鹅掌藤中三萜类化合物的分离与鉴定

目的研究五加科鹅掌柴属植物鹅掌藤(Schefflera arboricola)枝茎的化学成分.方法采用柱色谱分离,通过理化数据和光谱分析确定化合物的结构.结果从鹅掌藤乙酸乙酯提取物中分离得到7个三萜化合物,分别鉴定为羽扇醇(1)、桦木酸(2)、3-epi-betulinic acid(3)、齐墩果酸(4)、3-乙酰齐墩果酸(5)、mesembryanthemoidigenic acid(6)、quinatic acid(7).结论化合物1、5、6、7为首次从该属植物中分得.     

Pentamer peptide from Fas antigen ligand inhibits tumor‐growth with solid‐bound form found by peptide array

Abstract: Despite their low molecular weight and simple structure, peptides regulate vital reactions of various cells. With higher standards of safety and purity required in clinical researches, chemically synthesized peptides are considered as an ideal alternative material for animal‐derived materials in the regulation of cellular events. However, effective high‐throughput assay for studying peptide‐cell interactions has not been established to design peptide with objective function. Here, we report the effectiveness of the utilization of peptide array combined with cell assay for the design of cell‐interactive peptides. As a model case, peptide that regulates tumor cell viability with support‐bound form was explored. By culturing cells on peptide array, we found a novel 5‐mer sequence CNNLP (Cys‐Asp‐Asp‐Leu‐Pro) that strongly inhibits viability of tumor cells (leukemia and adenocarcinoma) from human Fas antigen ligand. We here indicate the advantageous features of peptide array, which are ‘feasibility in examining combinatorial amino acid substitutions’ and ‘indicative data consist of effective and ineffective substitutions from an assay’, contributes greatly for studying peptide‐cell interaction. These features differentiate peptide array from other peptide library screening strategy. As a result, we succeeded in obtaining 29 novel tumor‐growth inhibitory peptides with it solid‐bound form, and structural rules that suggest ideal peptide design for acquiring objective peptide function.     

杂交贝母生物碱成分的研究

目的 :对杂交贝母进行化学成分研究。方法 :采用IR ,¹H NMR ,¹³ C-NMR ,2D-NMR ,MS及与对照品和文献对照等方法鉴定结构。结果 :分离并鉴定了 7个化合物 :湖贝乙素 (hupehenirine ,ZF1) ;湖贝嗪 (hupehenizine ,ZF2 ) ;浙贝乙素 (peiminine ,verticinone ,ZF3) ;湖贝甲素 (hupehenine ,ZF4 )、异浙贝甲素 (isoverticinone ,ZF5 )、浙贝甲素(peimine ,verticine ,ZF6 )、湖贝甲素苷 (hupeheninoside,ZF7) ;结论 :化合物 1～ 7均为首次从杂交贝母中得到。     

Structural and functional biomarkers of the insula subregions predict sex differences in aggression subscales

Aggression is a common and complex social behavior that is associated with violence and mental diseases. Although sex differences were observed in aggression, the neural mechanism for the effect of sex on aggression behaviors remains unclear, especially in specific subscales of aggression. In this study, we investigated the effects of sex on aggression subscales, gray matter volume (GMV), and functional connectivity (FC) of each insula subregion as well as the correlation of aggression subscales with GMV and FC. This study found that sex significantly influenced (a) physical aggression, anger, and hostility; (b) the GMV of all insula subregions; and (c) the FC of the dorsal agranular insula (dIa), dorsal dysgranular insula (dId), and ventral dysgranular and granular insula (vId_vIg). Additionally, mediation analysis revealed that the GMV of bilateral dIa mediates the association between sex and physical aggression, and left dId–left medial orbital superior frontal gyrus FC mediates the relationship between sex and anger. These findings revealed the neural mechanism underlying the sex differences in aggression subscales and the important role of the insula in aggression differences between males and females. This finding could potentially explain sexual dimorphism in neuropsychiatric disorders and improve dysregulated aggressive behavior.     

以前胡为例,探讨中药活性成分研究的思路与方法

前胡为中国药典收载的常用中药，我们在对其化学成分和生物活性深入系统研究的过程中，逐渐形成了对中药活性成分研究思路和方法的认识。本文主要结合我们长期的研究工作，综述了1993年后中药前胡在化学成分、质量标准、资源开发、有效成分的结构修饰与全合成以及药理活性方面的研究近况，其主要目的是介绍如何以前胡活性成分研究为基础，全面提高中药前胡的研究水平，并为新药开发奠定基础。对前胡的研究已初步建立起了活性成分的现代化研究模式，期望其研究思路与方法能为其它中药活性成分的研究提供参考。     

淡黄香茶菜地下部位化学成分

目的: 研究淡黄香茶菜Isodon flavida地下部分的主要化学成分. 方法: 采用硅胶、葡聚糖凝胶Sephadex LH-20,MCI 树脂等多种柱色谱技术进行分离纯化,运用理化性质和现代波谱技术鉴定结构. 结果: 从淡黄香茶菜地下部分的70%丙酮提取物的乙酸乙酯部位分离鉴定11个化合物,分别为角鲨烯(1),豆甾-4-烯-3-酮(2),豆甾-4-烯-3,6-二酮(3),8(17),13-ent-labdadien-15→16-lactone-19-oic acid(4),1-油酸单甘油酯(5),2α-羟基-乌索酸(6),山楂酸(7),2α,3α,23-三羟基乌苏-12,20(30)-二烯-28-酸(8),豆甾醇(9),7α-羟基谷甾醇(10),β-谷甾醇(11). 结论: 除化合物7外均为首次从该植物中分离得到.本研究对于深入了解认识香茶菜属植物地下部分的化学品质具有一定的帮助.     

中国南海聚裂丛柳珊瑚化学成分研究

目的研究聚裂丛柳珊瑚Rumphella aggregata的化学成分。方法利用硅胶色谱、Sephadex LH-20凝胶色谱、HPLC等手段对化学成分进行分离纯化;通过理化性质、波谱分析方法结合文献对照,鉴定化合物的结构。结果从聚裂丛柳珊瑚甲醇提取物中,共分离鉴定了15个单体化合物:胆甾醇(1)、(22E)-胆甾-5,22-二烯-3β-醇(2)、(22E)-麦角甾-5,22-二烯-3β-醇(3)、麦角甾-5,24(28)-二烯-3β-醇(4)、豆甾-5,24(28)-二烯-3β-醇(5)、柳珊瑚甾醇(6)、胆甾-7-烯-3β,5α,6β-三醇(7)、(22E)-胆甾-7,22-二烯-3β,5α,6β-三醇(8)、麦角甾-7-烯-3β,5α,6β-三醇(9)、(22E)-麦角甾-7,22-二烯-3β,5α,6β-三醇(10)、豆甾-7-烯-3β,5α,6β-三醇(11)、(22E)-豆甾-7,22-二烯-3β,5α,6β-三醇(12)、尿嘧啶(13)、咖啡因(14)、hydratoperidinin(15)。结论化合物1～15均为首次从该属柳珊瑚中分离得到,并首次对化合物hydratoperidinin(15)的1HNMR及13 CNMR信号进行了全归属。化合物12在10μg.mL-1浓度水平,对K562肿瘤细胞株的抑制率为39.17%。     

三七叶皂苷酶水解产物的提取分离及结构鉴定

目的：对三七叶皂苷酶水解产物进行提取分离和结构鉴定。方法：在正交设计优选的酶解条件下，利用β-葡聚糖苷酶水解三七叶皂苷制备酶水解产物；大孔树脂法提取、柱层析法分离酶水解产物、光谱法确定酶水解产物的化学结构。结果：得到2种酶水解产物，经鉴定分别为人参皂苷C-K和人参皂苷-Mc。结论：利用大孔树脂提取及柱层析分离技术，可以实现三七叶皂苷酶水解产物的分离与制备。     

抗凝血活性海洋硫酸多糖HP1-1的结构研究

目的 对抗凝血活性海洋硫酸多糖HP1-1的结构进行研究。方法 通过高效液相色谱(HPLC)、高效凝胶渗透色谱(HPGPC)、傅里叶变换红外光谱(IR)以及气质联用色谱(GC-MS)和核磁共振波谱(NMR)方法,对抗凝血活性海洋硫酸多糖HP1-1的结构进行解析。结果 海洋硫酸多糖HP1-1的分子量为297 kDa,总糖和硫酸基含量分别为78.1%和12.3%;HP1-1主要由葡萄糖组成,含有少量半乳糖、阿拉伯糖和甘露糖;HP1-1糖链中含有→4)-Glcp-(1→、→3)-Glcp-(1→、→3,4)-Glcp-(1→、→4)-Arap-(1→、→3,4)-Galp-(1→和→3)-Galp-(1→,硫酸基位于→4)-Glcp-(1→的C-3位和→3)-Galp-(1→的C-4位。 结论 海洋多糖HP1-1是主要由葡萄糖组成的硫酸多糖,含有多种糖基连接方式,为独特结构序列的新颖海洋硫酸甘露阿拉伯半乳葡聚糖。