滚轮微针联合胶原蛋白敷料改善面部光老化的自身半脸对照研究

目的 探讨滚轮微针联合胶原蛋白敷料改善面部光老化的临床疗效及安全性.方法 将26例符合标准的受试者两侧面部配对并随机分为两组,对照组单纯使用胶原蛋白敷料治疗,治疗组采用滚轮微针联合胶原蛋白敷料.共治疗3个疗程,每次间隔2周.结果 GAIS评分结果显示:在纹理、毛孔、皱纹改善评价中,治疗组改善率分别为94.4％、94.4...     

A pilot investigation to subjectively measure treatment effect and side-effect profile of non-ablative skin remodeling using a 532 nm, 2 ms pulse-duration laser

BACKGROUND AND OBJECTIVE : Carbon dioxide laser skin resurfacing has become a standard treatment for wrinkles and sun-damaged skin. This ablative treatment, however, is associated with undesirable complications and long recovery times. A growing body of evidence suggests that dermal inflammation and subsequent collagen formation can be stimulated without removal of the epidermis, raising the possibility of effective non-ablative skin remodeling for mild to moderately photodamaged skin. MATERIALS AND METHODS : This preliminary study was performed to evaluate the safety and subject satisfaction of non-ablative skin remodeling using a 532 nm, 2 ms pulse-duration, frequency-doubled Nd:YAG laser. Subjects with mild-to-deep lip wrinkles and mild-moderate acne scarring were treated one half of their lip (wrinkles) or cheek (acne scarring), leaving the other side as an untreated control. Subjects were treated at 3-6 week intervals for an average of three treatments. Subjective assessment of improvement was estimated by subject self-evaluation of the percentage improvement over baseline, and a blinded observer attempted to identify the treated side on physical examination. RESULTS : Subjective assessment revealed an average improvement of 51.4% and 53.6% for upper lip wrinkles and facial acne scarring, respectively. Side effects were limited to transient erythema that resolved over 0.25-2 hours following treatment. CONCLUSIONS : These results demonstrate that non-ablative treatment with the 532 nm, 2 ms pulse-duration Nd:YAG laser results in subjective improvement of rhytides and acne scarring, with a high safety profile.     

激光和强脉冲光对小型猪皮肤光老化的改善性治疗研究

目的建立可模拟临床皮肤激光研究的光老化实验动物模型,观察不同类型的非剥脱激光和强脉冲光对模型动物皮肤光老化的改善性治疗作用。方法分别应用强脉冲光,长脉宽1064 nm Nd:YAG激光,1320 nm Nd:YAG激光对光老化区域皮肤进行照射治疗2次,分别在激光治疗前、第一次激光治疗后2周、第二次激光治疗后4周,检测皮肤生理功能并行组织活检,观察皮肤真皮胶原的改变和皮肤羟脯氨酸含量的变化。结果与正常皮肤比较,紫外线照射区皮肤含水量和皮肤弹性下降,黑色素指数增加,均有统计学差异(P0.05);经激光和强脉冲光治疗后,与未经治疗的光老化皮肤区相比较,皮肤含水量和血红素含量无明显变化(P0.05),而皮肤弹性和羟脯氨酸含量均有明显改善,差异有统计学意义(P0.05);病理显示:激光和强脉冲光治疗后,真皮内胶原纤维增加。结论强脉冲光和非剥脱激光能够较快速地改善紫外线造成的皮肤光老化的一些表现,对表皮的损伤很小。     

中波紫外线对角质形成细胞MMP-1及TIMP-1的影响

目的：研究中波紫外线照射对永生化人角质形成细胞的影响。方法：绘制细胞生长曲线,用不同剂量UVB（30、60、90 mJ/cm2）照射永生化人角质形成细胞,用MTT方法测定UVB照射后细胞的增殖活性,用RT-PCR方法测定HaCaT细胞中MMP-1mRNA和TIMP-1mRNA的表达。结果：UVB照射后,HaCaT细胞的增殖活性受到抑制,MMP-1 mRNA表达增强,TIMP-1 mRNA表达下降,90 mJ/cm2照光组与未照光组比较,差异均有统计学意义。结论：UVB照射可诱导HaCaT细胞损伤和细胞凋亡,促使MMP-1mRNA表达增加,TIMP-1 mRNA表达减少,二者比例失调,这可能与光老化的发生有一定的关系。     

杜仲提取物抗UVA致ESF-1细胞光老化作用的机制研究

目的:探讨杜仲提取物(EE)抗UVA致人真皮成纤维细胞光老化的作用机制。方法:将体外培养的ESF-1细胞通过UVA照射建立细胞光老化模型,给予500μg/mL、250μg/mL、125μg/mL、62.5μg/mL 4种不同浓度的杜仲提取物,采用ELISA法检测细胞培养液中Ⅰ型胶原蛋白(ColⅠ)、基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-3(MMP-3)的含量。结果:ESF-1细胞接受40J/cm的UVA照射后,细胞培养液中MMP-1、MMP-3含量明显升高(P0.05),ColⅠ含量明显降低(P0.05);给予EE后,细胞培养液中ColⅠ含量明显升高(P0.05),MMP-1含量明显降低(P0.05),MMP-3含量无明显变化。结论:杜仲提取物能抑制UVA引起的人MMP-1及MMP-3的异常分泌,降低ColⅠ的降解。     

Glycolic acid and its use in dermatology

Glycolic acid has distinctive pharmacologic properties that recommend it for topical use in a range of skin disorders: acne, aging and photoaging, keratoses, age spots. Future studies and more extensive and varied clinical trials would surely point out its therapeutic potential and extend its range of clinical applications.     

茶色素对紫外线照射致小鼠皮肤光老化的防护效应

目的:研究茶色素对紫外线照射致小鼠皮肤光老化的防护效应。方法:用比色法测定小鼠皮肤组织的总抗氧化能力与脂质过氧化产物——丙二醛的含量。结果:灌胃和外涂茶色素均能明显提高经紫外线照射的小鼠皮肤组织总抗氧化能力(P<0.05),明显降低组织中丙二醛的含量(P<0.05)。结论:茶色素能防治紫外线照射引起的自由基损伤,减缓皮肤光老化。     

Effects of red ginseng extract on UVB irradiation-induced skin aging in hairless mice

Panax ginseng C.A. Meyer, Korean herb medicine, has been widely used in China and Japan for fatigue and enhancement of resistance to many diseases.