中西医结合治疗痛风的临床疗效观察

目的：观察中西医结合治疗痛风的临床疗效。方法：随机选取2005年7月～2008年3月我们院经治62例痛风患者,随机分为两组,治疗组32人,对照组30人。对照组患者口服西药秋水仙碱;治疗组在对照组的基础上同时口服中药,然后比较两组临床疗效。结果：治疗组在临床治愈率、改善血尿酸、缓解急性期症状等方面均明显好于对照组（P〈0．05）,而且治疗组复发率低,不良反应少。结论：采用中药联合西药治疗痛风的办法能够改善痛风症状,并且减少患者血尿酸水平和减少复发。     

Safety,Efficacy, and Drug Survival of Colchicine in Recurrent Aphthous Stomatitis in a Real-World Setting

BackgroundRecurrent aphthous stomatitis (RAS) is a common disorder characterized by episodic ulcerations in the oral mucosa. Although colchicine has been a common systemic treatment for RAS, there is still considerable uncertainty regarding its efficacy and drug survival in this setting.ObjectiveWe aimed to study drug survival, efficacy, and safety of colchicine for the treatment of RAS, especially in the real clinical setting.MethodsBetween 2012 and 2016, 150 patients given colchicine for RAS were selected for a single-centre retrospective study of real-world efficacy and drug survival.ResultsAmong the 114 patients who qualified, 81.6% showed moderate or substantial responses (>25% improvement). Gastrointestinal complications (16.7%), neutropenia (3.5%), and liver enzyme elevation (4.4%) were reported within 2 weeks after initiating treatment. Delayed adverse manifestations were rare. One year after onset, colchicine use was sustained in roughly one-half (49.5%) of patients, whereas many (30.3%) had discontinued the drug, primarily due to lack of efficacy or adverse events. In Cox proportional hazard analysis, minor ulcers were identified as potential determinants of longer drug survival owing to less probability of non-efficacy. However, major ulcers had emerged as predictors of early discontinuation due to lack of efficacy.ConclusionIn patients with RAS, colchicine may be an effective and safe treatment amenable to long-term maintenance. Monitoring of adverse events within 2 weeks after initiating treatment is advisable to ensure safe administration.     

HPLC法测定秋水仙碱片的含量

目的建立高效液相色谱法测定秋水仙碱片的含量。方法采用C18色谱柱(150mm×4.6mm,5μm),流动相为0.05mol/L磷酸二氢钾-甲醇(50:50),流速1.0mL/min,检测波长350nm。结果秋水仙碱在6.444～15.036μg/mL范围内,线性关系良好,回归方程:Y=0.0202X+0.2333,r=0.9997。平均回收率:100.3%(n=6)。结论本法简便、准确、专属性强。     

秋水仙碱对皮下移植G422小鼠生存期影响的实验研究

目的观察秋水仙碱对G422胶质瘤小鼠生存期的影响。方法建立小鼠皮下G422胶质瘤模型,将80只健康小鼠随机均分成4组,每组20只,其中3组为实验组,分别给予0.500 mg/（kg·d）、0.050 mg/（kg·d）、0.005 mg/（kg·d）的秋水仙碱治疗,另一组为对照组。分别在G422胶质瘤接种后的第2、5天,将0.1 ml药物注射于接种G422部位处,一共2次。用药结束后,观察小鼠平均生存期,并计算小鼠生存时间延长率。结果实验组的平均生存时间与对照组相比均明显延长,其生存时间分别为（44.28±1.07）d、（42.79±1.64）d、（40.09±1.39）d,生存时间延长率分别为57.14%、50.99%、42.86%;而对照组为（28.28±1.77）d;结论在瘤局部注射秋水仙碱可显著提高G422荷瘤鼠的生存期。     

Autotetraploidy induced in Nianmaohuangqin (Radix Scutellariae viscidulae) with colchicine in vitro

Objective

To establish and optimize the propagation of Nianmaohuangqin (Radix Scutellariae Viscidulae) and induce and characterize polyploidy of Nianmaohuangqin (Radix Scutellariae Viscidulae).

Methods

Buds from germinating seed-derived explants were induced by tissue culture. With an orthogonal test, different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to determine the optimal concentrations for the propagation of Nianmaohuangqin (Radix Scutellariae Viscidulae). The different concentrations of IAA and rooting powder (ABT) were used to induce rooting. A 0.3% w/v colchicine solution was used to induce polyploidy and the induced buds was identified by root-tip chromosome determination and stomatal apparatus observation.

Results

A large number of buds could be induced directly from epicotyl and hypocotyl explants on Murashige and Skoog (MS) (Murashige and Skoog 1962) medium supplemented with 1.1–1.3 mg/L BAP and 0.2 mg/L IAA. Root induction and development could be observed within 20 days of inoculation on 1/2 MS medium supplemented with 0.2 mg/L IAA and 0.1 mg/L ABT. Furthermore, 27 lines of autotetraploid individuals were obtained with a plantlet chromosome number of 2n=4x=36.

Conclusion

Autotetraploid lines could be obtained through induction with colchicine in vitro, proving that this method might be used for plant selection and breeding.     

痛风定胶囊联合西药治疗急性痛风性关节炎随机平行对照研究

[目的]观察痛风定胶囊联合西药治疗急性痛风性关节炎疗效。[方法]使用随机平行对照方法,将82例住院及门诊患者按随机数字表方法分为两组,对照组38例秋水仙碱首剂3mg,1～2h0．5mg,直至症状缓解或出现不良反应。24h内不可超过6mg,并在症状缓解后48h内不需服用,72h后0．5～1mg／片。别嘌呤醇0．1g／次,2～3／d。治疗组44例痛风定胶囊3～4粒／次,3次／d。西药治疗同对照组。连续治疗7d为1疗程。临床症状、关节功能、血尿酸检查、不良反应。治疗1疗程,判定疗效。[结果]治疗组痊愈30例,显效8例,有效4例,无效2例,总有效率95．45％。对照组痊愈20例,显效5例,有效6例,无效7例,总有效率81．58％。治疗组疗效优于对照组（P〈0．05）。[结论]痛风定胶囊联合西药治疗急性痛风性关节炎疗效满意,副作用低,值得推广。