Juvenile spot were administered Kepone® (chlordecone) by ingestion. Body burdens of Kepone increased additively and equilibrium was not attained. When spot were fed a lethal concentration (3.3 μg/g) over 28 days, they accumulated an average of 2.7 μg/g (wet wt). Most of them developed muscular tetany, fractured vertebral centra, and abnormally thickened vertebrae. These symptoms developed over a 4-wk period until death. Spot fed lesser concentrations (0.59 and 0.30 μg/g) accumulated 0.7 and 0.3 μg/g over 56 days and developed similar bone damage. The percentage of collagen in vertebrae was significantly greater in the treated fish which had thickened bones. Spot that were held in the laboratory as controls for 5 wk had significantly lower collagen percentages than wild fish. These results confirm Kepone accumulation as the likely mechanism for production of the bone deformities observed in fishes from the James River in 1971–1976. 相似文献
The importance of reliable exposure assessment, as a key component of the overall risk assessment process, has been well described for some considerable time. Yet, despite this widely accepted tenet, many studies conclude significant adverse health effects, with associated public policy implications, in the absence of adequate or, in some cases, even rudimentary, exposure quantification. Moreover, it appears that epidemiological studies in humans and toxicological studies in experimental animals may both suffer from inadequate exposure assessment. In this review, we discuss the nature and quality of the exposure assessment in both epidemiologic and toxicologic studies using examples from the pesticides and phthalate literature. Each type of study has its strengths and weaknesses in how exposure is assessed and often the strength of one is also a weakness. It would appear that insufficient or incomplete information about differences in exposure assessment could explain, at least in some cases, the differences in outcome between toxicological and epidemiological studies. Research efforts should focus on improving the feasibility of including biomonitoring in both animal and human studies to facilitate comparisons between animal and human models and improve exposure assessment in epidemiologic studies. Animal and human studies should measure the same biomarkers, where possible, to facilitate human health risk assessment. 相似文献
AbstractThe effect of non-functionalized and amino-functionalized multiwall carbon nanotube (CNT) exposure, as well as the impact of CNT presence on coexistent pesticide accumulation, was investigated in lettuce (Lactuca sativa L.). Lettuce seeds were sown directly into CNT-amended vermiculite (1000?mg?L?1) to monitor phytotoxicity during germination and growth. During growth, lettuce seedlings were subsequently exposed to chlordane (cis-chlordane [CS], trans-chlordane [TC] and trans-nonachlor [TN]) and p,p′-DDE (all at 100?ng/L) in the irrigation solution for a 19-d growth period. CNT exposure did not significantly influence seed germination (82–96%) or plant growth. Similarly, pesticide exposure had no impact on plant growth, total pigment production or tissue lipid peroxidation. After 19?d, the root content of total chlordane and p,p′-DDE was 390 and 73.8?µg?g?1, respectively; in plants not exposed to CNTs, the shoot levels were 1.58 and 0.40?µg?g?1, respectively. The presence and type of CNT significantly influenced pesticide availability to lettuce seedlings. Non-functionalized CNT decreased the root and shoot pesticide content by 88% and 78%, respectively, but amino-functionalized CNT effects were significantly more modest, with decreases of 57% in the roots and 23% in the shoots, respectively. The presence of humic acid completely reversed the reduced accumulation of pesticides induced by amino-functionalized CNT, likely due to strong competition over adsorption sites on the nanomaterial (NM). These findings have implications for food safety and for the use of engineered NMs in agriculture, especially with leafy vegetables. 相似文献
Paraoxonases and cytochromes P450 constitute two major classes of xenobiotic-metabolizing enzymes involved in the detoxification of pesticide chemicals. In this study, we examined the distribution of two common genetic polymorphisms of the paraoxonase 1 gene and one common polymorphism of the CYP1A1 gene, in relation to pathological diseases occurring in a rural population.
Blood and hair samples were collected from 220 participants of an agricultural cohort in the south of Greece for genotype and pesticide analysis. Demographic information and disease status of the participants was obtained by questionnaire, medical examination and medical record. Organochlorine pesticides and metabolites (DDTs, HCHs) were extracted from hair and analyzed using gas chromatography combined with mass spectrometry techniques.
Our results indicate exposure of the rural population of Amaliada to organophosphate and past exposure to organochlorine pesticides.
Genotypic analysis of PON1Q192R, PON1L55M and CYP1A1*2A MspI polymorphisms was performed using PCR-RFLP. The PON1 192R and 55M alleles absence was significantly associated with hypertension (OR: 2.59; 95% CI: 1.10–6.09) and hepatitis (OR: 21.43; 95% CI: 2.53–181.50), respectively, as indicated from backward logistic regression. Although the presence of PON1 192R allele significantly affected the occurrence of prostate hyperplasia (OR: 0.35; 95% CI: 0.03–0.40), no associations were obtained between the paraoxonase serum activity or the CYP1A1 genotype and the disease status.