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81.
Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests. 总被引:1,自引:1,他引:1 下载免费PDF全文
C H Carbonetto E L Malchiodi M Chiaramonte E Durante de Isola C A Fossati R A Margni 《Clinical and experimental immunology》1990,82(1):93-96
By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas' disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes. 相似文献
82.
目的:测量各种小鼠呼吸模型的肺功能,尤其是非离体的动态肺功能,对于呼吸疾病的研究具有重要意义。 方法:主要包括整体测量方法和离体测量方法。而整体法又主要分为有创法(主要为气道阻力、顺应性的测定)和无创法(Penh,enhanced pause的测定)。本篇主要介绍整体的测量方法。 结论:不同的方法各有利弊,适用于不同的实验要求。 相似文献
83.
Preferential apoptosis of CD56dim natural killer cell subset in patients with cancer 总被引:5,自引:0,他引:5
Bauernhofer T Kuss I Henderson B Baum AS Whiteside TL 《European journal of immunology》2003,33(1):119-124
Natural killer (NK) cells (CD56(+)/CD3(-)) in the circulation of cancer patients were reported to have low NK activity and undergo spontaneous apoptosis. A possible relationship between apoptosis and impaired NK activity was studied by Annexin V-binding and NK-cell assays performed with peripheral blood mononuclear cells of patients with head and neck cancer (HNC), breast cancer (BC) and normal controls (NC). Cells stained with Annexin V (Anx) and antibodies to CD56, CD3, CD95, CD25, CD122 or CD132 were examined by flow cytometry. NK activity was tested against K562 targets in 4-h (51)Cr-release assays. The ratio of CD56(dim)/CD56(bright) NK cells was significantly different in patients vs. controls (10 vs. 16; p<0.01). A significantly greater percentage of CD56(dim) NK cells bound Anx in HNC patients (27+/-17%, median +/- SD) or BC (46+/-18%) than in NC (15+/-18%, p<0.04 and p<0.0002, respectively). CD56(dim) NK cells were preferentially targeted for apoptosis. NK activity was significantly lower in patients with HNC and BC than in NC (p<0.009). An inverse correlation between NK activity and the percent of Anx(+)CD56(dim) NK cells was observed in cancer patients (p =0.002) but not in NC. In patients, circulating CD56(dim) NK cells were targeted for apoptosis, leading to low levels of NK activity. 相似文献
84.
J. M. Vega M. Blanca J. J. Garcia M. J. Carmona A. Miranda M. Perez-Estrada S. Fernandez J. M. Acebes S. Terrados 《Allergy》1994,49(5):317-322
A large group of patients with suspected allergic reactions to (β-lactam antibiotics was evaluated. A detailed clinical history, together with skin tests, RAST (radioallergosorbent test), and controlled challenge tests, was used to establish whether patients allergic to β-lactam antibiotics had selective immediate allergic responses to amoxicillin (AX) or were cross-reacting with other penicillin derivatives. Skin tests were performed with benzylpenicilloyl-poly-L-lysine (BPO-PLL), benzylpenicilloate, benzylpenicillin (PG), ampicillin (AMP), and AX. RAST for BPO-PLL and AX-PLL was done. When both skin test and RAST for BPO were negative, single-blind, placebo-controlled challenge tests were done to ensure tolerance of PG or sensitivity to AX. A total of 177 patients were diagnosed as allergic to β-lactam antibiotics. We selected the 54 (30.5%) cases of immediate AX allergy with good tolerance of PG. Anaphylaxis was seen in 37 patients (69%), the other 17 (31%) having urticaria and/or angioedema. All the patients were skin test negative to BPO; 49 of 51 (96%) were also negative to MDM, and 44 of 46 (96%) to PG. Skin tests with AX were positive in 34 (63%) patients. RAST was positive for AX in 22 patients (41%) and to BPO in just 5 (9%). None of the sera with negative RAST for AX were positive to BPO. Challenge tests with AX were performed in 23 subjects (43%) to establish the diagnosis of immediate allergic reaction to AX, and in 15 cases (28%) both skin test and RAST for AX were negative. PG was well tolerated by all 54 patients. We describe the largest group of AX-allergic patients who have tolerated PG reported so far. Diagnosis of these patients can be achieved only if specific AX-related reagents are employed. Further studies are necessary to determine the exact extent of this problem and to improve the efficacy of diagnostic methods. 相似文献
85.
Varicocele: effect on sperm functions 总被引:2,自引:0,他引:2
Despite the numerous studies published over the past decade, the role of varicocele in male infertility is still controversial. Although more frequent in infertile men, its influence on sperm production or function has not, as yet, been determined. Moreover, the exact mechanism of varicocele action is not clear. We have surveyed the literature, the correlation of varicocele to sperm parameters and to sperm function tests, such as binding capacity, hypo-osmotic swelling test, presence of reactive oxygen species, and in particular, the correlation to fertility potential. Almost every subject examined had contradictory results. Larger control studies may possibly elucidate and clarify the cases in which varicocele is associated to sperm function, and where treatment may improve fertility. 相似文献
86.
Dendritic cells (DC) and natural killer (NK) cells, the main cellular components of the innate immune system, participate
in the most ancient first line of defense against infections. Both types of cells patrol peripheral tissues, whereas their
rapid recruitment and activation at mucosal surfaces [the major entry point for the human immunodeficiency virus (HIV)] is
a hallmark of acute inflammatory response. The ability of HIV to survive and replicate in the human host relies upon several
molecular mechanisms eluding the immune surveillance of both adaptive immunity and of DC and NK cells beginning with the acute
phase of primary HIV infection. DC and NK cells, unlike CD4+ T cells, are impaired more functionally rather than being depleted by HIV infection.
In this article we will review some of the aspects of DC/NK cells interaction with HIV infection both in vitro and in vivo,
and we will also speculate on the potential consequence for HIV pathogenesis and for the capacity of the virus to escape the
surveillance of the innate immune system. 相似文献
87.
We have investigated the density of peptides required to elicit different biological responses in cytotoxic T lymphocytes (CTL), including trogocytosis (i.e., the phenomenon whereby the lymphocytes actively capture fragments of plasma membrane from those cells with which they establish an immune synapse). We have used two separate mouse models of CTL recognising defined peptides presented by MHC class I molecules. In both systems, triggering of cytotoxicity and capture of membrane components reached saturation with low densities of ligand. On the other hand, down-modulation of cell-surface levels of TCR, induction of IFN-gamma production and detection of peptide captured required much higher ligand densities. Interestingly, fratricide (i.e., killing between CTL sharing the same specificity), a mechanism proposed to account for CTL exhaustion, was detected only at antigen concentrations still well above that second threshold leading to full blown activation. Taken together, our results show that the different thresholds that govern the elicitation of different CTL functions correlate with different proportions of antigen among the target cell components being captured via trogocytosis. 相似文献
88.
流式细胞术检测细胞毒方法的建立 总被引:7,自引:3,他引:7
目的:建立一种用流式细胞仪测定细胞介导细胞毒活性的方法。方法:用羧基荧光素二醋酸盐琥珀酰亚胺酯(CFSE)标记靶细胞,再用碘化丙碇(PI)标记DNA筛选细胞膜已破坏的靶细胞。效靶比为100:1—6.25:1,共孵育时间为1-6小时,流式细胞仪收集1000个靶细胞并测定被杀细胞的百分率。结果:CFSE是合适的标记靶细胞的荧光染料,18小时后也能很好地区分效靶细胞;靶细胞的自然死亡率低于5%;杀伤百分率随着效靶比和共孵育时间的增加而增加。最佳效靶比为50:1—25:1,共孵育时间为2—4小时。结论:CFSE/PI双荧光染料标记的流式细胞分析检测细胞毒具有多个优点:避免放射性试剂的应用,敏感性增强,单细胞水平的分析。 相似文献
89.
膜型/分泌型MICA对NK细胞受体NKG2D的相反调节效应及其对NK细胞受体谱的影响 总被引:21,自引:0,他引:21
目的 观察膜型和分泌型MICA对NK细胞受体表达的影响 ,以探讨NK细胞抗肿瘤活化机制及肿瘤细胞表达MICA分子的意义。方法 用MTT法测定人NK细胞系 (NK92 )的细胞毒活性 ;用RT PCR或FACS检测NK细胞受体 (NKG2D ,NKG2A B ,KIR2DL1,KIR2DS1)及NKG2D的识别配体MICA的表达。结果 肿瘤细胞表面的MICA分子可上调NKG2D的表达 ,下调抑制性受体NKG2A B和KIR2DL1的表达 ;而分泌型MICA (sMICA)分子对NKG2D及抑制性受体的表达均有抑制作用。结论 膜型MICA分子可上调NKG2D的表达 ,激发NK细胞对肿瘤细胞的细胞毒效应 ;分泌型MICA分子则通过降低NKG2D的表达下调机体的抗肿瘤免疫效应 ,肿瘤细胞分泌sMICA分子为肿瘤发生免疫逃逸的机制之一。 相似文献
90.
In the event of smallpox bioterrorism, widespread vaccination may be required. Vaccinia immune globulin (VIG) has been used to treat complications from the smallpox vaccine. While the potency of VIG was defined by its ability to neutralize intracellular mature virus, a second form of vaccinia called the extracellular enveloped virus (EEV) is critical for virus spread in the host. The B5R-protein is one of many EEV-specific proteins. Immunoprecipitation and ELISA revealed that VIG recognizes the B5R-protein. An EEV plaque-reduction assay using a recombinant vaccinia that lacks the majority of the extracellular domain of B5R showed that the ability of VIG to neutralize EEV is principally directed at B5R. In addition, absorbing out the anti-B5R antibody present in VIG through the addition of recombinant B5R protein abrogated VIG's ability to significantly neutralize wild-type EEV. This work demonstrates the prominent role of B5R as a target of EEV-neutralizing activity of human antibodies. 相似文献