安妥明对高胆固醇血症大鼠血脂、LCAT及过氧化酯质的影响

本实验探讨安妥明对实验性高胆固醇血症大鼠脂质代谢紊乱的影响。实验结果表明安妥明除具有已知的降低血清T—C、LDL—C和VLDL—C作用外,还可明显升高高胆固醇血症大鼠的血清HDL—C水平和LCAT活性,同时能明显降低血清及肝脏IPO的形成。进一步观察表明,安妥明对高密度脂蛋白亚组份的作用是使HDL_2-C升高,而对HDL_3-C无明显影响。     

Mechanism of adrenocortical toxicity induced by quinocetone and its bidesoxy-quinocetone metabolite in porcine adrenocortical cells in vitro

Quinocetone (QCT) is a new feeding antibacterial agent in the QdNOs family. The mechanism of its adrenal toxicity is far from clear. This study was conducted to estimate the adrenal cell damage induced by QCT and its bidesoxy-quinocetone (B-QCT) metabolite and to further investigate their mechanisms. Following doses of QCT increasing from 5 to 50 μM, cell apoptosis and necrosis, mitochondrial dysfunction and redox imbalance were observed in porcine adrenocortical cells. The mRNA levels of the six components of intermediary enzymes and the adrenal renin-angiotensin-aldosterone system (RAAS) displayed a dysregulation induced by QCT, indicating that QCT might influence aldosterone secretion not only through the upstream of the production but also through the downstream of the adrenal RAAS pathway. In contrast, B-QCT had few toxic effects on the cell apoptosis, mitochondrial dysfunction and redox imbalance. Moreover, LCMS-IT-TOF analysis showed that no desoxy metabolites of QCT were found in either cell lysate or supernatant samples. In conclusion, we reported on the cytotoxicity in porcine adrenocortical cells exposed to QCT via oxidative stress, which raised awareness that its toxic effects resulted from N→O groups, and its toxic mechanism might involve the interference of the steroid hormone biosynthesis pathway.     

脑复康对中老年认知功能和LPO影响的观察

选择认知功能减退患者２１例连服脑复康２个月，经治疗前后韦氏记忆量表（ＷＭＳ）和血过氧化脂质（ＬＰＯ）检测显示：使ＷＭＳ１０项目中的６项及量表总分、记忆商值均显著提高（Ｐ＜０．０１或＜０．０５），表明本药有改善记忆障碍的作用；使ＬＰＯ明显下降（Ｐ＜０．０５），对防治老年疾病的发生和延缓机体衰老具有一定意义。     

Protective effects of melatonin against spinal cord injury induced oxidative damage in rat kidney: A morphological and biochemical study

Spinal cord injury (SCI) induced oxidative stress affects multiple organ systems including the kidney. We studied the possible protective effects of melatonin on SCI-induced oxidative damage in renal tissues of rats. Wistar albino rats (n = 24) were exposed to SCI and divided into vehicle- or melatonin-treated SCI groups. Melatonin was administred intraperitoneally at a dose of 10 mg/kg for seven days. Renal tissues were investigated by light and electron microscopy. Furthermore, tissue malondialdehyde (MDA) and glutathione (GSH) levels and myeloperoxidase (MPO) and superoxide dismutase (SOD) activities were also determined. In the vehicle-treated SCI group, the renal histology was disturbed compared to controls, whereas the melatonin-treated SCI group showed significantly reduced degeneration of renal tissue as seen by both light and electron microscopy. MDA levels, MPO and SOD activities were increased and GSH levels were decreased in the vehicle-treated SCI group compared to controls. On the other hand, decreased MDA levels and MPO activities and increased GSH levels were observed in the melatonin-treated SCI group compared to vehicle-treated SCI group. These results showed that experimentally induced SCI caused oxidative stress in the rat kidney, whereas melatonin treatment reduced oxidative stress, suggesting that it may be used as a complementary therapy of renal problems occurring following SCI.     

胃得安胶囊联合兰索拉唑治疗胃溃疡的临床研究

目的探讨胃得安胶囊联合兰索拉唑治疗胃溃疡的临床效果。方法选取2016年7月—2018年7月天津市第五中心医院收治的胃溃疡患者96例,随机分成对照组(48例)和治疗组(48例)。对照组早餐前30 min口服兰索拉唑肠溶胶囊,30 mg/次,1次/d。治疗组在对照组基础上口服胃得安胶囊,2粒/次,3次/d。两组患者均连续治疗6周。观察两组患者临床疗效,同时比较治疗前后两组患者上腹痛评分、反酸评分、前列腺素(PG)E2、C反应蛋白(CRP)、白介素-17(IL-17)、超氧化物歧化酶(SOD)和脂质过氧化物(LPO)水平以及幽门螺杆菌根除率、溃疡愈合率及复发率。结果治疗后,对照组临床有效率为87.5%,显著低于治疗组的100.0%,两组比较差异有统计学意义(P0.05)。治疗后,两组患者中上腹痛评分和反酸评分均显著降低(P0.05),且治疗组患者中上腹痛评分和反酸评分明显低于对照组(P0.05)。治疗后,两组患者胃液中PGE2浓度及血清SOD水平均显著升高(P0.05),血清CRP、IL-17、LPO浓度则均显著降低(P0.05),且治疗组患者这些指标水平明显好于对照组(P0.05)。治疗后,治疗组幽门螺杆菌根除率、溃疡愈合率分别为93.8%、75.0%,均显著高于对照组(P0.05)。随访6个月,治疗组复发率为8.3%,比对照组的25%明显显著降低(P0.05)。结论胃得安胶囊联合兰索拉唑治疗胃溃疡的整体疗效切实,促进溃疡愈合,增强胃黏膜的防御功能及机体的抗氧化能力。     

CT引导下不同方法注射臭氧对腰椎间盘突出患者SOD、LPO影响

目的比较不同方法注射臭氧（0,）治疗腰椎间盘突出（LDH）对患者血液中超氧化物歧化酶（SOD）、脂质过氧化物（LPO）影响。方法120例LDH患者随机分成2组,每组60例。观察组男性41例,女性19例;年龄23～59岁,平均年龄44．5岁;对照组男性38例,女性22例;年龄21—62岁,平均年龄45．7岁。正常组15例健康体检者．其中男性9例,女性6例;年龄25～55岁,平均年龄43．6岁。观察组采用弯套针旋转多向注射法注射0,至病变椎间盘内进行治疗,对照组采用常规方法注射0,治疗。于6个月后统计疗效,并测定患者治疗前后血浆SOD、LP0水平。正常组只做SOD、LP0检测。结果观察组痊愈显效率85．0％,对照组68．3％,两组间疗效差异有统计学意义（X^2=4．66,P〈0．05）;治疗前观察组和对照组比较SOD、LPO各项数值差异均无统计学意义（P〉0．05）,但这两组SOD活性及SOD／LPO值明显低于正常组,LP0明显高于正常组（P〈0．01）。治疗后2组SOD活性及SOD／LPO值均有明显升高且趋于正常,LP0含量均有明显下降且趋于正常,与治疗前比较差异有显著统计学意义（P〈0．001）,但观察组变化显著并更接近正常值,与对照组比较差异有统计学意义（P〈0．05和O．01）,而与正常组比较差异无统计学意义（P〉0．05）。结论0,治疗LDH能明显提高SOD活性,抑制体内脂质过氧化反应,具有显著改善自由基代谢紊乱作用,而运用弯套针旋转多向注射比常规注射作用更显著,具有更好的效果。     

飞行人员疗养前后血浆尿液过氧化脂质测定

目的测定飞行人员疗养前后血浆、尿液过氧化脂质（ＬＰＯ）含量，旨在评定疗养效果。方法随机抽取２０例飞行人员，以入院疗养两天内作为疗养前，疗养至２０天时间为疗养后，测定疗养前后血浆、尿液ＬＰＯ含量。结果结果发现疗养后比疗养前血浆ＬＰＯ下降、尿液ＬＰＯ含量升高（Ｐ＜０．０５），疗养后血浆ＬＰＯ下降和尿液ＬＰＯ升高负相关（ｒ＝－０．６３８，Ｐ＜０．０５）。结论血浆、尿液ＬＰＯ可以作为疗养效果评定的客观指标。     

长航对女性黄褐斑的影响研究

目的分析长航对女性黄褐斑的影响。方法选取参加长航任务的17例女性黄褐斑患者(黄褐斑组),并设对照组15例健康女性(健康组),检测长航前、后症状及血清促黑素细胞激素、过氧化脂质、超氧化物歧化酶水平的变化。结果长航3个月后黄褐斑患者皮损面积和颜色较长航前均不同程度扩大和加深(P<0.05),伴随症状失眠、便秘、月经不调均不同程度加重(P<0.05)。黄褐斑组和健康组长航3个月后血清促黑素细胞激素水平均较前升高(P<0.05),黄褐斑组血清促黑素细胞激素长航前、后较健康组均高(P<0.05);黄褐斑组长航3个月后过氧化脂质水平升高、超氧化物歧化酶水平降低(P<0.05),而健康组长航前、后差异无统计学意义(P>0.05)。结论长航能使黄褐斑皮损扩大、颜色加深,伴随症状加重,并使黄褐斑患者的血清促黑素细胞激素和过氧化脂质水平升高、超氧化物歧化酶水平降低。     

唾液腺提取物抗衰老作用初步研究

目的：研究唾液腺提取物（SGE）的抗衰老作用。方法：以400mg/kg和1500mg/kg剂量的SGE分别饲喂SD大鼠75d，以400mg/kg剂量的SGE饲喂HIN小鼠45d，采用NBT光化还原法和TBA荧光法分别测定其红细胞SOD活性及血清LPO含量，同时测定胸腺和脾指数。结果：SGE均能显著提高大、小鼠血细胞SOD活性和大鼠SOD比活性，显著降低大、小鼠血清LPO含量，提高大鼠胸腺指数，结论：SGE能显著提高动物红细胞SOD活性，降低血清LPO含量，提高胸腺指数，具有明显的抗衰老作用。     

固本保元浸膏敷脐对大鼠溃疡性结肠炎氧自由基及其清除作用的影响

目的:研究固本保元浸膏对溃疡性结肠炎治疗作用.方法:应用大鼠溃疡性结肠炎的模型,给予固本保元浸膏敷脐,观察固本保元浸膏(GE)对大鼠血液和组织中氧自由基清除能力.结果:研究表明,GE能提高血液和组织中SOD及GSH-PX活性,降低LPO的含量,与病理组比较有显著差异(p＜0.01,P＜0.001).结论:固本保元浸膏具有抗自由基及其清除的能力.