CD4+CD25+调节性T细胞与系统性红斑狼疮病情活动性关系的研究

目的：检测系统性红斑狼疮患者外周血CD4^＋CD25^＋、CD4^＋CD8^＋调节性T细胞亚群，探讨其与疾病活动性、肾脏损伤、血清抗ds-DNA抗体及免疫球蛋白和补体C3含量的关系。方法：采用流式细胞术检测北京协和医院住院和门诊SLE患者（n=37）外周血CD4^＋CD25^＋T、CD4^＋CD8^＋T细胞群比例，以15例RA和15例SS组成自身免疫性疾病对照，30例健康体检者作为正常对照，观察调节性T细胞亚群与SLE患者疾病活动性指标SLEDAI、IgG、C3及血清抗ds-DNA抗体的关系。结果：①疾病活动期SLE患者外周血CD4^＋CD25^＋调节性T细胞群比例显著低于正常对照组（P〈0.01），疾病稳定期和风湿性疾病对照组与正常对照组结果差异无统计学意义。疾病活动期和稳定期SLE患者CD4＋CD8＋T细胞群比例都略高于正常对照组，但未发现结果差异有统计学意义（P〉0.05）。②疾病活动期SLE患者外周血CD4^＋CD25^＋T细胞比例及CD4^＋CD25^＋/CD4^＋值显著低于稳定期患者（P〈0.01）。SLE患者外周血CD4^＋CD25^＋/CD4^＋值与SLEDAI、补体C3呈低度相关（r分别为-0.491、0.368，P〈0.05），CD4^＋CD25^＋T细胞数量与SLEDAI呈负相关（r=-0.578，P〈0.05）。③SLE并发肾病组外周血CD4^＋CD25^＋T细胞群比例及CD4^＋CD25^＋/CD4^＋值显著低于非肾病组（P〈0.01；P〈0.05）。同一SLE患者治疗前后CD3^＋CD4^-CD8^-细胞和NK细胞降低，CD4^＋CD25^＋细胞、CD4^＋CD25^＋/CD4^＋值及CD8^＋T细胞增加，但未发现这些结果差异有统计学意义。本次研究未发现NK细胞、CD4^＋CD8＋T细胞、CD4^＋CD25^＋T细胞群比例在ds-DNA＋组与ds-DNA-组之间结果差异有统计学意义。结论：SLE患者外周血CD4^＋CD25^＋T细胞群比例与SLEDAI成负相关，与肾脏的损害也有密切关系，但与血清抗ds-DNA抗体产生的关系不明显。活动期SLE患者外周血CD4^＋CD25^＋T细胞减少，稳定期CD4^＋CD25^＋T细胞比例回升，因此推测CD4^＋CD25^＋T细胞的变化可能是导致疾病发生和病情发展及相关器官（如肾脏）损伤的关键环节之一。     

The response of heat shock proteins 25 and 72 to ischaemia in different kidney zones

 Induction of heat shock proteins (HSPs) following cell injury contributes to the protection of vital cell functions. It was, therefore, of interest to study the effects of transient renal ischaemia on the abundance and distribution of two HSPs, HSP25 and HSP72, in renal tissue using Western-blot techniques. Analyses were performed on the supernatant (HSP25, HSP72) and pellet (HSP25) of homogenates obtained from cortex (CX) and outer (OM) and inner (IM) medulla of the rat kidney immediately after 60 min of ischaemia followed by varying periods of reperfusion. Ischaemia of the left kidney caused HSP25 contents to decrease in CX, OM and IM by 73, 89 and 54% respectively, compared with the corresponding zones of the contralateral control kidney. This initial decrease in supernatant HSP25 was accompanied by an increased abundance of HSP25 in the pellet. Following reperfusion, HSP25 contents in the supernatant gradually increased in CX and OM, reaching, after 24 h, values that were 5.4- and 2.5-fold higher, respectively, than those in the control kidneys. After 7 or 14 days of reperfusion, HSP25 contents had not completely normalised in CX, but had reached control levels in OM. In IM, the HSP25 content remained below control throughout the entire reperfusion period. HSP72 (supernatant) was below the detection limit in the CX of the control kidney. Similar to the level of HSP25, that of HSP72 was also markedly lower in OM and IM immediately after ischaemia. The intrarenal distribution of HSP72 and the sequence of zonal changes in HSP72 contents were similar to those observed for HSP25. These results are compatible with the view that, during ischaemia and the initial reperfusion period, HSP25 migrates from the cytoplasmic compartment (supernatant) into the nucleus and/or associates with cytoskeletal structures. The observation that both HSP25 and HSP72 are transiently induced in CX and OM, but not in IM, may be explained by the fact that, while all kidney cells are exposed to ischaemic stress, only inner medullary cells experience a major postischaemic attenuation of osmotic stress. Received: 11 February 1997 / Received after revision and accepted: 26 March 1997     

Direct infant UV light exposure is associated with eczema and immune development

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社区呼吸康复治疗在慢性呼吸系统 疾病患者干预管理中的应用*

摘 要： 目的：探究中老年人颈动脉斑块与血清25羟维生素D （25-OH-D） 的相关性。 方法：选取2019年1月—2020 年 12 月自愿参与该研究的上海市浦东新区北蔡社区常住居民 412 人为研究对象,测定及记录其一般临床资料及血清 25-OH-D 等实验室检测结果。依据血管 B 超结果将研究对象分为有斑块组 268 人和无斑块组 144 人,比较两组人群血清 25-OH-D水平差异,用Pearson相关性分析各变量的关系,采用logistic回归分析颈动脉斑块形成的危险因素。结果：有斑块 组血清 25-OH-D 为 （45.18±18.71） nmol/L,无斑块组为 （56.12±19.54） nmol/L,两组差异有统计学意义 （χ2=5.573,P＜ 0.05）。相关性分析显示颈动脉斑块与收缩压、HbA1c、年龄呈正相关 （r值分别为0.388、0.119和0.128,P值均＜0.05）;与 血清 25-OH-D 呈负相关 （r=-0.365,P＜0.01）。血清 25-OH-D 是颈动脉斑块形成的独立相关因素 （OR=0.973,95%CI： 0.960,0.985,P＜0.05）。结论：低水平血清25-OH-D是颈动脉斑块形成的独立危险因素。     

Relationship between Serum 25(OH)D and Depression: Causal Evidence from a Bi-Directional Mendelian Randomization Study

The relationship between depression and vitamin D deficiency is complex, with evidence mostly from studies affected by confounding and reverse causality. We examined the causality and direction of the relationship between 25-hydroxyvitamin D (25(OH)D) and depression in bi-directional Mendelian randomization (MR) analyses using information from up to 307,618 white British participants from the UK Biobank and summary results from the SUNLIGHT (n = 79,366) and Psychiatric Genomics consortia (PGC 113,154 cases and 218,523 controls). In observational analysis, the odds of depression decreased with higher 25(OH)D concentrations (adjusted odds ratio (OR) per 50% increase 0.95, 95%CI 0.94–0.96). In MR inverse variance weighted (IVW) using the UK Biobank, there was no association between genetically determined serum 25(OH)D and depression (OR per 50% higher 0.97, 95%CI 0.90–1.05) with consistent null association across all MR approaches and in data from PGC consortium. In contrast, genetic liability to depression was associated with lower 25(OH)D concentrations (MR IVW −3.26%, −4.94%–−1.55%), with the estimates remaining generally consistent after meta-analysing with the consortia. In conclusion, we found genetic evidence for a causal effect of depression on lower 25(OH)D concentrations, however we could not confirm a beneficial effect of nutritional vitamin D status on depression risk.     

宜昌夷陵地区11656例0~6岁婴幼儿25-羟维生素D水平检测分析

目的为了研究宜昌市夷陵区0~6岁儿童维生素D营养状况及其与年龄、性别、季节之间的关系,以便为夷陵区儿童合理补充维生素D提供科学依据。方法对2017年1月至2018年7月来夷陵区妇幼保健院体检的0~6岁11656例儿童,采用荧光免疫层析方法进行末梢血25-(OH)D 3水平检测。结果该区11656例儿童末梢血25-(OH)D 3水平为(29.35±7.59)ng/mL,其中维生素D缺乏组566例(4.86%),维生素D不足组6579例(56.44%),维生素D充足组4511例(38.70%)。1岁以内婴儿组维生素D水平明显高于幼儿组和学龄前组,3岁以后儿童维生素D水平明显下降,各年龄组间差异有统计学意义(χ2=145.846,P<0.05)。不同季节儿童维生素D水平春季最高,冬季最低(χ2=504.007,P<0.05)。不同性别间儿童维生素D水平差异并无统计学意义(t=0.841,P>0.05)。结论我区0~6岁儿童维生素D水平大部分处于不足的状态,应增加该区儿童维生素D的摄入量,尤其加强学龄前组儿童维生素D的补充及冬季户外活动。     

A new interstitial deletion of chromosome No. 4 del(4) (q22::q25)

A female child is described with multiple anomalies including epicanthus, frontal bossing, short sternum, polydactyly, cleft of the larynx, renal cysts, and unusual dermatoglyphics. She died aged 3 months and was found to have a unique de novo deletion of chromosome No. 4 (q22-q25). This case is compared with other long arm deletions of 4q and reference made to assignment of genetic markers to chromosome No. 4.     

血清25-羟维生素D和白细胞介素-22对急性肺炎患儿病情的诊断价值

目的 探究血清25-羟维生素D[25(OH)D]和白细胞介素-22(IL-22)对急性肺炎患儿病情的诊断价值。方法 选取2021年3月至2022年3月自贡市第一人民医院收治的96例急性重症肺炎患儿作为重症肺炎组,另选96例普通肺炎患儿作为普通肺炎组、96例体检健康儿童作为健康对照组。采用化学发光微粒子免疫检测法检测血清25(OH)D水平,采用酶联免疫吸附法（ELISA）检测IL-22水平;采用Pearson法分析重症肺炎患儿血清25(OH)D、IL-22水平与急慢性健康状况评分Ⅱ（APACHEⅡ评分）、序贯器官衰竭评分（SOFA评分）的相关性;采用受试者工作特征（ROC）曲线评估25(OH)D、IL-22对重症肺炎患儿危重症的诊断价值。结果 重症肺炎患儿血清25(OH)D与APACHEⅡ评分及SOFA评分呈负相关（r=-0.521、-0.484,P<0.05）,血清IL-22与APACHEⅡ评分及SOFA评分呈负相关（r=-0.614、-0.419,P<0.05）。ROC曲线显示,25(OH)D对危重症诊断的曲线下面积（AUC）为0.745,IL-22对危重症诊断的AUC为...     

Potentiation of acute opioid-induced respiratory depression and reversal of tolerance by the calcium antagonist nimodipine in awake rats

Summary The interaction between sufentanil, a -opioid agonist, and the Ca²⁺ antagonist nimodipine on respiration and on the development of opioid tolerance in awake rats has been analyzed. Our previous work demonstrated that chronic treatment with nimodipine together with sufentanil increases the analgesic potency of the opioid 50 fold. Therefore, we have investigated whether the opioid-induced respiratory depression is potentiated in parallel with the analgesia. Ventilation was measured by the whole body plethysmographic method. In naive rats, sufentanil (10–80 g/kg) consistently induced a dose-dependent respiratory depression. Pretreatment with nimodipine (200 g/kg) potentiated this effect but to a lesser extent than it potentiated analgesia. After chronic administration of the opioid (2 g/h, 7 days) tolerance was manifested as a reduction in both the area under the time course curve and in the maximum effect. Nimodipine (1 g/h) administered concurrently with sufentanil for 7 days counteracted the tolerance to respiratory depression but no additional potentiation was observed. These results demonstrate that the interaction between nimodipine and sufentanil is not limited to antinociception but also exends to respiratory depression. However, compared with analgesia, the clinical relevance of a potential increase in opioid-induced respiratory depression by nimodipine may be negligible.Correspondence to: M. A. Hurlé at the above address     

消炎痛对中晚期原发性肝癌病人NK细胞及T淋巴细胞亚群的影响

观察中晚期原发性肝癌病人在口服呋喃氟尿嘧啶的基础上加用消炎痛 (吲哚美辛 ) (治疗组 )和不加用消炎痛 (对照组 )外周血自然杀伤 (NK)细胞及T淋巴细胞亚群的变化。结果发现 :治疗组治疗后NK细胞活性较治疗前及较对照组治疗后显著提高 (P <0 .0 5 ,P <0 .0 1) ,而T淋巴细胞亚群的变化差异无显著性。提示消炎痛能增强中晚期原发性肝癌NK细胞的活性