化瘀清散汤治疗高血压病40例

高血压是内科临诊中常见病之一,属于中医学"眩晕"、"头痛"范畴.近年来,笔者自拟化瘀清散汤治疗中医辨证为瘀热型高血压病40例,疗效满意,现介绍如下.     

清远市市区蟑螂消长趋势及种群构成调查分析

目的　了解清远市市区各蟑螂种群的消长规律及种群构成,为制定以生态学为基础的综合性蟑螂类防治措施和降低蟑螂的侵害提供科学依据。方法　在 2002~2004年,通过粘捕方式,将捕获的蟑螂类进行分类,统计数量。结果　清远市市区蟑螂类以美洲大蠊和德国小蠊为优势种群,其次分别是澳洲大蠊、褐斑大蠊和黑胸大蠊。2003~2004年清远市市区几种主要蟑螂类的监测数量除黑胸大蠊外,其余均有明显下降的趋势。结论　清远市市区蟑螂类防治情况有了明显改观,防治应提倡综合治理方针策略与加强市民的公共卫生意识相结合。     

Osmotic lysis of tumor spill in ovarian cancer: A murine model

OBJECTIVE: Our purpose was to determine, in the murine model, whether human ovarian cancer cells injected intraperitoneally are subject to osmotic lysis by peritoneal lavage with sterile water, thereby decreasing the establishment of peritoneal implants. STUDY DESIGN: Preliminary experiments on six nude mice determined that the injection of 20 million cells of the SKOV-3 cell line reliably leads to the establishment of intraperitoneal tumor xenografts in the mice within 60 days. Four other nude mice functioned as sham controls undergoing peritoneal lavage with 3 to 4 ml of saline solution or sterile water to determine any adverse effects from the lavage alone. Subsequently, 36 nude (nu/nu) mice were injected intraperitoneally with 1 ml of the SKOV-3 cell line at a concentration of 20 million cells per milliliter. Alternate mice then underwent intraperitoneal lavage with either 3 to 4 ml of normal saline solution (control group) or sterile water (study group). The mice were followed up until tumor growth caused a moribund status or until 60 days after injection and then were killed. At necropsy the number and size of tumor nodules were recorded, and each mouse was assigned a composite tumor score. Statistical comparison used the χ² or Fisher's exact test for discrete variables. Time to failure analysis used the Kaplan-Meier method. RESULTS: Tumor growth occurred in 35 of 36 (97%) of the mice during the study period. In the first 30 days 89% of the saline solution group grew clinically visible tumor compared with 55% of the water group (p = 0.03). Ascites developed more frequently in the water group than in the saline solution group. The median tumor scores at death were significantly higher for the water group versus the saline solution group. Survival time, as determined by the time from injection until moribund status, was worse for the water group (p = 0.002). CONCLUSIONS: Intraperitoneal lavage with sterile water did not offer protection against the establishment of xenografts after the intraperitoneal injection of human ovarian cancer cells in the nude mouse model. (Am J Obstet Gynecol 1996;175:1489-92.)     

中华眼镜蛇毒纤溶组份的纤溶活性及其机制研究

目的：研究中华眼镜蛇（NajaNajaatra）蛇毒中单一纤溶活性组份对人纤维蛋白和纤维蛋白原的降解作用及其机制。方法：使用剩余可凝纤维蛋白原测定法和酶联纤维蛋白原溶解试验研究该纤溶活性组份对人纤维蛋白原的降解作用；使用纤维蛋白平板法和酶联纤维蛋白溶解试验研究它对人纤维蛋白的降解作用；对它的纤溶机制研究使用的是纤维蛋白溶解酶原激活物活性测定法。结果：该活性组分对人纤维蛋白和纤维蛋白原有直接的降解作用。其活性大约是粗毒的10倍。该活性组份仅降解纤维蛋白原的Aa链，对它的印链和了链没有作用。该组分对人纤维蛋白溶解酶原没有激活作用。结论：从中华眼镜蛇毒中提取的单一纤溶活性组分是一种新的能够直接降解人纤维蛋白和纤维蛋白原的酶类。     

眶上神经的应用解剖学研究

对１８具（３６侧）正常人的眶上孔（或切迹）及眶上神经进行了观察和测量。主要结果：１．眶上孔（或切迹）以位于眶上缘内中１／５交界处的多见（４８．７８％）。２．两侧为眶上孔的多见（５５．５６％）。３．眶上孔的形状以椭圆形多见（７８．２６％）。４．其通行的结构由外向内均为Ｎ。Ａ。ｖ。并对眶上神经的分支及其有关结构进行了观测，对有关神经卡压问题进行了讨论。     

An in vitro model for purging of tumour cells from ovarian tissue

BACKGROUND: Cryopreservation and autografting of ovarian tissue may preserve fertility after cancer treatment, but could be hampered by tumour cell contamination. Epithelial tumour cell lysis can be obtained with cytotoxic T cell retargeting through the bispecific antibody BIS-1, with combined affinity for the T-cell receptor and epithelial glycoprotein-2 (EGP-2). Our aim was to study the concept of tumour cell purging in the setting of a suspension of ovarian tissue. METHODS: Human ovarian tissue was brought into suspension by mechanical and enzymatic treatment. Cells of the MCF-7 breast cancer cell line and activated human lymphocytes were co- incubated for 4 h with or without BIS-1 and with or without ovarian suspension. After incubation, MCF-7 cell death and cell growth were evaluated by fluorescent cell detection and MTT assay, respectively. Ovarian tissue morphology was evaluated immunohistochemically. RESULTS: MCF-7 cell death and cell growth inhibition increased with increasing ratios of lymphocytes to MCF-7 cells. BIS-1 addition gave further augmentation, with a maximum depletion of growing MCF-7 cells of 89% (SD 11%) versus without BIS-1, 23% (SD 15%; P < 0.001). Follicles remained morphologically intact. CONCLUSIONS: Purging of added epithelial tumour cells from ovarian tissue with BIS-1 is possible in vitro. Morphologically, follicles remain intact after this procedure. This method may contribute to safer replacement of ovarian tissue in female cancer survivors.     

Corneal epithelial cellular dysfunction from benzalkonium chloride (BAC) in vitro

PURPOSE: To investigate the functional and morphological toxicity of benzalkonium chloride (BAC) on corneal epithelial cells in vitro. METHODS: Primary corneal epithelial cells were cultured from rabbit cornea. Corneal epithelial cells containing radioactive 51Cr were exposed for 5 min, 10 min, 30 min and 60 min to concentration of BAC 0.001%, 0.005%, 0.01%, 0.05% and 0.1%. Control cells were treated with phosphate buffer solution alone. 51Cr release from epithelial cells into the supernatant was used as an index of epithelial cell lysis. Cell detachment (index of cell dysfunction) was analysed by measuring 51Cr activity in the supernatant and wash fluid. Morphological cell damage was investigated with transmission electron microscopy. RESULTS: With the higher concentration and the longer duration of BAC exposure, corneal epithelial cell lysis was increased significantly (P < 0.05). Cells showed severe damage at BAC concentration over 0.05% during 5 min of exposure. Cell dysfunction appeared markedly at BAC concentrations of 0.005% for 30 min of exposure, but decreased with longer exposure times. There was an increase in significant cytoplasmic damage with longer BAC exposure times, although not with a minimal dose of 0.001%. Disrupted cytoplasmic membranes of corneal epithelial cells appeared at the higher BAC concentration of 0.1%, and at the longer exposure time of 30 min with BAC concentration of at least 0.001%. CONCLUSIONS: BAC can induce corneal epithelial dysfunction, which can damage the corneal epithelial barrier. This effect occurs when BAC is used frequently or for periods over 30 min, even when the BAC concentration is low (0.001%).     

Formation of P10 tubular structures during AcMNPV infection depends on the integrity of host-cell microtubules

During infection of insect cells with Autographa californica nucleopolyhedrovirus (AcMNPV), the very late protein P10 forms large fibrillar structures in the cytoplasm and nuclei of infected cells. In this study we have used confocal microscopy in association with a novel P10 antiserum to localise and study P10 in virus-infected cells. P10 was shown to be a component of tubular-like structures that spiralled throughout the cytoplasm and nucleus of AcMNPV-infected cells. These structures were observed to colocalise partly with cortical microtubules. When microtubules were depolymerised with the drug nocodazole, P10 tubules failed to form and the protein appeared concentrated in cytoplasmic foci. For the first time, we provide direct evidence using both antibody pulldown and yeast two-hybrid experiments for the interaction of P10 with host-cell tubulin. It is suggested that this interaction may be a critical factor in AcMNPV-induced cell lysis.     

A review of clinical and laboratory findings and treatment of tumor lysis syndrome

Acute tumor lysis syndrome (TLS) has started to be considered a separate entity or condition associated with bulk tumor treatment. TLS is described as the biochemical disturbances associated with rapid destruction of tumor cells with subsequent synchronised massive release of cellular breakdown products sufficient to overwhelm excretory mechanisms and the body's normal reutilization capacity. The cardinal signs of TLS are: hyperkalemia, hyperphosphatemia, hypocalcemia and hyperuricemia. This review comprehensively discusses the differential diagnosis, pathophysiology and clinical features, possible causal indications for laboratory monitoring and treatment options of TLS.