拉西地平血浆药物浓度测定的方法学

建立了反相高效液相色谱法测定拉西地平血药浓度的方法，应用Ｎｏｖａ－ｐａｋ Ｃ１８径向加压柱，以甲醇－水（７５：２５）为流动相，检测波长为ＵＶ３００ｎｍ。血样用乙醚提取，拉西地平的平均提取回收率大于７５％，日内，日间变异小于１０％，本方法最低检测浓度为４ｎｇ／ｍｌ。     

Calcium antagonists and endothelial function: Focus on nitric oxide and endothelin

Summary This paper reports on some interactions of calcium antagonists with nitric oxide and endothelin. It reviews evidence showing that the vasorelaxant action of calcium antagonists is facilitated by nitric oxide and describes the mechanism of this modulation. The interaction of calcium antagonists with endothelin is examined considering functions and production of the peptide. Among the functions examined, attention is drawn to the potentiation of responses to vasoconstrictors evoked by low threshold concentrations of endothelin, an action that could be important in pathology. The production of endothelin is increased by a high-salt diet in spontaneous hypertensive stroke-prone rats, this increased production, related to the overexpression of prepro ET-1mRNA, is responsible for cardiovascular hypertrophy and is blunted, in a blood pressure-unrelated manner, by the calcium antagonist lacidipine. At the same dosage regimen, lacidipine inhibits the hypertrophy of the cardiovascular system evoked by a high-salt diet.     

拉西地平片中有关物质的含量测定及初步确证

目的建立高效液相色谱法测定拉西地平片有关物质。方法采用C18色谱柱,流动相：甲醇-水（75：25）;柱温：35℃;检测波长：283 nm;流速：1.0 mL·min……-1。采用UPLC/Q-TOF-MS对有关物质进行质谱定性。结果在该色谱条件下拉西地平与有关物质分离度良好,能有效检测出拉西地平的降解产物及合成中带入的杂质,并经质谱进行初步确证。结论该方法能够简便、灵敏、准确的检测出拉西地平的有关物质。     

拉西地平治疗糖尿病并发高血压及单纯高血压的疗效

目的：观察拉西地平对糖尿病并发高血压和单纯高血压的治疗效果。方法：单纯高血压和糖尿病并发高血压各２５例,服安慰剂 ２ ｗｋ后服用拉西地平２ ｍｇ,如血压未降至正常,则渐增到４,６ ｍｇ或 ８ ｍｇ,ｐｏ, ｑｍ, ｐｃ,共 ６ ｗｋ。结果： ２组治疗 ２ｗｋ后收缩压分别下降达（３．４±０．６）ｋＰａ或（３．５±０．６）ｋＰａ,（Ｐ＜０．０１）;舒张压分别下降达（２．０±０． ３） ｋＰａ或（２． ２± ０． ４） ｋＰａ（ Ｐ＜ ０． ０５）。治疗 ４ ｗｋ及６ｗｋ,均显示收缩压进一步下降;舒张压均维持原疗效;治疗６ｗｋ期间对心率、血糖、血脂均无显著影响。结论：拉西地平治疗单纯性或糖尿病并发高血压均有较好疗效。