精子DNA碎片率与体外受精实验室结局的关系

目的初步探讨精子DNA碎片率、精子核蛋白不成熟度与体外受精实验室结局的关系。方法对2013年5~7月在我院行IVF治疗的男性患者行精液常规、精子DNA碎片率及精子核蛋白不成熟度检查,利用spearman相关分析探讨它们与IVF受精率、优胚率的关系。结果精子DNA碎片碎与男方年龄正相关,与前向运动精子百分率、优胚率负相关,与IVF受精率无相关。精子核蛋白不成熟度与精子浓度、正常精子百分率负相关,与IVF受精率、优胚率无显著相关。结论精子DNA碎片率检测对预测IVF优胚率有一定的临床意义。     

多功能超高倍显微分析仪在不育症精液分析中的应用

目的探讨运用多功能超高倍显微分析仪直接湿片观察精子形态的应用价值。方法支原体感染不育症患者156份精液标本,用改良巴氏染色法,1000倍油镜观察精子形态,同时运用多功能超高倍显微镜直接湿片观察精子形态。结果两种方法对精子形态的评估经统计学分析,正常形态率和各种类型缺陷率差异均无统计学意义（P〉0．05）．结论运用多功能超高倍显微分析仪直接观察精子形态是一种简单、快速、有效的方法,值得推广。     

不育厨师精液质量参数及精子DNA完整性分析

目的探讨不育厨师精液常规质量参数和精子畸形率、精子DNA碎片率等指标。方法对生殖门诊52名不育厨师（观察组）和49名育前体检者（对照）行精液常规和DNA碎片率等检测。结果观察组精液液化异常率、精子畸形率、DNA碎片率都显著高于对照组;精子浓度、前向运动精子比率显著低于对照组。结论厨师职业暴露会影响精液质量,导致男性不育,应加强保护和防范。     

Sperm DNA damage in patients with chronic viral C hepatitis

Introduction

The aim of this study was to evaluate the conventional and biofunctional parameters of sperm in young infertile patients with Hepatitis C (HCV) infection.

Methods

Forty HCV patients with primary infertility, aged 27 to 42 years (mean 36.4 years) and twenty HCV patients with secondary infertility aged 28 to 45 years (mean 35.0 ± 2.8 years), underwent hormonal and sperm analysis in addition to the determination of reactive oxygen species (ROS) concentrations in the sperm and flow-cytometric evaluation. The following biofunctional sperm parameters were evaluated by flow cytometry: DNA fragmentation, mitochondrial membrane potential, chromatin condensation, and the rate of early apoptosis.

Results

Overall, patients with HCV showed significantly worse median values of conventional and biofunctional sperm parameters than control subjects, including sperm density (31.7 vs. 80.4 million/ml), forward motility (9.4 vs. 25%), normal forms (15.4 vs. 24.8%), DNA fragmentation (6.6 vs. 2.2%), low MMP (45.5 vs. 8%), an early apoptosis rate (5 vs. 2.7%), and abnormal chromatin (18.9 vs. 13.9%). Finally, HCV patients had significantly higher basal (250 vs. 75 × 10³/cpm) and stimulated (550 vs. 120 × 10³/cpm) ROS levels in semen compared to control subjects. None of the examined parameters (sperm, hormonal, biofunctional and assessment of oxidative status in the semen) was significantly different between HCV patients with primary and secondary infertilities.

Discussion

These results confirm that HCV infection has a negative impact on sperm parameters. The overlap of the results observed in the two groups of HCV patients supports the hypothesis that HCV infection may cause to alterations in sperm parameters.     

精子尾部卷曲试验与精子低渗肿胀试验的共性与特性

探讨了精子尾部卷曲试验与精子低渗肿胀试验的关系。两种实验原理相同，实验条件（渗量、离子浓度）不同，实验结果存在一定差异。对９５例生育和不育男性精液检查结果表明：精子卷尾率与精子尾总肿胀率呈高度正相关（ｒ＝０．９１２）；精子卷尾率与精子低渗肿胀试验ｇ型精子率比较呈极显著差异（Ｐ＜０．００１）；精子卷尾率、精子低渗总肿胀率与精子活率呈高度正相关（ｒ分别为０．９６３和０．９３２）；生育组与不育组比较精子卷尾率、精子总肿胀率及ｇ型精子率均呈显著性差异（Ｐ＜０．０１）。采用精子卷尾率结合低渗肿胀试验ｇ型精子率综合评估精子的活动功能对男性不育症的实验诊断具有良好的应用价值。     

精子DNA碎片率对男性生育力评估的预测价值

目的：探讨精子DNA碎片率（DFI）对男性生育力评估方面的预测价值。方法：选择2016年1月-2018年7月于上海市第十人民医院生殖医学中心就诊的460例男性不育患者纳入观察组,同期107例正常生育男性纳入对照组。计算机辅助精液分析系统（CASA）检测精液常规,精子染色质结构分析检测精子DFI。将精液常规结果分为精液正常、少精子、弱精子和少弱精4类。比较观察组和对照组的精液常规构成比和精子DFI;比较不同年龄（＜30岁,30～39岁,≥40岁）患者精子DFI水平的变化趋势及不同水平精子DFI（≤15%,15%～30%,≥30%）患者的体外受精（IVF）/胞浆内单精子注射（ICSI）的生殖结局。结果：观察组中精液正常、少精子、弱精子、少弱精所占比列分别为22.17%、13.26%、52.62%和11.95%,对照组分别为60.96%、34.59%、1.75%和2.70%。与对照组相比,观察组精液正常比例下降,少精子、弱精子和少弱精比例增加,差异有统计学意义（P＜0.05）。与对照组相比,观察组精子DFI≤15%所占比例下降,DFI≥30%比例增加,差异有统计学意义（P＜0.05）。随着年龄增加,精子DFI≤15%患者所占比例下降,精子DFI（15%～30%）与精子DFI≥30%患者所占比例增加,3组间精子DFI的分布比例差异有统计学意义（P＜0.05）。精子DFI≥30%来源的IVF或ICSI胚胎,其早期流产率显著升高（31%和25%,均P＜0.05）,但受精率、卵裂率、生化妊娠率、临床妊娠率、着床率等指标差异均无统计学意义（P＞0.05）。结论：不育男性的精子DNA碎片率更高,并且随年龄的增加而增加。DFI≥30%的精子受精的胚胎早期流产率较高,影响辅助生殖技术的妊娠结局,因此DFI对不育男性的生育力评估有实用参考价值。     

Protective effect of MOTILIPERM in varicocele-induced oxidative injury in rat testis by activating phosphorylated inositol requiring kinase 1α (p-IRE1α) and phosphorylated c-Jun N-terminal kinase (p-JNK) pathways

Context: MOTILIPERM was prepared as a mixture of extracts of three medicinal herbs [roots of Morinda officinalis How (Rubiaceae), outer scales of Allium cepa L. (Liliaceae) and seeds of Cuscuta chinensis Lamark (Convolvulaceae)].

Objective: To investigate the role of reactive oxygen species (ROS)-based endoplasmic reticulum (ER) stress in a rat model of varicocele and the therapeutic efficacy of MOTILIPERM in this model.

Materials and methods: Sixty male rats were divided into five experimental groups: a normal control group (CTR?+?vehicle), a control group administered MOTILIPERM 200?mg/kg (CTR?+?M 200), a varicocele-induced control group (VC?+?vehicle) and two varicocele-induced groups administered MOTILIPERM 100 (VC?+?M 100) or 200 (VC?+?M 200) mg/kg for 4 weeks. Testis weights were recorded and serums were assayed for hormone concentrations. Tissues were subjected to semen analysis, histopathology, analyses of ER response protein expression levels and oxidative stress were assessed by measuring ROS, reactive nitrogen species (RNS), malondialdehyde (MDA) level and ratios of total glutathione (GSH)/oxidized GSH (GSSG).

Results: MOTILIPERM treatment of varicocele-induced groups significantly increased left testis weight, testosterone level, sperm motility, count and spermatogenic cell density. ER-response protein expression levels were dose-dependently decreased in VC?+?M 200 group compared with VC?+?vehicle group. MOTILIPERM treatment also decreased MDA and ROS/RNS level but increased GSH/GSSG ratio.

Discussion and conclusions: This study suggests that ROS-related ER stress may play a major role in varicocele-induced infertility and MOTILIPERM, a novel compound targeting ROS-based ER stress, may be therapeutically useful in treatment of varicocele, or as a supplement for the treatment of infertility.     

加味大黄?虫颗粒对精索静脉曲张模型大鼠精子线粒体结构的影响

目的:探索加味大黄?虫颗粒对精索静脉曲张模型大鼠精子线粒体结构的影响。方法:40只雄性大鼠随机选出10只作为假手术组,剩余30按照Turner方法制造左侧精索静脉曲张模型大鼠,造模完成后,按照随机数字表法分为加味大黄?虫颗粒组、模型组、迈之灵组,每组各10只。加味大黄?虫颗粒组给予加味大黄?虫颗粒灌胃; 迈之灵组予迈之灵片,假手术组和模型组分别予等剂量0.9%生理盐水灌胃。8周后处死所有大鼠,取出左侧睾丸组织,完成精液质量分析、睾丸组织病理检测。结果:灌胃8周后,加味大黄?虫颗粒组精子浓度、总活率、前向运动精子、精子直线速度(VSL)、精子曲线速度(VCL)、平均路径速度(VAP)、精子头侧摆幅度(ALH),明显高于模型组(P<0.05)。模型组睾丸组织病理报告生精小管管腔空化,各级生精细胞破坏严重,精子膜结构破坏,精子线粒体肿胀、溶解。而加味大黄?虫颗粒组中睾丸组织的病理损伤得到不同程度的改善,精子线粒体结构完整,形态大小规则。结论:加味大黄?虫颗粒在一定程度上修复精索静脉曲张大鼠睾丸组织的病理损伤,进而起到保护精子线粒体结构与功能的作用,为精子的发育、活动提供稳定的能量代谢环境,有助提高精子活力及增加受精能力。     

卵子激活剂对无精子症患者冻融睾丸精子ICSI后妊娠结局的影响

目的研究卵子激活剂CultActive在临床中能否提高无精子症患者冻融睾丸精子行卵胞浆内单精子显微注射(ICSI)后的妊娠结局。方法选择2015年1月~2019年12月我院收治的188例行冻融睾丸精子ICSI助孕治疗的无精子症患者作为研究对象,按照患者ICSI后是否进行应用卵子激活剂CultActive随机分为冻融睾丸精子组(n=107)和卵子激活组(n=81),对照组为新鲜睾丸精子组(n=129),分别比较了三组患者在年龄、不孕年限、促性腺激素用量、促性腺激素使用天数等临床资料的差异以及获卵数、受精率、2PN率、分裂率、优胚率、可利用胚胎率、临床妊娠率等胚胎发育情况的差异。结果三组患者在年龄、不孕年限、促性腺激素用量、促性腺激素使用天数、获卵数上均无统计学差异(P>0.05)。B组在2PN率、优胚率、可利用胚胎率上均高于A、C组,但无统计学差异(P>0.05)。三组患者在分裂率上也无统计学差异(P>0.05)。B组在受精率上明显高于C组(P<0.05),且显著高于A组(p<0.01)。B组在临床妊娠率上显著高于A、C组(p<0.01)。结论卵子激活剂CultActive可以提高冻融睾丸精子ICSI效率,值得在临床中推广。