全文获取类型
收费全文 | 4542篇 |
免费 | 354篇 |
国内免费 | 89篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 59篇 |
妇产科学 | 369篇 |
基础医学 | 341篇 |
口腔科学 | 39篇 |
临床医学 | 533篇 |
内科学 | 339篇 |
皮肤病学 | 23篇 |
神经病学 | 138篇 |
特种医学 | 416篇 |
外国民族医学 | 3篇 |
外科学 | 260篇 |
综合类 | 970篇 |
预防医学 | 225篇 |
眼科学 | 22篇 |
药学 | 409篇 |
1篇 | |
中国医学 | 79篇 |
肿瘤学 | 753篇 |
出版年
2023年 | 40篇 |
2022年 | 81篇 |
2021年 | 157篇 |
2020年 | 124篇 |
2019年 | 140篇 |
2018年 | 128篇 |
2017年 | 171篇 |
2016年 | 180篇 |
2015年 | 177篇 |
2014年 | 295篇 |
2013年 | 252篇 |
2012年 | 293篇 |
2011年 | 352篇 |
2010年 | 312篇 |
2009年 | 278篇 |
2008年 | 230篇 |
2007年 | 252篇 |
2006年 | 224篇 |
2005年 | 170篇 |
2004年 | 135篇 |
2003年 | 115篇 |
2002年 | 108篇 |
2001年 | 65篇 |
2000年 | 70篇 |
1999年 | 39篇 |
1998年 | 36篇 |
1997年 | 44篇 |
1996年 | 43篇 |
1995年 | 28篇 |
1994年 | 35篇 |
1993年 | 32篇 |
1992年 | 27篇 |
1991年 | 23篇 |
1990年 | 23篇 |
1989年 | 19篇 |
1988年 | 26篇 |
1987年 | 18篇 |
1986年 | 18篇 |
1985年 | 32篇 |
1984年 | 24篇 |
1983年 | 12篇 |
1982年 | 20篇 |
1981年 | 15篇 |
1980年 | 19篇 |
1979年 | 14篇 |
1978年 | 16篇 |
1977年 | 11篇 |
1976年 | 12篇 |
1974年 | 15篇 |
1973年 | 13篇 |
排序方式: 共有4985条查询结果,搜索用时 15 毫秒
141.
CA125检测在活动性结核判定及疗效评估中的价值 总被引:12,自引:1,他引:12
目的 探讨血清CA12 5测定在活动性肺结核判定及疗效评估中的价值。方法 应用化学发光法分别对3 0例活动性肺结核治疗前、治疗后 2个月、4个月、6个月以及 3 0例非活动性肺结核和 3 0例健康体检者血清CA12 5进行测定。并对结果进行分析。结果 活动性肺结核治疗前血清CA12 5水平显著高于非活动性肺结核及健康体检者 (P <0 0 1)。活动性肺结核治疗前CA12 5水平显著高于治疗后各期测定值 (P <0 0 1) ,且治疗后血清CA12 5水平呈递减趋势。活动性肺结核治疗后 6个月血清CA12 5水平与非活动性肺结核比较无明显差异 ( P >0 0 5 )。结论 血清CA12 5测定可作为判定活动性肺结核及评估疗效的可靠指标 相似文献
142.
Cor triatriatum: study of 20 cases. 总被引:7,自引:0,他引:7
Twenty cases of cor triatriatum are reported. The diagnosis was confirmed by necropsy in 16 cases and at the time of operation in 4. The lesion occurred as an isolated anomaly in 7 cases; in 13, other associated cardiac anomalies were present. Three anatomic types of cor triatriatum were identified in the cases studied at necropsy: diaphragmatic (10 cases), hourglass (3) and tubular (3). The diaphragmatic type was also present in all four cases in which the diagnosis was confirmed at operation. Associated anomalies were found in five cases of the diaphragmatic type and in each case of the hourglass of tubular types. In isolated cor triatriatum the clinical findings were characteristic of pulmonary venous and arterial hypertension. In two cases, one with a communication between the right atrium and the accessory left atrial chamber and one with partial anomalous pulmonary venous connection associated with cor triatriatum, the clinical findings suggested a large left to right shunt with pulmonary arterial hypertension. The clinical findings varied in the cases with associated anomalies, and it was difficult to determine the cause of disturbance of the circulation. 相似文献
143.
邓涛 《中国现代药物应用》2020,(6):1-3
目的分析不同肝病患者以血清癌胚抗原(CEA)、糖链抗原125(CA125)、糖链抗原199(CA199)、甲胎蛋白(AFP)联合检测的诊断价值。方法选取299例肝病患者作为观察组,另选取3605例接受体检的健康者作为对照组。两组研究对象均接受血清CEA、CA125、CA199、AFP检测。对比两组研究对象血清CEA、CA125、CA199、AFP水平,同时对比观察组不同分级和不同类型肝硬化患者的CEA、CA125、CA199、AFP水平。结果观察组中肝炎患者CEA、CA125、CA199、AFP水平分别为(3.85±0.14)ng/ml、(34.59±11.04)U/ml、(25.22±1.31)U/ml、(10.33±4.24)ng/ml;肝硬化患者CEA、CA125、CA199、AFP水平分别为(4.11±0.18)ng/ml、(253.29±12.21)U/ml、(28.79±1.52)U/ml、(37.41±4.03)ng/ml;肝癌患者CEA、CA125、CA199、AFP水平分别为(6.93±0.17)ng/ml、(287.37±11.94)U/ml、(93.32±1.77)U/ml、(3859.47±120.44)ng/ml。对照组研究对象CEA、CA125、CA199、AFP水平分别为(3.05±0.12)ng/ml、(11.38±5.21)U/ml、(18.48±1.01)U/ml、(3.01±1.00)ng/ml。观察组患者CEA、CA125、CA199、AFP水平均明显高于对照组,差异有统计学意义(P<0.05)。Ⅲ级肝硬化患者血清CEA(7.66±0.21)ng/ml、CA125(385.45±13.25)U/ml、CA199(24.77±2.05)U/ml、AFP(46.31±5.02)ng/ml均明显高于Ⅱ级患者的(4.42±0.23)ng/ml、(262.41±11.06)U/ml、(18.31±2.31)U/ml、(25.94±4.52)ng/ml和Ⅰ级患者的(3.54±0.25)ng/ml、(178.49±11.04)U/ml、(8.24±2.47)U/ml、(17.38±4.49)ng/ml,差异有统计学意义(P<0.05)。胆汁性肝硬化患者CEA水平为(5.89±1.05)ng/ml高于酒精性肝硬化患者的(5.22±1.03)ng/ml和病毒性肝硬化患者的(3.49±1.02)ng/ml,差异有统计学意义(P<0.05);酒精性肝硬化患者CA125、CA199水平分别为(312.59±13.23)、(26.34±2.12)U/ml高于胆汁性肝硬化患者的(128.59±21.05)、(18.31±2.01)U/ml和病毒性肝硬化者的(214.01±13.22)、(19.30±2.05)U/ml,差异有统计学意义(P<0.05);病毒性肝硬化患者AFP水平为(57.39±11.05)ng/ml高于胆汁性肝硬化患者的(6.33±1.04)ng/ml和酒精性肝硬化患者的(5.35±1.09)ng/ml,差异有统计学意义(P<0.05)。结论不同肝病患者以血清CEA、CA125、CA199、AFP联合检测能够鉴别肝病类型,准确性较高,值得应用推广。 相似文献
144.
Yin and Yang are two complementary forces that together describe the nature of real-world elements. Yin is the dark side; Yang is the light side. We describe microRNAs having both Yin and Yang characteristics because they can contribute to normal function (Yang) but also to autoimmunity, myeloproliferation, and cancer (Yin). We have been working on a number of microRNAs that have these dual characteristics and here we focus on two, miR-125b and miR-146a. We have concentrated on these two RNAs because we have very extensive knowledge of them, much of it from our laboratory, and also because they provide a strong contrast: the effects of overexpression of miR-125b are rapid, suggesting that it acts directly, whereas the effects of miR-146a are slow to develop, suggesting that they arise from chronic alterations in cellular behavior. 相似文献
145.
M.T. Pinto L.D.F. Nicolete E.S. Rodrigues P.V.B. Palma M.D. Orellana S. Kashima D.T. Covas 《Brazilian journal of medical and biological research》2013,46(8):676-680
Multipotent mesenchymal stromal cells (MSCs) were first isolated from bone marrow and
then from various adult tissues including placenta, cord blood, deciduous teeth, and
amniotic fluid. MSCs are defined or characterized by their ability to adhere to
plastic, to express specific surface antigens, and to differentiate into osteogenic,
chondrogenic, adipogenic, and myogenic lineages. Although the molecular mechanisms
that control MSC proliferation and differentiation are not well understood, the
involvement of microRNAs has been reported. In the present study, we investigated the
role of miR-125b during osteoblastic differentiation in humans. We found that
miR-125b increased during osteoblastic differentiation, as well as Runx2 and ALPL
genes. To study whether the gain or loss of miR-125b function influenced osteoblastic
differentiation, we transfected MSCs with pre-miR-125b or anti-miR-125b and cultured
the transfected cells in an osteoblastic differentiation medium. After transfection,
no change was observed in osteoblastic differentiation, and Runx2, OPN, and ALPL gene
expression were not changed. These results suggest that the gain or loss of miR-125b
function does not influence levels of Runx2, OPN, and ALPL during osteoblastic
differentiation. 相似文献
146.
《Journal of biomaterials science. Polymer edition》2013,24(10):1123-1146
A tri-block-coupling polymer of stearyl poly(ethylene oxide)-4,4′-methylene diphenyl diisocyanate-stearyl poly(ethylene oxide)(MSPEO), was used as a surface modifying additive (SMA) and the MSPEO-modified poly(ether urethane) (PEU) surfaces were prepared by the process of dipcoating. The surface analysis by XPS revealed the surface enrichment of poly(ethylene oxide) (PEO). On the coating-modified surfaces, the bovine serum albumin (BSA) adsorption, respectively, from the low and high BSA bulk concentration solutions was correspondingly characterized by the methods of radioactive 125I-probe and ATR-FTIR. The bovine serum fibrinogen (Fg)-adsorption from the Fg bulk solution and the BSA-Fg competing adsorption from the BSA-Fg binary solutions were also characterized by radioactive 125I-probe. The reversible BSA-selective in situ adsorption on MSPEO-modified PEU surfaces were achieved, and the performance of blood compatibility on the coating-modified surfaces was also confirmed, respectively, by plasma recalcification time (PRT) and prothrombin time (PT) tests. 相似文献
147.
148.
PurposeMultiple pathways are involved in inducing liver fibrosis, which can damage the integrity of liver. Among them, miR-125b has been found to exert an activating action on hepatic stellate cells. Endoplasmic reticulum stress and autophagy lead to liver disorders. Here, we evaluated the therapeutic influence of miR-125b on the endoplasmic reticulum function in injured livers submitted to bile duct ligation.Materials and MethodsFor inducing injury, bile duct ligation was done on miR-125b transgenic rats (miR-125b-Tg) in wild type rats. The rat T-6 cells received transfection of miR-125b mimic and Tunicamycin. Protein expressions were observed by western blot analysis.ResultsCompared to wild type rats, liver-injured rats showed significant impairment of liver function as assessed by the total bilirubin levels. The miR-125b-Tg rats showed decrease in activity of aspartate transaminase and alanine transaminase. Liver tissues of miR-125b-Tg rats showed weaker fibrotic matrix formation. Upregulation of miR-125b decreased the bile duct ligation-mediated hepatic disturbances for the expressions of endoplasmic reticulum kinase, inositol-requiring kinase 1alpha, sXBP1, CHOP, LC3, p62, ULK, and caspase-3/-8/-9. T-6 cells transfected with miR-125b mimic and treated with Tunicamycin caused decrease in levels of cleaved caspase-3, sXBP1, CHOP, and LC3. The miR-125b signaling showed protective effect on the liver tissues subjected to injury and fibrosis histopathology.ConclusionThis study demonstrates a novel insight into the miR125b-mediated stabilization of endoplasmic reticulum integrity, which slows the progression of injury-induced hepatic deterioration. 相似文献
149.
150.
Yu-Jie Huang Zhi-Fei Cao Jie Wang Jian Yang Yi-Jun Wei Yu-Chen Tang Yin-Xiang Cheng Jian Zhou Zi-Xiang Zhang 《World Journal of Clinical Cases》2021,9(17):4143-4158
BACKGROUND MUC16, encoding cancer antigen 125, is a frequently mutated gene in gastric cancer. In addition, MUC16 mutations seem to result in a better prognosis in gastric cancer. However, the mechanisms that lead to a better prognosis by MUC16 mutations have not yet been clarified.AIMTo delve deeper into the underlying mechanisms that explain why MUC16 mutations signal a better prognosis in gastric cancer.METHODSWe used multi-omics data, including mRNA, simple nucleotide variation, copy number variation and methylation data from The Cancer Genome Atlas, to explore the relationship between MUC16 mutations and prognosis. Cox regression and random survival forest algorithms were applied to search for hub genes. Gene set enrichment analysis was used to elucidate the molecular mechanisms. Single-sample gene set enrichment analysis and “EpiDISH” were used to assess immune cells infiltration, and “ESTIMATE” for analysis of the tumor microenvironment.RESULTSOur study found that compared to the wild-type group, the mutation group had a better prognosis. Additional analysis indicated that the MUC16 mutations appear to activate the DNA repair and p53 pathways to act as an anti-tumor agent. We also identified a key gene, NPY1R (neuropeptide Y receptor Y1), which was significantly more highly expressed in the MUC16 mutations group than in the MUC16 wild-type group. The high expression of NPY1R predicted a poorer prognosis, which was also confirmed in a separate Gene Expression Omnibus cohort. Further susceptibility analysis revealed that NPY1R might be a potential drug target for gastric cancer. Furthermore, in the analysis of the tumor microenvironment, we found that immune cells in the mutation group exhibited higher anti-tumor effects. In addition, the tumor mutation burden and cancer stem cells index were also higher in the mutation group than in the wild-type group.CONCLUSIONWe speculated that the MUC16 mutations might activate the p53 pathway and DNA repair pathway: alternatively, the tumor microenvironment may be involved. 相似文献