磷脂双层膜(s-BLM)对ss-DNA的电化学响应研究

利用循环伏安法,以铂电极支撑的磷脂双层膜(s-BLM)作为生物膜的模型,研究了连接有芘(pyrene)和四氯荧光素(TET)的Oligo d(T20)对磷脂双层膜电化学行为的影响,结果显示s-BLM与Oligo d (T20)之间可以发生比较强烈的相互作用,导致了膜电压和膜结构发生了差异性改变,这种现象的出现可能是由于连接有芘和四氯荧光素的Oligo d(T20)与磷脂双层膜发生作用时引起s-BLM表面分子的排列变化,改变了膜的通透性,这为构建整合有ss-DNA磷脂双层膜生物传感器装置提供可能.     

Green voltammetric nano scaled determination of toldimfos and its residues in cattle meat and milk in presence of its toxic metabolite and Co-administered vitamin C

Our main focus is the green chemistry challenge, which is already a trend in all environmental processes. Voltammetry is an eco-friendly technique as it requires fewer solvents through minimum steps, producing no hazards. In the developed method, a novel electrochemical sensor was utilized to increase the sensitivity, selectivity, and enable minor detection of veterinary compound toldimfos sodium (TDS) in many matrices. The multi-walled carbon nanotube composite modified with zeolite (ZY/MWCNT) creates the sensor. A good linear relationship was obtained over a range of 0.2–100 nM with a high correlation of 0.9995. Its sensitivity was proved by the detection and quantification limits, which were calculated and found to be 50 pM and 151.5 pM, respectively. In order to test for food safety, it is utilized to determine the qualitative and quantitative amounts of TDS residue in cattle meat and milk. The obtained results were statistically compared with the reported method successfully. A greenness assessment was performed using Analytical Eco-Scale (AES) and Green Analytical Procedure Index (GAPI) tools, revealing an excellent green profile for the suggested method.