马拉色菌扩增DNA多态性研究

应用RAPD法对不同来源的马拉色菌进行DNA分型。结果发现47株马拉色菌临床分离株可分为6型,其DNA型别与孢子形态之间无绝然对应关系,不同地区的马拉色菌DNA带型之间似无明显差异。马拉色菌DNA型别与其所致临床病型之间无明显对应关系。但可能存着一定的优势关系。     

球形马拉色菌感染引起皮肤慢性穿掘性脓肿1例

患者男 ,17岁。临床表现为头面部、颈部、胸背部、肩部等部位多发性、大小不等的疼痛性红斑丘疹、红色硬性结节、脓疡及瘘管。从患者的皮肤组织分离并鉴定为球形马拉色菌。在脓皮病患者分离出球形马拉色菌尚属首次报告。氟康唑和伊曲康唑治疗本病效果显著。     

Psoriasis with a dirt‐adherent: A rare case report

Cutaneous dirt‐adherent disease (CDAD) is a rare psychogenic dermatosis mainly occurring in young Japanese and Chinese women. It mainly occurs on cheeks, forehead, nipple, mammary areola and around mammary areola. To our knowledge, this is the first case of CDAD with the skin lesion of psoriasis rupioides to be reported. In our case, the patient, a 43‐year‐old Chinese man presented with thick, yellowish‐brown adherent crusts on his face with severe painful 6‐days duration. Histopathologic image: Parakeratosis, the epidermis demonstrates regular acanthosis with some thinning of the suprapapillary plates, neutrophils exocytosis are noted. As for the histopathologic diagnosis in his right crus, combined with the clinical manifestation of rupioides‐shaped crusts, film phenomenon and Auspitz's sign, we considered Psoriasis rupioide.     

马拉色菌属RAPD基因分型与相关疾病的关系

目的 :探讨马拉色菌属在临床疾病中的意义。方法 :利用RAPD对临床分离株进行基因分型 ,建立马拉色菌属带型关系树状图 ,分析与相关疾病的对应关系。结果 :临床标本可分成 6类 ,其中 5类可进一步分为亚类。各类与疾病无明显的对应关系 ,但可得到具有临床意义的结果。结论 :RAPD作为研究马拉色菌属基因多态性的工具 ,马拉色菌属在相关疾病中存在多样性     

Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of Malassezia restricta

The genus Malassezia is associated with a wide range of skin diseases and is the predominant fungal genus isolated from human skin. Of the 14 Malassezia species identified, M. restricta is the most abundant fungal species found from both healthy and diseased skin. Emerging evidences have suggested that extracellular lipases of Malassezia play a critical role in its survival on the host skin surface. This study aimed to characterise the lipase 1 homologue (MrLip1) in M. restricta and to analyse its expression under different environmental conditions. The full sequence of the gene encoding MrLip1 was determined by rapid amplification of cDNA ends, and it was then heterologously expressed in Pichia pastoris. MrLip1 protein was successfully purified and used for lipase assay and specific antibody generation for use in expression analysis. The optimum pH and temperature for the activity of purified MrLip1 were pH 5.0 and 34 °C respectively. Furthermore, the expression of MrLip1 peaked at a similar pH and temperature, suggesting that the optimal conditions for MrLip1 protein activity and expression are similar to that found on the human skin surface. This study provides data to improve our understanding of the role and characteristics of lipase 1 in M. restricta.     

3种中草药单体治疗豚鼠皮肤马拉色菌感染的实验研究

目的:筛选出有效的抗马拉色菌的中草药单体.方法:采用连续7 d涂菌法诱导豚鼠皮肤感染马拉色菌,并将苦参碱、柠檬醛、丁香酚3种中草药单体参考品及酮康唑粉制成2%凡士林霜剂,采用自身对照法观察治疗后培养皿中的真菌菌落数、真菌转阴率.结果:连续用药4 d后,柠檬醛、丁香酚组菌落数明显少于阴性及空白对照组,并且柠檬醛组真菌转阴率与酮康唑组差异无统计学意义;连续用药7d后,3种实验药物组的菌落数及真菌转阴率均明显低于阴性对照组及空白对照组,而与酮康唑组差异无统计学意义.结论:苦参碱、柠檬醛、丁香酚在体内具有较强的抗马拉色菌作用.皮肤菌落计数是定量指标,更能反应药物的短期疗效.     

Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tertiary Care Hospital,Punjab

Purpose: Pityriasis versicolor (PV) is a chronic superficial fungal disease caused by Malassezia species. Our aim was to identify Malassezia species from PV patients and healthy individuals in Punjab. Materials and Methods: Modified Dixon agar was used as isolation culture medium. Identification was based on morphological observation and biochemical evaluation. The biochemical evaluation consisted of culture onto Sabouraud dextrose agar, catalase reaction, Tween assimilation, Cremophor EL assimilation, splitting of esculin and growth at 38⁰C. Results: Out of 58 microscopically diagnosed cases of PV, growth was obtained from 54 (93.10%) cases. The most frequently isolated species were M. globosa, M. sympodialis and M. furfur which made up 51.79%, 31.42% and 18.51% of the isolated etiological agents respectively. However, the major isolate from the back of healthy individuals was M. sympodialis (47.61%), followed by M.obtusa (19.04%), M. globosa (14.20%), M. furfur (9.52%), M. pachydermatis (4.76%) and M. slooffiae (4.76%). Conclusions: M. globosa in its mycelial phase was the main etiological agent, but as normal flora from the back of healthy subjects, it was found in significantly less number (P = 0.01), suggesting that the higher pathogenicity of M. globosa in terms of enzymatic endowment, might be the cause of its predominance in PV lesions.