The function of natural-killer (NK) cells is modulated by the balance between a number of activating and inhibitory receptors.
Killer immunoglobulinlike receptors (KIRs) are mostly inhibitory receptors. They play a critical role in recognizing self-class-I
major histocompatibility complex (MHC) molecules and thus protect healthy host cells from NK-targeted lysis. In contrast,
both NKG2D and CD16 are activating NK receptors that trigger the NK-cell lysis of various tumor and virally infected cells
through either direct ligand engagement or antibody-dependent cellular cytotoxicity (ADCC). Through structural studies of
members of these distinct receptor families, in particular, the structure and recognition between KIR2DL2 and HLA-Cw3, that
between NKG2D and ULBP3, and that between CD16 and IgG Fc, considerable understandings have been achieved about their function
and their ligand recognition. 相似文献
Rice (Oryza sativa) has become an important model plant species in numerous research projects involving genome, molecular and evolutionary biology.
In this review we describe the reasons why rice provides an excellent model system for centromere and heterochromatin research.
In most multicellular eukaryotes, centromeres and heterochromatic domains contain long arrays of repetitive DNA elements that
are recalcitrant to DNA sequencing. In contrast, three rice centromeres and the majority of the cytologically defined heterochromatin
in the rice genome have been sequenced to high quality, providing an unparalleled resource compared to other model multicellular
eukaryotes. Most importantly, active genes have been discovered in the functional domains of several rice centromeres. The
centromeric genes and sequence resources provide an unprecedented opportunity to study function and evolution of centromeres
and centromere-associated genes. 相似文献
We discuss the advantages and problems associated with fitting geometric data of the human torso obtained from magnetic resonance
imaging, with high-order (bicubic Hermite) surface elements. These elements preserve derivative (C1) continuity across element boundaries and permit smooth anatomically accurate surfaces to be obtained with relatively few
elements. These elements are fitted to the data with a new nonlinear fitting procedure that minimizes the error in the fit
while maintainingC1 continuity with nonlinear constraints. Nonlinear Sobelov smoothing is also incorporated into this fitting scheme. The structures
fitted along with their corresponding root meansquared error, number of elements used, and number of degrees of freedom (df)
per variable are: epicardium (0.91 mm, 40 elements, 142 df), left lung (1.66 mm, 80 elements, 309 df), right lung (1.69 mm,
80 elements, 309 df), skeletal muscle surface (1.67 mm, 264 elements, 1,010 df), fat layer (1.79 mm, 264 elements, 1,010 df),
and the skin layer (1.43 mm, 264 elements, 1,010 df). The fitted surfaces are assembled into a combined finite element/boundary
element model of the torso in which the exterior surfaces of the heart and lungs are modeled with two-dimensional boundary
elements and the layers of the skeletal muscle, fat, and skin are modeled with finite elements. The skeletal muscle and fat
layers are modeled with bicubic Hermite linear elements and are obtained by joining the adjacent surface elements for each
layer. Applications for the torso model include the forward and inverse problems of electrocardiography, defibrillation studies,
radiation dosage studies, and heat transfer studies. 相似文献
A series of poly(ether ester)s containing different H‐bonding units (amide, carbamate, urea) was prepared by polycondensation in bulk, using Ti(OBu)4 as a catalyst. The copolymers were obtained starting from PEG1000, 1,4‐butanediol, and a symmetrical, bis‐ester terminated monomer carrying H‐bonding units. These materials were designed for biomedical applications, in which ultimate biodegradability of the materials is required. The influence of the nature of the H‐bonding unit and of the length of the methylene spacer between H‐bonding groups on the thermal and solubility properties of copolymers was investigated. Amide containing copolymers were more thermally stable than ones containing carbamate, consistent with the observed behavior of the corresponding monomers. In most cases, differential scanning calorimetry (DSC) traces were quite complex because of phase separation and dependent on the applied cooling rate. Copolymers containing urea bonds were less soluble in most organic solvents, but their thermal properties were not significantly different than their amide containing counterparts.
In this study we used a novel technique to reveal both longitudinal and transverse differentiation within mammalian mitotic
chromosomes. Structural changes in chromosomes that we term ‘differential decondensation’ were produced in cells that were
first incubated in hypotonic medium (15% Hanks’ solution), then adapted to normotonic conditions and thereafter exposed to
a second short hypotonic shock. Such a double hypotonic treatment (DHT) is not critical for cell viability, but considerably
elongates the G2 phase of the cell cycle. Giemsa staining of differentially decondensed chromosomes corresponds to standard
G-banding, but does not need the standard post-fixation treatment. Using ‘dynamic’ BrdU banding, we show that such ‘differential’
staining is a result of differential resistance of the R- and G-bands to DHT. Thus, early-replicating foci, markers of R-bands,
are localized in the peripheral chromatin halo, whereas late-replicating foci, corresponding to G-bands, remain associated
with the axial regions of chromatids. Remarkably, despite these major changes in the structure of the chromosomal bands, the
replication foci still preserve their discrete structure. 相似文献