早期肝癌中端粒酶活化与细胞增殖相关性研究

目的：研究早期肝癌中端粒酶基因蛋白(hTRT)与细胞增殖核抗原（PCNA）表达及其相关性。方法：在20例早期肝癌中用原位杂交检测hTRT基因,免疫组化方法检测PCNA的表达。结果：hTRT阳性信号检出率80．0％,PCNA强阳性率为55．0％;癌中hTRT阳性信号、PCNA表达强度显著高于癌旁非癌组织（P＜0．05）;癌不则分化组中PCNA表达有显著差异（P＜0．05）;癌中hTRT阳性信号分布有模型和浆型两种形式,其分布形式、 信号强度均与PCNA阳性强度密切相关（P＜0．01）。结论;肝癌发生与细胞增殖密切相关。端粒酶活化是肝癌发生的早期事件,是肝细胞增生和恶性转化所必需,其检出结果对鉴别肿瘤恶变有重要意义。     

骨肉瘤和骨巨细胞瘤的p53蛋白、PCNA表达 与肿瘤组织微血管密度的关系

探讨骨肉瘤和骨巨细胞瘤的ｐ５３蛋白、增殖细胞核抗原（ＰＣＮＡ）表达与癌组织内微血管密度（ＭＶＤ）的关系。方法;应用免疫组化和形态计量方法检测４０例骨肉瘤和４０例不同级别骨巨细胞瘤的ｐ５３蛋白、ＰＣＮＡ表达与肿瘤组织内ＭＶＤ之间的关系。结果：骨巨细胞瘤Ⅱ～Ⅲ级组ＰＣＮＡ表达明显高于骨巨细胞瘤Ｉ级组（Ｐ＜０．０５）,且骨巨细胞瘤ＭＶＤ≥５的肿瘤组织Ｐ５３蛋白表达明显高于ＭＶＤ＜５的肿瘤组织（Ｐ＜０．０５）;骨肉瘤ＭＶＤ≥５的肿瘤组织Ｐ５３蛋白表达也明显高于ＭＶＤ＜５组（Ｐ＜０．０５）。ｐ５３蛋白阳性率虽骨巨细胞瘤Ⅱ～Ⅲ级、骨肉瘤均高于骨巨细胞瘤１级但无统计学意义（Ｐ＞０．０５）。结论：ｐ５３蛋白、ＰＣＮＡ过度表达与ＭＶＤ有密切关系,ｐ５３、ＰＣＮＡ及ＭＶＤ可作为评估骨巨细胞瘤、骨肉瘤预后有价值的分子生物学指标。     

VEGF,bFGF及PCNA在宫颈癌中的表达及其与肿瘤增殖和血管生成的关系

目的 :探讨有关细胞因子在调控宫颈癌增殖及其血管生成中的作用。方法 :应用免疫组织化学及其组织形态学定量分析方法 ,对血管内皮细胞生长因子 (VEGF) ,碱性成纤维细胞生长因子(b FGF)和增殖细胞核抗原 (PCNA)在宫颈癌中的表达进行研究。结果 :1肿瘤细胞增殖与 VEGF的表达呈正向关系 ,而与 b FGF的表达无关 ;2 VEGF,b FGF的表达分别与微血管密度有正相关性 ,而当 VEGF和 b FGF共同表达时 ,微血管密度显著增加 ;3VEGF,b FGF在不同个体的表达具有异质性。结论 :VEGF有促进肿瘤细胞增殖及血管生成的作用。VEGF,b FGF在肿瘤血管生成过程中可能起协同作用     

PCNA含量及DNA含量联合检测在子宫内膜癌及癌前病变中诊断意义

目的：说明ＰＣＮＡ含量及ＤＮＡ含量联合检测在子宫内膜癌及癌前病变中的诊断意义;两种取材方法对结果的影响,以探讨临床工作采用新鲜标本作为早期诊断的辅助手段是可行的。     

大肠肿瘤中细胞核形态参数和p53、PCNA表达的定量研究

目的 研究大肠肿瘤中细胞核形态参数和ｐ５３、ＰＣＮＡ定量表达的关系。方法 应用免疫组化和图像分析技术对４０例腺瘤、８０例腺癌及１５例正常粘膜细胞核形态参数和ｐ５３、ＰＣＮＡ含量进行研究。结果 腺瘤及腺癌中核面积、核周长、核平均直径均大于正常粘膜（Ｐ〈０．０５,Ｐ〈０．０１）,腺癌组ｐ５３及ＰＣＮＡ相对含量均高于腺瘤组（Ｐ〈０．０５）。ＰＣＮＡ表达与大肠癌的差分化有关（Ｐ〈０．０５）。核面积及核平均     

增殖细胞核抗原和P糖蛋白在胃癌中的表达和意义

目的 :探讨增殖细胞核抗原 (PCNA)和P糖蛋白在判断胃癌预后、指导化疗中的意义。方法 :采用S -P免疫组化染色法。结果 :低分化腺癌PCNA阳性率 (97 4% )明显高于高分化腺癌PCNA阳性率 (75 0 % ) ,两者具有极显著性差异 (P <0 0 1 ) ;低分化腺癌的PCNA表达强度高于高分化腺癌 ,且差异具有显著性 (P <0 0 5 )。P糖蛋白表达与淋巴结转移及肿瘤细胞的恶性程度无关 (P >0 0 5 )。结论 :对胃癌病人进行PCNA、P糖蛋白检测 ,在判断预后、指导化疗中起重要作用     

P^21和PCNA在肝硬变组织中表达的研究

目的：探讨Ｐ２１、ＰＣＮＡ的相关关系及它们在肝硬变、肝细胞不典型增生（ＬＣＤ）、肝细胞癌（ＨＣＣ）形成中的作用。方法：运用Ｓ－Ｐ法对６０例单纯肝硬变（不伴癌）、３０例癌旁肝硬变及２７便ＨＣＣ的Ｐ２１、ＨＢｓＡｇ及ＰＣＮＡ表达情况进行实验研究。结果：癌旁肝硬变组织、单纯肝硬变组织、癌组织Ｐ２１阳性率分别为９０％、６８．３３％、６２．９６％,癌旁肝硬变与单纯肝硬变,癌旁肝硬变与癌组织之间判别有显著性意     

反义PCNA与反义bcl-2脱氧寡核苷酸抑制人视网膜色素上皮生长

目的　观察反义增生细胞核抗原 (PCNA)和反义 bcl-2脱氧寡核苷酸对培养的人视网膜色素上皮细胞 (RPE)生长的作用 .方法　用 DNA合成仪合成有 1 8个核苷酸的反义PCNA和 1 5个核苷酸的反义 bcl- 2脱氧寡核苷酸片段 ,以不同浓度加入 RPE培养液 ,培养 2～ 4d,用 SABC法检测细胞PCNA和 bcl- 2的表达 ,用 MTT法及流式细胞仪测定细胞抑制率 .结果　 6 .2 5～ 1 0 0μm ol· L- 1 的反义 PCNA对 RPE的抑制率为 8.3%～ 38.4% ,呈剂量依赖性 ,并抑制细胞 PCNA的表达 .反义 bcl- 2未显示抑制作用 .结论　反义 PCNA脱氧寡核苷酸可抑制近 40 %的 RPE生长 ,其临床应用值得进一步研究     

PCNA immunostaining combined with AgNOR staining in esophageal squamous cell carcinoma to identify patients with a poor prognosis

Immunostaining of the proliferating cell nuclear antigen (PCNA) provides important information about cell kinetics and is easily performed on routinely obtained formalin-fixed, paraffin-embedded materials. We report herein the results of a retrospective study on PCNA staining in esophageal cancer undertaken to determine its significance. As this study indicated that immunoreactivity was preserved in specimens fixed within 24 h, only 31 specimens from surgical patients were available for this investigation. The mean PCNA index of the patients without invasion to the adventitia (35.7±17.9) was significantly lower than that of those with invasion to the adventitia or neighboring structures (49.7±14.5), while the PCNA index did not correlate with other clinicopathologic parameters such as histologic type, lymph node metastases, or prognosis. However, when an analysis of PCNA staining was combined with an analysis of argyrophilic nucleolar organizer region (AgNOR) staining, a correlation with prognosis was found. In fact, seven patients with a high PCNA index (44) and AgNOR count (6) had a significantly poorer prognosis than the remaining 22 (P=0.0014), and six of these seven patients died within 2 years. These results indicate that this combined evaluation may be useful for the identification of patients with a poor prognosis among those undergoing surgery for esophageal squamous cell carcinoma.     

The anti-proliferative effect of proliferating cell nuclear antigen-specific antisense oligonucleotides on human gastric cancer cell lines

The proliferating cell nuclear antigen (PCNA) is a nuclear protein that leads DNA synthesis by the DNA polymerase delta. As the PCNA gene is strongly expressed in invasive gastric cancer cells with high proliferative activity, PCNA is suspected of playing an important role in the proliferation and advancement of gastric cancer. Thus, the effects of antisense oligonucleotides specific for PCNA mRNA were examined in seven gastric cancer cell lines. It was found that treatment with antisense oligonucleotides at concentrations of 10–40 M dose-dependently inhibited the growth of all cell lines; however, random sequence oligonucleotides did not modify the proliferation of any type of cells. These results indicate that PCNA is essential for cell proliferation in gastric cancer cells, and that the growth inhibitory effect results from the inhibition of PCNA gene expression. Therefore, PCNA-specific antisense oligonucleotides may be effective in the treatment of gastric cancer.