全文获取类型
收费全文 | 23854篇 |
免费 | 1961篇 |
国内免费 | 1125篇 |
专业分类
耳鼻咽喉 | 239篇 |
儿科学 | 126篇 |
妇产科学 | 470篇 |
基础医学 | 811篇 |
口腔科学 | 170篇 |
临床医学 | 2106篇 |
内科学 | 1513篇 |
皮肤病学 | 234篇 |
神经病学 | 533篇 |
特种医学 | 539篇 |
外国民族医学 | 6篇 |
外科学 | 1593篇 |
综合类 | 4611篇 |
现状与发展 | 2篇 |
预防医学 | 937篇 |
眼科学 | 804篇 |
药学 | 7799篇 |
27篇 | |
中国医学 | 3822篇 |
肿瘤学 | 598篇 |
出版年
2024年 | 70篇 |
2023年 | 249篇 |
2022年 | 488篇 |
2021年 | 750篇 |
2020年 | 692篇 |
2019年 | 580篇 |
2018年 | 795篇 |
2017年 | 867篇 |
2016年 | 983篇 |
2015年 | 885篇 |
2014年 | 2005篇 |
2013年 | 1852篇 |
2012年 | 2075篇 |
2011年 | 2163篇 |
2010年 | 1616篇 |
2009年 | 1334篇 |
2008年 | 1219篇 |
2007年 | 1281篇 |
2006年 | 1167篇 |
2005年 | 1019篇 |
2004年 | 744篇 |
2003年 | 657篇 |
2002年 | 574篇 |
2001年 | 469篇 |
2000年 | 354篇 |
1999年 | 305篇 |
1998年 | 231篇 |
1997年 | 196篇 |
1996年 | 170篇 |
1995年 | 149篇 |
1994年 | 134篇 |
1993年 | 96篇 |
1992年 | 70篇 |
1991年 | 97篇 |
1990年 | 79篇 |
1989年 | 53篇 |
1988年 | 59篇 |
1987年 | 42篇 |
1986年 | 29篇 |
1985年 | 59篇 |
1984年 | 50篇 |
1983年 | 36篇 |
1982年 | 37篇 |
1981年 | 32篇 |
1980年 | 17篇 |
1979年 | 27篇 |
1978年 | 23篇 |
1977年 | 17篇 |
1976年 | 11篇 |
1975年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
111.
112.
目的 比较常频机械通气(CMV)、猪肺磷脂注射液联合CMV、猪肺磷脂注射液联合高频振荡通气(HFOV)治疗新生儿呼吸衰竭的疗效。方法 选择2019年1月至2020年12月南阳市中心医院收治的133例呼吸衰竭新生儿为研究对象,根据治疗方式将患儿分为对照组(n=41)、观察组A(n=45)和观察组B(n=47)。对照组患儿给予单纯CMV治疗,观察组A患儿给予猪肺磷脂注射液联合HFOV治疗,观察组B患儿给予猪肺磷脂注射液联合CMV治疗。比较3组患儿机械通气时间、用氧时间、治疗前后的呼吸力学指标[动脉血氧分压(PaO2)、动脉血二氧化碳分压(PaCO2)、氧合指数(OI)、呼吸指数(RI)]和血清内皮素-1(ET-1)、肌酸激酶同工酶(CK-MB)、Ⅱ型肺泡表面抗原-6(KL-6)、Clara细胞蛋白16(CC16)水平及不良反应发生情况。结果 3组患儿的机械通气时间和用氧时间比较差异有统计学意义(F=12.658、26.196,P<0.05);观察组A和观察组B患儿的机械通气时间和用氧时间显著短于对照组(P<0.05),观察组A患儿的机... 相似文献
113.
Cannabinoids have been shown to influence food intake, and until recently, the neural pathways mediating these effects have remained obscure. It has been previously shown that intracerebroventricular injection of delta-9-tetrahydrocannabinol (Δ9-THC) causes increased consumption of palatable foods in rats, and we postulated the involvement of the hindbrain in this cannabinoid-induced food intake. Cannulated rats (both female and male groups) trained to consume sweetened condensed milk received either lateral or fourth ventricle injections of CP 55,940 and were presented with sweetened condensed milk 15 min after injection. Rats were injected over a range of doses between 100 pg and 10 μg per rat. Milk intake was recorded for a total of 3 h. Lateral ventricle injection of CP 55,940 increased milk intake at doses in the microgram range. However, CP 55,940 was effective in increasing food intake at nanogram doses when injected into the fourth ventricle. Finally, male rats appeared to be more sensitive to CP 55,940 than female rats inasmuch as milk consumption was increased at the 1 ng dose in male rats, whereas only the 10 ng dose was effective in females. These results indicate that CP 55,940 may act in the hindbrain to influence feeding behavior in rats. 相似文献
114.
115.
Parvalburnin是细胞内一种钙结合蛋白。同时又可作为中枢神经系统内与GABA共存的神经元亚群的特异标记物,主要标记篮状及苔烛细胞。用PAP方谈染色可见大鼠Parvalbumin免疫阳性神经终末在运动皮层锥体神经元胞体周围形成包篮现象,但因该方法的局限性.较难明确二者的关系。为进一步了解Parvalbumin阳性终未在锥体神经元脑体、树突与轴突整体上的分布状况以及运幼皮层内不同传出神经元是否均接受同样的支配,本实验利用FastBlue送行标记、固定脑片细胞内注入LueiferYellow结合免疫荧光、Confocal显微镜观察,研究运动皮层内皮质丘脑(束旁核)、皮质效状体及皮质脊髓三种投射神经元与Parvalbumin阳性终末的关系。通过1.μ连续扫描图像的分析及立体对观察,Parvalbumin阳性终末清晰可见,与LuciferYellow标记的锥体细胞的关系也容易辨别.在三种投射神经元胞体上均可见Parvalbumin阳性终末包绕,形成明显包篮现象,但三种神经元上的终末数未见明显区别·阳性终未还分布于近端树突上,距胞体越远越稀疏:但在距脑体50μm以上的顶树突、30μm以上的基树突及其二、三级分枝的远端树突上仍偶有终末分布.此外,三种神经元轴突起始段上也有少量终末接触,但未形成明显的cartridge现象.这一结果揭示,Parvalb 相似文献
116.
In the identified neurons B1, B2 and B3 of Helix pomatia an intracellular injection of Na+ induced an outward current in 10% and an inward current in 90% of the experiments. The outward current was associated with an increase and the inward current with a decrease of the membrane conductance. Both currents reversed at membrane potentials of between -60 and -70 mV. Inward currents were also elicited by intracellular Li+ or tris-[hydroxymethyl]-aminomethane (Tris+) injection. All inward currents were reduced by extracellular administration of tetraethylammonium or quinine. It is suggested that the outward current represents a calcium-activated potassium current and that the inward current is due to a blockade of potassium channels from the intracellular side. 相似文献
117.
Ron-El R Strassburger D Gelman-Kohan S Friedler S Raziel A Appelman Z 《Human reproduction (Oxford, England)》2000,15(8):1804-1806
The birth of 12 healthy infants to fathers with non-mosaic Klinefelter's syndrome has been reported so far. The spermatozoa for these pregnancies was obtained from frozen-thawed ejaculate in one pregnancy (twins) and from the testis in the remaining 10 infants. All of them had a normal karyotype. We describe a patient with non-mosaic Klinefelter's syndrome from whom a testicular biopsy was obtained and motile spermatozoa were collected. Of 16 oocytes that were injected, 14 fertilized and cleaved. Three embryos were transferred, resulting in a triplet pregnancy. Karyotype analysis from chorionic villous sampling revealed 46,XX, 46,XY and 46,XXY from the three fetuses. The affected 46,XXY fetus was reduced on the 14th gestational week. The pregnancy culminated with the birth of a healthy male and female, on the 36th gestational week, weighing 3600 and 2660 g respectively. This case report proves the presence of hyperploid spermatozoa in the seminiferous lumen, and strengthens the necessity of genetic diagnosis of the embryos or fetuses in such pregnancies to fathers with non-mosaic Klinefelter's syndrome. 相似文献
118.
Kahraman S Akarsu C Cengiz G Dirican K Sözen E Can B Güven C Vanderzwalmen P 《Human reproduction (Oxford, England)》1999,14(3):726-730
In this study the fertility and outcome of intracytoplasmic sperm injection (ICSI) using megalohead spermatozoa from the ejaculates and testicles was evaluated. Seventeen males with megalohead and pinhead sperm forms in their ejaculate were studied in 22 cycles. A high number of sperm heads without tails and abundant round spermatid forms were commonly observed. Round-headed spermatozoa were seldom accompanied by these severely abnormal spermatozoa. The majority of megalohead spermatozoa were observed to have multiple tails, were predominant in the sample, and were used for ICSI. Ejaculated megalohead spermatozoa were used for ICSI in 15 cycles, while testicular spermatozoa were used in seven cycles where there were no vital spermatozoa or spermatozoa of low vitality in the ejaculate. The same abnormal morphology was observed in the testicles as in the ejaculated spermatozoa in the same males. Mean (+/- SD) low motility 4.7 +/- 5.6% and sperm count (3.8 +/- 4.19 x 10(6)) were common findings in these severely teratozoospermic patients. A low fertilization rate (43.2%) was achieved by using megalohead sperm forms (group I, n = 17) in comparison with the control group (60.2%) which had zero normal sperm morphology according to strict criteria (group II, n = 30) (P <0.01). Furthermore, a low pregnancy rate (9.1%) was obtained in the megalohead sperm group in comparison with the control group (40%) (P <0.05). Low fertilization and pregnancy rates may be due to a high incidence of chromosomal abnormalities from severely defective spermatozoa in the ejaculate. Couples should be counselled and warned about possible low fertilization and pregnancy rates with ICSI when only pinhead and megalohead forms with a high number of sperm heads without tails are present in the ejaculate. 相似文献
119.
120.
Steger K Failing K Klonisch T Behre HM Manning M Weidner W Hertle L Bergmann M Kliesch S 《Human reproduction (Oxford, England)》2001,16(4):709-716
During spermiogenesis, histone-to-protamine exchange causes chromatin condensation. Spermatozoa from infertile men are known to exhibit an increased protamine-1 (PRM1) to protamine-2 (PRM2) protein ratio. Since patients undergoing testicular sperm extraction (TESE) followed by intracytoplasmic sperm injection (ICSI) reveal low fertilization rates, whether the outcome of ICSI could be related to the percentage of round spermatids expressing PRM1-mRNA and PRM2-mRNA was investigated. Applying in-situ hybridization, 55 testicular biopsies from men undergoing TESE/ICSI were investigated. The percentage of PRM1-mRNA and PRM2-mRNA positive spermatids was significantly (P < 0.0001) decreased in men with at least qualitatively normal spermatogenesis (PRM1-mRNA: 58.4 +/- 13.8%; PRM2-mRNA: 56.4 +/- 11.3%) and impaired spermatogenesis (PRM1-mRNA: 32.6 +/- 10.8%; PRM2-mRNA: 31.7 +/- 11.1%) compared with men with obstructive azoospermia and quantitatively normal spermatogenesis (PRM1-mRNA: 79.9 +/- 4.6%; PRM2-mRNA: 78.1 +/- 5.7%). A positive correlation (r(PRM1) = 0.733; r(PRM2) = 0.784; P < 0.001) was demonstrated between the score and the percentage of PRM1-mRNA and PRM2-mRNA positive spermatids. While successful fertilization was neither related to the score, nor to the percentage of PRM1-mRNA and PRM2-mRNA positive spermatids, a significant (P < 0.05) relationship was demonstrated between successful fertilization and the PRM1-mRNA to PRM2-mRNA ratio. Therefore, the PRM1-mRNA to PRM2-mRNA ratio in round spermatids may serve as a possible predictive factor for the outcome of ICSI. 相似文献