微小病变型肾病患者血、尿中可溶性白介素受体浓度的变化及意义

目的 :观察可溶性白细胞介素 受体 ( s IL- 2 R)在微小病变型肾病综合征 ( MCNS)病人血浆和尿液中浓度变化 ,探讨其在免疫发病中的意义。方法 :用 ELISA分析法检测 2 3例微小病变型肾病综合征患者发病及缓解期血、尿白介素 受体 ,同时用3 H- Td R掺入法检测患者淋巴细胞增殖情况。结果 :MCNS患者在发病期血、尿 s IL- 2 Rα明显增高 ,于缓解期恢复正常。与对照组相比 ,发病期间患者尿液中增高的白介素 受体与血清浓度不成比例地异常增高。同时还发现血清可溶性白介素 受体与 T细胞体外对 PHA的增殖反应呈负相关 ,缓解期恢复正常。结论 :可溶性白介素 受体可能为血清中抑制 T细胞克隆扩增的一种因子。     

肺癌患者血清sIL-2R、IL-6和TNF-α水平的测定及临床意义

目的:探讨血清sIL-2R、IL-6和TNF-α的表达水平与肺癌诊断、疗效判定及预后的关系.方法:双抗体夹心法测定肺癌患者血清中sIL-2R、IL-6和TNF-α水平,并进行化疗前后对比.结果:肺癌患者sIL-2R、IL-6和TNF-α的生物活性均高于正常对照组,化疗后sIL-2R、IL-6和TNF-α水平较化疗前明显降低,差异有显性著(P＜0.01).结论:sIL-2R、IL-6和TNF-α可作为肺癌诊断、疗效判定及预后评价的辅助性指标.     

大鼠种植性肝癌血清sIL-2R表达水平变化及意义

目的 了解大鼠种植型肝癌生成前后血清sIL-2R水平的动态变化及意义。材料与方法 采用直接注射法制作大鼠肝癌动物模型，应用CT或MRI评价大鼠种植型肝癌的影像学特点，应用免疫试剂盒检测大鼠荷瘤前后外周血sIL-2R表达水平的变化，并与对照组进行比较。结果 大鼠肝癌种植后7天，种植肝叶均可见类圆形、单发、瘤灶影，直径约0．6—0．8cm，CT平扫为低密度，增强后边缘强化；MRI扫描T1WI为低信号，T2WI则为高信号。荷瘤大鼠sIL-2R表达水平较对照组明显增高．且随肿瘤增大而进一步升高。结论大鼠种植型肝癌影像学特点及sIL-2R水平的动态变化与人相似，sIL-2R为肝癌疗效评价提供了一个有价值的指标。     

Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma

The signaling of interleukin (IL)‐23 and its receptor (IL‐23R) play a crucial role in the development of cancers. However, the clinical significance of human serum soluble IL‐23R (sIL‐23R) and its relationship with IL‐23 are still not explored in non‐small cell lung cancer (NSCLC). In our study, sIL‐23R was first identified in the serum of NSCLC patients, but not in healthy controls, by proteomics. The IL‐23R mRNA and protein were upregulated in NSCLC cell lines and tissues tested by quantitative PCR, western blot analysis and immunohistochemistry. The levels of sIL‐23R, IL‐23, and IL‐17 in 195 NSCLC patients’ serum were determined by ELISA, and high levels of sIL‐23R were significantly associated with advanced N stage (P = .039), clinical stage (P = .007), and poor 5‐year survival rate. In vitro, sIL‐23R was shown binding to IL‐23 and the balance could affect patients’ N and T stage, overall survival, and downstream cytokine IL‐17 in a potential antagonistic relationship. Although sIL‐23R, IL‐23, and IL‐17 were all associated with poor prognosis, only the sIL‐23R/IL‐23 ratio (hazard ratio, 1.945; 95% confidence interval, 1.147‐3.299; P = .014) was found to be an independent factor for prognosis. Therefore, we identified fragments of soluble cytokine receptor of IL‐23R with affinity ability to its natural ligand IL‐23 in NSCLC patients’ serum. The balance between the 2 antagonists can work as a potential prognostic serum marker.     

Impact of Bone Marrow Natural Killer Cells (NK); Soluble TNF-α and IL-32 Levels in Myelodysplastic Syndrome Patients

Background: Myelodysplastic syndromes (MDS) are complex clonal hemopoietic progenitor cell disorders that result from the evolution of aberrant clones which lead to leukemia. Disorders of the immune system serve important functions in the pathophysiology and progression of this disorder. This study aimed to assess the bone marrow natural killer cells percentage as well as soluble TNF-α and sIL-32 concentration levels in MDS patients. Methods: Bone marrow samples were obtained from 34 MDS; 12 MDS-AML and 10 controls. The percentage of total NK cells and mature NK cells were determined by flowcytometry. Bone Marrow soluble TNF-α and sIL-32 concentration levels were measured by ELISA. Results: The percentage of total NK and mature NK cells were significantly lower in MDS patients as compared to controls (p     

九代清源口服液增强反复呼吸道感染患儿免疫功能的临床实验研究

目的：探讨九代清源口服液在增强反复呼吸道感染患儿免疫功能中的作用。方法：将１００例ＲＲＩ患儿随机分为２组,即常规治疗组和九代清源口服液辅助治疗组（简称九代组）,分别于治疗前、治疗后抽取静脉血检测Ｔ淋巴细胞亚群（ＳＰ法）、ＮＫ细胞活性（ＭＴＴ法）、ｓＩＬ－２Ｒ（ＥＬＩＳＡ法）、ＩｇＧ、ＩｇＡ、ＩｇＭ（透比浊法）。结果：ＲＢＩ患儿治疗前ＣＤ３＾＋、ＣＤ４＾＋、ＣＤ４＾＋／ＣＤ８＾＋、ＮＫ细胞活性及ＩｇＧ、ＩｇＡ显著降低,ＣＤ８＾＋、ＩｇＭ和ｓＩＬ－６Ｒ显著升高常规治疗指标明显好转,与正常对照组比较,仍有显著差异（Ｐ〈０．０５）;九代组治疗后有所恢复,但与正常对照组比较均无显著性差异（Ｐ〉０．０５）。结论：九代清源口服液具有较强的免疫调节作用,可明显增强ＲＲＩ患儿的细胞和体液免疫功能。     

银屑病患者血清及皮损组织液sIL-2R检测及其临床意义

目的：探讨血清及皮损组织液可溶性白细胞介素２受体水平与银屑病活动和型别的关系。方法：采用双抗体夹心法测定不同类型银屑病患者治疗前后血清及皮损组织液ｓＩＬ－２Ｒ水平。结果;寻常型,红皮病型,脓疱型和关节型银屑病患者血清ｓＩＬ－２Ｒ水平在进展期均高于正常对照组,皮损消退后,则明显下降;     

煤工尘肺患者血清白细胞介素6及其可溶性受体的含量及临床意义

目的　探讨血清白细胞介素6(IL-6)和可溶性IL-6受体(sIL-6R)水平与煤工尘肺的关系及临床意义。方法　采用ELISA法检测56例煤工尘肺患者及126名有相同接尘史但未患尘肺的煤矿工人(对照组)血清IL-6及sIL-6R的水平。结果　尘肺患者血清IL-6平均水平为(14.0±13.1)pg/ml,高于对照组［(7.2±8.2)pg/ml］,差异有显著性(P<0.01)。多元线性回归分析表明,尘肺患者血清IL-6水平与尘肺期别呈正相关(r=0.451,P<0.01);尘肺患者血清sIL-6R水平与对照组的差异无显著性(P>0.05)。结论　IL-6可能参与了尘肺的发病过程,测定尘肺患者血清IL-6可能对病情监测有一定参考价值。     

食管癌患者放疗前后血清sIL-2R 的研究

 目的　探讨食管癌患者放疗前后血清 s IL- 2 R的变化及临床意义。方法　用双抗体夹心 ELISA法检测了 50例食管癌患者放疗前后血清 s IL- 2 R水平。结果　食管癌患者放疗前血清s IL- 2 R水平显著高于正常人 (P<0 .0 0 1 ) ,且随病情进展而升高。放疗后血清 s IL- 2 R水平较放疗前显著降低 (P<0 .0 1 )。存活二年的患者放疗结束时 ,血清 s IL - 2 R<80 0 u/ml与 80 0 u/ml两组有明显差异 (P<0 .0 1 )。结论　食管癌患者血清 s IL- 2 R明显增高 ,并与肿瘤进展 ,预后密切相关 ,可为肿瘤治疗疗效和判断预后的一个重要指标。