DS-1226对慢性睡眠干扰所致小鼠抑郁行为的改善作用

目的 研究人参皂苷水解产物DS-1226对慢性睡眠干扰小鼠的抗抑郁作用,为抗抑郁药物的研发提供科学依据。方法 将72只雄性ICR小鼠分为空白对照组、模型组、阳性对照组（盐酸帕罗西汀,10 mg/kg）、DS-1226低剂量组（20 mg/kg）、中剂量组（40 mg/kg）、高剂量组（80 mg/kg）。除空白组外,其他组小鼠先进行3 d的滚筒适应,然后连续进行14 d睡眠干扰。采用体重监测、自主活动实验、悬尾实验、强迫游泳实验等实验方法,评价DS-1226的抗抑郁作用。结果 连续进行14 d睡眠干扰后,与空白对照组相比,模型组体重显著下降,悬尾和强迫游泳不动时间显著增长。与模型组相比,DS-1226中剂量组显著逆转睡眠干扰所致的体重下降,其他各给药组均未能显著逆转睡眠干扰所致的体重下降;阳性药组的悬尾不动时间极显著减少,强迫游泳不动时间有减少趋势;DS-1226中剂量悬尾不动时间显著减少,强迫游泳不动时间有明显减少趋势;DS-1226高剂量组悬尾和强迫游泳不动时间均显著减少。结论 DS-1226具有改善慢性睡眠干扰小鼠抑郁样行为的作用。     

脑蛋白水解物片剂肽含量测定方法的改进

目的：优化脑蛋白水解物片中肽含量测定方法。方法：采用分子排阻色谱法鉴别脑蛋白水解物片中小分子肽类、高效液相色谱法测定脑蛋白水解物片中肽含量。结果：分子排阻色谱法鉴别小分子肽特异性强;高效液相色谱法测定的各种氨基酸在一定的浓度范围内与峰面积呈良好的线性关系（r＞0.999）,平均回收率为98.0%～102.4%。结论：该方法快速、简便、专属性强、重复性好,结果准确可靠,适用于脑蛋白水解物片的质量控制。     

Cow's milk IgE and IgG antibody responses to cow's milk formulas

For comparison of the antigenicity and allergenicity of three cow's milk formulas, serum IgE antibodies to cow's milk, β‐lactoglobulin and casein, and IgG antibodies to β‐lactoglobulin were analyzed in 94 infants with a family history of allergy. They were participating in a randomized trial comparing the allergy prophylactic effect of feeding an extensively hydrolyzed (N), a partially hydrolyzed (PH), and a regular cow's milk formula (RM). Only infants who had been formula‐fed for 3 months or more were included. IgE antibodies to cow's milk proteins were more common in the RM group (22/34) than in the N (2/31) and PH groups (3/29). There was a strong correlation between sensitization to cow's milk and β‐lactoglobulin ( r =0.85, P <0.001). The IgG responses to β‐lactoglobulin were low in the N group, intermediate in the PH group, and high in the RM group. High responses, as well as detection of IgE antibodies, were associated with development of atopic disease. The low antigenicity and allergenicity of the extensively hydrolyzed formula support its use in allergy prophylaxis. The partial hydrolysate seemed to be less suitable for this purpose.     

HPLC同时测定猪胆汁水解产物中猪去氧胆酸、鹅去氧胆酸的含量

目的:建立同时测定猪胆汁水解产物中猪去氧胆酸和鹅去氧胆酸含量的高效液相色谱方法。方法:采用C18色谱柱(4.6 mm×200 mm,5μm),以乙腈-0.1%磷酸(45∶55)为流动相,检测波长192 nm,流速1.0 mL·min-1,柱温35℃。结果:猪去氧胆酸在1.027~8.216μg具有良好的线性关系(r=0.999 9),平均回收率99.0%(RSD 1.94%)。鹅去氧胆酸进样量0.963~7.704μg具有良好的线性关系(r=0.999 0),平均回收率99.5%(RSD 1.76%)。结论:该方法灵敏、准确简便,适用于猪胆汁水解产物中猪去氧胆酸和鹅去氧胆酸的含量测定。     

Active Double-Layered Films Enriched with AgNPs in Great Water Dock Root and Pu-Erh Extracts

A novel, eco-friendly, and biocompatible method was applied to form silver nanoparticles (AgNPs) in great water dock (Lapathi radix) (KB) and pu-erh (Camellia sinensis) (PE) extracts. The surface plasma resonance peak of green synthesized AgNPs at 451.8 nm for AgNPs+KB and 440.8 nm for AgNPs+PE was observed via spectral analysis of UV absorbance. In this study, double-layered biopolymer films (FUR/CHIT+HGEL) with AgNPs incorporated in KB solution (AgNPs+KB) and AgNPs in PE solution (AgNPs+PE), were successfully prepared using the casting method. The SEM, XRD, zeta potential and size analyses confirmed the presence of AgNP in the films. The addition of AgNPs in plant extracts improved antimicrobial and antioxidant activity and thermal stability, whereas WVTR experienced a decrease. The nanocomposite films’ orange-brown colour may aid in the protection of food products against UV rays. The composite films demonstrated antibacterial activity against food-borne pathogens and may offer potential in food packaging applications.     

深度水解蛋白奶粉对过敏性紫癜儿童营养状况的应用研究

目的 探讨深度水解蛋白奶粉对过敏性紫癜儿童营养状况的作用和效果,为患儿寻找营养均衡的饮食治疗方法。方法 选取2014年1月-2015年6月在西安交通大学第二附属医院儿科住院治疗的过敏性紫癜患儿60例,随机将患儿分为限制饮食组30例,进食传统限制饮食;深度水解组30例,进食深度水解蛋白奶粉。同期选取在本科治疗的上呼吸道感染患儿30例作为正常对照组,进食正常饮食。于饮食治疗满1、2、3个月时分别给三组患儿进行膳食调查分析及血液生化检验。分析对比三组患儿营养素摄入水平和机体营养状况。结果 饮食治疗3个月后,限制饮食组大部分营养素摄入的实际/推荐百分比仍然低于60%,深度水解组全部营养素摄入的实际/推荐百分比达90%~110%之间,明显高于限制饮食组(P<0.05),与正常对照组相比差异无统计学意义(P>0.05)。深度水解组患儿的血清甘油三脂、胆固醇、前白蛋白、总蛋白和白蛋白水平与限制饮食组相比均明显升高(P<0.05),与正常对照组相比差异无统计学意义(P>0.05)。结论 深度水解蛋白奶粉应用于儿童过敏性紫癜饮食治疗中,提高患儿营养素及蛋白质的摄入水平,改善机体营养状况,利于患儿的健康成长。     

阿胶酶解液相对分子质量分布及其补血升白作用

目的:研究阿胶酶解液的相对分子质量分布及其补血、升白的作用。方法:采用TSK-GEL G2000SWXL色谱柱(7.8 mm×300 mm,5μm),流动相0.1 mol·L~(-1)NaCl+0.05%NaN_3+0.1 mol·L~(-1)磷酸盐缓冲液(pH 6.7),流速0.5 m L·min~(-1),检测波长280 nm,对阿胶原液、阿胶仿生酶解液、阿胶胰蛋白酶酶解液进行相对分子质量分布的测定;采用放血法致小鼠血虚模型及环磷酰胺致小鼠白细胞减少模型,观察阿胶酶解液的补血升白作用。结果:阿胶所含蛋白及多肽的相对分子质量6.64×10~4Da,胰蛋白酶酶解液及仿生酶解液的相对分子质量主要集中在3.7×10~3Da左右;阿胶仿生酶解液及胰蛋白酶酶解液可使血虚模型小鼠血红蛋白(Hb),红细胞计数(RBC)水平显著提高,使白细胞减少模型小鼠的白细胞计数(WBC),RBC和Hb水平显著提高。结论:阿胶经酶解后相对分子质量减小且具有显著的补血升白作用,胰蛋白酶酶解与仿生酶解效力大致相当。     

Production of antioxidant and ACE-inhibitory peptides from Kluyveromyces marxianus protein hydrolysates: Purification and molecular docking

Kluyveromyces marxianus protein hydrolysates were prepared by two different sonicated-enzymatic (trypsin and chymotrypsin) hydrolysis treatments to obtain antioxidant and ACE-inhibitory peptides. Trypsin and chymotrypsin hydrolysates obtained by 5 h, exhibited the highest antioxidant and ACE-inhibitory activities. After fractionation using ultrafiltration and reverse phase high performance liquid chromatography (RP-HPLC) techniques, two new peptides were identified. One fragment (LL-9, MW = 1180 Da) with the amino acid sequence of Leu-Pro-Glu-Ser-Val-His-Leu-Asp-Lys showed significant ACE inhibitory activity (IC₅₀ = 22.88 μM) while another peptide fragment (VL-9, MW = 1118 Da) with the amino acid sequence of Val-Leu-Ser-Thr-Ser-Phe-Pro-Pro-Lys showed the highest antioxidant and ACE inhibitory properties (IC₅₀ = 15.20 μM, 5568 μM TE/mg protein). The molecular docking studies revealed that the ACE inhibitory activities of VL-9 is due to interaction with the S2 (His513, His353, Glu281) and S′1 (Glu162) pockets of ACE and LL-9 can fit perfectly into the S1 (Thr345) and S2 (Tyr520, Lys511, Gln281) pockets of ACE.     

黄芪注射液治疗脑梗塞后抑郁症32例疗效观察

目的：探讨黄芪注射液对比脑蛋白水解物治疗脑梗塞后抑郁症的疗效观察.方法：回顾性分析2012年5月-2013年8月期间因脑梗塞后抑郁症入院的患者64例,按计划分为两组,每组32例,分别予以黄芪注射液（观察组）和脑蛋白水解物（对照组）,比较两组患者治疗后临床效果、HAMD评分、SDS评分以及不良反应发生情况.结果：观察组显效率78.1%明显高于对照组43.8%,治疗后HAMD评分（16.1±4.3）明显低于对照组（25.2±4.0）,SDS评分（51.7±3.6）明显低于对照组（59.9±4.1）,总不良反应15.7%明显低于对照组31.3%.结论：黄芪注射液对于治疗脑梗塞后抑郁症临床疗效明显,HAMD评分和SDS评分下降显著,不良反应发生率低,适合临床广泛应用.