次声90 dB和130 dB致大鼠肝细胞损伤的超微结构改变

目的：探讨相同频率(16 Hz)不同声压水平(90 dB与130 dB)次声作用下大鼠肝细胞超微结构损伤特点及意义．方法：将雄性SD大鼠66只，随机分为对照组(6只)、实验1(90 dB)和实验2(130 dB)组．根据次声作用时间，实验1，2组各分为1，7，14，21和28 d时间组，每组6只．在相同频率不同声压水平次声环境下每日暴露1次，每次2 h；取肝左右叶两个部位组织用戊二醛和锇酸固定，透射电镜下观察肝超微结构变化．结果：1 d组：90 dB环境作用下肝超微结构无明显损伤变化，130 dB环境作用下肝细胞内有脂滴出现；7，14和21 d组不同环境和天数次声作用后肝损伤出现脂滴逐渐增多和肝细胞部分变性坏死改变，发现肝细胞内的各种结构随次声作用次数增多其损伤程度逐渐加重；28 d组其损伤程度均有一定程度恢复．结论：次声下可以对肝细胞超微结构造成不同程度损伤，28 d后肝细胞对次声的损伤作用存在着一定适应现象．     

穴位注药治疗精神分裂症的研究

目的　观察穴位注射丹参合并小剂量抗精神病药物与单用抗精神病药物治疗精神分裂症的疗效。方法16 0例患者随机分为治疗组 10 0例 ,对照组 6 0例 ;辨证分为 5型 ,用阴阳经相配取穴的方法 ,根据疾病的虚实 ,用迎随和徐疾补泻手法 ,治疗组每日注射 1次 ,10次为 1疗程 ,共治疗 3个疗程 ;用简明精神症状量表 (BPRS)和药物副反应量表 (TESS)评定疗效和副反应 ,治疗结束后随访两年。结果　治疗组痊愈率、显效率与对照组间差异有显著性意义 (P <0 0 5 )。BPRS和TESS量表于每疗程结束后两组评分比较差异均有显著性意义 (P <0 0 5 ) ;复发率与对照组间差异有显著性意义 (P <0 0 5 )。结论　穴位注射丹参具有见效快、副作用少、远期疗效好的特点。实施有效的护理 ,适时的健康教育是提高患者依从性 ,保证有效治疗、取得良好效果的重要手段。     

细胞移植用于心肌修复--细胞心肌成形术的研究进展

由于心肌细胞的增殖能力很低，细胞移植作为一种新的治疗方法用于改善心功能及心肌活力已受到广泛的关注。目前已有胚胎干细胞、骨髓间充质干细胞和内皮干细胞在体外诱导分化为心肌细胞；动物实验中用于心肌移植的细胞有胚胎心肌细胞、造血干细胞、骨髓间充质干细胞、骨骼肌成肌细胞、内皮干细胞、肝干细胞和神经干细胞。其中成肌细胞移植用来改善心肌梗死后的心脏功能，已有临床报道，并取得成功。     

硒抑制镍诱导的人肺成纤维细胞的DNA损伤

目的　探讨三氧化二镍(Ni2O3)对人肺成纤维细胞(human lung fibroblasts,HLF)的DNA损伤作用以及Na2SeO3的保护作用。方法　应用单细胞凝胶电泳(single cell gel electrophoresis,SCGE)检测DNA损伤。结果　Ni2O3 处理HLF细胞4 h,SCGE检测出DNA损伤程度高于对照组,差异有显著性(P<0.01)。而Ni2O3 Na2SeO3 处理组DNA损伤程度低于Ni2O3 组,差异有显著性(P<0.01)。结论　硒可抑制镍诱导的人肺成纤维细胞的DNA损伤。     

强噪声引起豚鼠听器损伤的实验研究

本文将豚鼠暴露于120dBSPL强噪声2小时后第2、10和20天,分别行听性脑干反应(ABR)和光镜电镜内耳结构观察。结果声暴露后ABR反应闽与正常动物差异十分明显(P<0.01)。光镜电镜观察发现实验组动物内耳血管纹毛细血管淤血,细胞变性。外毛细胞听毛及表皮板明显受损。说明急性噪声性损伤后内耳微循环障碍,所引起听器缺氧性损伤的形态学特点。     

Effect of isoelectric point on biodistribution and inflammation imaging with indium-111-labelled IgG

Electrostatic effects play an important role in protein interactions and may alter the biodistribution of antibodies. To study the effect of molecular charge on the biodistribution and infection imaging properties of human polyclonal immunoglobulin G (IgG), its iso electric point was varied by changing the level of diethylene triamine penta-acetic acid (DTPA) substitution: 0.8, 0.9, 3.7, 5.1 and 5.9 DTPA/IgG. Biodistributions of the different IgG preparations were determined at 10 min, 1, 6, 24, and 48 h post injection in normal rats, and infection imaging properties were determined in rats withEscherichia coli thigh infections. The biodistribution was significantly affected by pl. The immunoglobulin preparations with 0.9 and 3.7 DTPA/IgG showed faster clearance from the circulation and generally lower accumulation in most organs. The images had a target-to-background ratio of approximately 1.3–2.3:1. These results suggest that even though targeting is not affected by the level of DTPA substitutions, preparations with 0.9 and 3.7 DTPA/IgG may be superior imaging agents because of reduced accumulation by background organs.     

Neuroprotection with felbamate: a 7- and 28-day study in transient forebrain ischemia in gerbils

The use of glutamate antagonists and GABA agonists may protect neurons from the effects of transient ischemia. Felbamate is a new antiepileptic drug with glutamate antagonist and GABA agonist properties, We tested the efficacy of felbamate in a gerbil model of transient forebrain ischemia. Damage assessment was done with silver staining at 7 and 28 days after 5 min of bilateral carotid occlusion, Cerebral cortex, hippocampus (CA1 and CA4), thalamus and striatum were evaluated on a 4-point scoring system, The animals sacrificed at 28 days were also tested in a water-maze task to assess recovery of function, The initial dose of felbamate (300 mg/kg) was given 30 min before the ischemic insult in one set of animals and 30 min after the insult in another set of animals. There were 8 animals tested per group (total: 48 animals). There was significant neuronal protection with the use of felbamate, both before and after ischemia in all regions of the brain. Protection was seen in animals sacrificed at 7 and 28 days, Protection was moderate when felbamate was used before ischemia. It was highly significant when felbamate was given 30 min after the insult. Behavioral studies however did not show any difference in the felbamate treated animals versus the saline treated controls. The structural protection with felbamate was very significant when used in the post-ischemic period. This window for protection merits further evaluation in relation to the clinical setting of stroke.     

Effects of Phentolamine on Hemorrheology and Hemodynamics in Dogs with Acute Liver Damage

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Suppression of Gl Arrest and Enhancement of G2 Arrest by Inhibitors of Poly(ADP-ribose) Polymerase: Possible Involvement of Poly(ADP-ribosyl)ation in Cell Cycle Arrest Following γ-Irradiation

Low-dose γ-irradiation of mouse embryonic fibroblast C3D2F1 3T3-a cells caused Gl arrest along with G2 arrest and inhibition of replicative DNA synthesis. When the cells were cultured in the presence of inhibitors of poly(ADP-ribose) polymerase [EC 2.4.2.30], such as 3-aminobenzamide, benzamide and luminol, Gl arrest of C3D2F1 3T3-a cells was suppressed and enhancement of G2 arrest was observed. In contrast, 3-aminobenzoic acid, a non-inhibitory analog of 3-aminobenzamide, did not suppress Gl arrest following γ-irradiation. These results suggest that the poly(ADP-ribosyDation reaction is critical for the pathway of Gl arrest and is also involved in the pathway of G2 arrest.     

百草枯所致大鼠弥漫性肺损伤的病理特点

作者选用ＳＤ大鼠进行腹腔内一次性注入百草枯２５ｍｇ／ｋｇ后，在７个不同时间点观察其肺病理组织和超微结构改变。结果显示：百草枯可引起弥漫性毛细血管内皮细胞和肺泡１型上皮细胞为主的整层气一血屏障结构的损害，主要表现为肺泡水肿、出血、透明膜形成和（或）程度不等的肺泡炎，当损伤不能完全修复时则局部终结为纤维化。