“五联法”治疗腰椎间盘突出症疗效观察

目的:探讨"五联法"对腰椎间盘突出症的临床疗效。方法:腰椎牵引、颈腰痛Ⅰ号方加美洛昔康药物口服、TPD神灯加止痛散外用中药透皮技术、针灸治疗、天麻素注射液静点。结果:治愈40例,好转70例,未愈10例,总有效率为91.7%。结论:"五联法"治疗腰椎间盘突出症有较好效果。     

In vivo efficacy of Trachyspermum ammi anticalcifying protein in urolithiatic rat model

Ethnopharmacological relevance

Many medicinal plants have been employed during ages to treat urinary stones though the rationale behind their use is not well established. Recently, we have successfully purified an anticalcifying protein from the seeds of Trachyspermum ammi (L.) Sprague ex Turril (Umbelliferae) using oxalate depletion assay and deciphered its inhibitory activity against calcium oxalate crystal growth.

Aim

In this report, the antilithiatic activity of Trachyspermum ammi anticalcifying protein (TAP) was studied in urolithiatic rat model.

Methodology

Urolithiasis was induced by exposure of 0.4% ethylene glycol (EG) and 1.0% ammonium chloride (NH₄Cl) for 9 days. The efficacy of TAP was studied in another group given same dose of EG and NH₄Cl in addition to 2 mg/kg body weight of TAP. Further, we evaluated ability of TAP to inhibit the attachment of calcium oxalate (CaO_x) crystal in kidney tissue and studied the consequences of CaO_x adhesion on renal functioning and tissue integrity.

Results

The antilithiatic potential of TAP was confirmed by its ability to maintain renal functioning, reduce renal injury and decrease crystal excretion in urine and retention in renal tissues.

Conclusions

Thus, the present investigation suggests the potential of TAP in preventing calcium oxalate deposition and forms the basis for the development of antilithiatic drug interventions against urolithiasis.     

Detection of the expression of heat shock protein 70 using quantum dots as fluorescence probe in human renal carcinoma

Objective： To detect the expression of heat shock protein 70 （HSP70） in human renal carcinoma tissues and cultured ACHN cells by using quantum dots-tagged fluorescence technology and its significance. Methods： Using the fluorescence property of quantum dots, indirect immunofluorescence method and immunocytochemical method were used to detect the expression of HSP70 tagged by quantum dots in renal carcinoma tissues and ACHN cells cultured in vitro. Results： Confocal fluorescence microscopy showed that HSP70 were significantly expressed in renal carcinoma tissues and ACHN cells cultured in vitro characterized by homogeneous distribution of intensive salmon pink fluorescence. Compared with FITC tagging, quantum dots tagged fluorescence had good specificity and signal to background. There was no notable quenching after excitation by quantum dots for 30 rain. Conclusion： Quantum dots can be used to label subcellular proteins and have obvious advantages compared with the traditional fluorescence methods. The quantum dots-tagged fluorescence could be applied as a new method for clinical labeling detection.     

呼吸机相关性肺炎的病原菌调查及护理对策

目的通过对普通外科重症监护病房(SICU)呼吸机相关性肺炎的病原菌进行调查分析,探讨相应的护理对策。方法回顾性分析我院SICU2006年1～10月呼吸机相关性肺炎患者62例,对其113份痰培养结果进行分析统计。结果113份痰培养分离出102株病原菌,革兰氏阳性菌占38.24%,革兰氏阴性菌占36.27%,真菌占25.49%。常见病原菌依次是MRSA(24.51%)、白色念珠菌(16.67%)、凝固酶阴性葡萄球菌(9.80%)、铜绿假单胞菌(8.82%)、鲍曼氏不动杆菌(8.82%)。结论SICU呼吸机相关性肺炎的病原菌种类较多;针对其产生原因进行积极防治是减少呼吸机相关性肺炎的重要措施。     

心型脂肪酸结合蛋白在急性心肌梗死早期诊断中的价值

目的探讨血清心型脂肪酸结合蛋白(H-FABP)在急性心肌梗死(AMI)早期诊断中的临床应用价值。方法随机选择110例临床疑似AMI胸痛患者,采用时间分辨免疫荧光测定法(TRIFA)检测患者入院即刻血清中H-FABP含量,并与心肌肌钙蛋白I(cTnI)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、超敏C反应蛋白(hsCRP)和肌红蛋白(MYO)进行比较;对11例患者入院即刻和入院6 h后进行动态分析;以60例体检健康者作对照,绘制各心肌损伤标志物受试者工作特征(ROC)曲线并进行曲线下面积(AUC)比较,分析6种心肌损伤标志物诊断早期AMI的敏感度和特异度。结果 AMI患者入院即刻各心肌损伤标志物的AUC由大到小依次为H-FABP、hsCRP、cTnI、CK-MB、CK和MYO,最佳临界值诊断灵敏度分别为85.0%、78.7%、81.3%、73.8%、72.5%和61.3%,特异度分别为93.3%、95.0%、93.3%、100.0%、100.0%、98.3%。H-FABP的AUC与hsCRP、cTnI比较差异无统计学意义(P>0.05),与CK-MB、CK、MYO比较差异有统计学意义(P<0.05)。H-FABP诊断早期AMI的阳性率达85.0%。结论 H-FABP对于AMI早期诊断具有相对较早的检测窗口期和相对较好的特异度,在时效性、灵敏度和特异度等方面具有综合优势,可作为AMI早期诊断或排除诊断的血清标志物。多项心肌损伤指标联合检测可提高AMI实验室诊断的灵敏度、特异度及准确性。     

血清RBP4与妊娠期糖尿病胰岛素抵抗关系

目的探讨血清视黄醇结合蛋白4（RBP4）水平与妊娠期糖尿病（GDM）胰岛素抵抗的关系。方法采用酶联免疫吸附法测定了30例GDM病人（GDM组）及30例糖耐量正常孕妇（对照组）血清RBP4水平,葡萄糖氧化酶法检测空腹血糖（FPG）,放射免疫法检测空腹胰岛素（FIN）,计算胰岛素抵抗指数（HOMA-IR）。结果 GDM组血清RBP4水平显著高于对照组（t=8.298,P〈0.05）。GDM组FPG、FIN及HOMA-IR水平明显高于对照组（t=7.259～16.409,P〈0.05）。GDM组血清RBP4水平与HOMA-IR呈明显正相关（r=0.744,P〈0.05）。结论 GDM病人血清RBP4水平升高,可能参与了糖尿病胰岛素抵抗的发生。     

妊娠糖尿病病人血清FABP4的变化及意义

目的观察妊娠糖尿病（GDM）病人血清脂肪细胞脂肪酸结合蛋白（FABP4）的含量变化,探讨其与GDM发病的关系。方法 2008年5月—2009年4月,选择在我科行择期剖宫产术的GDM孕妇30例为GDM组,以同期行选择性剖宫产术的糖耐量正常孕妇30例为对照组。采用酶联免疫吸附法检测两组孕妇血清FABP4水平,放射免疫法检测空腹胰岛素（FINS）水平,葡萄糖氧化酶法检测空腹血糖（FBG）水平,计算稳态模型的胰岛素抵抗指数（HOMA-IR）,并对GDM组孕妇血清FABP4水平与HOMA-IR行相关性分析。结果 GDM组孕妇血清FABP4、FINS、FBG水平及HOMA-IR均显著高于对照组（t=5.80~23.01,P〈0.05）。GDM组孕妇血清FABP4水平与HOMA-IR呈显著相关性（r=0.595,P〈0.05）。结论 GDM孕妇血清中FABP4水平升高可能与GDM发病有关。     

Skp2通过调控Snaill水平对上皮性卵巢癌细胞EMT的作用研究

目的 探讨S期激酶相关蛋白2(Skp2)通过调控转录因子Snaill水平对上皮性卵巢癌细胞上皮间质转化(EMT)的作用.方法 分别检测15例上皮性卵巢癌组织和正常卵巢组织中Skp2和Snaill mRNA水平;体外培养SKOV3细胞,将其分为阴性对照组(只加脂质体)、Skp2干扰表达组(加Skp2干扰表达载体和脂质体)...     

儿童幽门螺杆菌相关霉素的检测及临床意义

目的探讨不同H.pylori菌株类型与小儿胃十二指肠黏膜疾病的关系,了解本地区儿童感染的H.pylori菌株类型。方法胃镜下活检及快速尿素酶试验诊断H.pylori感染及观察胃十二指肠黏膜病理变化,用免疫印迹法测定153例H.pylori阳性患儿血清多种H.pylori毒素抗体。结果胃炎患儿Ⅰ型H.pylori菌株为14例(27%),十二指肠球部溃疡患儿Ⅰ型H.pylori菌株为43例(74%),两组检出率比较差异有显著性。CagA抗体阳性者黏膜中重度炎性反应58例(92%),CagA抗体阳性者黏膜轻度炎性反应21例(35%),两组检出率比较差异有显著性。结论本地区儿童感染的H.pylori为Ⅰ型和Ⅱ型毒力菌株,CagA抗体阳性能引起胃十二指肠黏膜较重的炎性反应。     

痰热清注射液对机械通气患者肺保护作用的临床研究

目的:评价痰热清注射液对机械通气患者的肺保护作用,探讨中成药在防治机械通气相关肺损伤(VILI)和相关性肺炎(VAP)以及降低患者病死率的作用。方法:50例机械通气患者随机分为机械通气组(对照组,25例)和机械通气痰热清干预组(试验组,25例)。比较两组患者呼吸力学、动脉血气的变化;观察两组患者肺及肺外器官功能改善率、并发症发生率、重症监护室(ICU)病死率及其死亡的原因等。结果:两组患者的年龄和急性生理学与慢性健康状况评分(APACHE)比较,差异均无显著性(P均>0.05);试验组对患者的呼吸力学、动脉血气的影响均优于对照组;其肺及肺外器官功能的改善率明显优于对照组,另外VILI、VAP的发生率也明显下降;试验组因多器官功能衰竭的ICU病死率为16%,明显优于对照组的48%(P<0.05)。结论:机械通气患者经痰热清干预能改善其呼吸力学和动脉血气,并降低VILI、VAP的发生率,降低MODS机械通气患者的病死率。