人脑胶质瘤PET成像中11C-蛋氨酸摄取与LATl和4F2hc表达的关系

背景与目的：以“C-蛋氨酸（11C-MET）为示踪剂的正电子发射体层成像（PET）可为脑肿瘤的氨基酸代谢提供重要信息,大型氨基酸转运载体1（LATl）和细胞表面抗原4F2重链（4F2hc）所形成的LATl／4F2hc复合物是包括蛋氨酸在内的大型、中性氨基酸的主要转运载体．本研究旨在探讨人脑胶质瘤中11C-MET摄取量与LATl和4F2hc表达的关系。方法：对30例新诊断的脑胶质瘤患者行11C-METPET检查．计算11C-MET最大标准化摄取值（SUVmax）;采用免疫组化方法检测LATl和4F2hc在相同脑胶质瘤标本中的表达;分析11C-METSUVmax、LATl和4F2hc表达水平与胶质瘤临床病理学特征的关系以及三者之间的关系。结果：HC．METSUVmax、LATl和4F2hc表达均随胶质瘤病理级别的升高而明显增强（P=0．000。P=0．028。P=0．003）,在高恶性度胶质瘤中11C．METSUVmax、LATl和4F2hc表达也均明显强于低恶性度胶质瘤（P=0．000．P=0．032．P=O．004）：LAT1和4F2hc表达之间存在明显正相关（P=0．036）;uC．METSUVmax与IATl表达也存在明显正相关（P=0．003）,但与4F2hc表达无明显相关性（P=0．366）。结论：11C-MET摄取量以及LATl和4F2hc表达与脑胶质瘤的病理学特征关系密切．LATl高表达可能是脑胶质瘤11C-MET摄取量增高的一个重要因素。     

Folate Deficiency in Malnutrition, Malabsorption, and During Phenytoin Treatment Diagnosed by Determination of Serine Synthesis in Lymphocytes

Abstract A new method for the detection of folate deficiency by measuring the rate of incorporation of ¹⁴C-formate into serine in isolated lymphocytes has been applied to a group of 24 control subjects and 23 patients. 5 of the patients suffered from malnutrition, 14 had malabsorption and 4 were under treatment with phenytoin.
In 17 of these patients, functional folate deficiency was diagnosed by a decreased rate of serine synthesis in the lymphocytes. 11 cases of folate deficiency were found by measuring the folate concentration of erythrocytes by the L. casei method. The lymphocyte method failed in only one case out of 18.
In the 24 control subjects the average incorporation of ¹⁴C-formate into serine per 10⁹ lymphocytes per 4 h was 9.2 ± 0.4% (range 6.3–14.3 %) of added activity. For 16 patients with functional folate deficiency, the corresponding value was 4.1 ± 0.3% (range 1.0–5.9%). The folate activities of lymphocytes from 12 patients before and after 4 weeks of oral treatment with 15 mg folic acid daily were 3.4±0.3 and 7.5±1.0% respectively. The lymphocytes responded within 4 weeks to deprivation of or treatment with folic acid.
The ¹⁴C-formate incorporation into methionine, RNA sad protein showed the same pattern as the incorporation into serine.     

高效液相色谱法测定甲硫氨酸维B_1注射液的含量

目的建立5μm高效液相色谱法同时测定甲硫氨酸维B1注射液中两组分的含量。方法用KromasilC18柱(250mm×4.6mm,5μm),流动相为1mmol/L的己烷磺酸钠溶液(含0.2%三乙胺,用50%H3PO4溶液调节pH5.0)-甲醇(85∶15),流速为1.0ml/min,检测波长为228nm。结果甲硫氨酸和维生素B1分别在80~723μg/ml与8.4~75.6μg/ml的浓度范围内呈良好的线性关系(r=0.9998与0.9999),两者的平均回收率RSD分别为(99.3±0.5)%与(99.8±0.7)%(n=9)。结论此法快速简便,有较好的精密度和准确度。     

Peptide coincidence in rat superior cervical ganglion

The immunofluorescent localization of substance P (SP), somatostatin and methionine enkephalin has been determined in the rat superior cervical ganglion of the sympathetic nervous system. Immunoreactivity was confined to the norepinephrine-containing post-ganglionic neurons and their processes. Nearly 20% of the postganglionic somata demonstrate dual coincidence in staining for SP-somatostatin, somatostatin-enkephalin, or SP-enkephalin. Almost 10% of the positively stained neurons show a coincident staining of all 3 peptide compounds within their somata. This suggests that multiple neurotransmitter chemicals may be employed by the postganglionic cells of the superior cervical ganglion.     

药物性胆汁淤积型肝病的治疗研究

目的探讨药物性胆汁淤积型肝病的有效治疗方案。方法 217例患者符合药物性胆汁淤积型肝损害的诊断标准。分为SAM+H+UDCA组、SAM+H组、SAM+UDCA组、H+UDCA组、中药组。观察各组患者治疗前及治疗4周后的临床症状、体征,检测血清碱性磷酸酶(ALP)、转氨酶(AST、ALT)、总胆红素(TBil)、r-谷氨酰转肽酶(r-GT)、胆碱酯酶(CHE)等生化指标。采用配对设计t检验方法进行统计学分析。结果疗程满4周后,各组临床症状有明显改善,尤其是瘙痒症状评分明显下降。治疗后,各组主要生化指标血清TBil、ALT、AST、ALP、r-GT均下降,CHE升高。其中以血清TBil下降明显,差异有统计学意义(P0.05)。SAM+H+UDCA组和SAM+H组较其他治疗组总胆红素下降速度快、幅度大。但两组之间治疗后TBil水平差异无统计学意义(P0.05);加用激素组随治疗时间延长,使用激素的副作用发生率增加。结论腺苷蛋氨酸与激素联合应用是治疗药物性胆汁淤积型肝病的较好方法,但需注意激素引起的不良反应。     

Effect of reduced temperature on glycoprotein (Ig, HLA) processing and transport in lymphoid cells

Secretion of Igs and surface expression of HLA antigens was examined in lymphoid cells as a function of temp. Upon reducing the temp from 37 to 20 degrees C a progressive decrease in the secretion of Ig and surface expression of HLA antigens was noted. When the status of the oligosaccharides present on these glycoproteins was examined, conversion of high-mannose [endo-beta-N-acetylglucosaminidase-(Endo H) sensitive] to complex-type (Endo H resistant) oligosaccharides diminished with decreasing temp. At no time was an accumulation of Endo H resistant glycoproteins seen intracellularly. These results show that the phenomenon observed for synthesis and intracellular transport of viral glycoproteins in epithelial cells at reduced temp, namely intracellular accumulation of viral glycoproteins carrying complex sugar moieties, does not necessarily apply to glycoprotein transport in lymphoid cells. A difference in subcellular organization of epithelial and lymphoid cells may be responsible for this discrepancy.     

赖氨酸、蛋氨酸、缬氨酸及苏氨酸在大米粒中的分布及其适宜碾磨度

米粒经手工剥离为“果种皮+糊粉层”、“胚+吸收层”和胚乳三部分,并测定了各部分的蛋白质、赖氨酸、缬氨酸、苏氨酸和蛋氨酸含量。同时观察了实验室加工和工厂加工条件下不同碾磨度对各部分丢失和氨基酸含量的影响。结果显示:果种皮、糊粉层、胚和吸收层等部分虽只占米粒重量的7.27～8.42％,但其蛋白质、赖氨酸、蛋氨酸、缬氨酸和苏氨酸分别占总量的14.05～15.77、29.0～30.5、11.83、16.75和18.4％。在工厂或实验室加工条件下,稻米经碾磨后,米粒中所观察的各成分都有改变,其中赖氨酸含量下降最显著。本文对稻米的适宜碾磨度问题进行了讨论,认为在目前工厂加工条件下,以不超过九二米碾磨度为宜;过分的精白米会降低谷类蛋白质的营养价值。     

Distribution of radioactivity among total myelin protein amino acids following administration of labeled glycine, leucine, or methionine

This paper analyzes the distribution of radioactivity in the different amino acids of brain myelin protein for up to 6-7 weeks after an intracranial pulse administration of radioactive leucine, glycine, or methionine. Results show that there is no significant accumulation or reutilization of protein radioactivity in any form other than the one administered.