全文获取类型
收费全文 | 1644篇 |
免费 | 94篇 |
国内免费 | 45篇 |
专业分类
耳鼻咽喉 | 48篇 |
儿科学 | 17篇 |
妇产科学 | 16篇 |
基础医学 | 377篇 |
口腔科学 | 42篇 |
临床医学 | 44篇 |
内科学 | 275篇 |
皮肤病学 | 36篇 |
神经病学 | 331篇 |
特种医学 | 17篇 |
外科学 | 102篇 |
综合类 | 96篇 |
预防医学 | 48篇 |
眼科学 | 65篇 |
药学 | 183篇 |
中国医学 | 18篇 |
肿瘤学 | 68篇 |
出版年
2023年 | 7篇 |
2022年 | 32篇 |
2021年 | 45篇 |
2020年 | 22篇 |
2019年 | 28篇 |
2018年 | 32篇 |
2017年 | 40篇 |
2016年 | 41篇 |
2015年 | 50篇 |
2014年 | 70篇 |
2013年 | 96篇 |
2012年 | 58篇 |
2011年 | 72篇 |
2010年 | 71篇 |
2009年 | 93篇 |
2008年 | 96篇 |
2007年 | 91篇 |
2006年 | 83篇 |
2005年 | 67篇 |
2004年 | 55篇 |
2003年 | 72篇 |
2002年 | 55篇 |
2001年 | 52篇 |
2000年 | 43篇 |
1999年 | 32篇 |
1998年 | 39篇 |
1997年 | 33篇 |
1996年 | 30篇 |
1995年 | 24篇 |
1994年 | 17篇 |
1993年 | 24篇 |
1992年 | 20篇 |
1991年 | 11篇 |
1990年 | 20篇 |
1989年 | 12篇 |
1988年 | 13篇 |
1987年 | 15篇 |
1986年 | 8篇 |
1985年 | 9篇 |
1984年 | 11篇 |
1983年 | 14篇 |
1982年 | 16篇 |
1981年 | 6篇 |
1980年 | 20篇 |
1979年 | 12篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1973年 | 5篇 |
1969年 | 1篇 |
排序方式: 共有1783条查询结果,搜索用时 0 毫秒
51.
目的 通过观察血吸虫可溶性虫卵抗原(SEA)孵育培养的脑血管内皮细胞缝隙连接(GJ)蛋白的表达和分布,探讨脑动脉GJ在脑血吸虫病病理发生中的作用。方法 使用血吸虫SEA孵育培养幼兔脑基底动脉血管内皮细胞,实验分对照组、SEA 1~5组:加入SEA(质量浓度分别为10.0%、5.0%、3.3%、2.5%、2.0%),应用逆转录—聚合酶链式反应(RT-PCR)技术测定兔脑血管内皮细胞GJ蛋白Cx37 mRNA的表达;应用Western 印迹技术测定兔脑血管内皮细胞GJ蛋白Cx37蛋白的表达。结果 对照组和SEA 1 ~5组的GJ蛋白Cx37 mRNA水平分别为0.239±0.037、0.260±0.043、0.218±0.310、0.647±0.040、0.419±0.036、0.513±0.038;其中SEA 3~5组的GJ蛋白Cx37 mRNA水平高于对照组(P均<0.05)。对照组和SEA 1~5组GJ 蛋白Cx37蛋白表达分别为0.401±0.045、0.485±0.048、0.749±0.052、1.119±0.063、1.015±0.057、0.605±0.047,其中SEA 2 ~5组Cx37蛋白表达高于对照组(P均<0.05)。结论 血吸虫SEA孵育培养的兔脑血管内皮细胞GJ蛋白Cx37 mRNA及其蛋白水平高于对照组,提示GJ蛋白在SEA及其分泌物浸润脑动脉沉积于脑组织,从而在诱发脑血吸虫病的病理发生机制中可能起重要的作用。 相似文献
52.
目的通过比较有无音乐训练经历的正常听力青年人的时域分析能力,探讨音乐训练对听觉功能的影响。方法选择双耳平均听阈≤20 d B HL,无耳科疾病的290名青年人作为研究对象,其中有多年专业音乐训练背景的受试者为音乐训练组(130例,平均24.2岁),其余为对照组(160例,平均21.3岁),分别进行时域间隔感知能力测试,记录受试者间隙检测阈值,并使用SPSS 20.0进行数据分析。结果音乐训练组受试者间隙检测阈值为(3.44±1.42)ms;对照组间隙检测阈值为(4.45±1.39)ms,两组数据差异具有统计学意义(P0.05)。性别、耳别等因素对对照组的间隙检测阈值无明显影响(P0.05)。可听度对间隙检测阈值有影响(P0.05),当可听度20 d B SL时,随给声强度增加间隙检测阈值明显下降;当可听度20 d B SL时,随声强度增加间隙检测阈值无显著变化。结论听觉中枢时域处理能力与性别和耳别无关,受可听度影响,且音乐训练经历能明显提高时域处理能力。 相似文献
53.
Camillo Peracchia Giovanni Bernardini Lillian L. Peracchia 《Pflügers Archiv : European journal of physiology》1983,399(2):152-154
The cell-to-cell channels of gap junctions mediate the direct exchange of ions and small metabolites between neighboring cells. A number of studies have shown that these channels close when the intracellular free calcium or hydrogen concentration increases, the result being cell-to-cell uncoupling. Since most of the calcium-activated biological phenomena are mediated by calmodulin (CaM), an obvious question is whether or not CaM is involved in the mechanism of cell coupling regulation. Data from the present study, showing the inhibitory effects of a calmodulin blocker on electrical uncoupling in Xenopus embryo cells, suggest a possible CaM participation in the uncoupling mechanism. 相似文献
54.
连接蛋白拟似肽对海人酸致痫大鼠脑电活动的影响 总被引:1,自引:0,他引:1
目的观察针对连接蛋白43(CX43)合成的特异性的缝隙连接阻断剂-连接蛋白拟似肽对海人酸致痫大鼠脑电活动的影响。方法建立18只大鼠癫痫动物模型,分连接蛋白拟似肽组、甘珀酸组和对照组(每组6只),在在体上分别局部给予连接蛋白似似肽、甘珀酸和生理盐水,用脑电图仪观测用药前后每组大鼠皮层脑电活动的变化情况。结果连接蛋白拟似肽组及甘珀酸组给药后癫痫的发作次数明显比给药前发作次数减少,癫痫波的平均振幅也明显变小,给药前后比较有显著性差异(P<0.01),生理盐水组给药前后癫痫的发作次数及平均振幅几乎没有变化。连接蛋白拟似肽组给药前后癫痫的发作次数和波幅的变化值与甘珀酸组给药前后癫痫的发作次数和波幅的变化值相比无显著性差异。结论针对CX43合成的连接蛋白拟似肽可以特异性地抑制癫痫的发作。 相似文献
55.
We recently showed that Mts1 is expressed in white matter astrocytes in the rat brain and spinal cord from the first postnatal day. Its expression level declined in the adult CNS, but its topographical localization was maintained. Only white matter astrocytes in the cerebellum did not express Mts1. After dorsal root or sciatic nerve injury, we observed a marked upregulation of Mts1 in the area of the dorsal funiculus undergoing Wallerian degeneration. Here we show that upregulation of Mts1 is a consistent feature of astrocytes in white matter undergoing Wallerian degeneration. In addition, Mts1 is upregulated in astrocytes outlining the lesion site of a penetrating injury to the forebrain, or cerebellum. Gray matter astrocytes did not express Mts1, even after direct injury. In injured brain, we consistently noted a close relationship between Mts1-expressing astrocytes and ED1-positive microglia/macrophages, which are known to be highly motile cells. Mts1 was expressed in the periventricular area and the rostral migratory stream, i.e., sites of ongoing neuroplasticity in adulthood, and was upregulated in these areas after injury. These data suggest that Mts1-expressing astrocytes play a significant role in degenerative events in the mature white matter, interact with phagocytic microglia/macrophages and regulate cell migration and differentiation in areas of the adult brain with a high degree of plasticity. 相似文献
56.
Connexin26 (Cx26, GJB2) mutations can induce congenital deafness and are responsible for ∼50% of nonsyndromic hearing loss in children. Mouse models show that Cx26 deficiency induces cochlear development disorder, hair cell loss, and spiral ganglion (SG) neuron degeneration. Hair cell loss and cell degeneration have been considered as a primary causer responsible for Cx26 deficiency associated hearing loss. In this study, by coincidental examination of cochlear postnatal development with recording of auditory brainstem response (ABR) and hair cell function, we found that occurrence of hearing loss in Cx26 knockout (KO) mice was ahead of hair cell loss and cochlear cell degeneration. ABR was absent at the whole-frequency range (8–40 kHz) after birth. However, cochlear cells including SG neurons had no significant degeneration throughout postnatal development. Severe cochlear hair cell loss and SG neuron degeneration were only visible in middle and basal turns, i.e., in middle and high frequency regions, in the adult Cx26 KO mouse cochlea. Functional tests show that hair cells in Cx26 KO mice functioned normally; outer hair cells retained electromotility. These data suggest that cell degeneration is not a primary causer of Cx26 deficiency associated hearing loss. Some mechanisms other than cell degeneration, such as cochlear development disorders, may play an essential role in this common hereditary deafness. 相似文献
57.
Plaisance I Duthe F Sarrouilhe D Hervé JC 《Pflügers Archiv : European journal of physiology》2003,447(2):181-194
In cardiac myocytes of new-born rats, the degree of intercellular communication through gap junctional channels closely depends on the metabolic state of the cells. In contrast, in stably transfected HeLa cells expressing rat cardiac connexin43 (Cx43, the main channel-forming protein present in ventricular myocytes), a major part of junctional communication persisted in ATP-depleted conditions, in the presence of a metabolic inhibitor (KCN) or of a broad spectrum inhibitor of protein kinases (H7). However, another metabolic inhibitor, antimycin A, which like cyanide inhibits electron transfer in the respiratory chain, totally interrupted cell-to-cell communication between Cx43-HeLa cells, even in whole-cell conditions, when ATP (5 mM) was present. Antimycin A caused a modest increase in cytosolic calcium concentration; however, junctional uncoupling still occurred when this rise was prevented. Conditions of ischemic insult (e.g. ischemia or chemical hypoxia) frequently cause the activation of protein kinases, particularly of Src and MAP kinases, and such activations are known to markedly disrupt gap junctional communication. Antimycin-induced junctional uncoupling occurred even in the presence of inhibitors of these kinases. Antimycin A appears able to cause junctional uncoupling either through the ATP depletion it induces as a metabolic poison or via a direct action on gap junction constituents. 相似文献
58.
Perception of a phoneme may occur even when the speech sound is missing (e.g., when an extraneous noise replaces the sound). This phenomenon, called phonemic restoration, has been observed to depend on the type of distortion. It requires a replacing sound that provides acoustic input to the auditory system, since the restoration has not been found when a speech sound was replaced by silence. We examined the brain activation underlying speech processing when the word's initial phoneme was completely replaced by a silent gap. Event-related potentials (ERPs) and reaction times (RTs) were measured as indicators of semantic processing of sentence final words. Slower voice onset times during repetition of the manipulated words as compared to normal words indicated increased difficulty in retrieving their meaning. The N400, which is related to the increased demands of the semantic integration of words, was elicited by less expected words as compared to highly expected ones. For manipulated words, the N400 was elicited at the same latency than for normal words, with respect to the onset of the remaining word fragment. The amplitude of the N400 was not increased, nor did it last longer, thereby indicating successful retrieval of the word's meaning based on the semantic context and remaining phonetic information. Thus, semantic retrieval does not seem to require the word's initial phoneme to be present in a sentence context. The results suggest that both context-driven expectancy (top–down) and stimulus-driven processes (bottom–up) are utilized in word processing and contribute to the overall N400 response. 相似文献
59.
The brainstem nucleus hypoglossus innervates the tongue which must contract rhythmically during respiration, chewing and swallowing. Such rhythmic discharges are due to network bursting mediated by AMPA receptor-dependent glutamatergic transmission. The contribution by hypoglossal motoneurons themselves to rhythmicity remains, however, unclear as they might simply express cyclic patterns produced by premotoneurons or, in analogy to spinal motoneurons, might participate to bursting due to activation of their N-methyl-D-aspartate (NMDA) receptors. Using patch clamp recording from hypoglossal motoneurons in slice preparations of neonatal rat brainstem, we observed that NMDA directly depolarized motoneurons to generate various discharge patterns. Most motoneurons produced transient bursts which were consistently restored by repolarizing membrane potential to rest. Fewer motoneurons generated either sustained bursting or random firing. Rhythmic bursts were recorded from XII nerve rootlets even when single motoneuron bursting required hyperpolarization. NMDA evoked bursts were blocked by the Ca2+ antagonist Cd2+, the gap junction blocker carbenoxolone, or Mg2+ free solution, and partially inhibited by tetrodotoxin or nifedipine. Under voltage clamp, NMDA-induced bursting persisted at negative or positive potentials and was resistant to high extracellular Mg2+ in accordance with the observation of widespread motoneuron expression of NMDA 2D receptor subunits that confer poor Mg2+ sensitivity. It is proposed that NMDA depolarized motoneurons with the contribution of Mg2+ insensitive channels, and triggered bursting via cyclic activation/deactivation of voltage-dependent Na+, Ca2+ and K+ currents spread through gap junctions. The NMDA-evoked bursting pattern was similar to the rhythmic discharges previously recorded from the XII nerve during milk sucking by neonatal rats. 相似文献
60.
Genetic ablation of carbonic anhydrase IX disrupts gastric barrier function via claudin‐18 downregulation and acid backflux 下载免费PDF全文
A. K. Singh K. A. Mäkelä A. Seidler Y. Liu G. Gros H. Bartels K. H. Herzig U. Seidler 《Acta physiologica (Oxford, England)》2018,222(4)