非霍奇金淋巴瘤中p73基因甲基化与mRNA表达的相关性研究

目的：探讨NHL中p73基因启动子甲基化与其mRNA表达的相关性。方法：用甲基化特异性PcR（MsP）方法对24例非霍奇金淋巴瘤（NHL）p73基因启动子的甲基化状况进行检测,用RT—PCR检测其1TIRNA表达。结果：24例NHL中21例发生甲基化,甲基化阳性率87．5％;对照组均未出现甲基化。24例NHL中20例p73mRNA阴性表达,占83．33％,对照组p73mRNA表达阳性。结论：在NHL中p73tuRNA表达下调,且p73基因表达与p73基因启动子的甲基化呈显著负相关;p73基因启动子的甲基化可能是造成p73基因表达下调进而导致NHL发病的原因之一。     

肺癌细胞株E - cadherin的表达状态及其与启动子甲基化的关系

[目的]研究肺癌细胞株上皮钙粘蛋白(E - cadherin)的表达与基因启动子甲基化状态的关系.[方法]采用免疫细胞化学法检测肺癌细胞株NCI - H460、SPCA和A549的E- cadherin蛋白表达;半巢式甲基化特异性PCR法(MSP)和微流控芯片技术检测E- cadherin基因启动子甲基化状态.[结果]...     

Aspirin or Coumadin as the Drug of Choice for Valve Replacement with Porcine Bioprosthesis

Eight hundred twenty-four patients who had cardiac valve replacement with a porcine bioprosthesis were given either Coumadin (sodium warfarin) or aspirin. No patient in sinus rhythm no matter what valve or valves were replaced had thromboembolism whether treated with Coumadin (124 patients) or aspirin (260 patients). One hundred fifty-one patients who had mitral valve replacement, were in atrial fibrillation, and were treated with Coumadin had seven embolic events (4.6%), while 135 similar patients treated with aspirin had five embolic events (3.7%). Fifty-one patients who had double-valve replacement, were in atrial fibrillation, and were treated with Coumadin had three embolic episodes (5.9%), while 86 similar patients treated with aspirin had three embolic events (3.5%).No statistical difference was found in the incidence of thromboembolism between similar groups of patients whether treated with Coumadin or aspirin (p > 0.05). Hemorrhagic complications were higher and reached statistical significance (p < 0.001) for the group treated with Coumadin. This study shows that aspirin prevents thromboembolic complications as well as Coumadin in patients having cardiac valve replacement with a bioprosthesis, and results in a lower rate of complications.     

Inhibitory mechanisms of the hyper-irritability caused by picrotoxin in the rat

Topically applied norepinephrine, dopamine, serotonin, GABA and glycine, and systemically administered clonidine, l-DOPA (plus carbidopa) and 5-hydroxytryptophan completely suppressed the cutaneous hyper-irritability produced in the trigeminal sensory distribution by picrotoxin overlying the caudal medulla. Cholinergic agents and apomorphine were ineffective. Of the positive compounds, norepinephrine, serotonin and GABA showed the shortest latencies and norepinephrine and serotonin required the lowest concentrations in order to inhibit the hyper-irritability. If l-DOPA (plus carbidopa) was injected after pre-treatment with FLA-63, the effects of l-DOPA did not appear. Similar depression of the hyper-irritability was caused by electrical stimulation of the central gray. The inhibitory effects of stimulation of the central gray was suppressed after administration of tetrabenazine, but again produced markedly by injection of l-DOPA. From these observations it was concluded that the hyper-irritability could be suppressed by serotonergic as well as noradrenergic fibers terminating at the spinal trigeminal nucleus caudalis. The potential clinical use of l-DOPA in patients with hyperesthesia is discussed.     

An experimental model of hyper-irritability in the trigeminal skin field of the rat.

Cutaneous hyper-irritability was produced in the trigeminal sensory distribution of chronically prepared rats by injecting various central stimulants into the dorsal subarachnoid space overlying caudal medulla. Effective substances were classified into three groups according to the behavioral patterns induced. These were (1) picrotoxin and penicillin G-K, (2) strychnine and brucine, and (3) D,L-homocysteic acid. Of these substances, picrotoxin was the most effective. The hyper-irritability elicited by picrotoxin or penicillin G-K was associated with a relatively localized trigger zone in the face, while that elicited by strychnine or brucine spread beyond the trigeminal region to the first cervical dermatome. D,L-homocysteic acid evoked only spontaneous scratching and vocalization. Picrotoxin-induced hyper-irritability involved facilitation of impulse transmission from cutaneous afferent terminals to second-order neurons in the trigeminal nucleus caudalis.     

Immunoglobulin-producing cells in CSF and blood from patients with multiple sclerosis and other inflammatory neurological diseases enumerated by Protein-A plaque assay

Using the Protein-A plaque assay, numbers of IgG + IgA + IgM producing cells determined in patients with multiple sclerosis (MS) were 0.1–5% in CSF and 0.1–0.7% in peripheral blood; interestingly, 7 of 11 MS patients had IgM producing cells in CSF. In patients with aseptic meningitis (AM), the corresponding values were 0.04–7.5% in CSF and 0.4–2.4% in peripheral blood. There were more Ig producing cells in peripheral blood from patients with AM and MS than in healthy subjects. cocorrelation between numbers of IgG producing cells in CSF and the concentrations of intrathecally produced IgG (CSF IgG index) was registered in patients with AM: the same was true for IgA. The Protein-A plaque method, adopted for 20 × 10³ lymphocytes, makes possible enumeration of Ig-producing cells in CSF and discrimination among cells secreting different Ig classes, thereby being a powerful tool for studying immune reactions in the CNS-CSF compartment.