全文获取类型
收费全文 | 3627篇 |
免费 | 263篇 |
国内免费 | 72篇 |
专业分类
耳鼻咽喉 | 21篇 |
儿科学 | 90篇 |
妇产科学 | 37篇 |
基础医学 | 390篇 |
口腔科学 | 33篇 |
临床医学 | 166篇 |
内科学 | 420篇 |
皮肤病学 | 26篇 |
神经病学 | 258篇 |
特种医学 | 71篇 |
外国民族医学 | 1篇 |
外科学 | 77篇 |
综合类 | 252篇 |
现状与发展 | 1篇 |
预防医学 | 1336篇 |
眼科学 | 19篇 |
药学 | 536篇 |
中国医学 | 77篇 |
肿瘤学 | 151篇 |
出版年
2023年 | 66篇 |
2022年 | 115篇 |
2021年 | 165篇 |
2020年 | 150篇 |
2019年 | 155篇 |
2018年 | 150篇 |
2017年 | 147篇 |
2016年 | 138篇 |
2015年 | 134篇 |
2014年 | 226篇 |
2013年 | 278篇 |
2012年 | 187篇 |
2011年 | 246篇 |
2010年 | 162篇 |
2009年 | 188篇 |
2008年 | 180篇 |
2007年 | 153篇 |
2006年 | 146篇 |
2005年 | 124篇 |
2004年 | 104篇 |
2003年 | 91篇 |
2002年 | 52篇 |
2001年 | 45篇 |
2000年 | 52篇 |
1999年 | 33篇 |
1998年 | 25篇 |
1997年 | 32篇 |
1996年 | 22篇 |
1995年 | 23篇 |
1994年 | 19篇 |
1993年 | 21篇 |
1992年 | 15篇 |
1991年 | 18篇 |
1990年 | 11篇 |
1989年 | 13篇 |
1988年 | 10篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 27篇 |
1984年 | 32篇 |
1983年 | 21篇 |
1982年 | 30篇 |
1981年 | 30篇 |
1980年 | 22篇 |
1979年 | 14篇 |
1978年 | 13篇 |
1977年 | 15篇 |
1976年 | 9篇 |
1975年 | 9篇 |
1974年 | 11篇 |
排序方式: 共有3962条查询结果,搜索用时 783 毫秒
991.
Alexander Hoischen Peer Arts Nienke Wieskamp Walter van der Vliet Sascha Vermeer Marloes Steehouwer Petra de Vries Rowdy Meijer Jorge Seiqueros Nine V.A.M. Knoers Michael F. Buckley Hans Scheffer Joris A. Veltman 《Human mutation》2010,31(4):494-499
Massively parallel sequencing has tremendous diagnostic potential but requires enriched templates for sequencing. Here we report the validation of an array‐based sequence capture method in genetically heterogeneous disorders. The model disorder selected was AR ataxia, using five subjects with known mutations and two unaffected controls. The genomic sequences of seven disease genes, together with two control loci were targeted on a 2‐Mb sequence‐capture array. After enrichment, the patients' DNA samples were analyzed using one‐quarter Roche GS FLX Titanium sequencing run, resulting in an average of 65 Mb of sequence data per patient. This was sufficient for an average 25‐fold coverage/base in all targeted regions. Enrichment showed high specificity; on average, 80% of uniquely mapped reads were on target. Importantly, this approach enabled automated detection of deletions and hetero‐ and homozygous point mutations for 6/7 mutant alleles, and greater than 99% accuracy for known SNP variants. Our results also clearly show reduced coverage for sequences in repeat‐rich regions, which significantly impacts the reliable detection of genomic variants. Based on these findings we recommend a minimal coverage of 15‐fold for diagnostic implementation of this technology. We conclude that massive parallel sequencing of enriched samples enables personalized diagnosis of heterogeneous genetic disorders and qualifies for rapid diagnostic implementation. Hum Mutat 31:1–8, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
992.
Birney MH Hardie TL Crowley EM 《Journal of the American Academy of Nurse Practitioners》2006,18(5):221-227
PURPOSE: To examine the association between reported absences and parental smoking in school-age children, aged 6-17, and to specifically explore the impact of maternal smoking on the health and attendance of school-age children. DATA SOURCES: The sample of 7488 parent-child dyads was randomly selected from the 2002 National Health Interview Survey, a multipurpose cross-sectional household interview survey conducted by the Centers for Disease Control and Prevention. The parent sample included 2673 men and 4375 women. Children's ages ranged from 6 to 17 with a mean of 11.7 years. CONCLUSIONS: Maternal, but not paternal, present and past smoking behavior significantly impacts the child's wellness and school attendance. The reasons for this disparity are unclear but may relate to synergistic effects of pre- and postnatal nicotine exposure, the traditional role of mother as caregiver, or specific smoking habits that increase environmental tobacco exposure. IMPLICATIONS FOR PRACTICE: Assessment and educational strategies for families regarding the hazards of childhood exposure to environmental tobacco smoke are indicated. Specific implications for the role of the nurse practitioner across diverse specialties are addressed with emphasis on the need for development of gender, age, and culturally sensitive smoking cessation programs and support networks. 相似文献
993.
Genomic basis for stimulated respiration by plants growing under elevated carbon dioxide 总被引:1,自引:0,他引:1
994.
Aim: This prospective observational study investigated the effect of environmental tobacco smoke (ETS) on frequency and severity of common infantile infections.
Methods: In a representative sample of 926 infants, parental smoking was recorded at months 1 and 9 postpartum, and all infantile infectious episodes were recorded at 1, 3, 6, 9 and 12 months postpartum.
Results: Both parents were regular smokers all through the first year in 107 (11.6%), at least one smoked regularly or occasionally in 492 (53.1%), and parents did not smoke at all in 327 (35.3%) families. Among mothers, 168 (18.1%) smoked perinatally. Infantile ETS exposure was associated with increased frequency of total infectious episodes (p = 0.025) and hospitalizations for infection (p = 0.007). In ETS exposed infants, birth in autumn and presence of siblings contributed to increased frequency of most infections and of hospital admissions for infection. By contrast, exclusive breastfeeding protected against the effect of ETS on total infantile infections (OR 0.982, 95% CI 0.965–0.999; p = 0.036), hospital admissions for infection (OR 0.980, 95% CI 0.961–0.999; p = 0.036) and thrush (OR 0.973, 95% CI 0.951–0.996; p = 0.022).
Conclusion: Our findings point to harmful effect of ETS on infantile health and further suggest that this effect may be enhanced or diminished by other factors. ETS should be regarded as a preventable risk factor for infections in infancy. 相似文献
Methods: In a representative sample of 926 infants, parental smoking was recorded at months 1 and 9 postpartum, and all infantile infectious episodes were recorded at 1, 3, 6, 9 and 12 months postpartum.
Results: Both parents were regular smokers all through the first year in 107 (11.6%), at least one smoked regularly or occasionally in 492 (53.1%), and parents did not smoke at all in 327 (35.3%) families. Among mothers, 168 (18.1%) smoked perinatally. Infantile ETS exposure was associated with increased frequency of total infectious episodes (p = 0.025) and hospitalizations for infection (p = 0.007). In ETS exposed infants, birth in autumn and presence of siblings contributed to increased frequency of most infections and of hospital admissions for infection. By contrast, exclusive breastfeeding protected against the effect of ETS on total infantile infections (OR 0.982, 95% CI 0.965–0.999; p = 0.036), hospital admissions for infection (OR 0.980, 95% CI 0.961–0.999; p = 0.036) and thrush (OR 0.973, 95% CI 0.951–0.996; p = 0.022).
Conclusion: Our findings point to harmful effect of ETS on infantile health and further suggest that this effect may be enhanced or diminished by other factors. ETS should be regarded as a preventable risk factor for infections in infancy. 相似文献
995.
Mélissa M. Moreno Christiane Linster Olga Escanilla Jo?lle Sacquet Anne Didier Nathalie Mandairon 《Proceedings of the National Academy of Sciences of the United States of America》2009,106(42):17980-17985
Perceptual learning is required for olfactory function to adapt appropriately to changing odor environments. We here show that newborn neurons in the olfactory bulb are not only involved in, but necessary for, olfactory perceptual learning. First, the discrimination of perceptually similar odorants improves in mice after repeated exposure to the odorants. Second, this improved discrimination is accompanied by an elevated survival rate of newborn inhibitory neurons, preferentially involved in processing of the learned odor, within the olfactory bulb. Finally, blocking neurogenesis before and during the odorant exposure period prevents this learned improvement in discrimination. Olfactory perceptual learning is thus mediated by the reinforcement of functional inhibition in the olfactory bulb by adult neurogenesis. 相似文献
996.
目的 了解环境刺激对未成熟脑可塑性的影响及其在脑损伤后功能康复中的意义。 方法 选用雄性SD大鼠制作新生大鼠缺氧缺血性脑损伤(HIBD)模型,采用早期触摸和丰富环境刺激进行干预,观察大脑感觉运动皮层及海马CA3区突触超微结构参数的变化。结果 电镜下观察到2组大鼠两脑区均存在GRAY1型(即兴奋型)突触和穿孔突触。皮质部位干预组与非干预组比较,突触界面曲率和突触后致密物厚度(PSD)均增大,穿孔突触的百分比较高。海马部位干预组突触界面曲率和PSD厚度均较非干预组增大。结论 环境刺激对HIBD大鼠功能的促进作用可能与突触超微结构参数的改变,增强突触可塑性有关。 相似文献
997.
Christopher M.A Brett Denise A Fungaro José M Morgado M.Helena Gil 《Journal of electroanalytical chemistry (Lausanne, Switzerland)》1999,468(1):26-33
Various polymer coatings have been investigated for the protection of mercury thin-film electrodes in the square wave anodic stripping voltammetry of environmental samples using batch injection analysis, with injection of untreated samples of volume 50 μl directly over the sensing electrode. Polymer coatings studied include those with controlled porosity, and cation-exchange polymers based on sulphonated polymers. Of the polymers tested, films of ca. 1 μm thickness made from Nafion® mixed with 5% poly(vinyl sulphonic acid) were found to give the best results in tests with the model surfactants Triton-X-100 detergent, sodium dodecyl sulphate and protein standard. The validity of the approach is demonstrated by application to real samples. 相似文献
998.
999.
1000.
Some virus species can resist harsh environmental conditions, surviving on surfaces for long periods with the possibility of being transmitted to susceptible hosts. Studies are limited on the efficacy of disinfectants against viruses dried onto surfaces, in particular, with the identification of new pathogenic non-enveloped viruses that are expected to have high resistance to disinfection, such as parvoviruses. In this study a range of commonly used biocides, including heat, was tested against porcine parvovirus (PPV), minute virus of mice (a parvovirus), poliovirus type 1, adenovirus type 5, and vaccinia virus dried onto surfaces. PPV was the most resistant species identified, since many biocides generally considered as effective against non-enveloped viruses and used for high level disinfection demonstrated limited activity. Ethanol had poor activity against all non-enveloped viruses. Effectiveness against these viruses may be important in preventing nosocomial transmission of emerging pathogenic species such as bocavirus and other parvoviruses. This work confirms the need to validate disinfection products against viruses dried onto surfaces and demonstrates that PPV is a particularly resistant surrogate. 相似文献