Construction and identification of eukaryotic eukaryotic expression plasmid pcdna3.1-bace and its transient expression in cells

Objective： To generate eukaryotic expression vector of pcDNA3.1-BACE and obtain its transient expression in COS-7 cells and high expression in the neuroblastoma SK-N-SH cells. Methods： A 1503 bp cDNA fragment was amplified from the total RNA of human neuroblastoma by RT-PCR method and cloned into plasmid pcDNA3.1. The vector was identified by digestion with restriction enzymes BamHI and XhoI and sequenced by Sanger-dideoxy：mediated chain termination. The expression of BACE gene was detected by immunocytochemistry method. Results： The results showed that the cDNA fragment included 1503 bp total coding region. The recombinant eukaryotic cell expression vector of pcDNA3.1-BACE was constructed successfully, and the sequence of insert was identical to the published sequence. The COS-7 cells and the neuroblastoma SK-N-SH cells transfected with the pcDNA3.1-BACE plasmid expressed high level of BACE protein in cytoplasm. Conclusion： The recombinant plasmid pcDNA3.1-BACE can provide very useful tool for researching the mason of Alzheimer＇s disease and lays the important foundation for preventing the AD laterly.     

A rare association of synchronous intraductal carcinoma of the breast and invasive carcinoma of ectopic breast tissue of the vulva: case report and literature review

Abstract.   Intra M, Maggioni A, Sonzogni A, De Cicco C, Machado LS, Sagona A, Talakhadze N. A rare association of synchronous intraductal carcinoma of the breast and invasive carcinoma of ectopic breast tissue of the vulva: case report and literature review. Int J Gynecol Cancer 2006; 16(Suppl. 1): 428–433.
Only 17 cases of breast carcinoma arising in vulvar ectopic mammary tissue have been reported. We present a unique case of synchronous pure intraductal carcinoma of the breast (DCIS) and invasive carcinoma of ectopic breast tissue of the vulva. A 53-year-old woman presented with a 2-cm nodule in left labium major of the vulva. A surgical biopsy revealed an invasive carcinoma of ectopic mammary tissue. The mammography showed irregular microcalcifications of the right breast. The patient underwent left hemivulvectomy, bilateral inguinal sentinel lymph node biopsy, and radioguided breast resection (radioguided occult lesion localization) of the microcalcifications. The definitive histology revealed negative inguinal sentinel nodes, no further residual tumor in the vulva, and a high-grade (grade 3) DCIS in the breast. The synchronous occurrence of primary breast carcinoma and ectopic breast tissue carcinoma in the vulva is an extremely rare finding, only once previously being reported and leading to unsolved problems of differential diagnosis. The presence of a pure DCIS of the breast makes this case really unique, definitively confirming the independent primary origin of both mammary tumors. The inguinal sentinel node biopsy avoided a bilateral inguinal dissection.     

螺旋CT虚拟内诊镜在小儿气管支气管异物诊断中的应用

目的探讨螺旋CT虚拟内诊镜(CTVE)在小儿气管、支气管异物诊断上的应用价值。 方法回顾性分析东南大学医学院附属徐州医院1998年1月至2004年12月收治的121例术前经CTVE诊断为气管支气管异物，并行支气管镜探查术患儿的诊疗情况，并分析CTVE诊断在临床应用上的意义及诊断的阳性符合率。 结果121例经CTVE诊断并行支气管镜探查证实并取除异物者115例，诊断符合率9504%。 结论CTVE检查是目前无创非侵入性诊断小儿气管支气管异物的较理想方法。     

绒毛及合体滋养层细胞吲哚胺2,3-二氧化酶表达与流产的关系

目的:探讨人早孕绒毛组织吲哚胺2,3-二氧化酶(IDO)的表达与流产的关系。方法:RT-PCR测正常妊娠和难免流产绒毛组织及JAR细胞株IDOmRNA表达;免疫组化分析两组绒毛组织IDO蛋白质表达;Westernblot检测体外培养的合体滋养层细胞IDO蛋白质表达;高效液相色谱法检测细胞培养上清液中有无犬尿氨酸。结果:难免流产组绒毛组织IDOmRNA及蛋白质表达均低于正常组;JAR细胞株不表达IDOmRNA;合体滋养层细胞表达IDO蛋白质;合体滋养层细胞培养上清中有犬尿氨酸。结论:绒毛组织IDO正常表达是维持妊娠所必需;体外培养的人早孕绒毛合体滋养层细胞表达的IDO具有活性。     

Levonorgestrel-releasing intrauterine system vs. transcervical endometrial resection for dysfunctional uterine bleeding.

OBJECTIVE: To compare the acceptability, efficacy, adverse effects, and user satisfaction of a levonorgestrel intrauterine system (LNG-IUS) and transcervical resection of the endometrium (TCRE) for the treatment of dysfunctional uterine bleeding. METHOD: Of 50 women with a pictoral blood loss assessment chart (PBAC) score of 100 or greater, 25 had a LNG-IUS inserted (Mirena; Schering, Berlin, Germany) and 25 underwent TCRE. Procedure-related complications, PBAC score, hemoglobin levels, adverse effects, and rates of acceptability and satisfaction were recorded at 3-month intervals for a period of 12 months. RESULTS: At the end of 1 year there were a 97% and a 94% reduction in menstrual blood loss in the LNG-IUS and TCRE groups, respectively, and hemoglobin concentration had increased by 5.5% in the LNG-IUS group and 5.2% in the TCRE group. Adverse effects were similar in both groups except for systemic effects, which were seen only in the LNG-IUS group. Satisfaction rates were about 80% in the 2 groups. CONCLUSION: Both treatments were found to be equally effective but LNG-IUS placement requires less operator skill and entails no operative hazards, and the device provides effective contraception.     

选择性肠道去污对小儿MODS防治效果的临床观察

目的研究选择性肠道去污(SDD)对小儿MODS的防治效果。方法2004年4月~2005年6月在ICU住院的符合MODS诊断的患儿60名,随机分成常规治疗组M1组(30名),常规治疗 口服庆大治疗(SDD)M2组(30名),分析研究治疗前、后两组患儿的血清炎症介质的变化、并发症的发生率和病死率。结果M1组在ICU的平均住院时间为(14.68±4.8)d,M2组为(9.69±1.56)d,(P<0.05);M1组出现感染并发症的人数多于M2组;M2组患儿中性粒细胞计数在治疗期间下降更为明显;M1组患儿血清TNF浓度在治疗前、后没有显著变化,M2组血清TNF浓度在治疗后明显降低。结论选择性肠道去污的治疗能有效减轻并发症,缩短病程,能否降低病死率有待进一步探讨。     

义利关系的不同类型及其实质

义与利之间的关系，从不同的角度和层面考察有不同的类型范式。从内容上考察，可从物质利益与伦理道德的关系、个人利益与社会公共利益的关系、物质生活需要与精神生活需要的关系、志向动机与功利效果的关系等角度来予以分析。从形式上考察，其关系有义利同一型、义利对立型、义利相互包含型、义利相互交叉型等类型。从价值取向上考察，大体有重义轻利型、重利轻义型、义利俱轻型和义利并重型。了解这些不同的义利关系类型，有助于我们深入而全面地把握义利关系的不同涵蕴及其实质，进而选择合乎社会需要的义利观。社会主义义利观既反对封建主义的重义轻利，又反对资本主义的重利轻义。它在义利观上的基本价值取向是义利并重，义利统一。     

前臂内侧神经血管复合体岛状筋膜皮瓣的应用解剖

目的为前臂内侧神经血管复合体岛状筋膜皮瓣的应用提供解剖学基础。方法上肢标本18侧,显微剥离表皮组织,观察前臂内侧皮神经、贵要静脉及各自营养血管的起始、走行、分支、分布及与其它血管的吻合情况;新鲜整尸标本5具,动脉灌注显影剂,剥离体被组织于X光下摄影,分析皮动脉的分布及其与毗邻血管的关系。结果前臂内侧皮神经前支与贵要静脉伴行,两者周围分布着外径大于0.5 mm的营养动脉,平均3.4支,它们分别来自尺侧下副动脉、尺侧返动脉和尺动脉,营养动脉彼此吻合,形成动脉血管链。其中第一支位于肘横纹上21.25±6.33 mm,血管外径1.03±0.24 mm。结论可以在前臂内侧部切取神经血管复合体岛状筋膜皮瓣,供临床使用。     

芫花根总黄酮含药血清对小鼠细胞免疫功能的影响

目的建立小鼠血清中芫花根总黄酮(TFRD)各成分的HPLC测定方法,探讨TFRD含药血清对正常小鼠细胞免疫功能的影响。方法HPLC法测定小鼠单次ig给药后不同时间点血药浓度;M TT法检测多次ig给药后含药血清对正常小鼠脾淋巴细胞增殖、NK与LAK细胞杀伤活性和腹腔巨噬细胞的吞噬功能等细胞免疫功能的影响。结果单次ig TFRD后血药浓度在20~30 m in达到高峰;TFRD含药血清能促进脾淋巴细胞增殖,增加NK和LAK细胞杀伤活性,增强腹腔巨噬细胞的吞噬能力。结论TFRD含药血清能增强正常小鼠细胞免疫功能。     

重庆市三峡水库开县消落区居民营养知识、态度、行为调查

目的:了解重庆市三峡水库消落区居民营养知识、态度、行为的情况及相关影响因素,为今后针对消落区居民进行的营养健康教育提供参考依据。方法:用整群抽样的方法对开县消落区102户的居民(农村50户,城镇52户)进行入户问卷调查。结果:大多数开县消落区居民对铁、疾病(如糖尿病、高血压等)相关营养以及食物营养价值的知识较贫乏;营养知识的掌握程度与居民的文化程度、体质指数有关。而饮食与疾病关系、花钱预防疾病以及食物价格与营养价值关系的认知态度不是很理想;营养知识越多、居民地在城镇、家人有慢性病患者、Engel指数越低则居民营养态度越好。在行为上,消落区居民饮食一般比较咸,经常吃腌腊肉、咸菜,淘米次数比较多,仅有77·5%的居民平时关注营养宣传;营养知识越多、初中以上文化程度的居民对营养行为趋于科学合理。结论:开县消落区居民亟需营养健康教育,并可以通过营养知识—态度—行为模式对居民发挥作用,以增加科学营养知识,增强健康营养意识,优化膳食结构和养成科学合理的饮食行为习惯。