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91.
秋水仙碱对肿瘤坏死因子—α基因表达的影响 总被引:6,自引:0,他引:6
微管聚合抑制剂秋水仙碱(Col,10μmol/L)可抑制LPS刺激PU5-1.8细胞的TNF-a基因表达,以前工作曾证实Col主要抑制TNF-αmRNA的转录。本研究进一步证实这种抑制作用呈延迟性,即Col需作用3小时才能充分表现其负性影响。而且这种抑制作用可被蛋白合成抑制剂放线菌酮(CHX)完全阻断。单用Col对LPS诱导的TNF-αmRNA稳定性无明显影响,而与CHX联用时则可明显延长LPS诱导的TNF-αmRNA半衰期。以上资料堤示,Col对TNF-α基因表达的影响可能是对其在转录及转录后水平综合影响的结果。 相似文献
92.
秋水仙碱对肝纤维化大鼠肝脏基质金属蛋白酶—1及其抑制因 … 总被引:3,自引:0,他引:3
目的 观察秋水仙碱对纤维化肝脏基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响。从胶原降解的角度探讨秋水仙碱对肝纤维化有无逆转作用及可能存在的机制。方法 制备免疫性大鼠肝纤维化模型,并给予秋水仙碱治疗;通过RT-PCR检测MMP-1、TPMP-1的表达,并作Ⅰ、Ⅲ型胶原的免疫组化以及Masson胶原染色。结果 发现秋水仙碱对肝纤维化大鼠MMP-1的表达无明 相似文献
93.
秋水仙碱对肝纤维化大鼠肝脏基质金属蛋白酶-1及其抑制因子-1表达的影响 总被引:2,自引:0,他引:2
目的 观察秋水仙碱对纤维化肝脏基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响,从胶原降解的角度探讨秋水仙碱对肝纤维化有无逆转作用及可能存在的机制.方法 制备免疫性大鼠肝纤维化模型,并给予秋水仙碱治疗;通过RT-PCR检测MMP-1、TIMP-1的表达,并作Ⅰ、Ⅲ型胶原的免疫组化以及Masson胶原染色.结果 发现秋水仙碱对肝纤维化大鼠MMP-1的表达无明显影响(P>0.05),但可以抑制TIMP-1的表达(P<0.05),促进Ⅰ、Ⅲ型胶原的降解(P<0.05);然而在病理形态学的观察中,未发现秋水仙碱治疗组与肝纤维化模型组之间存在的显著性差异(P>0.05).结论 秋水仙碱可以抑制纤维化肝脏TIMP-1的表达,从而增强间质胶原酶的活性,促进Ⅰ、Ⅲ型胶原的降解,产生抗肝纤维化的作用,但其作用有限. 相似文献
94.
Frank Wehner Frank Mußhoff Martin M. Schulz David D. Martin Heinz-Dieter Wehner 《Forensic science, medicine, and pathology》2006,2(3):193-197
The accidental ingestion of plant material containing colchicines is a rare cause of lethal poisoning. The death of a married
couple who mistook colchicines for Bear's garlic (Allium ursinum) and prepared the latter as a salad is the tragic topic of this article. After discussing the specificity of the histological
findings, a chemicotoxicological method using liquid chromatographic mass spectrometric (LC-MS/MS) is presented. Toxicological
analyses using LC-MS/MS revealed colchicine concentrations between 36.6 ng/mL and 98.3 ng/mL in the heart blood and between
22.7 ng/mL and 78.4 ng/mL in the femoral blood of the victims. 相似文献
95.
Masaki Tokunaga Yoichi Ueta Toyohi Isse Yuko Hara Keiko Tanaka Shigeki Yamamoto Narutoshi Kabashima Izumi Shibuya Yukio Hattori Hiroshi Yamashita 《Brain research》1997,774(1-2)
The existence of PTH-related peptide (PTHrP) in the hypothalamus was examined by immunohistochemistry in colchicine-treated rats. Two days after intracerebroventricular administration of colchicine dense PTHrP-like immunoreactivity (LI) was observed in the external zone of the median emminence (ME). PTHrP-LI cells were found in the paraventricular nucleus, the supraoptic nucleus and the periventricular region of the third ventricule. The effects of PTHrP on intracellular Ca2+ concentrations ([Ca2+]i) were examined by a Ca2+ imaging method using fura-2 in perifused preparations of isolated rat anterior pituitary cells. The rise in [Ca2+]i induced by PTHrP was found in approximately 17% of the cells examined. These results suggest that PTHrP-LI cells in the hypothalamus may project to the ME and contribute to the anterior pituitary function. 相似文献
96.
Over a period of 7 years, among 175 boys under the age of 16 years with familial Mediterranean fever (FMF), 16 (9%) developed
28 episodes of scrotal swelling that was unilateral in 26 (93%) and bilateral in 2 (7%). Fever and pain were present in 15
(94%) children; fever was characterized by a gradual onset and pain was moderate in intensity. The episodes were self-limiting
and lasted from 8 h to 5 days. Scrotal swelling was the presenting feature of FMF in 4 (25%) patients. Six (38%) children
underwent surgery; the operative findings, available in 3, showed a normal testis and epididymis and inflammation of the tunica
vaginalis. The self-limiting nature of the episodes lasting for a few days was similar to the clinical course of serositis
seen in FMF. This strongly suggests that inflammation of the tunica vaginalis, resulting in scrotal swelling, is another feature
of FMF serositis. The gradual onset of fever, pain, swelling, and recurrence in a boy of Mediterranean origin, especially
in the presence of a relevant family history, strongly points toward the diagnosis of FMF and conservative management. Early
diagnosis and prophylactic colchicine therapy are expected to avert recurrences, which may result in ischemic testicular necrosis
and FMF nephropathy.
Accepted: 4 September 1998 相似文献
97.
秋水仙碱对成纤维细胞产生细胞因子以及分泌细胞外基质的影响 总被引:1,自引:0,他引:1
目的 阐明秋水仙碱对体外培养人肾脏成纤维细胞 (FB)产生炎症因子转化生长因子 β1(TGF β1)、白细胞介素 1β(IL 1β)以及细胞外基质的影响。 方法 体外培养人胚胎肾脏FB ,经不同浓度秋水仙碱 ( 5 0 μmol/L、10 0 μmol/L、2 0 0 μmol/L、4 0 0 μmol/L)预处理 1h后 ,加入含 10 0μg/ml脂多糖 (LPS)的FB完全培养液继续培养 18h ,以未经LPS刺激也无秋水仙碱预处理的FB细胞为对照。实验终点收集细胞和上清。分别利用RT PCR方法观察秋水仙碱对FB产生TGF β1和IL 1βmRNA表达的影响 ;ELISA法测定细胞上清TGF β1、IL 1β以及胶原COLⅢ、COLⅣ蛋白含量。结果 ( 1)单纯 10 0 μg/mlLPS刺激后 ,FB产生TGF β1和IL 1βmRNA分别较未经刺激的正常对照组上调 3倍 (RI值分别为 6 6 1± 1 6、2 2 3± 2 0 ,q =5 90 5 ,P =0 0 0 2 )和 4 7倍 (RI值分别为2 2 0± 2 2、4 7± 0 8,q =10 6 8,P =0 0 0 9)。细胞上清TGF β1和IL 1β蛋白含量均明显高于基础含量 [TGF β1分别为 :( 5 16± 14 )pg/ml和 ( 4 2 0± 5 ) pg/ml(q =80 3,P =0 0 12 ) ,IL 1β分别为 :( 3 4± 0 3) pg/ml和 ( 0 3± 0 1) pg/ml( q=2 97 9,P =0 0 0 3) ]。 ( 2 )秋水仙碱明显抑制FB之TGF β1mRNA以及蛋白表达 ,而 相似文献
98.
99.
Bakkaloglu A 《Pediatric nephrology (Berlin, Germany)》2003,18(9):853-859
Familial Mediterranean fever (FMF) is the most frequent periodic syndrome characterized by recurrent attacks of polyserositis. Fever, abdominal pain, chest pain, and arthritis/arthralgia are the leading symptoms. It is an autosomal recessive disorder, which primarily affects Jewish, Armenian, Turkish, and Arab populations. The FMF gene (MEFV) has recently been cloned to chromosome 16p, which encodes pyrin. Genotype-phenotype correlation is not well established. Amyloidosis is the most severe complication of FMF. The SAA1-/ genotype was associated with an increased risk of amyloidosis. Colchicine treatment not only decreases the frequency and severity of attacks, but also prevents amyloidosis. Certain vasculitides, namely Henoch-Schonlein purpura and polyarteritis nodosa, are more frequent among FMF patients. 相似文献
100.
Colchicine has been reported to be a safe and effective medication in the treatment of early phase of Peyronie's disease (PD). However here we reported two patients, presenting with PD during high dose colchicine treatment for familiar mediterranean fever (FMF). 相似文献