Serum Antibody Ig G and Ig M Titers for Opisthorchis felineus Correlate with Eggs in Faeces - a Comprehensive Study in Chuvash Republic,Russia

The Cholangiocarcinoma is a. The risk of development of cholangiocarcinoma, generally a rare type of a liver tumor, increases during infection of Opisthorchiasis. For this reason the timely detection of Opisthorchiasis is important for Cholangiocarcinoma prevention. There are many studies which concern the detection of pathogenesis of Opisthorchis viverrini infection but a little known about Opisthorchis felineus. In this study we investigate a correlation of the eggs which are found in a faeces and are comparable with a serum Ig G and Ig M antibody level that were detected with ELISA test in a large group of patients. The result is showing positive correlation between evidence of the Opisthorchis felineus eggs that were found in a faeces and antibody Ig G and Ig M level in a serum. Moreover the combination of two methods can improve the Opisthorchiasis diagnostic: the serum antibody and faeces investigation of eggs .     

Characterization and Application of a Lytic Phage D10 against Multidrug-Resistant Salmonella

Salmonella is a widely distributed foodborne pathogen that is a serious threat to human health. The accelerated development of drug resistance and the increased demand for natural foods invoke new biocontrol agents to limit contamination by multidrug-resistant (MDR) Salmonella strains. In this study, a lytic Salmonella phage named D10 was characterized at the biological and genomic levels. D10 possesses a short latent period (10 min) and a large burst size (163 PFU/cell), as well as adequate stability under a range of pH conditions and moderate thermal tolerance. D10 effectively lysed different MDR Salmonella serovars and repressed their dynamic growth in the medium. Genomic analysis disclosed that D10 is a new member of the Siphoviridae family and lacks the genes implicated in lysogeny, pathogenicity, or antibiotic resistance. A three-ingredient phage cocktail was then developed by mixing D10 with previously identified myovirus D1-2 and podovirus Pu20. The cocktail significantly reduced the count of MDR strains in liquid eggs, regardless of the temperature applied (4 and 25 °C). These results suggest that phage D10 is a promising tool to prevent food contamination by MDR Salmonella.     

Various peptides as substrates for aminopeptidases from salmon eggs during embryogenesis

Seven p-nitroaniline-modified peptides were used as substrates for studies of aminopeptidase and peptidase activities in eggs of lake salmon Salmo salar Sebago at different stages of embryogenesis. The formation of p-nitroaniline during peptide hydrolysis was measured in the incubation medium and in maturing eggs. Activities of all studied aminopeptidases and peptidases increased during the development of fertilized eggs and sharply increased before hatching. L-alanin-p-NA, L-pro-p-Na, L-Arg-p-Na, and L-Phe-p-NA were hydrolyzed most of all, while N-Glt-L-Phe-p-NA and Z-Gly-Pro-p-NA least of all. __________ Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 142, No. 9, pp. 286–288, September, 2006     

不同浓度的阿苯达唑体外抗短膜壳绦虫机理探讨

目的：为研究阿苯达唑对短膜壳绦虫及虫卵的杀灭机制。方法：应用形态学方法,观察比较不同浓度的阿苯达唑对短膜绦虫的体外杀伤作用。结果：48h各组虫体活力均明显减弱,72h高浓度组虫体均死亡,虫体随着药物浓度的升高和作用时间的延长,体表损伤愈加严重,组织结构破坏明显,实质区细胞出现核固缩,核碎裂现象,48h虫卵出现形态改变,数量明显减少。结论：表明阿苯达唑对短膜壳绦虫及虫卵均有较强的损伤作用。     

Application of an aldrin and dieldrin ELISA to the detection of pesticides in eggs

This paper details the results obtained when a number of egg samples, collected in Egypt from different races of chicken were analyzed for the presence of the organochlorine insecticides aldrin and dieldrin. A simple ELISA was used for the detection and quantification of aldrin and dieldrin. The test was modified for application in this high protein system. Pesticide was detected in 83–352% (14/17) of the samples at levels ranging from 0.006 to 0.7 ppm (0.006 to 0.7 μg ml^‐1). People eating eggs containing these amounts of pesticides which are above the World Health Organisation average daily intake levels would be at increased risk.     

CDC25B蛋白S149/S321位点突变对小鼠1-细胞期受精卵发育的影响

目的:研究小鼠CDC25B蛋白S149位点对小鼠1-细胞期受精卵发育的影响及S149位点与S321位点的关系。方法:构建pBSK-CDC25B-S149A和pBSK-CDC25B-S149A/S321A突变体,体外转录成mRNA;采用小鼠超排卵技术取G1期受精卵,显微注射CDC25B-WT-mRNA和突变CDC25B-S149A-mRNA、CDC25B-S149A/S321A-mRNA,观察其对受精卵发育、M期促进因子(MPF)活性及CDC2-pTyr15磷酸化状态的影响。结果:CDC25B-S149A-mRNA注射组受精卵卵裂率明显高于CDC25B-WT-mRNA注射组,MPF活性提前1 h达到高峰;而联合突变体CDC25B-S149A/S321A-mRNA注射组卵裂率又显著高于CDC25B-S149A-mRNA注射组。结论:在小鼠1-细胞期受精卵有丝分裂过程中,蛋白激酶A(PKA)对小鼠CDC25B蛋白S149位点的磷酸化修饰是控制受精卵G2/M转换的重要方式,并且S149位点与S321位点可能具有协同作用。     

经腹及经阴道彩色多普勒超声诊断早期输卵管异位妊娠比较

目的比较早期输卵管异位妊娠行经阴道及经腹彩超检测结果,探讨阴道彩色多普勒超声诊断价值。方法2008年1月～2011年1月经手术病理证实的早期输卵管妊娠患者70例,使用仪器为RT-3000、EUB-2000超声诊断仪,Acuson—Aspen彩色多普勒超声诊断仪,分别经腹部全面扫查后再经阴道进一步观察子宫腔及附件区肿块的细微结构及血流情况等。结果“输卵管环”直径1．5,3．4cm的圆形或椭圆形环,环壁为稍强回声,中心为无回声区,滋养层JOI流频谱阻力指数（RI）最高0．53,最低小于0．39,平均（0．43±0．07）。阴道超声诊断“输卵管环”阳性率97．14％、滋养层血流频谱阳性率92．86％、诊断符合率98．57％,均高于经腹壁超声的71．43％、60．00％、78．57％（P〈0．05）。结论经阴道超声的应用使早期输卵管妊娠的诊断率大为提高,可作为可靠的首选方法,值得推广应用。     

C57BL/6J小鼠冻融IVF原核胚用于Cas9显微注射探讨

目的探讨小鼠体外受精(IVF)冻融后用于Cas9注射的可行性。方法新鲜C57BL/6J小鼠卵子IVF后采用冻存管法(EFS20/40)冷冻原核胚(单细胞胚)和2细胞胚胎,次日复苏后培养。比较冻融原核胚和2细胞胚胎的回收率及成活率。选取部分冻融原核胚与新鲜原核胚同时进行Cas9和稀释液胞质注射并培养至2细胞,分别比较注射后的存活率及2细胞发育率。结果冻融后的B6小鼠原核胚的回收率为92.5%、成活率为92.8%,2细胞胚的回收率为90.5%、成活率95.8%,两组数据差异无显著性(P0.05)。新鲜原核胚Cas9注射后的存活率为92.7%,空白组注射组为97.5%,冻融原核胚Cas9注射后的存活率为82.6%,空白组为92%,冻融组Cas9注射与各组差异有显著性(P0.05),其它各组差异无显著性(P0.05)。注射后的2细胞胚发育率差异无显著性(P0.05)。结论冻融原核胚可用于Cas9显微注射。     

禽蛋中抗菌药物残留状况研究

摘要：畜禽养殖中抗菌药物的使用,导致其在畜禽体内残留带来食品安全问题。禽蛋是人类获取动物蛋白的重要来源之 一,禽蛋中蓄积的抗菌药物经食物链进入人体,威胁人类健康。目前,禽蛋中抗菌药物的残留状况未见有综合性、系统性的归 纳总结。本文总结了2001-2019年国内外文献及2018-2019年我国市场监督管理总局网站中报道的禽蛋抗菌药物残留情况总 结。分析了分药物类别对禽蛋中残留的抗菌药物阳性率及残留量现状,为抗菌药物的监管及食品安全的保障提供参考。     

醋酸棉酚对大鼠孕卵转运和发育的影响

本文观察了醋酸棉酚对大鼠孕卵在输卵管内转运和早期发育的影响。结果表明,棉酚可延缓孕卵转运速度、阻滞和破坏胚胎早期发育,以对后者的作用更为显著。这可能是棉酚对雌性大鼠抗着床作用的重要原因之一。