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51.
Immunocytochemical localization of an imidazoline receptor protein in the central nervous system 总被引:9,自引:0,他引:9
Imidazoline (I) receptors have been implicated in the regulation of arterial blood pressure and behavior although their distribution in the central nervous system (CNS) remains in question. Presumptive I- receptor sites were detected in the rat central nervous system with a polyclonal antibody to an imidazoline receptor protein (IRP) with binding characteristics of the native receptor. IRP-like immunoreactivity (LI) was detected in neurons and glia by light and electron microscopy. Spinal cord: processes were heavily labeled in superficial laminae I and II of the dorsal horn, lateral-cervical and -spinal nuclei and sympathetic cell column. Medulla: label was concentrated in the area postrema, rostral, subpostremal and central subnuclei of nucleus tractus solitarii, spinal trigeminal nucleus caudalis, and inferior olivary subnuclei. Visceromotor neurons in the dorsal vagal and ambigual nuclei were surrounded by high concentrations of immunoreactive processes. In reticular formation, label was light, though predominant in the intermediate reticular zone and ventrolateral medulla. Pons: label was detected in the neuropil of the periventricular gray, concentrated in the dorsal– and external–lateral subnuclei of lateral parabrachial nucleus, and present intracellularly in the mesencephalic trigeminal nucleus. Midbrain: IRP-LI was most heavily concentrated in the interpeduncular nucleus, nuclei interfascicularis and rostral-linearis, the subcommissural organ, central gray, and in glia surrounding the cerebral aqueduct. Diencephalon: high densities were detected in the medial habenular nucleus, nucleus paraventricularis thalami, other midline-intralaminar thalamic nuclei, the supramammillary and mediobasal hypothalamic nuclei. In the median eminence, immunolabeled processes were restricted to the lamina interna and lateral subependymal zone. Telencephalon: IRP-LI was concentrated in the central amygdaloid nucleus, bed nucleus of stria terminalis and globus pallidus, followed by moderate labeling of the medial amygdaloid nucleus, amygdalostriatal zone and caudoputamen, the hilus of the dentate gyrus, and stratum lacunosum-moleculare of field CA1 of Ammon's horn. The subfornical organ and organum vasculosum lamina terminalis were filled with diffuse granular immunoreactivity. Ultrastructural studies identified IRP-LI within glia and neurons including presynaptic processes. I-receptor(s) localize to a highly restricted network of neurons in the CNS and circumventricular regions lying outside of the blood-brain barrier. Putative imidazoline receptors have a unique distribution pattern, show partial overlap with 2 adrenoreceptors and are heavily represented in sensory processing centers and the visceral nervous system. 相似文献
52.
The peripheral type benzodiazepine binding site (PTBBS) has been proposed to be a good marker for reactive glial cells following brain insults. In the present study, homogenate binding of 3H-Ro5-4864 and quantitative autoradiography of 3H-PK-11195 binding (two ligands for the PTBBS) were used to assess the distribution, time-course and extent of reactive gliosis in the hippocampus following deafferentation by unilateral entorhinal cortex lesion or neuronal death produced by intrahippocampal colchicine injection. Intrahippocampal colchicine injections produced a 3-fold increase in 3H-Ro5-4864 binding in the dentate gyrus within 2 days. This effect was doubled in animals pretreated with the lysosomal inhibitor chloroquine. Quantitative autoradiography of 3H-PK-11195 binding 1 or 2 weeks after colchicine injection indicated that the increase in binding was restricted to the dorsal hippocampus both rostrally and caudally and was present in the dentate gyrus and CA1. Following a unilateral electrolytic lesion of the entorhinal cortex, the binding of 3H-Ro5-4864 to homogenates of the dentate gyrus was doubled 18 h after the lesion, reached a maximum at 4 days post-lesion, and returned to control values by 2 months after the lesion. A transient increase in binding was also observed 2 and 4 days post-lesion in the dentate gyrus contralateral to the lesion side. Autoradiography of 3H-PK-11195 binding indicated that the increase in PTBBS following entorhinal cortex lesion was restricted to the molecular layer of the dentate gyrus.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
53.
V. T. F. Yeung S. K. S. Ho D. H. Y. Leung A. Stadlin M. G. Nicholls C. S. Cockram 《Glia》1993,9(4):243-247
We prepared primary cultures of mouse astrocytes from the cerebral cortex, hypothalamus, and cerebellum to examine the possibility of regional disparity in binding of human atrial and porcine brain natriuretic peptides (hANP, pBNP) and their effect on cyclic guanosine monophosphate (cGMP) production. 125I-hANP and 125I-pBNP bound in a specific and saturable manner to all three regions. For both peptides, Scatchard analysis suggested a single population of binding sites on astrocytes from all three regions. No significant differences were observed in the maximal binding capacities (Bmax) or binding dissociation constants (KD) between the two peptides in the astrocyte preparations from different regions. ANP and BNP also evoked cGMP stimulation in a similar, dose-dependent fashion in astrocytes from all three regions, with maximal responses to both peptides reached at a concentration above 1 μM. While BNP elicited a greater maximal cGMP accumulation than ANP, no difference could be demonstrated in the cGMP responses to either peptide between brain regions. Thus we have been unable to demonstrate regional heterogeneity in the responsiveness of astrocytes to ANP and BNP. © 1993 Wiley-Liss, Inc. 相似文献
54.
Astrocytes are the most abundant cell type in the mammalian central nervous system (CNS), and are involved in many processes critical for normal CNS maintenance and function. We have used double-label immunocytochemistry and in situ analysis to show that the SPARC (secreted protein acidic and rich in cysteine)-related protein SC1, co-localizes with the astrocyte marker glial fibrillary acidic protein (GFAP) in the adult rodent brain. Thus, SC1 is an astrocyte marker that may be used to investigate astrocyte heterogeneity and analyze glial cell lineages during neural development. Consistent with the presence of SC1 and GFAP in astrocytes, both proteins were markedly upregulated following reactive astrocytosis induced by focal mechanical trauma. Therefore, SC1 may play an important role in reactive astrocytosis subsequent to a wide variety of neural trauma, including neurodegenerative diseases and acute neural damage. 相似文献
55.
目的研究血液内较高浓度的可溶性β-淀粉样蛋白1-40(Aβ1-40)对毛细血管内皮细胞和胶质细胞的毒性,以探讨其在阿尔茨海默病发病中的作用.方法成年雄性SD大鼠90只,随机分为注射Aβ1-40组、注射生理盐水组以及正常大鼠(非手术)组,每组30只.采用右侧颈外静脉血管插管并留置的方法,通过导管向血管内中每日2次注射10μg的可溶性的Aβ1-40,连续14天后,采用免疫组化和免疫印迹(Western blot)方法观察星形胶质细胞、小胶质细胞的激活状态以及转糖蛋白-1、转糖蛋白-3表达的改变.结果注射Aβ1-40以后,毛细血管周围星形胶质细胞的胶质纤维酸性蛋白表达明显增多;小胶质细胞呈明显的激活状态;Western blot法检测到脑实质内转糖蛋白-1以及转糖蛋白-3表达明显减少.结论血管内连续注射Aβ1-40以后,对血-脑屏障的内皮细胞有明显的损害,对胶质细胞有明显的激活作用,可能与AD发病有关. 相似文献
56.
G. M. Lauro C. Fabrizi R. Businaro L. Fumagalli S. Torelli F. Gremo 《The Italian Journal of Neurological Sciences》1992,13(8):661-665
Alpha2-macroglobulin (α2
M) is a serum proteinase inhibitor with a broad specificity. At present its role in human brain is unknown, but recent data
report its presence in the CNS, particularly at glial level.
Previous studies from our group demonstrated the synthesis and secretion of α2
M in different glial cultures derived from an astrocytoma and a glioblastoma. In the present study a human fetal astroglial
cell line and two microglial established cell lines are examined for the presence of α2
M by using polyclonal antibodies in ELISA and immunofluorescence assays. While we observed a strong specific positivity in
the cytoplasm and in the culture medium of the GFAP, vimentine positive cells, no positivity was detected in FcR, Iysozyme
positive microglial cells.
Since interaction of proteinases and proteinase inhibitors appear to play a crucial role in the development of neuroimmunological
compentence, these data suggest a dissociation of macro and micro-glia immune functions.
Paper presented at the National Congress at Sorrento in 1991 and selected by the Editorial Board of the Journal 相似文献
57.
Zsuzsanna Huszti H. Prast Manh Hung Tran Hanspeter Fischer Athineos Philippu 《Naunyn-Schmiedeberg's archives of pharmacology》1997,357(1):49-53
The ability of glial cells to take up histamine in vitro suggests that these cells may be involved in histamine inactivation.
This prompted us to study the possible interactions between neuronal and glial processes which determine the histamine concentration
in the synaptic cleft. In vitro experiments showed that the glial metabolic toxin, fluoroacetate (20 and 40mmol/l) depressed
histamine uptake into cultured astroglial cells and dissociated hypothalamic cells of rats. For in vivo experiments, the push-pull
superfusion technique was used. In anaesthetized rat, the anterior hypothalamic area was superfused through the push-pull
cannula with artificial cerebrospinal fluid (aCSF) or with aCSF which contained fluoroacetate and the release of endogenous
histamine was determined in the superfusate.
Hypothalamic superfusion with fluoroacetate (20mmol/l) led to a pronounced increase in extracellular histamine. The effect
of fluoroacetate was inhibited by 5μmol/l tetrodotoxin. Superfusion with Ca++-free, Mg++-rich (12mmol/l) aCSF inhibited the basal release rate of histamine. Under these conditions, 20mmol/l fluoroacetate did not
modify the level of the amine in the superfusate.
These data demonstrate that depression of glial function enhances the concentration of histamine in the extracellular space
by slowing down the uptake of the amine into the glial cells. Thus, under in vivo conditions, glial cells are directly involved
in the continuous removal of neuronal histamine from the synaptic cleft. 相似文献
58.
Heidrun Kuhrt Antje Wurm Anett Karl Ianors Iandiev Peter Wiedemann Andreas Reichenbach Andreas Bringmann Thomas Pannicke 《International journal of developmental neuroscience》2008
A decrease in the expression of inwardly rectifying potassium (Kir) currents is a characteristic feature of retinal glial (Müller) cells in various retinopathies, e.g., after transient retinal ischemia. We used short-term retinal organ cultures to investigate whether similar physiological alterations can be induced under in vitro conditions. During 4 days in vitro, Müller cells displayed a decrease in Kir currents and an increase in transient A-type potassium currents which was similar to the alterations in membrane physiology during ischemia-reperfusion in vivo. In addition, gliosis of Müller cells both in vivo and in organ cultures was associated with cellular hypertrophy and an alteration in osmotic swelling characteristics. Whereas Müller cells in control retinae did not swell under hypotonic stress, cells in postischemic retinae and in organ cultures swelled upon hypotonic challenge. Therefore, Müller cells in organ cultures can be used to investigate distinct aspects of ischemia-induced Müller cell gliosis. Both the decrease in Kir currents and the alteration in osmotic swelling may reflect a dysfunction of Müller cells regarding the control of the ionic and osmotic homeostasis in the retina. 相似文献
59.
Electrophysiological properties of glial cells: comparison of brain slices with primary cultures 总被引:2,自引:0,他引:2
Intracellular recordings were obtained from glial cells in the CA1 region of rat hippocampal slices to compare their electrophysiological properties with the previously reported properties of glial cells in primary tissue culture. The average resting potential was -77 mV and the average input resistance was 3.2 M omega. Barium (10 mM) depolarized glial cells in brain slices and increased input resistance, but barium action potentials which have been observed in primary cultures, were not observed in brain slices. gamma-aminobutyric acid (GABA) and glutamate depolarized glial cells. Spontaneous oscillations of membrane potential were observed occasionally. The mechanism underlying these responses are unknown as yet. 相似文献
60.
Transplantation of glial cells into demyelinating lesions in CNS offers an experimental approach which allows investigation of the complex interactions that occur between CNS glia, Schwann cells, and axons during remyelination and repair. Earlier studies have shown that (1) transplanted astrocytes are able to prevent Schwann cells from participating in CNS remyelination, but that they are only able to do so with the cooperation of cells of the oligodendrocyte lineage, and (2) transplanted mouse oligodendrocytes can remyelinate rat axons provided their rejection is controlled by immunosuppression. On the basis of these observations, we have been able to prevent the Schwann cell remyelination that normally follows ethidium bromide demyelination in the rat spinal cord by co-transplanting isogeneic astrocytes with a potentially rejectable population of mouse oligodendrocyte lineage cells. Since male mouse cells were used it was possible to demonstrate their presence in immunosuppressed recipients using a mouse Y-chromosome probe by in situ hydridisation. When myelinating mouse cells were rejected by removal of immunosuppression, the demyelinated axons were remyelinated by host oligodendrocytes rather than Schwann cells, whose entry was prevented by the persistence of the transplanted isogeneic astrocytes. The oligodendrocyte remyelination was extensive and rapid, indicating that the inflammation associated with cell rejection did not impede repair. If this host oligodendrocyte remyelination was prevented by local X-irradiation, the lesion consisted of demyelinated axons surrounded by processes from the transplanted astrocytes. By this approach, it was possible to create an environment which resembled the chronic plaques of multiple sclerosis. Thus, these experiments demonstrate that in appropriate circumstances the temporary presence of a population of glial cells can alter the outcome of damage to the CNS. © 1995 Wiley-Liss, Inc. 相似文献