广东南海市登革热病原学及血清学检测

目的　为了明确广东省南海市１９９８ 年夏、秋季一批发烧、出疹病人的诊断。方法收集疑似登革热（ＤＦ）患者血清５２份,应用逆转录 聚合酶链反应（ＲＴ ＰＣＲ）、病毒分离和间接免疫荧光技术分别进行了病原学和血清学检测。结果　从２５ 份发病早期患者血标本中分离病毒１０ 份,经用单克隆抗体间接免疫荧光检测和ＲＴ ＰＣＲ检测证实ＤＥＮ２ 型感染。ＩｇＧ抗体检测,阳性率为４６－１５％ （２４／５２） ,最高滴度达１∶１２８０,ＩｇＭ 抗体检测阳性率为６０％ （１５／２５）。部分病人双份抗体检测,抗体滴度４ 倍升高。结论　此次南海市登革热暴发流行为登革２ 型感染所致。     

Active immunization against IL-23p19 improves experimental arthritis

Introduction

IL-23 is a pro-inflammatory cytokine essential for the differentiation of Th17 lymphocytes, a subtype of T lymphocyte implied in auto-immunity. IL-23 shares a subunit with IL-12, IL-12/23p40, and comprises a specific subunit, IL-23p19. We previously demonstrated that active immunization against entire TNF-α and against peptides of IL-1β was protective in animal models of rheumatoid arthritis. The aim of this study was to evaluate the effect of peptide-based vaccines targeting the IL-23p19 subunit in collagen-induced arthritis (CIA).

Methods

Using bioinformatics, the murine IL-23p19 subunit was modeled and two peptides were defined in the receptor interacting domain. Each peptide was coupled to keyhole limpet hemocyanin (KLH) to obtain two vaccines IL23-K1 and IL23-K2. Both vaccines were used for immunizations in incomplete Freund adjuvant (IFA) in groups of DBA/1 mice. Control groups received KLH or PBS at the same dates. CIA was induced by two subcutaneous injections of bovine type II collagen (CIIb), and the development of disease assessed during the next two months. Anti-CIIb and anti-IL-23 antibody levels were assessed by ELISA. Pro- and anti-inflammatory cytokines mRNA were quantified by qRT-PCR in the spleen and the synovium. T-cell populations in the spleen were evaluated by FACS analysis.

Results

The clinical scores showed that mice treated with IL23-K1 developed less arthritis than negative controls (p < 0.05). Mice immunized with IL23-K1 produced more anti-IL-23 antibodies than those immunized with IL23-K2 (p < 0.001). mRNA quantification showed that the IL23-K1 immunization led to an increase of IL-10 in the spleen (p < 0.05 vs KLH), without any effect on IL-17 level. Histological examination showed that IL23-K1 strongly protected against joint destruction and inflammation (p < 0.01 vs KLH and p < 0.001 vs PBS). T-cell populations in the spleen were not modified by IL-23 modulation.

Conclusion

These data show that targeting IL-23p19 through a vaccination strategy is protective in CIA. This specific targeting of IL-23 might constitute a promising therapeutic approach to explore in rheumatoid arthritis.     

吐鲁番地区维汉人群弓形虫病血清流行病学调查

本文采用间接荧光抗体试验(IFA)对新疆吐鲁番地区人群弓形虫病进行了调查研究,共检血清1165份,总阳性率为20.0％,维吾尔族阳性率为27.1％,汉族为14.5％,两者差异显著;两民族男女间比较差异无显著性。对IFA用于检测抗弓形虫抗体提出了看法;采用光学树脂—伊文思蓝来“划定抗原虫膜圈”。     

福建省宁化县斑点热血清学调查

目的：血清学调查人或动物感染斑点热情况。方法：微量补结室温结合法（ｍＣＦ）、间接免疫荧光法（ＩＦＡ）。结果：人群、野鼠、野猪、麂普遍感染，感染率分别为１８３５％、９４７％、５８３３％和１２５０％。结论：福建宁化地区可能存在斑点热群立克次体自然疫源地     

2004年大连市部分餐饮从业人员3种衣原体病原的血清抗体调查

[目的]了解大连市部分人群肺炎衣原体、沙眼衣原体和鹦鹉热衣原体感染情况。[方法］2004年采用免疫荧光（IFA）方法进行肺炎衣原体、沙眼衣原体、鹦鹉热衣原体抗体检测。[结果]肺炎衣原体、沙眼衣原体和鹦鹉热衣原体IgA的阳性率分别为51．69％（46／89）、20．22％（18／89）、3．37％（3／89）；肺炎衣原体、沙眼衣原体IgA阳性率在性别和年龄分布上差异无统计学意义（P〉0．05）。[结论]研究对象不同程度地存在3种衣原体的感染。     

间接免疫荧光法检测子宫内膜IgG抗体诊断子宫内膜异位症

目的：间接免疫荧光法测定子宫内膜IgG抗体,以探讨其诊断子宫内膜异位症（endometriosis,EMT)的临床价值。方法：共收集血清标本91份,分A（EMT）,B（正常对照）、C（疾病对照）三组,以EMT正位内膜冰冻切片作抗原片,用间接免疫荧光法检测各组血清中的子宫内膜IgG抗体。结果：A组荧光染色阳性率（87．2％）,与B、C组差异有显著性（P＜0．005）,荧光染色阳性率与EMT组的分期及月经周期的不同阶段无明显关系（P＞0．05）。用间接荧光法诊断EMT敏感性87．2％,特异性98．1％。阳性血清的几何均值为1：33．24（滴度范围1：20－1：320）。结论：用间接免疫荧光法检测血清中的子宫内膜IgG抗体诊断EMT敏感性强,特异性高。     

河南省莱姆病血清流行病学调查

为探明河南地区人群有否伯氏疏螺旋体（ｂｏｒｒｅｌｉａＢｕｒｇｄｏｒｆｅｒｉ）感染及感染程度，我们于１９９０～１９９３年，采用间接免疫荧光抗体试验，检测了本省不同地理环境的居民血清１９６３份，作了抗伯氏疏螺旋体抗体（ＩｇＧ）检测，检出抗体阳性１２４人，人群感染率为６．３２％，（新安县、罗山县、淮滨县分别为１３．８３％、５．８０％、３．９６％）；不同年龄组、职业、性别人群感染率无显著性差异。根据临床表现和血清流行病学调查，确诊莱姆病４０例，表明河南山区、平原地区人群均有不同程度感染。     

A comparison of antibody responses to veterinary vaccine antigens potentiated by different adjuvants

Six adjuvant formulations were compared for their ability to potentiate the primary and memory antibody responses in mice to three companion animal vaccine immunogens—feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), and a recombinantly-derived heartworm antigen. The combination of a novel bacterial immunostimulator, gliding bacterial adjuvant (GBA), either adsorbed onto an aluminum hydroxide gel (Rehydragel™ HPA), or emulsified with a vehicle of polyalcohol and detergent, elicited the strongest memory responses to both virus preparations. Both forms of aluminum hydroxide gels administered without GBA gave similar levels of adjuvant effects, on par with or greater than those generated by incomplete Freund's adjuvant (IFA). The Acemannan™ immunostimulant was not effective in increasing the responses to the virus antigens, but increased the primary response to the heart-worm antigen over tenfold from control levels. All preparations appeared to be well tolerated, with no detectable adverse reactions observed in any of the 250 mice used. The proven safety of aluminum hydroxide adjuvants and the apparent absence of adverse reactions seen with GBA make this vehicle/adjuvant formulation worthy of additional study.     

A seroepidemiological study of the risks of Q fever infection in Japanese veterinarians

The causative agent of Q fever, a widespread zoonotic disease, is the bacteria Coxiella burnetii. Although cases of Q fever have been documented in countries throughout the world, the prevalence of the disease in Japan is not yet known. Q fever is a demonstrated occupational hazard to those employed in zoological professions, but the risk to Japanese veterinarians has not yet been quantified. In order to evaluate the risk to Japanese veterinarians, we performed a serological survey using serum samples from 267 veterinarians. Two control groups consisting of 352 medical workers and 2003 healthy blood donors were also evaluated. The antibody titers of the serum samples were measured by indirect immunofluorescence assay (IFA) using phase II C. burnetii Nine Mile strain as the antigen. The positive rate of IgG antibody was 13.5% in the veterinarians, which was higher than in the blood donors (3.6%, p < 0.001) and medical workers (5.1%, p < 0.001). These findings suggest that Japanese veterinarians have a higher risk of infection by C. burnetii than other members of the Japanese population. An interesting finding of this study was that positive rates of IgG and IgM antibodies in the blood donor group were higher in younger individuals. The IgM antibody positive rate was the highest in females under 30 years old.     

在量子点探针介导下共聚焦显微镜检测肾癌组织HSP70的表达意义

本研究探讨新型纳米荧光材料量子点在免疫荧光分析法中的应用价值。我们分别利用量子点QD605或异硫氰酸荧光素(FITC)作为免疫荧光分析中的标记探针,通过激光扫描共聚焦显微镜观察肾细胞癌组织中HSP70的表达,并比较所得图像之间的成像差异性和成像稳定性。在所有的肾细胞癌组织样本中,量子点QD605标记的图像信号都要比常用的荧光染料FITC标记信号发光强度更高,更有特异性,在激光连续照射1 h下QD605标记的图像未有明显的荧光漂白现象,表现出极佳的发光稳定性。因此以新型荧光材料量子点为标记探针,用免疫荧光分析法检测病理组织切片中的蛋白标记物更有特异性,有潜力代替传统的有机荧光染料应用于生物大分子的荧光标记和光学成像。