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991.
992.
目的研究代谢性谷氨酸受体第5亚型(mGluR5)在牙髓各部位中的表达及分布。方法收集2009年7月至2010年1月山东大学口腔医院口腔颌面外科因正畸或其他治疗需要而拔除的健康前磨牙或第三磨牙5例,制成一系列石蜡切片,利用免疫组化方法检测牙髓各部位mGluR5的表达及分布情况,利用图像分析系统对其表达强度进行半定量分析,探讨mGluR5在牙髓中的作用和意义。结果正常牙髓从冠部、颈部到根部牙髓成牙本质细胞中mGluR5表达均呈阳性,且由冠部、颈部到根部mGluR5表达强度依次降低。结论mGluR5在牙髓疼痛传递过程中可能具有一定的作用。 相似文献
993.
Irritable bowel syndrome (IBS) global burden is underestimated despite its high prevalence. It’s a gastrointestinal disease having obscure pathophysiology with multiple therapies yet unsatisfactory remedies. The Endocannabinoid system (ECS) of our body plays a key role in maintaining normal physiology of the gastrointestinal tract as well as involves abnormalities including functional diseases like IBS. This review highlights the importance of the Endocannabinoid system, its connections with the normal gastrointestinal functions and abnormalities like IBS. It also discusses the role of cannabis as medical therapy in IBS patients. A literature search for articles related to endocannabinoids in IBS and medical cannabis in PubMed and Google Scholar was conducted. The studies highlighted the significant participation of ECS in IBS. However, the breach in obtaining the promising therapeutic model for IBS needed further investigation in ECS and uncover other treatments for IBS. This review summarizes ECS, highlights the relationship of ECS with IBS and explores cannabis as a potential therapy to treat IBS. 相似文献
994.
Ruyi Cong Xiaoyu Zhang Zihong Song Shanshan Chen Guanhua Liu Yizhi Liu Xiuyu Pang Fang Dong Weijia Xing Youxin Wang Xizhu Xu 《Nutrients》2022,14(5)
Previous observational studies have highlighted associations between adipokines and hyperuricemia, as well as gout, but the causality and direction of these associations are not clear. Therefore, we attempted to assess whether there are causal effects of specific adipokines (such as adiponectin (ADP) and soluble leptin receptors (sOB-R)) on uric acid (UA) or gout in a two-sample Mendelian randomization (MR) analysis, based on summary statistics from large genome-wide association studies. The inverse-variance weighted (IVW) method was performed as the primary analysis. Sensitivity analyses (including MR-Egger regression, weighted median, penalized weighted median, and MR pleiotropy residual sum and outlier methods) were also performed, to ensure reliable results. In the IVW models, no causal effect was found for sOB-R (odds ratios (OR), 1.002; 95% confidence intervals (CI), 0.999–1.004; p = 0.274) on UA, or ADP (OR, 1.198; 95% CI, 0.865–1.659; p = 0.277) or sOB-R (OR, 0.988; 95% CI, 0.940–1.037; p = 0.616) on gout. The results were confirmed in sensitivity analyses. There was no notable directional pleiotropy or heterogeneity. This study suggests that these specific adipokines may not play causal roles in UA or gout development. 相似文献
995.
目的:探讨D期前列腺癌(PCa)患者药物去势治疗的效果与雄激素受体(AR)、前列腺特异性抗原(PSA)的相关性。方法:对33例D期PCa患者给予LHRH类似物 抗雄激素药物进行药物去势治疗,结合免疫组化进行统计分析。结果:AR、PSA与癌组织分化程度的之间的差异有统计学意义。Gleason不同评分组间血清PSA水平之间差异有统计学意义(P<0.05),PCa Gleason评分值与原位PSA免疫标记表达呈明显的负相关性(P<0.01);原位PSA免疫标记表达与血清PSA不具有相关性。结论:PCa组织中AR、PSA表达与D期PCa患者行药物去势疗法疗效有密切关系;血清PSA变化是监测PCa肿瘤复发与疗效的可靠瘤标。 相似文献
996.
目的观察外周炎症时大鼠背根神经节(dorsalrootganglion,DRG)细胞μ-和κ-阿片受体mRNA表达的变化。方法用角叉菜胶(carrageenan,CAR)在大鼠右后足皮下注射产生外周炎症,分别于正常和炎症后6、12、24和72h取右L4~5DRG。用原位杂交的方法观察正常及炎症期间DRG细胞μ-和κ-阿片受体mRNA表达的变化。结果正常大鼠的部分DRG细胞内可见μ-和κ-阿片受体mRNA表达。外周炎症后6h,表达μ-和κ-阿片受体mRNA的DRG细胞的数和μ-和κ-阿片受体mRNA表达量增加,24h表达量最高,72h下降至正常。结论外周炎症能使大鼠DRG细胞μ-和κ-阿片受体mRNA表达增加。提示外周炎症时大鼠DRG细胞μ-和κ-阿片受体合成增加。为阿片类物质应用于外周镇痛提供理论依据。 相似文献
997.
Ryan M. Carey Derek B. McMahon Zoey A. Miller TaeBeom Kim Karthik Rajasekaran Indiwari Gopallawa Jason G. Newman Devraj Basu Kevin T. Nead Elizabeth A. White Robert J. Lee 《Molecular oncology》2022,16(7):1474
Better management of head and neck squamous cell carcinomas (HNSCCs) requires a clearer understanding of tumor biology and disease risk. Bitter taste receptors (T2Rs) have been studied in several cancers, including thyroid, salivary, and GI, but their role in HNSCC has not been explored. We found that HNSCC patient samples and cell lines expressed functional T2Rs on both the cell and nuclear membranes. Bitter compounds, including bacterial metabolites, activated T2R‐mediated nuclear Ca2+ responses leading to mitochondrial depolarization, caspase activation, and ultimately apoptosis. Buffering nuclear Ca2+ elevation blocked caspase activation. Furthermore, increased expression of T2Rs in HNSCCs from The Cancer Genome Atlas is associated with improved overall survival. This work suggests that T2Rs are potential biomarkers to predict outcomes and guide treatment selection, may be leveraged as therapeutic targets to stimulate tumor apoptosis, and may mediate tumor‐microbiome crosstalk in HNSCC. 相似文献
998.
Juan Jin Tao-jie Zhou Gui-ling Ren Liang Cai Xiao-ming Meng 《Acta pharmacologica Sinica》2022,43(11):2789
Nucleotide-binding oligomerization domain-like receptors (NLRs), including NLRAs, NLRBs (also known as NAIPs), NLRCs, and NLRPs, are a major subfamily of pattern recognition receptors (PRRs). Owing to a recent surge in research, NLRs have gained considerable attention due to their involvement in mediating the innate immune response and perpetuating inflammatory pathways, which is a central phenomenon in the pathogenesis of multiple diseases, including renal diseases. NLRs are expressed in different renal tissues during pathological conditions, which suggest that these receptors play roles in acute kidney injury, obstructive nephropathy, diabetic nephropathy, IgA nephropathy, lupus nephritis, crystal nephropathy, uric acid nephropathy, and renal cell carcinoma, among others. This review summarises recent progress on the functions of NLRs and their mechanisms in the pathophysiological processes of different types of renal diseases to help us better understand the role of NLRs in the kidney and provide a theoretical basis for NLR-targeted therapy for renal diseases. 相似文献
999.
目的 评价湖北金粟兰中倍半萜二聚体化合物(+)-chlorahupetenes B[(+)-CHB]对脂多糖(LPS)诱导的小鼠巨噬细胞(RAW264.7)炎症反应的影响及作用机制。方法 RAW264.7细胞分为对照组(给予等体积DMSO)、模型组(给予等体积DMSO)、地塞米松磷酸钠注射液(Dex,阳性对照,1 μmol·L-1)组和(+)-CHB低、中、高浓度(5、10、20 μmol·L-1)组。各组分别加入相应药物孵育细胞1 h,除对照组外,其余组加入LPS (1 μg·mL-1)诱导24 h造成炎症应答模型。细胞增殖检测法用于评估细胞活力;Griess反应检测一氧化氮(NO)浓度;酶联免疫分析检测炎症细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β、IL-6的生成;实时荧光定量PCR (qRT-PCR)检测IL-1β、NOD样受体热蛋白结构域相关蛋白3(NLRP3)、环氧合酶-2(COX-2)、诱导型一氧化氮合酶(iNOS)、IL-6和TNF-α mRNA表达;Western blotting检测Toll样受体4(TLR4)、髓样分化因子88(MyD88)、核因子κB (NF-κB) p65、p-NF-κB p65、NLRP3、嘌呤能受体(P2X7)蛋白表达水平。结果 与模型组比较,(+)-CHB减少了梭形细胞数量,使大部分细胞恢复正常形态;显著抑制NO、TNF-α、IL-6、IL-1β生成(P<0.01),显著降低COX-2、iNOS、IL-6、TNF-α、NLRP3、IL-1β mRNA水平(P<0.05、0.01);显著降低TLR4、MyD88、NF-κB p65、p-NF-κBp65、NLRP3、P2X7蛋白表达水平(P<0.05、0.01)。结论 (+)-CHB通过抑制TLR4/MyD88/NF-κB信号通路和P2X7/NLRP3/IL-1β炎症小体轴激活缓解LPS诱导的巨噬细胞炎症反应。 相似文献
1000.
为了研究反义核苷酸对平滑肌细胞(SMC)及生长因子基因表达的影响。本实验对兔髂动脉粥样硬化模型行血管成形术;应用Northern印迹杂交和RT-PCR方法观察反义核苷酸对体外兔髂动脉SMC增殖及转化生长因子-β1(TGF-β1)、表皮生长因子受体(EGFR)、碱性成纤维细胞生长因子(bFGF)基因表达的影响。结果表明:反义核苷酸抑制SMC增生,并呈浓度依赖性;反义核苷酸抑制TGF-β1和bFGFmRNA表达;阳离子脂质体能明显增强反义核苷酸的上述作用。在培养的兔髂动脉SMC中EGFRmRNA表达阴性。结论:反义核苷酸抑制兔髂动脉SMC增生与抑制TGF-β1和bFGFmRNA表达有密切关系。 相似文献