Protective effect of rebamipide on indomethacin-induced intestinal damage in rats

BACKGROUND AND AIM: We evaluated the effect of rebamipide (2-(4-chlorobenzoylamino)-3-[2(1H)-quinolinon-4-yl] propionic acid), a novel anti-ulcer drug, on indomethacin-induced small intestinal lesions in rats. METHODS: The animals were administered indomethacin (10 mg/kg, s.c.), and they were killed 24 h later. Rebamipide (30-300 mg/kg) was administered p.o. twice, 30 min before, and 6 h after indomethacin. RESULTS: Indomethacin caused hemorrhagic lesions in the rat small intestine, accompanied by an increase in enterobacterial translocation, inducible nitric oxide synthase (iNOS) and myeloperoxidase (MPO) activities, as well as thiobarbituric acid (TBA) reactants, and these changes were significantly prevented by the supplementation with 16,16-dimethyl prostaglandin E2 (dmPGE2; 10 microg/kg, i.v.) or the pretreatment of animals with the antibiotic ampicillin. Treatment of the animals with rebamipide dose-dependently prevented the development of intestinal lesions, and this effect was mimicked by i.v. administration of superoxide dismutase (SOD: 3000 U/kg) + catalase (CAT: 5000 U/kg). The protection by rebamipide was accompanied by a significant suppression of the increase in both MPO and iNOS activities, and a complete inhibition of the increase in TBA reactants, while SOD + CAT significantly inhibited the increase of MPO activity and TBA reactants, but not iNOS activity. The bacterial translocation following indomethacin was also significantly decreased by either rebamipide or SOD + CAT. CONCLUSION: These results confirmed the importance of enterobacteria and iNOS/NO in the pathogenesis of indomethacin-induced small intestinal lesions, and suggested that rebamipide prevents the development of these lesions, probably by its radical scavenging action.     

依达拉奉联合克林澳治疗急性脑梗死的疗效观察

目的观察依达拉奉联合克林澳（马来酸桂哌齐特）注射液治疗急性脑梗死的临床疗效。方法选择2009年1月—2011年2月在我院神经科住院的200例急性脑梗死患者,随机分为治疗组（100例）及对照组（100例）。两组患者除给予常规治疗外,治疗组给予依达拉奉注射液30mg,2次/d及克林澳注射液320mg,1次/d;对照组100例给予依达拉奉注射液30mg,2次/d。两组患者均治疗14d,观察两组治疗前后疗效、神经功能缺损评分和欧洲脑卒中评分量表（ESS）评分的变化并进行对比分析。结果两组患者治疗后临床疗效、神经功能缺损评分和ESS评分的变化差异有统计学意义（P〈0.05）。结论依达拉奉联合克林澳注射液治疗急性脑梗死的疗效肯定,能明显改善神经功能的损伤,是一种安全有效的方法,值得临床推广应用。     

Antioxidant activity of newly discovered lineage of marine actinobacteria

ObjectiveTo investigate the antioxidant activity of marine actinobacteria.MethodsThe content of total phenolics, the level of antioxidant potential by DPPH radical scavenging activity, metal chelating activity, FRAP method, β carotene assay and NO scavenging activity in extract were determined.ResultsIn all the methods the extract exhibited good scavenging activity except NO scavenging activity. The IC₅₀ values of marine actinobacteria extract on DPPH radical were found to be 41.09 μg/mL. The zone of color retention was 12 mm in β-carotene bleaching assay. DNA protective efficiency of the extracts was also studied using UV-photolysed H₂O₂-driven oxidative damage to pBR322. HPLC analysis identified some of the major phenolic compounds in extracts, which might be responsible for the antioxidant potential and cyto-protection. It showed a 100% cytotoxic effect in brine shrimp lethality assay within 10 mins. The novel actinobacteria was identified as Streptomyces LK-3 (JF710608) through 16S rDNA Sequencing.ConclusionsThe results obtained suggest that the extracts bear anti-cancer metabolites and could be considered as a potential source for anti-cancer drug development.     

2商业化废气排放系统在非层流手术间中应用效果评价

目的评价商业化废气排放系统净化手术室空气的效果。方法根据是否开启废气排放系统,随机将手术室分为两组。在开启异氟醚挥发罐后,每30分钟利用顶空采样瓶分别对开启商业化废气排放系统（n=10）扣未开启该系统（n=10）的手术室内空气进行采样,直至挥发罐开启后180分钟。利用安捷伦6890型顶空色谱,测定吸入麻醉药物（异氟醚）浓度。结果在开启废气排放装置的手术间内,残余异氟醚浓度显著低于对照手术间（P〈O．05）,但仍高于美国国立职业安全与健康研究所推荐的安全线（2ppbv）。结论尽管在非层流手术间内应用商业化废气排放系统可以有效降低手术间内残余吸入麻醉药浓度,但尚不能使其降至推荐的安全线以内。