增生性瘢痕中血管内皮细胞生物学功能的研究

目的 探讨增生性瘢痕中血管内皮细胞的生物学功能及其与瘢痕形成的关系。方法 取人增生性瘢痕组织和正常皮肤组织，进行组织学观察。分离和纯化2种标本中的血管内皮细胞，应用酶联免疫吸附测定法分别检测单个血管内皮细胞中转化生长因子β1，（TGF-β1）、成纤维细胞生长因子2（FGF2）、血小板源性生长因子（PDGF）、内皮素1（ET-1）和血管内皮生长因子（VEGF）的水平。结果光学显微镜下可见正常皮肤微血管数目较少；增生性瘢痕微血管数目增多，血管狭长扭曲甚至闭塞。透射电镜可见增生性瘢痕中毛细血管管腔狭窄，有内皮细胞脱落。增生性瘢痕中血管内皮细胞分泌TGF-β1、PDGF、ET-1、VEGF、FGF2的水平分别为（60±8）、（30±4）、（0．12±0．03）、（52±5）、（18．1±1．2）μg／个细胞，明显低于正常皮肤（P〈0．05）。结论 增生性瘢痕中血管内皮细胞生物学功能减退，可能与瘢痕中胶原的大量产生和缺氧有关。     

囊性纤维化病相关基因突变及其检测的研究进展

囊性纤维化（cystic fibrosis，CF）是白种人中最常见的致死性常染色体隐性遗传病，其发病率在西、北欧及北美人群中较高，约占活产婴儿的1／2500，但在亚洲人的发病率较低，约为1／10万，中国台湾仅报道有6例CF患者。该病的致病基因又称为CF基因，在正常白种人中CF基因突变携带者频率为2％～4％，发病率为0．2％。     

浅析共情的概念及对护患关系的影响

人的基本需要之一是被别人理解。如果作为个体的独特性能被其他人正确地理解，就会有深切的满足感，相互之间的关系也会进一步深入下去。正足这种理解构成了建立关系的基础。护士和病人之间发展起来的关系即护患关系，是护理实践的基础。建立良好的护患关系，使病人感受到治疗性的支持，有利于病人的康复；可以促进护患之间的理解，减少医疗纠纷；同时还可避免护士的耗竭。理解病人     

Changes in T .lymphocyte subsets after severe traumatic brain inJury

BACKGROUND： Besides local changes of cranial parenchymal cells, hemorrhage, etc., severe traumatic brain injuries also cause the changes of total body fluid and various functions, and the changes of lymphocytes and T lymphocyte subsets should be paid more attention to. OBJECTIVE： To reveal the changing laws of T lymphocyte subsets after severe traumatic brain injury, and compare with mild to moderate brain injury. DESIGN： A comparative observation. SETTINGS： Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City; Central Laboratory of Shenzhen Hospital of Prevention and Cure for Chronic Disease. PARTICIPANTS： All the subjects were selected from the Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City from August 2002 to August 2005. Thirty patients with severe brain injury, whose Glasgow coma score （GCS） was ≤ 8 points, were taken as the experimental group, including 21 males and 9 females, aging 16 - 62 years. Meanwhile, 30 patients with mild traumatic brain injury were taken as the control group （GCS ranged 14- 15 points）, including 18 males and 12 females, aging 15 -58 years. All the subjects were in admission at 6 hours after injury, without disease of major organs before injury Informed consents were obtained from all the patients or their relatives. METHODS： （1） The T lymphocytes and the subsets in peripheral blood were detected with immunofluorescent tricolor flow cytometry at l, 3, 7 and 14 days after injury in both groups. （2） The conditions of pulmonary infections were observed at 4 days after injury. The differences of measurement data were compared with the t test. MAIN OUTCOME MEASURES： Changes of T lymphocytes subsets at 1 - 14 days after severe and mild or moderate traumatic injury. RESULTS： Finally, 28 and 25 patients with mild to moderate traumatic brain injury, whereas 25 and 21 patients with severe traumatic brain injury were analyzed at 7 and 14 days respectively, and the missed ones died due to the development of disease. （1） Changes of T lymphocyte subsets： At 1 and 3 days after injury, CD3, CD4, CD8, CD4/CD8 began to decrease, whereas CD8 increased in the experimental group, which were very significantly different from those in the control group （t =2.77 - 3.26, P 〈 0.01）, and began to recover at 7 days, which were significantly different from those in the control group （t = 2.06 - 2.24, P 〈 0.05）, and generally recovered to the normal levels at 14 days （P 〉 0.05）. （2） Conditions of pulmonary infections： At 4 days after injury, the rate of pulmonary infection was significantly different between the experimental group and control group [73% （22/30）, 0, x2=37.29, P 〈 0.01]. CONCLUSION： Patients with severe traumatic brain injury suffer from damages of cellular immune function at early period （within 7 days）, and they are easily to be accompanied by pulmonary infections.     

SPECT脑血流灌注显像2D ROI和3D VOI对脑内血肿吸收的评估比较

本研究探讨高血压性脑出血患者SPECT脑血流灌注显像中二维感兴趣区（2DROI）和三维感兴趣区体积（3DVOI）对脑内血肿吸收的评估比较，现报道如下。     

学习障碍患儿血清BDNF水平的研究

目的探讨学习障碍血清脑源性神经营养因子(BDNF)水平的变化,及其与学习障碍病理基础的关系。方法患者组为22例未治疗过且不共患其他疾病的学习障碍患儿,对照组为16例年龄性别匹配正常儿童,以瑞文标准推理测验(SPM)测定智商,BDNF采用酶联夹心免疫吸附法检测。结果LD患者血清BDNF浓度为:平均(4.603±3.620)ng/ml高于对照组(1.843±0.728)ng/ml(t=3.326,P=0.003<0.01)。边缘智商组(4.523±4.618)ng/ml,与正常对照组比较差异有非常显著性(P=0.008<0.01)。结论学习障碍患儿血清BDNF浓度高于正常儿童,边缘智商者升高更明显。     

脑出血后的抑制细胞凋亡干预

脑出血后血肿周围组织可发生继发性损伤。研究表明，细胞凋亡在脑出血后继发性损伤中起着重要作用。抑制细胞凋亡可显著改善脑出血后的神经功能缺损。     

“大疱性皮肤病学术研讨会”会议通知

上海医学会皮肤性病学专业委员会、上海交通大学医学院附属瑞金医院于2007年3月13日在瑞金医院召开“大疱性皮肤病学术研讨会”。届时国际著名天疱疮研究专家，美国宾夕法尼亚大学皮肤科主任John Stanley和日本清音大学医学院皮肤科主任Masayuki Amagai教授将进行专题报告，同时也将邀请国内大疱病专家演讲，并希望自由发言。     

Mirrizi综合征48例治疗分析

目的：探讨Mirrizi综合征的早期有效治疗方法。方法：回顾性分析2001年5月∽2004年4月我院收治48例Minizi综合征患者，其中28例采用早期手术治疗，其余经保守治疗好转后改手术治疗，或保守治疗无效改手术治疗，并辅以抗炎、支持等治疗方法。结果：经早期手术治疗的患者疗效满意，预后较好。非早期手术者，有8例出现不同程度并发症，1例死亡。结论：Mirrizi综合征的早期手术治疗是必要的、可行的。     

某部战士性心理及性行为的调查与分析

目的了解战士性心理和相关状况。方法用自编的调查表对某部204名战士进行了调查。结果对性问题表示关心的占22．7％(女)=50．6％(男)，愿意与异性交往的占59．1％(女)-75．0％(男)，谈过恋爱的占51．3％(男)-54．6％(女)，有过性幻想的占56．8％(女)-82．5％(男)，有过性梦的占38．7％(女)-95．7％(男)，手淫发生率20．5％(女)-55．6％(男)，性行为发生率9．1％(女)-40．6%(男)，封婚前性行为持认可态度者占40．9％(女)-65．0％(男)．结论当前部队战士性心理和性行为状况与地方青少年相比既有共同性，又有特殊性。为促进战士身心健康成长，对战士的性教育势在必行。