Cytokine levels in HIV infected and uninfected Indian women: Correlation with other STAs

Host immune status is an important determinant of disease progression. Infections in the genital tract may alter the immunity in the particular site and hence affect the production of local cytokines. We performed this study to determine whether HIV in association with cervical HPV and CT/GC infections influences the production of local cytokines. Cervical secretions from 100 women with or without HIV infection were collected for measuring IL-1β, -6, -10 and -12 concentrations by ELISA. Cervical HPV and CT/GC DNA were detected by HCII test. Significant elevations of IL-6 and IL-10 were observed in patients having HIV infection. Although cervical HPV infection increased the concentrations of both IL-6 and IL-1β but HPV induced abnormal cervical smear was associated only with increased IL-6 concentrations significantly. Double infection had marked relation with IL-6 and IL-10. CT/GC had no direct effect on any of these cytokines but in association with HIV and HPV, these bacterial pathogens elevated the concentrations of IL-6 significantly. Thus, our results suggest that the presence of HIV and other STAs in the genital tract can cause imbalance of local cytokine levels which in turn may facilitate other opportunistic infections.     

艾灸、中药对衰老模型大鼠线粒体DNA、血清IL-2、IL-6含量的影响

目的:探讨艾灸、中药延缓衰老的可能作用机制。方法:采用SD健康清洁级大鼠60只,随机分为青年对照组、衰老模型组、艾灸组、中药组、艾灸加中药组。后4组通过皮下注射D-半乳糖建立衰老大鼠模型,在造模过程中,3个治疗组分别给予艾灸(艾条温和灸"足三里""肾俞""关元")、中药(六味地黄汤加当归、丹参水煎液灌胃)、艾灸加中药治疗。治疗40 d后,测定各组大鼠肝细胞线粒体DNA含量及血清白介素2(IL-2)、白介素6(IL-6)含量。结果:①与青年对照组相比,衰老模型组大鼠肝细胞线粒体DNA和血清IL-6含量明显增加(P<0.05,P<0.01)、血清IL-2含量明显减少(P<0.05)。②与衰老模型组相比,各治疗组大鼠肝细胞线粒体DNA和血清IL-6含量明显减少(P<0.05,P<0.01)、血清IL-2含量明显增加(P<0.05)。结论:艾灸、中药可通过减少肝细胞线粒体DNA、血清IL-6含量,提高血清IL-2含量,从而起到延缓衰老的作用。     

白细胞介素-24抗肿瘤机制的研究进展

人类黑色素瘤分化相关基因-7/白介素-24(MDA-7/IL-24)是近年发现的、具有细胞因子样特性的肿瘤抑制基因。MDA-7/IL-24基因能抑制肿瘤生长、诱导细胞凋亡。     

Hypothalamo-pituitary-adrenal function in patients with depressive disorders is correlated with baseline cytokine levels, but not with cytokine responses to hydrocortisone

Dysfunction of the hyopthalamo-pituitary adrenal (HPA) system is frequently found in major depression. In addition, signs of non-specific inflammatory system activation have been reported. However, very little is known about interactions between the HPA and immune systems in depressive patients. To assess HPA system function, we performed a combined dexamethasone suppression and corticotropin-releasing hormone stimulation (DEX/CRH) test in 14 depressive patients. Moreover, baseline nocturnal plasma levels of the inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor (TNF)- were measured. In addition, the system was challenged with an intraveneous pulsatile injection of hydrocortisone (1 mg/kg body weight in total) and again cytokine levels were measured across one night. Baseline TNF- levels were negatively correlated with the amount of ACTH released upon CRH stimulation during the DEX/CRH test. Acute hydrocortisone administration suppressed TNF- and IL-6 levels independently of baseline HPA system activity. We conclude that chronic HPA system overactivity in depressed patients might compromise the production of inflammatory cytokines under baseline conditions. However, the responsivity of the cytokine production to acutely administered glucocorticoids does not seem to correlate with the state of the HPA system.     

外阴阴道假丝酵母菌病患者阴道人类防御素分泌及发病相关因素的研究

目的 通过对外阴阴道假丝酵母菌病(VVC)患者与健康妇女的比较,对VVC发病的相关因素进行分析,并研究VVC患者的阴道局部免疫状态.方法 应用病例对照研究方法,对VVC患者(VVC组,60例)及无VVC妇女(正常对照组,60例)进行比较,研究对象均填写调查表,取阴道分泌物进行阴道分泌物常规检查、pH值检测及细菌培养,用酶联免疫吸附试验对阴道冲洗液进行相关细胞因子[白细胞介素2、白细胞介素4、人类防御素5、人类β防御素(HBD)1、HBD2等]含量的检测分析.结果 (1)两组妇女的学历、对妇科感染的了解程度、妇科炎症病史、卫生习惯、性生活情况、药物应用情况比较,差异均无统计学意义(P>0.05);正常对照组慢性宫颈炎的发生率(43%,26/60)高于VVC组(22%,13/60),两组比较,差异有统计学意义(P<0.05).(2)正常对照组与VVC组妇女阴道pH值比较,差异无统计学意义(P>0.05).(3)VVC组妇女阴道分泌物检出假丝酵母菌43例,检出率为72%(43/60).(4)VVC组妇女阴道冲洗液中,人类防御素5、HBDI、HBD2含量[分别为(0.94±0.44)mg/L、(3.1±0.4)μg/L、(10±6)μg/L]明显高于正常对照组[分别为(0.61±0.27)mg/L、(2.7±0.4)μg/L、(7±3)μg/L],分别比较,差异均有统计学意义(P<0.05).结论 VVC是女性常见的外阴阴道炎症,在育龄期妇女VVC的发生与学历、对妇科感染的了解程度、妇科炎症病史、卫生习惯、性生活情况、药物应用情况无明显相关性.人类防御素的作用可能与VVC的发病密切相关.     

2型糖尿病患者生物心理因素的研究

目的探讨2型糖尿病患者有关的生物心理因素。方法对90例2型糖尿病患者和30名正常人进行明尼苏达多相人格调查表(MMPI)、多伦多述情障碍量表(TAS)、汉密尔顿抑郁量表(HAMD)、汉密尔顿焦虑量表(HAMA)、社会支持量表(SS)等评定,测定血浆促肾上腺皮质激素(ACTH)、血小板5-羟色胺(5-HT)、血清白介素(IL)6(IL-6)及SIL-2R水平,并检测患者空腹血糖(FBG)、早餐后2h血糖(2PBG)和糖化血红蛋白指数(HbA1c)水平。将患者随机分为常规治疗组(常规组,45例;采用格列吡嗪7.5-15 mg／d)和常规治疗加帕罗西汀治疗组(合用帕罗西汀组,45例;采用格列吡嗪7.5~15 mg／d和帕罗西汀20 mg／d),治疗2个月后(两组分别失访4例和9例)观察患者上述量表评分、实验室指标的变化及其与FBG、2PBG、HbA1c等指标变化的关系。结果(1)治疗前,与正常对照组比较,患者组TAS、HAMD、HAMA总分高,SS总分较低(P<0.05),MMPI提示人格偏移;血浆ACTH、血清IL-6、SIL-2R及FBG浓度高,血小板5-HT浓度低(均P<0.01和<0.05)。(2)治疗后,合用帕罗西汀组的HAMD分、HAMA分、ACTH、IL-6以及糖代谢指标的改善程度均优于常规组。(3)患者组的HAMD减分率与ACTH变化率(r=0.37)及IL-6变化率(r=0.40)均呈正相关,HAMA减分率与ACTH变化率(r=0.41)呈正相关(均P<0.01);FBG下降率与H     

Do repeated runs of a cell saver device increase the pro-inflammatory properties of washed blood?

OBJECTIVE: Intra-operative cell salvage is increasingly used, especially in longer cases with continuing blood loss. However it is unknown if the quality of processed blood is affected when larger quantities of blood are processed. We hypothesized that the quality of the washed blood decreases after multiple runs. METHODS: Intra-operative cell salvage was performed in 42 consecutive patients undergoing cardiac surgery. When 1250 ml of blood was collected in the blood collection reservoir, this was processed and returned to the patient. In 21 patients more than 2500 ml of blood was collected during the whole procedure, thus allowing at least two subsequent runs with the auto-transfusion device. Blood samples were drawn from the blood collection reservoir of the cell saver device before, and from the processed blood after each run. RESULTS: After the first run interleukin-6 concentrations were reduced with 85% (from 21+/-35 microg/l to 3.1+/-4.4 microg/l), whereas after the second run 72% was removed (63+/-69 microg/l to 17.6+/-25.3 microg/l). Leukocyte counts almost doubled after both processing runs (from 2.6+/-1.5 x 10(9)/l to 5+/-3.6 x 10(9)/l) and from 3.9+/-2.2 x 10(9)/l to 7.7+/-5.9 x 10(9)/l), hemoglobin concentration (14.8+/-1.6 mmol/l vs 15.0+/-1.1 mmol/l), free hemoglobin (2.3+/-1.6g/l vs 2.1+/-1.4 g/l) and platelet counts (18+/-9 x 10(9)/l vs 28+/-23 x 10(9)/l) were not different between the two runs. CONCLUSIONS: Our results suggest, based on interleukin-6 and free hemoglobin washout that the quality of the processed blood remains constant with multiple runs of the cell saver device.     

白介素家族在急性格林-巴利综合征发病机制中的作用

细胞因子在急性格林-巴利综合征(GBS)发病机制中所起的作用越来越重要,尤其是白介素(IL)在急性格林-巴利综合征发病过程中所起的促炎、抑炎作用越来越重要。近期研究表明IL-12、IL-17、IL-22在急性GBS的发病过程中起着重要的促炎作用,IL-35、IL-37起着重要的抑炎作用。本文对IL-17、IL-22、IL-35、IL-37、IL-9在急性GBS发病机制中所起的作用加以总结。     

白细胞介素-33的测定及其与早期类风湿关节炎相关性的研究

目的 通过检测早期类风湿关节炎(RA)患者血清中的白细胞介素(IL)-33水平,分析其与早期RA之间的相关性.方法 收集病程<1年的早期RA患者100例,骨关节炎(OA)患者40例以及健康对照者70名.采用双抗体夹心酶联免疫吸附试验(ELISA)测定血清中的IL-33水平,并分析血清IL-33水平与RA各临床和实验室指标的相关性.计量资料的比较采用Kruskal-Wallis检验和(或)Mann-Whitney U检验,计数资料比较采用X2检验,相关性分析采用Spearman相关分析.结果 RA患者血清IL-33水平为(282±871)pg/ml,显著高于健康对照组[(7±38)pg/ml,P(0.01)和OA患者[(8±35)pg/ml,P<0.01].血清IL-33水平与类风湿因子(RF)、隐性类风湿因子IgG(HRF-IgG)、抗环瓜氨酸肽(CCP)抗体、抗突变型瓜氨酸化波形蛋白(MCV)抗体呈正相关(r分别为:0.312,0.277,0.213,0.302,P<0.01或P<0.05). IL-33阳性组患者的RF阳性率、HRF-IgG阳性率、抗CCP抗体阳性率、抗MCV抗体阳性率(86%、31%、86%、94%)较IL-33阴性组患者(54%、11%、42%、72%)显著升高(P均<0.05). 结论 IL-33在RA患者血清中显著升高,并与多种自身抗体(包括RF、抗CCP抗体、抗MCV抗体和HRF-IgG)显著相关,可能是RA预后不良的因素之一.     

冠心病患者血浆白细胞介素10、18和C反应蛋白的变化及其意义

目的:探讨血浆白细胞介素(IL)-10,IL-18和C反应蛋白(CRP)在冠心病(CHD)发病过程中的作用及其临床意义。方法:采用酶联免疫双抗体夹心法(ELISA)对106例CHD患者[急性心肌梗塞(AMI)36例,不稳定型心绞痛(UAP)30例,稳定型心绞痛40例(SAP)]和32例正常人血浆中IL-10,IL-18和CRP的浓度进行检测。并根据Gensini积分,106例CHD患者又被分为A组(Gensini积分26分)、B组(Gensini积分26~54分)和C组(Gensini积分54分),对各组上述指标进行比较。结果:CHD患者的SAP组、UAP组、AMI组血浆IL-10水平依次为[(115.56±53.11)pg/ml、(86.00±30.55)pg/ml、(72.38±19.55)pg/ml],IL-18水平依次为[(217.53±95.83)pg/ml、(327.93±80.36)pg/ml、(430.28±71.62)pg/ml]和CRP水平依次为[(0.61±0.38)mg/L、(0.84±0.44)mg/L、(1.72±0.67)mg/L],均分别显著高于正常对照组[(49.58±19.82),(154.97±88.65),(0.46±0.34)mg/L,P均0.05]。冠心病C组血浆IL-18(389.21±93.55),CRP(0.86±0.48)水平明显高于B组[(331.32±106.59),(0.62±0.38)]及A组[(250.20±106.89),(0.42±0.34)];B组的显著高于A组的(P均0.05)。结论:虽然IL-10作为冠心病的保护因子水平升高,但不足以阻止冠心病的发生、发展,IL-18和CRP水平与冠心病的发生发展相关,其水平的检测对冠心病诊断及病情判断有重要意义。