天开始灌胃给药,Rg3剂量为0、2．5,5．0,10．0mg/kg,每日1次,共6周,移植后第8周末处死动物,测定原位肿瘤重量,观察转移情况,并采用抗CD31抗体的标记链霉亲和素－生物素免疫组化方法检测肿瘤内微血管密度。结果：Rg3对原位肿瘤的生长和转移均有抑制作用,2．5,5．0,10．0mg/kg剂量组的抑瘤率分别为52．3％,63．3％和71．6％,肝转移抑制率分别为37．8％,68．9％和84．4％,腹膜转移抑制率分别为36．4％,74．6％和74．6％,Rg3治疗后,肿瘤内微血管密度明显降低,结论：Rg3对胃癌的生长和转移有明显抑制作用。     

血管铸型方法检测组织工程骨修复羊胫骨缺损的远期血管化

目的采用血管铸型方法对组织工程技术修复中国青山羊胫骨大段骨缺损的远期血管化情况进行检测。方法中国青山羊3只，制备单侧胫骨20mm的骨与骨膜缺损，缺损部位内植入组织工程骨-珊瑚羟基磷灰石／骨髓基质干细胞（coral hydroxyapatite／bone marrow stem cells，CHAP／BMSCs）。术后3年分别行双侧后肢股动脉甲基丙烯酸甲酯血管铸型灌注，灌注后采用大体解剖、未脱钙骨片、扫描电镜等方法检测组织工程骨的远期血管化，并与健侧相同部位正常骨对照，观察其血管化效果。结果血管灌注后大体解剖观察示组织工程骨血管来源于周围软组织、髓腔血管及两端正常骨皮质。灌注后未脱钙骨磨片显示组织工程骨内微血管沿哈弗管和伏克曼管分布交织形成网状。采用图像分析仪比较组织工程骨与正常骨横切面磨片上血管相对面积，分别为（763．89±47．38）U／10万U与（788．00±65．83）U／10万U，差异无统计学意义（t=0．390，P〉0．05）。结论采用CHAP／BMSCs修复山羊胫骨大段骨缺损远期观察结果表明，新生骨远期血管化效果与正常骨无异，为组织工程骨下一步的临床应用提供可靠的依据。