HPLC法测定小鼠脑内卡马西平的浓度

目的 利用高效液相色谱法建立脑内卡马西平浓度的测定方法.方法 脑组织加生理盐水,研磨至匀浆液,离心后取上清液加入乙腈沉淀蛋白.色谱柱为Inertsil ODS-SP柱（4.6×250mm,5μm）,流动相：水∶乙腈（60：40,V/V）,流速1.0mL·min-1,柱温30℃,检测波长235nm.结果 卡马西平线性范围为0.20～20.00μg·g-1（r=0.999）,相对回收率在98.83～102.12%,日内和日间精密度均小于6.9%.结论 该方法便捷、灵敏、准确,可用于卡马西平耐药机理、疗效提高等方面的研究.     

Understanding the Unconscious Mind: Jungian Psychology and Mental Health Nursing

How might the unconscious part of the mind affect mental health patients’ emotions or behaviour? How might the unconscious motivations of mental health nurses affect their patients? The discovery of “the unconscious” two centuries ago has allowed philosophers and scientists, such as C. G. Jung, to explore the field. Contemporary mental health care subscribes to a dominance of neurobiological approaches, neglecting the unconscious or relegating it to that of a merely biological process. Approaching this subject from the perspective of Jung, we make a case for the inclusion of theoretical concepts about the unconscious in the discourse of mental health nursing. Such awareness may help mental health nurses to better understand the mental disease, disorder, and distress found in patients. It also may help them understand their own conflicts and motivations that, in turn, can have an affect on their patients.     

卡马西平配合手法按摩治疗癫痫大发作的疗效评价

目的研究卡马西平配合手法按摩对癫痫大发作的疗效。方法采用前瞻性的研究方法，将癫痫大发作患者随机分为实验组和对照组各40例。实验组在口服卡马西平基础上给予手法按摩；对照组给予口服卡马西平治疗，比较2组的治疗效果。结果实验组治疗的总有效率为92．5％，显著高于对照组的80．0％。结论卡马西平配合手法按摩治疗癫痫大发作能够明显地提高疗效，值得推广应用。     

In Vitro Characterization of Carbamazepine-Loaded Precifac Lipospheres

Lipospheres of carbamazepine were prepared by melt dispersion technique using Precifac® ATO 5 in the various drug-lipid ratios. The resulting free-flowing lipospheres were evaluated with respect to surface morphology, particle size distribution, encapsulation efficiency, and in vitro release behavior. The effect of druglipid ratio, the surfactant added, emulsion stabilizer, and stirring speed also were identified as the key variables affecting the formation of discrete spherical lipospheres and drug release rate. The preparation conditions were optimized by using 0.4% w/v span 20 (Hydrophilic-Lipophilic Balance, HLB=8.6) as a surfactant and 1% w/v gelatin solution as a stabilizer in presence of a high level of water.We found that the ratio of drug to lipid affects the size of the spheres. The incorporation efficiency was found to be high at all loadings. Increasing the lipid:drug ratio produced more spherical, smooth, and round lipospheres. All the prepared lipospheres exhibited slow release profiles dictating the Higuchi mode of release. We saw that the higher the sphere size and the ratio of Precifac®, the slower is in vitro drug release.     

A pediatric approach to management of skin growths in basal cell nevus syndrome

Little guidance on management of basal cell nevus syndrome in children exists. We report a case series of four patients diagnosed with BCNS in early childhood, in whom several highly suspicious lesions were biopsied, but several smaller and questionably concerning lesions were treated with therapies that are more tolerable for children, including topical imiquimod, 5-fluorouracil, cryotherapy, or touch electrodessication following topical anesthetic cream. These therapies were well tolerated, and all residual or persistent lesions were subsequently biopsied and found to be benign. This approach is often preferable for pediatric BCNS patients, in whom concerning lesions can be identified clinically and managed compassionately. However, any lesion that exhibits growth, bleeding, or symptoms should be biopsied for definitive diagnosis.     

Harnessing PGPR inoculation through exogenous foliar application of salicylic acid and microbial extracts for improving rice growth

Plant-growth-promoting rhizobacteria (PGPR) should effectively colonize along the plant root to enhance the plant and soil health. The present investigation aims to improve the PGPR-mediated plant health benefits through above-ground foliar management. A green fluorescent protein-tagged PGPR strain, Pseudomonas chlororaphis (ZSB15-M2) was inoculated in a nonautoclaved agricultural soil before rice culturing. Salicylic acid and cell extracts of Corynebacterium glutamicum and Saccharomyces cerevisiae as a supply of hormonal and inducer compounds were applied on the foliage of the 10-days-old rice plants and subsequently observed the colonizing ability of ZSB15-M2. The cell extracts of Corynebacteria and yeast showed a 100-fold increase in the ZSB15-M2 population in the rhizosphere of rice, whereas salicylic acid had a 10-fold increase in relation to mock control. The rice root exudates collected after the spraying of salicylic acid and microbial extracts showed significantly enhanced release of total carbon, total protein, total sugar, total amino nitrogen, total nitrogen, and phenol content. In vitro assays revealed that these root exudates collected after exogenous spray of these chemicals enhanced the chemotactic motility and biofilm formation of ZSB15-M2 compared to the control plant's root exudate. Metabolomic analysis of root exudates collected from these rice plants by gas chromatography–mass spectrometry revealed that the Corynebacteria and yeast cell extracts enhanced the divergence of metabolites of rice root exudate. Further, due to these cumulative effects in the rice rhizosphere, the total chlorophyll, total protein, total nitrogen, and total phosphorus of rice were significantly improved. These observations provide insights into the rhizosphere functioning of rice plants as modulated by above-ground treatments with improved colonization of inoculant strains as well as the plant growth.     

大学生亲子亲和与自我同一性:孤独感的中介作用

目的:考察大学生亲子亲和对自我同一性的影响,并探讨孤独感在二者之间的中介作用。方法:采用亲子亲和问卷、孤独感量表(UCLA)、自我同一性状态问卷对333名大学生进行问卷施测。结果:(1)亲子亲和与自我同一性获得状态显著正相关(r_(母亲)=0.11,r父亲=0.12;P0.05),扩散状态显著负相关(r_(母亲)=-0.18,P0.01;r父亲=-0.11,P0.05);(2)亲子亲和与孤独感显著负相关(r_(母亲)=-0.33,r_(父亲)=-0.30;P0.01);(3)孤独感与自我同一性获得状态显著负相关(r=-0.22,P0.01),与延缓(r=0.15,P0.01)、早闭(r=0.20,P0.01)、扩散(r=0.18,P0.01)显著正相关;(4)孤独感在亲子亲和与同一性获得状态、扩散状态的关系中起完全中介作用。结论:亲子亲和是大学生自我同一性发展的有效保护因子,且孤独感是两者关系的重要中介机制。     

针刺治疗原发性三叉神经痛的疗效观察

目的：观察原发性三叉神经痛针灸治疗的临床疗效。方法：136例均为原发性三叉神经痛患者，分为对照组、针刺治疗组。结果：祖国医学针灸治疗原发性三叉神经痛效果明显。结论：针灸治疗原发性三叉神经痛简便易行，值得推广。