甲基硝基亚硝基胍对日本血吸虫成虫培养细胞骨架作用的研究

目的 通过观察N－甲基－N－硝基－N－亚硝基胍（MNNG）诱导日本血吸虫培养细胞后骨架的改变,研究MNNG对培养细胞转化、增殖的作用。方法 将日本血吸虫成虫培养细胞接种于小盖玻片上,培养1周时用浓度为3mg/L的MNNG处理48h,然后用RPMI－1640含20％小牛血清附加常量抗菌素的培养基培养2周,再换用RPMI－1640含5％小牛血清的培养基继续培养,用考马斯亮兰染色法和鬼笔环肽荧光染色法显示培养细胞骨架。结果 MNNG诱导后,在培养第4、5周时部分培养细胞骨架排列紊乱,微丝集聚于细胞边缘。结论 MNNG有一定诱导日本血吸虫成虫培养细胞转化、促进其分裂的作用。     

普罗布考对1-甲脲乙醇酸酐诱导HK-2细胞表达纤维化相关因子的影响

目的研究降脂药普罗布考对小分子尿毒素1-甲脲乙醇酸酐诱导纤维化相关因子在人肾小管上皮细胞表达的影响。方法 HK-2细胞分3组培养,正常对照组：不加干预因素;1-甲脲乙醇酸酐组：培养基中加入0.5mmo1/L1-甲脲乙醇酸酐;1-甲脲乙醇酸酐＋普罗布考组：培养基中加入0.5mmo1/L1-甲脲乙醇酸酐和20μmol/L普罗布考。各组细胞培养48h后用免疫细胞化学、RT-PCR检测TGF-β1、CTGF和FN,并用流式细胞仪对以上因子作定量检测,以观察其在HK-2细胞中的表达。结果在1-甲脲乙醇酸酐刺激后,免疫细胞化学染色HK-2细胞质中着色,显示TGF-β1、CTGF和FN均有表达。正常对照组HK-2细胞质中无着色。测定结果显示1-甲脲乙醇酸酐组TGF-β1、CTGF和FN显著高于正常对照组（P〈0.01）,加入普罗布考后,表达显著下降（P〈0.05）。结论 1-甲脲乙醇酸酐能促进纤维化相关因子在肾小管上皮细胞中表达;普罗布考能抑制1-甲脲乙醇酸酐诱导纤维化相关因子在肾小管上皮细胞的表达。     

隐形眼镜护理液杀灭微生物效果及防腐性能的试验观察

目的了解隐形眼镜护理液的杀菌效果及防腐性能。方法采用悬液定量杀菌试验方法观察护理液对各种微生物杀灭效果及防腐作用。结果用含聚六亚甲基双胍1.8μg/ml护理液原液作用1.5 h,对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌的杀灭率均为100%,对茄科镰刀霉菌的杀灭率为99.95%。在护理液内分别加入含菌量为1.0×105~1.0×106cfu/ml的大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌,保持14 d时细菌减少率>99.9%;于14 d后再加入终浓度为1.0×104~1.0×1065cfu/ml试验菌保持到第28 d减少率>99.9%。结论该隐形眼镜护理液具有良好的杀菌性能和防腐作用。     

一种复方消毒剂杀菌效果及毒性的实验观察

试验用复方消毒剂是以甲壳素、溶菌酶及聚六亚甲基胍等为杀菌成分。为观察其杀菌效果与毒性,采用载体定量杀菌试验和动物毒性试验进行了实验室研究。结果,以含聚六亚甲基胍3000 mg/L该消毒液对布片上大肠杆菌、金黄色葡萄球菌作用5 m in,对布片上白色念珠菌作用10 m in,杀灭率均达99.99%。将该消毒液原液密封置于37℃放置90 d,聚六亚甲基胍下降率为4.4%,对金黄色葡萄球菌和白色念珠菌杀灭效果基本不下降。菌悬液内含体积分数50%小牛血清对该消毒剂杀菌效果无明显影响。该消毒剂对小鼠经口LD50>5000 mg/kg,蓄积系数K>5;该消毒液原液对家兔皮肤刺激试验积分为0,眼刺激试验积分为3.0;该消毒液对小鼠骨髓嗜多染红细胞微核试验为阴性,精子畸形试验结果为阴性。该消毒剂原液浸泡72 h,对不锈钢、铜无腐蚀,对碳钢和铝基本无腐蚀性。结论,该复方消毒剂对细菌繁殖体和真菌杀灭效果较好;属实际无毒级,弱蓄积毒性;对皮肤属无刺激性,对眼睛为轻度刺激性;性能稳定,腐蚀性较低。     

MNNG加吐温20和维生素D3对大鼠前胃的影响

目的　研究胃癌发生、发展过程中，间质组织中血管反应与肿瘤的关系．方法　经水摄入Ｎ－ 甲基－ Ｎ１ － 基－ Ｎ － 亚硝基胍（ ＭＮＮＧ）和表面活性剂（ 吐温２０） 诱发大鼠肿瘤，饲料中添加维生素Ｄ３（ＶｉｔＤ３） 对肿瘤进行预防． 每８ ｗｋ 处死大鼠７ 只，３２ ｗｋ 实验结束．结果　ｗ ｋ １６ 实验组大鼠前胃鳞状上皮形态主要以血管密度增加、血管内皮细胞增生，３２ ｗｋ（ ＭＮＮＧ） ＋ 表面活性剂和ＶｉｔＤ３ 组肿瘤发生率高于ＭＮＮＧ 组（１０ ％ ） ，肿瘤发生率为６５ ％ ．结论　该组动物诱癌实验表明，肿瘤形成前主要以血管新生、血管ＥＣ 增生为主的病理性变化．     

口卫士新型亮白健齿片活性与质量的研究#br#

目的 评价口卫士亮白健齿片祛牙斑、抑菌和安全性和建立本品的质量控制方法。方法 采用陈旧性牙斑牙为模型,测试祛牙斑的清除力;采用金色葡萄球菌、大肠埃希菌、肺炎双球菌和白念珠菌为菌种,验证本品的抑菌能力;采用急毒验证其安全性;采用分光光度法分别测定醋酸氯苯胍葶和过碳酰胺的含量。结果 亮白健齿片可在刷洗约2min后逐渐除去色斑牙的色斑,随着刷洗次数增多,色斑清除越明显;对金色葡球菌和大肠埃希菌的最低抑菌浓度(minimal inhibititory concentration, MIC)为6.25μg/mL;最大给药量18g/kg;醋酸氯苯胍葶和过碳酰胺分别在5.50~17.40μg/mL和0.12~0.45mg/mL范围内线性关系良好,线性方程分别为y=0.0508x-0.0729(R²=0.9993)和y=1.7245x+0.0185(R²=0.9991),醋酸氯苯胍葶的重复性和中间精密度分别为0.65%和0.72%,平均回收率为99.60%;过碳酰胺的重复性和中间精密度分别为0.29%和0.41%,平均回收率为100.4%。结论 口卫士亮白健齿片不仅能清除牙釉质表面色素沉着,抑制口腔细菌,且质量稳定,安全性良好。     

Expression of caspase-9 affected by AG on retina of rats with chronic IOP elevation

AIM: To study caspase-9 expression on rat retina in the process of chronic elevation of IOP and the changes with the application of amino guanidine (AG), thus to investigate the potential protective function of AG to rat retina with chronic elevation of IOP. METHODS: Immunohistochemistry, RT-PCR and Western blot were used to observe retinal morphology and the expression of caspase-9 at different time points of rat with chronic IOP elevation, both affected and not affected by the application of AG. ·RESULTS: Compared with control group, as time passed retina of experimental group gradually had detectable morphological changes. On 21^st day of chronic IOP elevation, retinas became thinner and the quantity of retinal ganglion cells (RGCs) decreased; caspase-9 expression increased, consistent with the morphological changes. The group using AG presented relatively smaller morphology changes and less expression of caspase-9. CONCLUSION: Apoptosis-related gene caspase-9 plays a part in the process of chronic IOP elevation; AG protects retina by down-regulating expression of caspase-9.     

Partial specific volume and interaction of glycinin with solvent components in urea and guanidine hydrochloride

The interaction of glycinin with urea/guanidine hydrochloride was investigated by determining the apparent partial specific volume of the protein in these solvents. The apparent partial specific volume was determined under both isomolal and isopotential conditions. The preferential interaction parameter with solvent components had values of 0.10 and 0.11 g urea and guanidine hydrochloride respectively per g of protein. In both the cases the interaction was not preferential with water. The total binding of urea/guanidine hydrochloride to glycinin was calculated. Excellent correlation between the calculated value and the total number of peptide bonds and aromatic amino acids residues of protein was observed. The changes in volume upon transferring glycinin from dilute salt solution to urea and guanidine hydrochloride solution were also calculated.     

Developmental changes in nerve growth factor level in rat serum.

In serum, nerve growth factor (NGF) forms a complex with alpha 2-macroglobulin (alpha 2M), which formation inhibits the immunoreactivity between NGF and its antibodies. For measuring the serum level of NGF, it is thus necessary to liberate NGF from the NGF-alpha 2M complex and prevent reformation of such complex. The pretreatment of rat serum with 1 M guanidine hydrochloride for a few hours and operation of the enzyme immunoassay (EIA) in the presence of guanidine hydrochloride provided a reliable means for determination of the NGF level in serum. By this procedure we followed the serum NGF level in rats developmentally. It increased from prenatal day 2 to postnatal day 5 and decreased slightly at postnatal week 3, thereafter remaining constant throughout adulthood. In pregnant rats, the NGF level in serum increased threefold to fivefold before birth and then decreased rapidly. These data suggest that serum NGF level may reflect the demand for this molecule during establishment of the peripheral nervous system.     

Synthesis of Novel Aminopropylguanidine Derivatives with Potential Antihypertensive Activity

Pharmaceutical Research -