IL-24基因与肿瘤免疫治疗

白细胞介素-24(IL-24)又称为黑色素瘤分化相关基因-7(MDA-7),它主要由CD3+ T淋巴细胞和单核细胞表达,为分泌蛋白,由206个氨基酸组成,属于IL-10家族成员。IL-24是第1个被报道的具有白介素作用的肿瘤抑制分子,具有选择性的广谱抗肿瘤活性。IL-24基因通过抑制血管形成、激活生长抑制和DNA损伤基因(GADD)等信号传导途径,抑制肿瘤细胞生长和促进肿瘤细胞凋亡,而对正常细胞无明显影响。INGN241是一种新开发的基因治疗制剂,对多种肿瘤具有抑制和治疗作用。     

急性肺损伤大鼠肺组织白细胞介素-13表达的初步研究

目的：动态观察急性肺损伤（ＡＬＩ）大鼠肺组织白细胞介素－１３（ＩＬ－１３）mＲＮ Ａ表达的变化,初步探讨ＩＬ－１３mＲＮＡ的表达。结果：正常肺组织表达一定水平的Ｉ Ｌ－１３mＲＮＡ;在ＡＬＩ大鼠肺组织,ＩＬ－１３mＲＮＡ表达呈短暂性上调,随即逐渐下调。结论：正常肺组织可表达一定水平的ＩＬ－１３mＲＮＡ;在ＡＬＩ肺组织,ＩＬ－ １３mＲＮＡ表达逐渐下调,提示局部抗炎作用减弱,可能引起抗炎和致炎作用失衡,从而     

白细胞介素类mRNA在儿童哮喘中异常表达的检测及意义

目的：研究白细胞介素－12（IL－12），IL－13在哮喘发病中的作用。方法：用逆转录聚合酶链反应（RT－PCR）法半定量分析了哮喘急性发作期及正常对照组儿童外周血单个核细胞（PBMC）中IL－12，IL－13mRNA表达水平的变化，同时对IgE水平进行了检测，结果：哮喘组IL－12 mRNA表达减少，IL－13 mRNA表达增多，病情越重，IL－12mRNA表达越少，IL－13 mRNA表达越多，无论IgE升高与否。与对照组比较，IL－12，IL－13mRNA表达，差异均有显著性，结论：IL－12，IL－13可能是构成气道慢性炎症的各类因素之一。     

不同缺血后处理对缺血再灌注大鼠脑内IL-1β、IL-6表达的影响

目的对局灶性缺血/再灌注大鼠实施不同缺血后处理,观察其对大脑炎症介质释放的影响,为其对缺血性脑损伤的保护提供依据。方法成年SD大鼠25只,采用线栓法制作大鼠大脑中动脉缺血/再灌注模型,随机分为5组,分别为假手术组、缺血再灌注组、缺血后处理组、远隔后处理组、延迟后处理组,术后48 h取脑组织,用免疫组化法观察缺血侧皮质和海马内IL-1β、IL-6表达及相同部位轴突形态。结果和缺血/再灌注组相比,缺血后处理和远隔后处理组损伤侧皮质和海马缺血范围减小,局部炎症反应减轻,轴突损伤减少。结论大鼠局灶性脑缺血/再灌注损伤后早期进行缺血后处理可显著减少大脑炎症反应,使轴突的损伤情况改善。     

188 Re-抗CEA单克隆抗体与白细胞介素12基因抗结肠癌协同效应

目的在非免疫缺陷荷结肠癌动物模型上观察^188Re-抗癌胚抗原（CEA）单克隆抗体（C50）与白细胞介素12（IL12）基因抑制肿瘤生长的协同作用：方法建立小鼠荷结肠癌动物模型，随机分组后分别施以化疗（CT）、^188Re-C50放射免疫治疗（RIT）、IL12基因治疗（GT）及RIT与IL12 GT联合治疗，治疗后3周比较上述各组及对照组（注射生理盐水）的抑瘤效果，同时采用酶联免疫吸附法检测GT、RIT及GT＋RIT3组血清IL12水平；并于治疗6h时采用流式细胞仪检测3组小鼠肿瘤不同周期细胞比率。结果不同治疗后3周各组肿瘤体积、质量比较示，RIT组与RIT＋GT组肿瘤体积和质量均明显低于CT组和对照组（P〈0．01），RIT、RIT＋GT两组间差异有显著性（P〈0．01）。血清IL12水平以RIT＋GT组最高，与GT和RIT两组相比差异有显著性（P〈0．01）。RIT、GT和RIT＋GT3组肿瘤S期细胞分别占10．41％、27．53％和6．25％，G0～G1期细胞分别占68．60％、53．54％和72．21％；而对照组分别为33．14％和35．12％。结论^188Re-C50与IL12基因联合可有效抑制肿瘤生长，疗效优于单独CT、RIT及IL12 GT：RIT＋GT可明显降低肿瘤S期细胞比率，并增加G0～G1期细胞比率；^188Re-C50可上调IL12基因表达。     

急性百草枯中毒血清细胞因子的动态变化

目的观察细胞因子白细胞介素(IL)-1β、IL-6、IL-10、肿瘤坏死因子-α(TNF-α)在急性百草枯中毒动物模型中的变化规律,探讨百草枯中毒急性肺损伤的作用机制。方法72只Wistar大鼠随机分为对照组及百草枯高剂量染毒组(120mg／kg)、中剂量染毒组(60mg／kg)、低剂量染毒组(30 mo／kg),分为8、24、72 h 3个观察时段,观察不同剂量组在染毒后不同时间大鼠静脉血细胞因子IL-1β、IL-6、IL-10、TNF-α的动态变化。结果8、24、72 h各剂量组细胞因子的水平均增加,24、72 h组与对照组比较,差异均有统计学意义(P<0．05或P<0．01)。72 h高剂量组各指标与同时间点低剂量组比较,差异有统计学意义(P<0．05)。72 h高、中、低3个剂量组与8 h时间点比较,差异有统计学意义(P<0．05)。随染毒浓度的增加,细胞因子水平逐渐增加。随时间的延长,细胞因子的水平也逐渐增加,存在一定的线性趋势,在整个实验过程中表现出动态的变化。结论在百草枯中毒急性肺损伤机制中细胞因子IL-1β、IL-6、IL-10、TNF-α可能起到了重要作用。     

Role of cytokines and growth factors in radioprotection

Cytokines and growth factors are growing groups of proteins that are responsible for the communication between cells of the immune system, hematopoietic cells, and other cell types. The cloning and large-scale production in a recombinant form of these agents in pharmacological quantities permitted investigations aimed at assessing the benefit they may provide in preserving and restoring functions of tissues compromised by irradiation. We have extensively examined past investigations which suggest that some cytokines and growth factors protect animals from radiation lethality when given prior to or after irradiation, and even in untreated animals, these cytokines serve in innate defenses against external stimuli. In contrast, some cytokines given before irradiation sensitize the animals to radiation lethality. Unfortunately, due to their adverse side effects, these cytokines were not found suitable as radioprotectors. Recent studies suggest that new approaches may bring cytokines and growth factors in clinic for radiation injury. The information and insight gained about therapeutic potential of cytokine manipulation will allow for more rational design of treatment protocols.     

Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas

OBJECTIVE: To determine the temporal and spatial expression of interleukins (IL)-13 and IL-15 in ovarian carcinoma compared to normal ovarian tissue. METHODS: Quantitative RT-PCR, ELISA and immunohistochemistry. RESULTS: Q-RT-PCR, ELISA and immunohistochemical analysis indicates that IL-13 and IL-15 mRNA and protein are expressed in normal ovary at various phases of the menstrual cycle with immunoreactive proteins detected in granulosa/theca and luteal cells and to a lesser extent in stromal cells and surface epithelial cells. Compared to normal ovary, ovarian carcinoma expresses elevated levels of IL-13 and IL-15 mRNA, with higher IL-13 expression in primary vs. metastatic tumors. IL-13 and IL-15 protein expression was also higher in the tumor tissues compared to ascites. In normal ovary, ovarian tumors and ascites, the ratio of IL-13/IL-15 favored IL-13. Immunoreactive IL-13 and IL-15 proteins were localized primarily in the tumor cells and infiltrated inflammatory cells with increased intensity with disease stage. CONCLUSION: Normal ovary and ovarian tumors express IL-13 and IL-15 and pattern of their expression in carcinomas suggests that these cytokines may function in various ovarian cellular activities including inflammatory/immune responses that are integrated cellular events taking place in normal ovary and ovarian tumors.     

Modifications in the production of cytokines and growth factors in drainage fluids following mesh implantation after incisional hernia repair

BACKGROUND: The aim of this study was to evaluate changes in the production of some cytokines (interleukins [ILs]-6, -10, -1, and -1ra), vascular endothelial growth factor, and beta-fibroblast growth factor after polypropylene mesh implantation. METHODS: Twenty female patients were divided into 2 groups. In 1 group, hernia repair was performed with conventional sutures (CR), whereas in the other group polypropylene mesh (MR) was used. Growth factors and cytokines production was analyzed in wound drain fluids based on the amount produced during 24 hours. RESULTS: IL-1 increased substantially in MR patients on postoperative days 1 and 2. IL1-ra and IL-10 production was always significantly higher in CR patients. IL-6 production did not show any considerable difference between the 2 groups. Vascular endothelial growth factor production was significantly higher in the MR than the CR group at all time points, whereas beta-fibroblast growth factor production was higher in the MR than the CR group only on postoperative day 1. COMMENTS: Our data suggest that different surgical procedures induce various levels of inflammation and that implantation of prostheses significantly stimulates the inflammatory response.