Immunohistochemical analysis of hepatic interferon alpha-beta receptor level: relationship between receptor expression and response to interferon therapy in patients with chronic hepatitis C

Background/Aims: This study aimed to determine the expression level of interferon alpha/beta (IFN-α/β) receptor in the liver immunohistochemically and evaluate its usefulness in predicting the outcome to IFN therapy in patients with chronic hepatitis C.Methods: The level of IFN-α/β receptor expression was determined in immunoperoxidase-stained pretreatment sections of 55 chronic hepatitis C patients later treated with IFN. We used liver biopsy specimens and mouse monoclonal anti-human IFN-α/β receptor antibody. Quantitative analysis of immunostaining was performed by image analysis software. The level of IFN-α/β receptor was expressed as Unit (U). Sustained responders were patients who showed persistent disappearance of serum HCV-RNA during the 6-month period after treatment, while non-responders showed persistence of viremia after therapy.Results: Positive immunostaining was observed in the cytoplasm of hepatocytes. The mean expression level of hepatic IFN-α/β receptor in sustained responders (2.65±1.11 U, n=15) was significantly (p<0.001) higher than in non-responders (1.61±1.05 U, n=40). A significant decrease in IFN-α/β receptor expression level was observed in patients with advanced liver fibrosis. In patients with low level viremia (pretreatment serum HCV-RNA <1 Meq/ml, n=18), the level of IFN-α/β receptor in sustained responders (2.89±1.12 U, n=11) was significantly (p<0.01) higher than in non-responders (0.93±0.33 U, n=7).Conclusions: Our results suggest that measurement of the level of hepatic IFN-α/β receptor in patients with chronic hepatitis C might be useful for predicting the response to IFN therapy. Resistance to IFN therapy in patients with chronic hepatitis C might be due to low levels of hepatic IFN-α/β receptor.     

Prognostic value of serial serum KL-6 measurements in patients with idiopathic pulmonary fibrosis

Background

The prognostic significance of serial measurements of serum KL-6 levels in patients with idiopathic pulmonary fibrosis (IPF) is unclear; hence, it was assessed in this study.

Anion gap‐opening metabolic acidosis and urinary findings in the early diagnosis of ethylene glycol poisoning: A case report

Causative agent identification is important in the treatment of poisoning. We report the case of a patient who presented with an altered level of consciousness after drinking a fluorescent pink liquid. Upon measuring the anion gap and urinary calcium oxalate level, the patient was diagnosed with early ethylene glycol poisoning.     

2734例社区健康居民血脂水平检测及相关分析

目的:了解本地区20岁以上健康人群血脂水平与饮食结构、年龄及性别的关系.方法:用随机抽样方法,抽取开封市20岁以上社区健康居民2734人,进行问卷调查并检测血脂水平.结果:血清总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)随年龄而增高,但男女之间增加幅度并不平行;高密度脂蛋白胆固醇(HDL-C)随年龄变化不明显.血脂水平的变化与饮食结构的改变有关.结论:倡导合理的饮食结构,坚持体育锻炼,以降低高脂血症,预防心脑血管疾病的发生.     

益气复脉片质量标准研究

目的:本文主要对益气复脉片的质量标准进行了系统的研究,建立了以高效液相色谱法(HPLC)测定五味子醇甲。方法:比色法测定总皂苷的相关有效成分含量测定方法;另采用薄层色谱(TLC)鉴定了麦冬。结果:益气复脉片含总皂苷的平均值为6．61mg。结论:此质量检测方法的分离度、重复性均好,精密度高,简单快速,建议本品质量标准每片五味子甲醇含量不低于1．4mg;总皂苷含量不低于5mg。     

干预前后居民心脑血管病、糖尿病知信行水平分析

目的了解人群对心脑血管病和糖尿病相关知识的掌握情况,评价以社区为基础的以健康教育为手段的干预效果。方法干预Methods为创造社区健康支持环境、家庭动员、家庭行为干预和健康讲座。干预前后采用随机抽样的方法抽取杭州市常住居民,进行心脑血管病糖尿病防治知信行调查,干预前调查798例,干预后调查500例;结果初查显示杭州市六个区的常住居民对心脑血管病和糖尿病防治知信行水平较低;通过一年的综合干预,28项知信行指标均有了显著提高。结论健康促进是控制心脑血管病和糖尿病的重要措施,行为干预的效果优于一般的健康教育,以社区为基础的心脑血管病和糖尿病综合干预在我国是切实可行且行之有效。     

逍遥散加味联合红金消结片对气滞血瘀型乳腺增生患者乳房超声指标及血管生成因子水平的影响

目的 探讨逍遥散加味联合红金消结片对气滞血瘀型乳腺增生患者乳房超声指标及血管生成因子水平的影响.方法 选取2019年11月至2020年12月我院接收的86例气滞血瘀型乳腺增生患者为研究对象,根据治疗方案将其分为对照组与观察组,各43例.对照组给予红金消结片治疗,观察组在对照组基础上加施逍遥散加味治疗.比较两组患者的临床...     

Adverse Tissue Reactions and Metal Ion Behavior After Small‐Head Metasul Hip Arthroplasty: A Long‐Term Follow‐Up Study

ObjectiveTo investigate the long‐term survivorship, incidence of adverse reactions to metal debris (ARMD), and metal ion behavior in patients who underwent small‐head Metasul metal‐on‐metal (MoM) total hip arthroplasty (THA).MethodsBetween February 1998 and September 2003, a retrospective study was performed on 43 consecutive patients (43 hips) who underwent unilateral cementless Metasul MoM THAs at our institution. Of them, 35 patients (nine males and 26 females) who were available for follow‐up more than 15 years after THA were enrolled in this study and underwent metal artifact reduction sequence magnetic resonance imaging (MARS‐MRI) to identify ARMD. The mean age at surgery of the patients was 59.7 years old (range, 31–83). Clinical and radiographic outcomes were evaluated retrospectively. Clinical examinations were conducted using the Harris Hip Score (HHS). Serum cobalt (Co) and chromium (Cr) ion levels and Co/Cr ratio were assessed at different postoperative periods of <5, 5–10, 11–14, and ≥15 years.ResultsThe mean follow‐up period for the 35 patients included was 18.1 years (range, 15–22). The mean HHS significantly improved from 44.6 ± 11.3 points preoperatively to 89.4 ± 7.9 points at the final follow‐up (P < 0.0001). ARMD was found in 20% of the patients using MARS‐MRI. No signs of stem loosening were found clinically or radiographically, whereas cup loosening and ARMD were observed in three patients (9%), for whom revision THAs were performed. The Kaplan–Meier survival rates with revision for any reason as the endpoint were 90.9% at 5 years, 84.8% at 10 years, 84.8% at 15 years (95% CI, 67.1–93.6), and 70.3% at 20 years (95% CI, 43.6–87.0). The survival rates with revision for ARMD as the endpoint were 100% at 5 years, 96.6% at 10 years, 96.6% at 15 years (95% CI, 77.2–99.7), and 80.1% at 20 years (95% CI, 45.3–95.2). Serum Co ion level peaked at 5–10 years after THA, which was significantly higher than that <5 years; however, it decreased to the initial level after 15 years. In contrast, serum Cr ion level significantly increased at 5–10 years and then remained almost constant. Significant differences in Cr ion levels (1.0 vs 2.0 μg/L, P = 0.024) and Co/Cr ratio (1.3 vs 0.9, P = 0.037) were found between non‐ARMD and ARMD patients at >11 years postoperatively.ConclusionOur results suggest that increased Cr ion levels and decreased Co/Cr ratio may be signs of ARMD in patients who underwent small‐head Metasul MoM THA.