Effect of 5-Aminoisoquinolinone on the Adhesion of Colon Carcinoma

Objective： 5-Aminoisoquinolinone, a water-soluble, potent inhibitor of the activity of poly （adenosine 5＇-diphosphate ribose） polymerase, plays an important role in the tissue injury associated with ischaemia-reperfusion injury and inflammation by inhibiting the activity of poly （adenosine 5＇-diphosphate ribose） polymerase and the expression of cell adhesion molecules such as ICAM-1, P-selectin et al. But how about it in the tumor is not clear. The aim of the present study was to study the effects of 5-Aminoisoquinolinon on the adhesion of colon carcinoma line HT-29 cells to human umbilical vein endothelial cells; and the effects of 5-Aminoisoquinolinon on the expression of ICAM-1, P-selectin and the activity of poly （adenosine 5＇-diphosphate ribose） polymerase in colon carcinoma HT-29 cells. Methods： The adhesion of HT-29 cells to human umbilical vein endothelial cells was detected by adhesive experiment. Immunocytochemically Streptavidin-Peroxidase method was used to investigate the expression of ICAM-1, P-selectin and Poly （adenosine 5＇-diphosphate ribose）（ the product of poly （adenosine 5＇-diphosphate ribose） polymerase activation）. Results： the results of the adhesion assay of HT-29 cells to HUVEC showed that the OD570 value in each 5-AIQ-treated group was significant lower than that in the control group （5-AIQ-untreated） in a dose-dependent manner. The expression of ICAM-1, P-selectin and Poly （adenosine 5＇-diphosphate ribose） was significant lower in 5-Aminoisoquinolinone-treated HT-29 cell group than that in 5-Aminoisoquinolinoneuntreated groups. Conclusion： The data suggest that 5-Aminoisoquinolinone can inhibit the adhesion of HT-29 cells to human umbilical vein endothelial cells. 5-Aminoisoquinolinone also can inhibit poly （adenosine 5＇-diphosphate ribose） polymerase activation and the expressions of ICAM-1 and P-selectin in HT-29 cells. 5-Aminoisoquinolinone probably contributes to the prevention of tumor cell metastasis. Further study is needed.     

妇幼保健院医疗设备配置的思考和探讨

妇幼保健院运营的模式,服务的对象都有别于综合性医院。通过对设备使用效益的调查、分析,提出妇幼保健院医疗设备的配置,应符合本院的特点。配置应遵循:适用性、特色性、经济性、先进性,不可盲目求新、求全,避免设备使用的低效益。     

人胎儿前庭器超微结构的观察

对人胎儿前庭器超微结构的研究,国内外报道甚少。本文对18至36周人胎儿颞骨10具进行了扫描电镜和透射电镜的观察,并对应用Surface preparation法制做人胎儿前庭器标本的方法进行了探讨。本文观察所见:豚鼠平衡斑的生理状态与人胎儿很近似,体面积比人胎儿平衡斑小,感觉毛长,细胞排列更整齐。28周以后的胎儿因前庭周围骨质变硬,制做标本时可以分别取出两囊斑和壶腹嵴。     

尿道损伤46例临床分析

本文报道尿道损伤46例,占同期泌尿系损伤的70%。分析尿道损伤手术术式和疗效,以尿道对端吻合最好.尿道会师牵引术亦有其优点。     

妊娠压力对大鼠子代早期空间学习记忆能力的影响

【目的】探索妊娠期精神压力对大鼠子代早期空间学习、记忆能力的影响。【方法】实验采取产前束缚压力,建立妊娠期大鼠精神压力暴露模型。然后对子代进行Morris水迷宫测试,分析妊娠期压力对子代早期学习和记忆的影响及性别间的差异,同时检测实验前、后子鼠的血清肾上腺酮的变化。【结果】①在隐蔽平台试验中,对照组的平均潜伏期较实验组短,但差异无显著性(F=0.599,P>0.05)。②在保持力试验中,对照组在原平台所在象限逗留的时间大于实验组,差异有显著性(F=4.588,P<0.05)。③子代血清肾上腺酮的测定结果表明:水迷宫测试前,实验组和对照组相比,差异无显著性(F=0.169,P>0.05);实验后,实验组血清肾上腺酮比对照组高,差异具有显著性(F=6.098,P<0.05)。【结论】①妊娠压力可导致子代学习,记忆能力的改变;②妊娠压力造成子代在应激情况下血清肾上腺酮的过度分泌。③妊娠压力对子代的影响没有明显性别差异。     

过程管理在医院消毒灭菌中的应用

目的将过程管理应用于医院消毒灭菌中，规范医院消毒灭菌工作。方法以医院感染管理相关法规为依据，对消毒灭菌流程进行调查诊断，找出流程中的关键环节，以关键环节为质控点，对工作流程进行改造并实施过程管理。结果改善了硬件设施，工作流程更为科学，提高了技术水平和工作效率，消毒灭菌合格率明显提高。结论以医院感染管理相关法规为依据，以流程中的关键环节为质控点的过程管理，有利于规范医院消毒灭菌工作，实现由经验管理、终末管理向依法管理、过程管理转变。     

高原地区部队医院谋求新发展应处理好七个关系

从高原部队医院实际出发,阐述了适应新形势谋求新发展应处理好的七个关系:即继承与创新的关系;坚持“姓军为兵的服务方向与对外有偿服务的关系;基础建设与人才建设的关系;日常医疗保障与科研的关系;发挥自身特色与学习借鉴的关系;硬件建设与软件建设的关系;科技研究与成果转化的关系。     

Nerve growth factor-induced increase in calcium uptake by PC12 cells

Treatment of PC12 cells with nerve growth factor (NGF) produces a rapid and transient increase in calcium uptake into the cells. The increased uptake is maximal after 5 minutes of NGF treatment, but after 15 minutes of NGF treatment, no such increase can be observed. The effect of NGF is partially inhibited by blockers of L-type calcium channels. K-252a, an alkaloid-like kinase inhibitor that usually is found to inhibit the actions of NGF on PC12 cells, produces an increase in calcium uptake similar to, but smaller than, that seen with NGF. NGF had no effect on calcium release under these conditions.     

The effects of peripherald-amphetamine, 4-OH amphetamine, and epinephrine on maintained discharge in the locus coeruleus with reference to the modulation of learning and memory by these substances

d-Amphetamine, 4-OH amphetamine, and epinephrine have been shown in many behavioral studies to facilitate memory when given post-training. The effect of these drugs on the maintained discharge of cells in the locus coeruleus (LC) was investigated using a route of administration (intraperitoneal) and a log-dose range of these drugs comparable to those used in the behavioral experiments.d-Amphetamine profoundly suppressed maintained discharge: an inhibitory effect was observed at every dose (0.1, 1.0, 10.0 mg/kg). In contrast, only the highest dose of 4-OH amphetamine (8.2 mg/kg) inhibited activity in the LC, and this effect was a modest one. Unlike the amphetamines, epinephrine (500 μ/kg) elevated maintained discharge. These results are discussed in the contex of the hypothesized involvement of the LC in the enhancement of memory by these drugs.     

基于无线网络及条形码技术的图书排架、定位系统

利用无线网络与条形码技术,建立起图书排架、定位系统,可实现图书排架和图书的准确定位.该系统可有效地解决传统管理方法易产生的乱架和死书问题,提高图书借阅效率并降低管理人员的工作强度.