Vit B6在妊娠中期引产后抑制泌乳的探讨

目的:研究Vit B6在妊娠中期引产后抑制泌乳的作用及其临床应用.方法:60例妊娠中期孕妇经羊膜腔内注入雷夫奴尔100mg引产,其中30例术后不服药作为对照组,30例在术后2小时开始服VitB6 60mgtid×5天.两组均于术前及术后4天以RIA法测定血清中PRL、E2及P的水平,对结果作相关分析.同时观察乳汁分泌情况.结果:血清三项激素水平虽在术后均较前明显下降,但服药组PRL下降更为明显(P＜0.001 vs0.05),挤压乳房有泌乳者服药组仅2例,而对照组有9例.结论:妊娠中止后2小时内开始服VitB6能极为有效地抑制乳汁分泌(93.3%),可代替己烯雌酚等传统药物.血清PRL水平的显著下降(42.85%),提供了完善的实验室资料.     

大鼠模拟经前期综合征(PMS)肝气逆证动情周期血清激素水平的测定基金项目:国家中医药管理局资助课题 No:972035

本实验通过对雌性正常青年大鼠(未孕)和经脉冲电剌激5天造模大鼠血清激素的测定,结果发现:未孕正常大鼠血清中泌乳素(PRL)、雌二醇(E2)、孕酮(P)在动情期的前、中、后三期变化很显著(P<0.001);造模未孕大鼠三种激素除孕酮在中后期变化不显著、雌二醇在前中期较显著(P<0.01)外,其余均很显著(P<0.001),且三种激素与正常未孕相应期比除孕酮的中期变化不显著外,其余均变化很显著(P<0.001).     

男性不育症患者血清TNF-α、T、FSH、PRL检测的临床意义

目的：探讨男性不育症患者血清TNF-α、T、FSH、PRL水平的变化及临床意义。方法：应用放射免疫分析对36例男性不育症患者进行了血清TNF-α、T、FSH、PRL检测,并与35例男性正常人作比较。结果：男性不育症患者血清T水平非常显著地低于男性正常人组（P〈0.01）,而TNFα-、FSH、PRL水平又非常显著地高于男性正常人组（P〈0.01）,血清TNF-α水平与T水平呈负相关（r=-0.5184,P〈0.01）,与FSH、PRL水平呈正相关（r=0.6184、0.5925,P〈0.01）。结论：检测男性不育症患者血清TNF-α、T、FSH、PRL水平的变化对诊断、预后判断及治疗均有较大的临床价值。     

Neonatal hypothyroidism caused by maternal nicotine exposure is reversed by higher T3 transfer by milk after nicotine withdraw

Maternal nicotine exposure leads to neonatal hypothyroidism that can be returned to euthyroidism after nicotine withdrawal. Here, we examined the transfer of iodine through milk, deiodinase activities (D1 and D2), and serum T3, T4 and TSH in rat offspring after maternal exposure to nicotine. One day after birth, a minipump was implanted to dams releasing nicotine (NIC), 6 mg/kg/day for 13 days or vehicle saline. Animals were killed at the day 15 and 21 of lactation. At day 15, NIC-treated dams showed decreased T4 and mammary 2 h-radioiodine uptake (RAIU) and increase of TSH, thyroid 2 h-RAIU, liver D1 and mammary D2. At the cessation of NIC-exposure, pups displayed decreased T3, T4 and thyroid 2 h-RAIU and increased TSH. At weaning (21-postnatal day), NIC-treated dams recovered their T4 and TSH, but increased deiodinase level in the liver and mammary gland. Milk T3 content in NIC-treated dams was higher at both day 15 and 21, and thyroid function was recovered at the day 21. Thus, thyroid function was affected by nicotine in both mothers and pups, suggesting a primary hypothyroidism. After nicotine withdrawal, pups recovered thyroid function probably due to the increased lactational transfer of T3 in relation with increased mammary gland deiodinase activities.     

老年人群血清LH、PRL及睾酮变化规律的研究

应用RIA方法．检测627例血清睾酮（T）、促黄体生成素（LH）和泌乳素（PRL）水平．探讨各激素的增龄性变比。结果表明：血清T水平对照组为32．07±14．25nmol／L其它各组随增龄呈进行性降低．且与对照组相比存在显著差异（P＜005）：血清LH水平是随龄增高趋势．且与T变比呈显著负相关（r=－0．21，P＜0．01）。血清PRL水平亦呈现随龄增高趋势。PRL与T（r=－0．12．P＜0.05）、PRL与LH（r=0．11．P＜0．05）呈微弱相关关系。上述结果提示．在男性衰老过程中．T水平逐渐降低．对LH负反馈抑制作用减弱，而引致LH分泌增高．血清PRL对性腺轴激素水平有影响．但其具体作用及作用方式有待进一步研究。     

产妇乳汁PRL分泌影响因素的探讨

报道140例产后36～48、72～96小时的产妇乳汁中PRL 测定结果,表明乳汁中PRL 水平随产后时间的延长而逐渐下降,PRL 水平与泌乳量呈正相关。产后投子宫收缩剂麦角组的产妇乳汁中PRL 值低于投催产素组产妇乳汁中的PRL 值;分娩女婴的产妇乳汁中PRL 值高于分娩男婴产妇乳汁中PRL 值。但不同年龄、不同分娩方式及从事脑力或体力劳动的产妇乳汁中PRL 值未见显著差异。     

Effects of exogenous melatonin on pituitary hormones in humans

The effects of melatonin on physiological function remain unclear, although the therapeutic potential of melatonin is being increasingly recognized. The aim of the present study is to investigate the effects of exogenous melatonin on the spontaneous release of pituitary hormone in humans. A double blind placebo‐controlled protocol was designed to examine 12 adult healthy volunteers and 12 sleep disorder patients who have been treating with low doses of melatonin for 1 year. Either exogenous melatonin or placebo of 1 mg was given at 09:00 hours, followed by the collection of blood samples every 20 min for 4 h. Each blood sample was examined for levels of serum melatonin, PRL, LH, FSH, GH and TSH. LH levels were higher in sleep disorder patients compared with the healthy volunteers. In other pituitary hormones, there were no significant difference between healthy adults and sleep disorder patients. In all subjects, PRL levels were stimulated by acute administration of 1 mg of exogenous melatonin, while the levels of other pituitary hormones were not affected. These results suggested that exogenous melatonin can affect the spontaneous release of LH and PRL in humans. In addition, we demonstrated that 1‐year oral melatonin treatment did not affect the responses to the acute administration of melatonin.     

癫痫患者T淋巴细胞亚群和泌乳素的测定

为探讨神经内分泌免疫调节功能变化在癫痫发病中的作用，采用放射免疫法和ＡＰＡＡＰ法对４６例癫痫患者和３０例健康对照组血清泌乳素（ＰＲＬ）及外周血Ｔ淋巴细胞亚群进行了检测。结果显示：癫痫患者ＣＤ４细胞及ＣＤ４／ＣＤ８比值明显低于对照组（Ｐ＜００１，Ｐ＜０００１）；ＣＤ８细胞和ＰＲＬ含量显著高于对照组（Ｐ＜０００１，Ｐ＜００１），且上述指标的改变与癫痫的发作频率有关；ＰＲＬ与ＣＤ４／ＣＤ８之间呈显著负相关。提示：神经内分泌免疫调节功能失衡在癫痫的发病机制中起着重要的作用