凋亡抑制蛋白Livin在结肠癌组织中的表达及与细胞增殖的关系

目的 探讨凋亡抑制蛋白Livin在结肠癌组织中的表达及与细胞增殖的关系.方法 选取结肠癌患者术后病理标本70例,采用S-P免疫组化法检测Livin基因的表达,并探讨其与患者性别、年龄、肿瘤大小、组织分化程度、浸润深度、淋巴结转移和远处转移等生物学行为及细胞增殖的关系.另取30例癌旁正常的结肠组织作对照.结果 结肠癌组织中Livin基因的阳性率明显高于癌旁正常结肠组织（X^2=12.19,P＜0.01）.Livin基因在结肠癌组织中表达水平与性别、年龄、肿瘤大小及组织分化程度无关（P＞0.05）,随原发病灶的浸润深度、淋巴结转移和远处转移呈明显上升趋势（P ＜0.01或P＜0.05）.Livin基因表达阴性的结肠癌组织中PCNA的细胞增殖指数明显低于Livin基因表达阳性的组织（P＜0.05）.结论 Livin基因不仅参与结肠癌的发病进展过程,而且还参与了结肠癌的局部侵袭和转移的病理过程.Livin不仅参与结肠癌细胞凋亡的调控,还促进了细胞的增殖过程,可作为大肠癌临床诊断、转移和放化疗疗效及预后评估的敏感指标.     

半夏的快速繁殖与胚状体诱导

采用单因素比较、均匀设计、正交设计等方法，考察了半夏愈伤组织和胚状体诱导以及植株再生的几个主要影响因素，结果表明外植体来源、激素种类和水平、基本培养基类别均对半夏愈伤组织的诱导有直接影响；影响胚状体诱导的主要因素为活性碳，此外，培养基种类、天然附加物对胚状体诱导也有一定影响；较低浓度的6-BA和NAA对半夏胚状体再生植株有利。     

血精素对伤口组织细胞增殖能力的影响

用血精素（XJS）观察对伤口组织愈合的影响。XJS对小鼠伤口组织的成纤维细胞（mFb）和人阴茎包皮术后的上皮细胞（hEC）进行培养、传代,以此实验模型研究XJS对伤口组织的愈合作用。用酶联免疫检测仪（Bio－Rad450型）,在570nm波长测定光吸收值,它能促进mFb和hEC的增殖,提高细胞培养液的光吸收值。XJS是通过对细胞的增殖作用而促进伤口愈合。XJS对伤口愈合的机理有待进一步研究。     

免疫荧光染色检测不同组织细胞微管蛋白-β的表达

为了探讨体外培养的正常及肿瘤细胞微管蛋白 β的表达 ,将肺成纤维细胞 ( 3T3)、胃癌细胞 (BGC 82 3)、神经母细胞瘤株 (SH SY5Y)、大鼠胶质瘤细胞株 (C6)、多形性胶质母细胞瘤株 (BT32 5)体外培养 ,免疫荧光细胞染色检测微管蛋白 β的表达。发现 :所有细胞均为微管蛋白 β免疫反应阳性。结果提示 :微管蛋白 β阳性反应可发生在增生活跃的培养细胞     

护肾固精方对大鼠系膜细胞增殖周期的影响

目的探讨中药复方护肾固精方对大鼠系膜细胞(MC)生物学行为的影响。方法采用体外培养大鼠MC和血清药理学方法,分为护肾固精方血清组、脂多糖(LPS)病理组及正常对照组,将不同浓度的作用物加入MC共同培养,培养后的MC制成单细胞悬液,经流式细胞仪检测后,计算出细胞周期中的G0/G1期、S期、G2/M期各时期的百分比及S期抑制百分比、细胞PI。结果不同浓度的作用物对MC增殖周期有不同影响。LPS血清能明显刺激MC增殖,表现为G0/G1期细胞百分比减以及S期细胞增加;细胞的S期抑制百分比明显下降,而细胞的PI则显著增高;中、高浓度的中药血清明显抑制MC增殖,表现为G0/G1期细胞百分比增加及S期细胞减以,细胞的S期抑制百分比明显增加,而细胞的PI则显著降低。并且使大量MC阻滞在G0/G1期,能诱导MC发生凋亡。结论护肾固精方治疗系膜增殖性肾炎等肾小球疾病的作用机理之一,可能与抑制MC增殖和促其细胞凋亡有关。     

Genotoxic evaluation of antiepileptic drugs by Drosophila somatic mutation and recombination test

The study examines the potential genotoxicity of three antiepileptic drugs (phenytoin sodium, pregabalin, gabapentin) using the wing somatic mutation and recombination test (SMART) in Drosophila melanogaster. Trans-heterozygous (two genetic markers mwh and flr) third-instar larvae of D. melanogaster were treated with different concentrations of the test compounds. A positive correlation was observed between total mutations and the number of wings with morphologically detectable mutations. The observed mutations were classified according to size and type of mutation per wing. Phenytoin clearly increased the frequency of total spots at all concentrations above 1.25 μg/ml. Gabapentin also increased the frequency of total spots at concentrations of 40 and 80 μg/ml. This study shows that phenytoin and gabapentin have genotoxic effects according to the SMART test; however, pregabalin displays lower genotoxicity in the SMAR assay when compared with the other two antiepileptics. The results also show that all AED concentrations lower the survival rate of the flies.     

乘子法测定复方替硝唑含漱液中二组分的含量

目的：用乘子法同时测定复方替硝唑含漱液中二组分的含量。方法：用乘子法的原理和计算步骤处理多波长吸收度数据。结果：模拟样品中醋酸氯已定、替硝唑的平均回收率分别为99．75％，100．17％，RSD分别为0．73％，0．42％（n=10），对3个批号实际样品的测定结果与标准方法吻合。结论：与标准方法相比．该方法具有简便、快速、易于实现自动分析的优点。     

双波长倍增差示法测定复方新诺明片含量

分别测定标准溶液和样品溶液在265及275 nm处的吸收度,根据标准溶液的吸收系数可同时计算出样品中 SMZ 和 TMP 的含量。SMZ 平均回收率与变异系数分别为100.8%,0.95%;TMP 为99.16%,0.82%。方法简便、快速。结果令人满意。     

Genotoxic evaluation of sodium fluoride in the Somatic Mutation and Recombination Test (SMART)

In this study, genotoxic effect of sodium fluoride (NaF) was investigated in Drosophila melanogaster Somatic Mutation and Recombination Test. Third-instar larvae trans-heterozygous for two genetic markers mwh and flr, were treated at different concentrations (2.5 μg/ml, 5 μg/ml and 10 μg/ml) of the test compounds. After the treatment the observed mutations were classified according to size and type of mutation per wing. For the evaluation of genotoxic effects, the frequencies of spots per wing in the treated series were compared to the control group, which is distilled water. NaF has genotoxic and toxic effects for concentrations of 5 and 10 μg/ml. The present study shows that NaF may have genotoxic and toxic effects.