The effect of amino acids on intestinal smooth muscle related to their content in blood and tissue

The concentration of amino acids in the blood of the guinea pig is about 0.1 mM, while the intracellular concentration in the gastrointestinal smooth muscle cells is about ten times higher. Most amino acids, applied exogenously to isolated smooth muscles, exert effects in concentrations resembling their intracellular concentration. The results show that the inhibiting amino acids do not function as a neurotransmitter in the non-adrenergic inhibitory nervous system. The effective amino acids may possess modifying properties with respect to the contractile state of the muscle.     

癌胚抗原的测定对估计胃癌预后的意义:胃癌的免疫组织化学研究

对确诊为胃癌的63例,在手术后随访,以存活5年和不足3年为限分为预后较好组和较差组,自胃癌原发部位取组织用PAP法检测胃癌细胞内癌胚抗原(CEA),二者之间差异极为显著,CEA阳性程度高者,预后较差,反之,预后较好。因此CEA可作为估计胃癌预后的一个标志。     

Cephalic angled views for detection of occult lesser curvature ulcers

A total of 10 gastric ulcers in the lesser curvature, which would probably have been overlooked, were readily seen by using cephalic tube angulation in upper gastrointestinal (GI) radiography. Routine use of angled radiography in patients with a high transverse stomach is recommended to visualize lesser curvature ulcers which, due to the high position of the stomach, are easily missed at fluoroscopy and are not visible on routine films.     

胃癌相关分泌蛋白CA11与胃癌关系的研究

目的确定CA11编码蛋白的性质,细胞水平、动物体内的功能,并探索其发挥作用的可能机制,以及与其它已知抑癌基因、癌基因的调控关系。方法将CA11克隆至pcDNA3.1/Myc-His(-)A载体。CA11与空载稳定转染胃癌细胞株7901,细胞爬片后行抗Myc标签及8种抑癌基因、癌基因的细胞免疫组织化学分析。CA11与空载稳定转染胃癌细胞株7901,分别行生长曲线及裸鼠荷瘤实验,流式细胞仪分析细胞周期。结果含有Myc标签CA11编码蛋白存在于CA11转染的胃癌细胞浆、细胞膜上及膜外。CA11稳定转染胃癌细胞株7901及裸鼠荷瘤实验表明,CA11能显著抑制其生长犤21d,(292.29±28.76)mm3与(1330.50±110.07)mm3比较,P<0.01犦,转染CA11的胃癌7901细胞G165.5、S24.7,与对照组空载转染7901细胞G150.5、S37.1相比,G1期增多而S期减少。8种已知抑癌基因、癌基因在CA11与空载稳定转染胃癌细胞株7901均无表达。结论证实CA11编码蛋白为一分泌蛋白,在细胞水平及动物体内均能明显抑制胃癌细胞的生长,它与多种已知抑癌基因、癌基因并无相互调节的关系。     

Massive gastric distention due to duodenal involvement by retroperitoneal tumors

We present three patients with retroperitoneal tumor involving the duodenum in whom upper gastrointestinal (GI) studies revealed delayed gastric emptying and massive gastric dilatation out of proportion to the degree of duodenal dilatation. While the radiographic findings mimicked acute gastric outlet obstruction, delayed films demonstrated narrowing and encasement of the duodenum at the site of obstruction in all three patients. Thus, the duodenum should be carefully evaluated radiographically in patients with acute gastric dilatation, particularly if there is a known history of malignancy.     

我国1541例进展期胃癌临床病理观察

本文对我国胃癌高、低发区进展期胃癌(AGC)的临床和病理特点进行了观察,结果表明:①患者多为老年,男多于女(3∶1),病程短,多在2年内,症状多不典型;②癌多位于胃窦小弯,溃疡底部四层结构不典型,其中坏死层最显,瘢痕少,常有肌残留和癌浸润,组织类型以低分化腺癌和管状腺癌多见,癌细胞可显示不同程度异质性,越向深部浸润时可出现分化越低的倾向;③癌旁胃粘膜伴发病变的检出率及其程度均明显高于和重于同例非癌旁相应病变(P<0.005),提示前者与胃癌的发病关系更密切;④粘液组化染色证实肠型胃癌多见(P<0.005)。     

Gastric herniation and volvulus: CT and MR appearance

Organo-axial volvulus of the stomach, a potentially catastrophic situation, may be recognized as an incidental finding on computed tomography (CT). We present the CT features of organoaxial volvulus of the stomach in two cases. In one case, a correlative magnetic resonance (MR) image is presented.     

胃癌危险因素的Logistic回归分析

本文运用流行病学研究方法对187例原发性胃癌患者进行了不同因素的调查研究。将所获资料经回归程序处理。结果发现,胃炎、胃溃疡、食用咸鱼、熏制食品及杂粮等为发生胃癌的主要危险因素。     

人胃癌原代细胞培养化疗药物敏感性评价

目的:评价人胃癌原代细胞对7种化疗药物的敏感性。方法:取59例手术切除的新鲜胃癌组织标本,应用人胃癌原代细胞短期培养(混杂非肿瘤细胞)与纯化培养MTT法体外药敏试验,测定每种化疗药物对肿瘤细胞的抑制率。结果:药敏试验测定7种化疗药物抑制率除氨甲蝶呤(MTX)外纯化法均高于短期法,表明纯化法较敏感;两种方法测定药物敏感性顺序一致,由高至低依次为顺铂(DDP)、丝裂霉素(MMC)、阿霉素(ADM)、依托泊甙(VP-16)、5-氟尿嘧啶(5-FU)、羟基喜树碱(HCPT)、MTX。结论:7种化疗药物以DDP、MMC、ADM较敏感,VP-16、5-FU次之,HCPT、MTX最差。     

Incorporation of Human Chorionic Gonadotropin Alpha-Subunit into Different Types of Granule in Stomach Endocrine Cells

In order to demonstrate the ultrastructural localization of the α subunit of human chorionic gonadotropin (hCG) in the stomach mucosa, an immunoelectron microscopic study was performed using formalin-fixed specimens. In pyloric glands, α hCG-positive granules were irregular in outline, with a mean area and maximum diameter of 2.857 × 10⁴ nm² and 218.4 nm, respectively. In fundic glands, the granules had a smoother outline and were larger in both area and maximum diameter (3.943 × 10⁴ nm², 246.8 nm) than those of pyloric glands (p <0.001). In the atrophic fundic glands of non antral gastritis, the αhCG granules showed a differece in shape; more elliptical granules appeared to be increased, as indicated by the higher value of the axial ratio (1.452) and lower value of D (1.862) (log₁₀ area ∞ D log₁₀ perimeter) compared with those in pyloric (1.231, 1.968) and fundic glands (1.148, 1.979) (p< 0.001). The a-subunit of hCG is thus Incorporated into different types of endocrine cell in pyloric and fundic glands, and the granule morphology appears to differ in hyperplastic αhCG cells of non-antral gastritis. Acta Pathol Jpn 39: 737-742, 1989.