男性不育症患者AZF基因与临床相关性研究

目的探讨不育症患者中无精因子基因家族（AZF）缺失类型分布及其与临床症状的关系。方法采用多重PCR技术对15个序列标记位点（STS）进行检测,判断不育患者的微缺失类型。结果运用序列标记位点在418例不育患者中检出Y染色体微缺失43例,缺失率10．3％,对照组无缺失病例检出。结论大多数AZFa区缺失临床上可表现唯支持细胞综合征,AZFb区域缺失影响精子的发育,AZFc区域缺失表现出临床症状异质性,AZFd缺失临床症状较轻或精子仅表现形态异常。     

男性精神分裂症烟草依赖者个性特征的对照研究

目的 探讨精神分裂症烟草依赖者的个性特征.方法 采用辽宁省教育科研所修订的卡特尔16种人格因素(16PF)测试量表对2006年1～4月份在我们医院精神科封闭式住院病房的男性吸烟非吸烟分裂症住院患者进行16PF测查.研究对象在合作状态自行评定.两组16PF采用粗分与辽宁省成人男性常模粗分比较.结果 男性吸烟与非吸烟精神分裂症住院患者16PF比较无差异(P＞0.05).男性吸烟精神分裂症住院患者的16PF结果中有8个因素与常模比较差异有显著性.结论 吸烟与非吸烟男性精神分裂症住院患者具有相似的个性因素,表现为紧张、世故、独立性差等个性特点.     

高压氧促进成年大鼠神经干细胞的增殖

目的:研究高压氧对成年大鼠神经干细胞增殖的影响。方法:采用BrdU免疫组化方法,观察高压氧对成年大鼠神经干细胞增殖的影响,同时通过脑内注射内皮素制备大脑中动脉闭塞(MCAO)模型,对这两种诱导神经干细胞增殖的处理加以比较。结果:高压氧暴露10d,侧脑室前角和外侧壁的BrdU~ 细胞数量显著增加,BrdU~ 细胞核计数显著多于正常组,阳性细胞核反应性也显著增强,多为深染的棕黑色细胞核,其效应与MCAO第8天对BrdU~ 细胞的效应类似;高压氧暴露10d也引起了海马齿状回颗粒细胞层BrdU~ 细胞数量显著增加。结论:反复高压氧暴露可能具有调节脑内神经干细胞增殖与分化的作用。     

Effects of preventive acupuncture or moxibustion at the Guanyuan acupoint on expression of interleukin-2 and its receptors in hypothalamus, serum, and uterus of menopausal rats **☆

BACKGROUND： Previous studies have indicated preventive acupuncture and moxibustion at the Guanyuan point have positive effects on the immune system of menopausal rats. OBJECTIVE： To observe the effects of preventive acupuncture or moxibustion at the Guanyuan point on interleukin-2 （IL-2） and its receptor （IL-2R mRNA） levels in hypothalamus, serum, and uterus of menopausal rats. DESIGN： Randomized control animal experiment. SETTING： School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine. MATERIALS： This study was performed at the Laboratory of Beijing University of Chinese Medicine from November 2004 to July 2005. The vaginal smear method was used to determine the estrus cycle of 9.5-month-old Sprague Dawley （SD） female rats. Three continuous estrus cycles were measured, and 160 rats with estrus cycle disorder, weighing （300 _＋ 20） g, were included in this study. The rats were randomly divided into four groups： preventive acupuncture group （n =48）, preventive moxibustion group （n =48）, model groups （n =48）, and 10-month-old group （n =16）. Sixteen 3.5-month-old healthy SD female rats, weighing （240 ± 20） g, were also assigned to the young group. All rats were clean grade and were purchased from the Laboratory Animal Center, Beijing University of Chinese Medicine. The experiment was in accordance with animal ethics standards. METHODS： Acupuncture was applied to the preventive acupuncture group at 10 months of age. The needle was inserted upward and perpendicularly two fen to a point that was two fen below the Guanyuan point. The rats freely moved around during needle retention （20 minutes）. Acupuncture was administered twice a week and continued for eight weeks. A moxa cone （small） moxibustion was applied to the preventive moxibustion group at 10 months of age. The rats were caught gently and kept at the same position as the preventive acupuncture group. The moxa cone was ignited and held to the Guanyuan point directly. Only one moxa     

电压门控钾通道Kv1.1在戊四唑致痫大鼠中的表达

目的通过检测戊四唑(PTZ)致痫大鼠钾通道Kv1.1蛋白表达,探讨钾通道Kv1.1与癫痫发病的相关性。方法40只SD大鼠分成实验组30只和正常对照组10只。实验组30只大鼠通过腹腔注射PTZ建立全身强直-阵挛发作大鼠癫痫模型,取成功致痫鼠24只均分成3组,分别于致痫后3个时间段(1h、24h、48h)取脑组织。用免疫组化法和Westernblot法检测大鼠钾通道Kv1.1蛋白。结果实验组大鼠海马区钾通道Kv1.1蛋白表达水平在致痫后3个时间段(1h、24h、48h)均明显低于对照组(P0.05),但3个时间段之间钾通道Kv1.1蛋白表达水平差异无统计学意义(P0.05)。结论大鼠海马区钾通道Kv1.1表达的减少与全身强直阵挛发作大鼠癫痫发病密切相关。     

穴位埋线治疗非酒精性脂肪性肝病实验研究

目的:探讨穴位埋线法治疗非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)的作用.方法:将30只SD雄性大鼠随机分为对照组、模型组和埋线组,每组各10只.对照组以普通饲料喂养,模型组和埋线组均以高脂饲料喂养8周,复制NAFLD模型.模型复制成功后,埋线组以温脾化湿法为治...     

吗啡依赖模型大鼠脑cDNA文库的构建

目的:构建吗啡依赖模型大鼠脑cDNA文库.方法:建立大鼠吗啡躯体依赖模型后,取鼠脑进行总RNA的抽提、mRNA的纯化,以mRNA为模板合成第一链cDNA后用LD-PCR方法合成双链cDNA,用限制性内切酶SfiⅠA、B分别双酶切双链cDNA与pTRG载体,连接后的cDNA文库转化XL1-Blue MRF′ Kan 超级感受态并收菌,完成cDNA文库构建.结果与结论:PCR结果显示,cDNA片段连接效率＞90%,cDNA插入片段在1 kb左右.吗啡依赖大鼠脑cDNA文库总容量为3.05×106,满足下一步细菌双杂交文库筛选要求.     

两种不同溶液体系提取大鼠脊髓蛋白双向电泳图谱比较

目的：比较尿素／CHAPS和尿素／硫脲／CHAPS／SB3－10两种不同溶液体系提取大鼠脊髓蛋白双电泳（two-dimensional electrophoresis,2-DE)图谱的差异。方法：大鼠脊髓经三氯醋酸／丙酮沉淀粉蛋白后,分别用尿素／CHAPS和尿素／硫脲／CHAPS／SB3－10两种不同的溶液体系提取蛋白。以固相PH梯度（IPG）等电聚焦为第一向,SDS－PAGE水平电泳为第二向进行蛋白质2－DE。图像分析软件ImageMaster 2D Elite3.01分析蛋白质2－DE图谱。结果：两种蛋白提取溶液分别获得1059和1023个蛋白（亚基）斑点,其中790个蛋白在两张图谱中重叠,其余269和233个蛋白点为两种体系各自所特有。两种提取方法共获得1292个蛋白斑点。结论：综合使用不同蛋白提取方法有助于提高组织蛋白2－DE图谱的完整性。