维生素B12和叶酸干预对绝经后骨质疏松症妇女的骨代谢及同型半胱氨酸水平的影响

目的观察叶酸和维生素B12对绝经后骨质疏松症妇女的骨代谢及同型半胱氨酸水平的影响。方法 80例绝经后骨质疏松症妇女参加研究。所有参与者随机接受叶酸和维生素B12(n=40)或安慰剂(n=40)治疗。在干预前的基线及干预后3个月和6个月,测量两组患者血清同型半胱氨酸、维生素B12和骨代谢标志物的水平。结果治疗前,两组患者血清同型半胱氨酸、维生素B12和骨代谢标志物水平比较差异无统计学意义(P0.05)。治疗后,两组患者同型半胱氨酸均下降,但比较差异无统计学意义(P0.05)。6个月后各组间血清维生素B12、骨钙素、CTX的变化均有显著改变且差异有统计学意义(P0.05)。结论绝经后骨质疏松症妇女补充叶酸和维生素B12可以一定程度改善同型半胱氨酸及骨代谢指标水平。     

毒理基因组学在生物标志物研究中的应用

近年来,毒理基因组学的快速发展为生物标志物的研究提供了一个更广阔的空间。生物标志物作为一种可以客观衡量和评价正常生理、病理过程及治疗药物药理学效应的指标,如今已被广泛应用于临床诊断和新药开发等多个领域。就毒理基因组学在发现和筛选生物标志物方面的研究现状和进展进行综述。     

急性心肌梗死后心脏生物标志物的变化及其与左心功能和左心室重构的关系

目的研究急性心肌梗死（AMI）后心脏生物标志物的变化及其与左心室功能和左心室重构的关系。方法选取45例AMI并于12h内成功施行直接经皮冠状动脉介入治疗（PPCI）的患者作为研究对象,分别于AMI后第2天、第1周、第1个月监测心脏生物标志物如高敏C-反应蛋白（hs-CRP）、肌钙蛋白（TnI）、N末端脑钠肽前体（NT-pro—BNP）等的血浆浓度;测量AMI后3个月时左心功能[选取左室射血分数（LVEF）为测量指标]和左心室重构[选取左室舒张末内径（LVED）作为测量指标],并比较它们之间的关系。结果hs-CRP、TnI在PPCI后第2天一第1个月持续显著下降,NT—pro—BNP在PPCI后第2天一第1周显著下降（P〈0．05）,第1周～第1个月无显著变化。再灌注2d后,hs—CRP血浆浓度与LVEF、LVED有相关性,且相关性较好;第2天与第1周的TnI与LVEF有相关性,且相关性较好;血清NT-pro—BNP在第2天与LVEF有相关性,在第1周、第1个月与LVEF、LVED有相关性,相关性较好。第2天的hs—CRP、TnI与3个月后的LVEF呈负相关,相关性较好;与3个月后的LVED相关,相关性较好。结论AMI后1个月内,hs-CRP、TnI持续显著降低,1周内NT—pro—BNP显著降低。第2天的hs-CRP和TnI能预测3个月的左心功能、左心宣亏构．     

Consumption of Vegetables Is Associated with Systemic Inflammation in Older Adults

It is hypothesized that healthy diets rich in fruits and vegetables (FV) can modulate the inflammatory status in older adults. However, to determine the actual impact of FV on inflammatory status, adiposity level and objectively assessed physical activity (PA) behaviors need to be considered. The aim of the present study was to explore associations between FV intake and biomarkers of systemic inflammation in older adults. Based on a sample of 233 older adults (65–70 years old), the following inflammatory biomarkers were assessed: C-reactive protein (CRP), fibrinogen, interleukin-6 (IL-6), IL-10, IL-18, and monocyte chemoattractant protein-1 (MCP-1). FV intake was assessed by self-report, and PA behaviors encompassing time spent sedentary and in moderate-to-vigorous PA (MVPA) were determined using accelerometers. Associations between FV intake and inflammatory biomarkers were analyzed using stepwise linear regression models while adjusting for several covariates, including health-related food groups, adherence to the MVPA guidelines, total sedentary time, and waist circumference. While no significant associations were observed for the total FV intake, the vegetable intake was inversely associated with levels of IL6 (β = −0.15; p < 0.05). In contrast, fruit intake was not associated with any inflammatory biomarker. In conclusion, our findings indicate beneficial associations between vegetable intake and levels of a pro-inflammatory biomarker in older adults, which strengthens public health efforts to promote vegetable-rich diets in older adults to mitigate age-related systemic inflammation.     

应用二维电泳和质谱技术鉴定乳腺癌相关蛋白

目的 通过比较人乳腺癌细胞MCF7和乳腺上皮细胞MCF10A的差异表达蛋白,以发现可能用于早期诊断的乳腺癌肿瘤标志物.方法 体外培养人乳腺癌细胞MCF7和乳腺上皮细胞MCF10A,提取细胞总蛋白,进行二维凝胶电泳并比较分析,选择在乳腺癌细胞MCF7中明显差异表达的蛋白点,进行质谱和生物信息学分析.结果 建立了MCF7和MCF10A两种细胞系的二维凝胶电泳图谱,平均蛋白质点数分别为(960±44)和(1128±29);对选择的35个差异表达蛋白质点进行质谱和生物信息学分析,鉴定了其中16个点,包括过氧化氢酶-6(PRDX6)、磷酸甘油酸激酶-1(PGK1)、60 kD热休克蛋白(CH60)、谷氨酰胺转移酶-2(TGM2)等.结论 乳腺癌细胞MCF7相对于乳腺上皮细胞MCF10A的差异表达蛋白可能作为乳腺癌早期诊断和预后评价的候选肿瘤标志物.     

血浆ESM-1对脓毒症ARDS发生预测价值的研究

目的 探讨血浆内皮细胞特异性分子-1（endothelial cell specific molecule-1, ESM-1）对脓毒症性急性呼吸窘迫综合征(acute respiratory distress syndrome, ARDS)发生的预测价值。方法 本研究共纳入符合脓毒症诊断标准患者54例,根据在72h内是否并发ARDS将患者分为非ARDS组（n=42例）与ARDS组（n=12例）。分别检测入组第1、2、3、7天患者血浆ESM-1、白细胞（white blood cell, WBC）、C反应蛋白（C-reactive protein, CRP）、白介素6（interleukin 6, IL-6）、降钙素原（procalcitonin, PCT）水平,并计算氧和指数（oxygenation index, OI）。结果 入组第1天的ARDS组患者血浆ESM-1浓度水平明显低于非ARDS组（t=2.778,P=0.009）。血浆ESM-1浓度与OI相关性好。经Logistic回归分析,入组第1天的血浆ESM-1与CRP、IL-6为预测脓毒症患者ARDS发生的独立危险因素,ESM-1的AUC为0.750,cut-off为5.865ng/mL,CRP的AUC为0.736,IL-6的AUC为0.736。结论 第1天的血浆ESM-1与CRP、IL-6为脓毒症患者ARDS发生的独立危险因素,血浆ESM-1作为脓毒症ARDS的预测标记物,有较好的价值。     

小儿鼾症手术前后血浆生物标志物的变化及临床意义

探讨小儿鼾症患者行扁桃体和（或）腺样体手术对与心血管疾病密切相关的血浆生物标志物的影响。选取单纯非肥胖型小儿鼾症患者20例,进行术前和术后6个月血浆超敏 C 反应蛋白（hs-CRP）、肿瘤坏死因子α（TNF-α）、白介素6（IL-6）、脂联素和胎球蛋白 A 的水平的测定,结果显示术后6个月的血浆 hs-CRP、TNF-α、IL-6、脂联素的水平均明显低于相应的术前水平（P <0.001）,胎球蛋白 A 的水平均明显高于相应的术前水平（P <0.001）。小儿鼾症患者进行扁桃体和（或）腺样体切除术有助于降低其并发心血管疾病的风险。     

血清生物标志物对纤维化性间质性肺疾病预后的预测价值

目的探讨与肺泡上皮细胞功能障碍相关的血清生物标志物对纤维化性间质性肺疾病(fibrosing interstitial lung diseases, FILD)预后的预测价值。方法前瞻性入组FILD患者,收集临床资料,检测生物标志物并随访1年进行病情评估。采用竞争风险模型的生存分析和Fine-Gray回归模型提取发展为进行性纤维化型和死亡风险的影响因素。结果共入组58例患者,35例诊断为特发性肺纤维化,14例诊断为结缔组织病相关间质性肺病,9例归为其他原因所致肺纤维化。12例(20.69%)患者发展为进行性纤维化型,8例(13.79%)死亡。单因素分析结果显示发展为进行性纤维化型的风险因素是糖蛋白抗原125(carbohydrate antigen-125,CA-125)基线增高,死亡的风险因素是糖蛋白抗原19-9(carbohydrate antigen 19-9,CA19-9)基线增高;多因素分析结果显示:CA19-9基线增高是死亡的风险因素(回归系数0.007,风险比1.007,95%CI:1.001～1.061,P=0.044)。结论 CA-125基线增高的患者发展为进行性纤维...     

Effect of Continuous Positive Airway Pressure and Upper Airway Surgery on Exhaled Breath Condensate and Serum Biomarkers in Patients With Sleep Apnea

IntroductionStudies on inflammation biomarkers in serum and in exhaled breath condensate (EBC) in obstructive sleep apnea (OSA) have shown conflicting results. The objective of this study is to assess EBC and serum biomarkers in OSA patients at baseline and after continuous positive airway pressure (CPAP) or upper airway surgery (UAS).Patients and methodsNine OSA patients referred for UAS were matched for anthropometric characteristics and apnea–hypopnea index with 20 patients receiving CPAP. pH, nitrite (NO₂⁻), nitrate, and interleukin 6 in EBC and NO₂⁻, nitrate, leukotriene B₄, and interleukin 6 in serum were determined. EBC and serum samples were collected at baseline and 3 months after CPAP or UAS.ResultsPatients’ mean body mass index was 30 (range 24.9–40) kg/m². EBC biomarker levels at baseline were within normal range and did not differ significantly after CPAP or UAS. No significant changes were observed in the serum concentration of the biomarkers determined after CPAP but the serum concentration of NO₂⁻ increased significantly at 3 months after UAS (P=.0078).ConclusionIn mildly obese OSA patients, EBC biomarkers of inflammation or oxidative stress were normal at baseline and remained unchanged 3 months after UAS or CPAP. Although UAS was not effective in terms of reducing OSA severity, it was associated with an increase in serum NO₂⁻.