MicroRNA-543 in Systemic Diseases and Possible Applications in Gynecologic Tumors

In recent years, an increasing number of young women have been diagnosed with cancer, including some nulliparous women. Therefore, many young patients with earl...     

宫腔镜对子宫内膜质量和试管婴儿成功率的影响

目的探讨宫腔镜对子宫内膜质量与容受性的相关性及对试管婴儿成功率的影响。方法选取我院2017年6月～2018年3月期间收治的不孕患者80例。将其随机分成两组,即研究组和对照组。其中,研究组的患者将采用宫腔镜子宫内膜微创术进行干预治疗;对照组患者予基础干预治疗。对比两组患者的干预后各指标的结果,进而对比两组的干预效果。结果研究组患者的孕激素表达水平明显高于对照组(P0.05);研究组患者的血清性激素略高于对照组(P0.05);研究组患者试管婴儿的成功率明显高于对照组(P0.05)。结论对不孕的患者而言,采取使用宫腔镜子宫内膜微创术的干预治疗方式,可以有效改善患者子宫内膜的环境,促进患者孕激素以及患者血清性激素的分泌,能有效提升患者进行试管婴儿的成功率。     

卵泡晚期均质子宫内膜的容受性研究

目的探讨卵泡晚期超声下均质回声子宫内膜的容受性。方法收集卵泡晚期超声下均质子宫内膜妇女14例（实验组）,分别于排卵前和黄体中期取子宫内膜组织,测定白血病抑制因子（LIF）、整合素αvβ3、基质金属蛋白酶-9（MMP9）及血管内皮生长因子（VEGF）水平,其结果与14例卵泡晚期三线征回声内膜妇女（对照组）相比较。结果卵泡晚期对照组和实验组子宫内膜腺体VEGF水平分别为1.39±0.46和0.96±0.37,差别有显著性（P=0.010）。对照组平均LIF、整合素αv、β3水平分别为2.59±0.61、2.60±0.73和2.83±0.57,均高于实验组的1.58±.99、1.85±0.72和1.92±0.91,差别有统计学意义（P=0.003、P=0.011和P=0.004）。结论卵泡晚期均质内膜妇女的子宫内膜容受性低于三线征内膜妇女。     

补肾健脾活血法对超排卵小鼠种植窗期子宫内膜LIF表达的影响

目的:观察补肾健脾活血法对超排卵小鼠子宫内膜白血病抑制因子(LIF)表达的影响。方法:雌性小鼠予阿拉瑞林/孕马血清促性腺激素/人绒毛膜促性腺激素构建超排卵小鼠模型,并随机分成实验组(中药组)、阳性对照组(阿司匹林组)、阴性对照组,分别给予补肾健脾活血中药、阿司匹林、0.9%氯化钠注射液进行干预。在小鼠种植窗期取子宫中段组织,用逆转录-PCR(RT-PCR)法检测小鼠子宫内膜组织中LIF mRNA的表达水平。结果:中药组与阿司匹林组超排卵小鼠子宫内膜LIF mRNA的表达均明显高于阴性对照组,差异有显著统计学意义(P<0.01);中药组LIF mRNA的表达高于阿司匹林组,差异有统计学意义(P<0.05)。结论:补肾健脾活血法可以提高超排卵状态下子宫内膜LIF mRNA的表达水平,改善超排卵状态下子宫内膜的容受性,效果优于阿司匹林。     

氧化应激对子宫内膜异位症患者在位内膜容受性的影响

目的:探讨氧化应激(OS)对子宫内膜异位症(EMs)患者在位内膜容受性的影响。方法:采用黄嘌呤氧化酶法及硫代巴比妥酸比色法分别检测60例EMs患者(EMs组)在位内膜(增殖期24例,分泌期36例)和50例正常内膜(对照组)(增殖期21例,分泌期29例)中超氧化物歧化酶(SOD)和丙二醛(MDA)水平。采用同种方法检测对照组内种植窗期内膜17例(A组),EMs组经治疗后口服维生素C(Vc)抗氧化治疗患者恢复正常月经后第1个月种植窗期内膜12例(B组),EMs组经治疗后患者恢复正常月经后第1个月种植窗期内膜11例(C组)和EMs组内种植窗期内膜23例(D组)中SOD和MDA水平。采用免疫组化法检测A、B、C、D组内膜整合素αvβ3表达情况。分析A、B、C、D组内膜整合素αvβ3表达与SOD、MDA水平相关性。结果:无论增殖期还是分泌期内膜,EMs组的SOD水平均低于对照组(P0.05),MDA水平均高于对照组(P0.05)。4组种植窗期内膜中,SOD水平为A组B组C组D组,MDA水平为A组B组C组D组,αvβ3水平为A组B组C组D组;B、C、D组中,内膜整合素αvβ3表达与SOD水平均呈正相关(P0.05),与MDA水平均呈负相关(P0.05)。结论:EMs患者在位内膜存在OS,其降低在位内膜容受性;治疗后的EMs患者可明显改善在位内膜OS状态,进而改善在位内膜容受性,抗氧化治疗可以起到辅助作用。     

Cytometric assessment of uterine receptivity via epithelial β3 integrin expression

Research questionMany techniques now exist to assess the receptivity status of the endometrium. Can a simple low-cost flow cytometric technique be used to rapidly assess uterine receptivity via a luteal phase endometrial biopsy?DesignEpithelial β3 integrin (EB3) evaluation was undertaken in 300 women presenting with repeated implantation failure or recurrent pregnancy loss who subsequently underwent 710 assisted reproductive technology (ART) cycles. Endometrial tissue was mechanically dissociated and evaluated using specific antibodies to capture integrin expression. Autologous and donor oocyte embryo transfers were evaluated. A ‘High’, ‘Borderline’ and ‘Low’ grading system was developed based on the pattern and percentage expression of EB3 relative to the total endometrial epithelium. Clinical outcomes of the resulting embryo transfers (n = 559) were analysed according to EB3 grading.ResultsBased on 180 completed transfers, the clinical pregnancy rate (CPR) per embryo transferred in the donor egg cycles was 41.7%. EB3 results from women with a ‘High’ grading showed a superior CPR (54.0%) compared with ‘Low’ (22.2% CPR) or ‘Borderline’ (37.4%) cases (P = 0.024). Similarly, following 379 autologous oocyte transfers, the CPR was 36.1% overall, with major variations between the ‘High’ (43.8%), ‘Low’ (17.5%) and ‘Borderline’ (34.8%) groups (P = 0.0008). Implantation rates showed similar significant trends in the ‘High’ versus ‘Low’ groups of 40.4% versus 16% (P = 0.048) in donor oocyte transfers, and 30.8% versus 16.1% (P = 0.025) in autologous oocyte transfers.ConclusionsThe distribution patterns and percentage expression of EB3 assessed by a flow cytometry grading system shows a significant relationship to implantation rate and CPR success in ART cycles and may thus represent a useful additional tool for the assessment of uterine receptivity.     

中医药对子宫内膜容受性调节作用的研究进展

医学研究者在调节子宫内膜容受性方面做了大量积极探索,临床研究和动物实验均表明中医药对调节子宫内膜容受性具有传统优势,本文主要综述了近年来中医药调节子宫内膜容受性的临床和实验研究进展.     

埃兹蛋白在不同时期人子宫内膜的表达

目的:探讨埃兹蛋白在不同时期人子宫内膜表达的变化规律.方法:采用免疫组织化学方法检测人正常月经周期不同时期的子宫内膜组织中埃兹蛋白的表达.结果:埃兹蛋白在人月经周期各期子宫内膜均有表达,以分泌中晚期子宫内膜组织中表达较强,表达量明显高于增生期和分泌早期(P＜0.05).结论:埃兹蛋白在正常子宫内膜中的表达呈现明显周期依赖性,分泌中晚期表达增强提示可能与种植窗的开放相关,从而参与子宫内膜容受性的建立.     

The role of reinforcement in the sexual behavior of the female rat

In order to clarify the role of reinforcement in the sexual behavior of female rats, females were trained to traverse a runway to achieve contact with incentive males. Females learned to run faster to sexually active males that achieved intromission than to passive males (p<0.05). However, both types of incentive males produced significant learning (p< 0.001). The type of estrus, either natural or hormone-induced, did not affect learning measures. The results were interpreted as requiring that an explanatory model of the female's sexual behavior contain a variable accounting for reinforcement specific to sexual contact as well as for the more general social reinforcement and the well established aversive consequences of mating.     

健胎液对模型小鼠着床期子宫内膜容受性的影响

目的:研究健胎液对胚泡着床障碍小鼠着床期子宫内膜容受性的影响。方法:于妊娠第4天9∶00利用米非司酮建立胚泡着床障碍小鼠模型,予健胎液进行干预,于妊娠第4天21∶00处死小鼠,观察小鼠胚泡着床率及平均着床胚泡数,采用放免法检测血清及子宫组织雌二醇、孕酮的含量,采用逆转录聚合酶链式反应(RT-PCR)检测子宫ERmRNA、PRmRNA表达。结果:与模型组相比,中药组着床率和平均着床胚胎数均显著升高,与对照组无显著差异;模型组子宫内膜发育不良,中药组子宫内膜的发育与正常组相似;3组血清及子宫组织的雌二醇和孕酮的含量无显著改变;中药组子宫ER mRNA、PR mRNA表达均较模型组显著提高,与正常组比较差异无显著性。结论:健胎液通过促进子宫内膜发育,调节胚泡着床障碍小鼠子宫组织ER、PR基因的表达,改善子宫内膜容受性,利于胚泡着床。