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111.
112.
目的:建立测定地夫可特含量及有关物质的高效液相色谱方法。方法:采用VP-ODS色谱柱(250 mm×4.6 mm,5μm),以水-乙腈(60∶40)为流动相,流速1.0 mL.min-1,检测波长244 nm。结果:在上述色谱条件下,各中间体及降解产物均可与地夫可特主峰良好分离。地夫可特与有关物质测定的线性范围分别为50.2~201.0"g.mL-1(r=0.9999)与2.0~15.1"g.mL-1(r=0.9993);方法的重复性与中间精密度RSD分别为0.43%和0.68%。结论:本文方法简便,可用于测定地夫可特的含量及有关物质。 相似文献
113.
目的探讨水解蛋白在胃肠外科术后的临床应用价值及其护理措施。方法将32例胃肠手术患者随机分为试验组和对照组。试验组以水解蛋白为主要营养液进行肠内营养支持,以鼻肠管导入胃肠内;对照组以传统标准肠外营养液为主要营养支持,以中心静脉导管滴注提供营养。2组均提供等量的营养物质,比较2组术后8天、14天患者体质量、通气和通便时间、负氮平衡状态、尿中尿素氮排出量、血红蛋白和血浆白蛋白水平及免疫细胞指标CD4+、CD8+、CD4+/CD8+等生理指标。结果试验组通气和通便时间(2.54±0.32)天短于对照组(5.26±0.33)天。试验组在体质量降低、负氮平衡状态、尿中尿素氮排出量、血红蛋白和血浆白蛋白水平升高以及免疫细胞CD4+、CD8+及CD4+/CD8+升高方面均好于对照组。结论水解蛋白作为胃肠外科术后的肠内营养支持物质,能有效改善患者胃肠功能,提高患者机体免疫能力,促进患者恢复,具有良好的临床应用价值。规范的护理措施有利于提高胃肠术后患者对早期水解蛋白肠内营养的耐受性。 相似文献
114.
HPLC法测定茵栀黄注射液中水解产物没食子酸 总被引:1,自引:0,他引:1
目的建立灵敏度高、专属性强的茵栀黄注射液中鞣质的检测方法。方法以茵栀黄注射液为模型药物,采用高效液相色谱法测定注射液中水解没食子酸。Diamonsil ODS色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.05%磷酸水溶液(5︰95);体积流量1.0 mL/min;检测波长271 nm;柱温室温;进样量20μL。结果没食子酸进样量在36.125~231.20 ng与峰面积线性关系良好,平均回收率为99.6%,RSD为1.77%。茵栀黄注射液中不能检测出游离没食子酸,而水解没食子酸量较高,提示茵栀黄注射液中含有一定量的可水解鞣质。结论通过测定注射液中水解没食子酸,可以达到间接控制注射液中鞣质残留量的目的。 相似文献
115.
In a previous study, it was reported that yeast hydrolysate (YH) was effective in promoting bone growth in Sprague‐Dawley (SD) rats. To further clarify the mechanism of YH, the effects of YH on proliferation, differentiation and gene expression in vitro were investigated using osteoblastic cell lines (MC3T3‐E1). Cell proliferation increased significantly as much as 110% of the basal value when cells were treated with 100 µg/mL of YH. Alkaline phosphatase (ALP) activity increased significantly with a YH concentration of 25–100 µg/mL, and the activity increased 152% that of the control at 100 µg/mL. The calcium content increased as much as 129% at 100 µg/mL YH. The gene expression levels of ALP and collagen type II (COL II) significantly increased approximately 1.3‐fold and 1.7‐fold of control, respectively, at 100 µg/mL. YH increased significantly the mRNA level of bone sialoprotein (BSP) but not in a dose‐dependent manner. The mRNA levels of bone morphogenetic proteins (BMP)‐2, BMP‐4, collagen type I (COL I) and osteonectin (ON) did not increase. In summary, YH increased the proliferation of osteoblasts and directly stimulated ALP and bone matrix proteins (e.g. BSP, COL II), and these increases trigger osteoblastic differentiation (e.g. mineralized nodule formation). Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
116.
Hsiu‐Mei Chiang Jen‐Wen Lin Pei‐Ling Hsiao Shang‐Yuan Tsai Kuo‐Ching Wen 《Phytotherapy research : PTR》2011,25(4):569-576
The sun‐tanning process occurs as a spontaneous response to ultraviolet (UV) irradiation. UV will induce tanning and DNA damage, processes that can lead to photoaging and skin disorders such as hyperpigmentation and cancer. The pigment melanin protects skin from UV damage; therefore, an efficient melanin‐promoting suntan lotion could be highly beneficial. In this study, a process was developed to increase the content of naringenin in citrus extracts and to determine whether a higher naringenin content of citrus would induce melanogenesis. Melanin content and tyrosinase expression in mouse B16 melanoma cells were assayed after treatment with citrus plant extracts and their hydrolysates. The results indicate that hydrolysis increased the naringenin content in citrus extracts and that citrus preparations stimulated cellular melanogenesis and tyrosinase expression. It is suggested that this method is applicable to the industrial production of melanin‐promoting suntan lotions with antiphotocarcinogenic properties derived from citrus rind and citrus products. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
117.
118.
N.?A.?MazurkovaEmail author G.?P.?Troshkova T.?P.?Sumkina T.?D.?Kolokol’tsova M.?O.?Skarnovich A.?S.?Kabanov L.?N.?Shishkina A.?N.?Sergeev 《Bulletin of experimental biology and medicine》2008,146(4):547-550
We studied vaccine strains of influenza viruses A and B during their culturing in MDCK and Vero cells grown in Eagle’s MEM
medium and in a medium on the basis of enzyme hydrolysate of rise flour proteins with reduced (2%) content of fetal calf serum.
Optimal conditions for cell culturing and reproduction of influenza virus strains in these cells were studied. Culturing of
vaccine strains of influenza viruses in MDCK and Vero cells grown in nutrient media on the basis of rise flour protein hydrolysate
yielded high infection titers, which suggests that this medium can be used for the development of cultural influenza vaccine.
Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 4, pp. 234–237, November, 2008 相似文献
119.
Yvan Vandenplas Simona Belohlavkova Axel Enninger Pavel Frühauf Niten Makwana Anette Jrvi 《Nutrients》2021,13(9)
The purpose of this study was to evaluate the diagnosis and management of infants presenting with symptoms attributable to cow’s milk allergy (CMA) in a real life setting and to test how the Cow’s Milk-related Symptom Score (CoMiSS®) can be used to support the awareness to diagnose cow’s milk protein allergy in primary care practice. The CoMiSS is an awareness tool based on various symptoms such as crying, gastrointestinal symptoms, dermatological and respiratory symptoms. The study was conducted on 268 infants from four countries (Belgium, Czech Republic, Germany, UK) aged 0 to 18 months consulting for CMA related symptoms. The analysis was based on two visits of these subjects. The results show an average CoMiSS of 11 at the first visit. After a therapeutic dietary intervention, the score at the second visit, which happened 3 weeks ± 5 days after the first one, dropped to an average value of 4. A satisfaction questionnaire completed by the primary care practitioners suggested an overall high level of satisfaction with the application of the CoMiSS tool in routine practice. These data highlight a huge discrepancy in the diagnosis and management of infants suspected of CMA in the different countries. The findings suggest that the CoMISS questionnaire is an effective tool in aiding awareness of CMPA in primary health care. 相似文献
120.
目的 研究脑蛋白水解物(CH)-Ⅰ延缓D-半乳糖(D-gal)致C57小鼠衰老的作用机制。方法 C57/BL6N小鼠随机分为对照组、模型组、CH-Ⅰ低剂量组和CH-Ⅰ高剂量组,皮下注射D-gal以诱导衰老模型,给药组腹腔注射CH-Ⅰ。Morris水迷宫实验检测小鼠的学习和记忆能力,苏木素-伊红(HE)染色观察神经元的形态结构,Western印迹检测脑源性神经营养因子(BDNF)、信号传感器和转录激活因子(STAT)3/磷酸化(p)-STAT3和端粒酶逆转录酶(TERT)的表达,PCR-酶联免疫吸附试验(ELISA)检测端粒酶活性的表达。结果 与对照组相比,模型组学习记忆能力显著下降(P<0.01),海马神经元损伤明显增加(P<0.01),端粒酶活性明显降低(P<0.01),BDNF、TERT和p-STAT3表达水平下降(P<0.01)。而给予不同浓度的CH-Ⅰ预处理后,衰老小鼠的学习记忆能力障碍得到改善(P<0.05),海马神经元损伤显著下降(P<0.05),并且CH-Ⅰ低、高浓度均可以显著提高小鼠海马组织的端粒酶活性(P<0.05),上调BD... 相似文献